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Citations for Qiagen :
451 - 500 of 3116 citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was extracted from VSV-SARS-CoV-2 mutant viruses using RNeasy Mini kit (Qiagen), and S was amplified using OneStep RT-PCR Kit (Qiagen) ...
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bioRxiv - Developmental Biology 2021Quote: ... 106 cells were then transfected with 2 μg of DNA using Effecten® transfection reagent (Qiagen) according to the manufacturer’s instructions at a 1:10 DNA/Effecten® ratio ...
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bioRxiv - Microbiology 2021Quote: ... and gel purified on a 2% agarose gel using Qiagen Gel Purification Kit (Qiagen, Frederick, MD). After quality check using 2100 Bioanalyzer and DNA 1000 chip (Agilent Technologies ...
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bioRxiv - Systems Biology 2022Quote: ... 2 mL cultures were immediately added to centrifuge tubes containing 4 mL RNAprotect Bacteria Reagent (Qiagen), vortexed for 5 seconds and incubated at room temperature for 5 min ...
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted from the 2 µm filters using the DNeasy PowerWater kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR of cDNA samples was performed using 2× Rotor-Gene SYBR Green PCR Mastermix (Qiagen) and Rotor-Gene Q real-time PCR cycler (Qiagen).
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bioRxiv - Molecular Biology 2020Quote: Cells from 1.0 ml of culture were mixed with 2 ml of RNA protect reagent (Qiagen). The mixture was pelleted at 4000 rpm for 10 minutes and then discard the supernatant ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was then incubated with 2 ml Ni-NTA Superflow resins (Cat No.30430, QIAGEN) for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... Viral RNA was extracted from VSV-SARS-CoV-2 mutant viruses using RNeasy Mini kit (Qiagen), and S was amplified using OneStep RT-PCR Kit (Qiagen) ...
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bioRxiv - Cell Biology 2021Quote: ... genomic DNA was extracted from 2 × 106 cells per duplicate using DNeasy Blood & Tissue Kits (QIAGEN) and sheared to 100-300 bp with a sonicator ...
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bioRxiv - Genomics 2021Quote: cfDNA was extracted from 2-4 mL of plasma using the QIAsymphony liquid handling robot (Qiagen). cfDNA concentration was determined using Qubit double-strand molecular probes kit (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... The homogenate was then centrifuged at full speed for 2 min in QIAshredder column (Qiagen, 79656). RNA was extracted from the flow-through using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... Viral RNA was extracted from VSV-SARS-CoV-2 mutant viruses using RNeasy Mini kit (Qiagen), and S was amplified using OneStep RT-PCR Kit (Qiagen) ...
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bioRxiv - Genomics 2021Quote: ... PCR products were run on a 2 % gel and purified using QIAquick Gel extraction kit (Qiagen).
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bioRxiv - Bioengineering 2021Quote: ... size selected on a 2% agarose gel and purified with a QIAquick Gel Extraction Kit (Qiagen) as per manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... for 1h at 37C and purified from a 2% agarose gel (QIAquick Gel Extraction Kit, Qiagen). In parallel ...
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bioRxiv - Immunology 2022Quote: ... Viral RNA was extracted from VSV-SARS- CoV-2 mutant viruses using RNeasy Mini kit (Qiagen), and the S gene was amplified using OneStep RT-PCR Kit (Qiagen) ...
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bioRxiv - Immunology 2022Quote: ... genomic DNA from 2×107 cells was extracted by DNeasy blood & tissue kit (Qiagen, Cat.69504) and sgRNA cassettes were PCR amplified for illumina NGS 53.
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bioRxiv - Plant Biology 2022Quote: ... The supernatant was applied to a 2-ml column of Ni-NTA-agarose (Qiagen, Hilden, DE) at a flow rate of 1 ml min−1 ...
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bioRxiv - Molecular Biology 2022Quote: ... rotated at 4°C for 1.5-2 hr in 0.75 mL equilibrated Ni-NTA Agarose (Qiagen), followed by packing pre-equilibrated Poly-Prep Chromatography Columns (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... RNA was extracted from the Caco-2 cell lines using a RNeasy Mini Kit (QIAGEN SA) with a DNase I step to eliminate DNA contaminants ...
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bioRxiv - Neuroscience 2024Quote: Total RNA from iPSC-derived microglia (Supplementary Table 2) was extracted using RNeasy Plus kit (Qiagen). Library construction and sequencing were performed by Novogene ...
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bioRxiv - Immunology 2023Quote: DE genes between clusters 2 and 4 were fed to Ingenuity Pathway Analysis software from Qiagen. Enrichment pathways were sourced from Ingenuity core enrichment pathways.
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bioRxiv - Genetics 2023Quote: ... PCR amplicons were resolved by 2% agarose gel electrophoresis and gel-purified (QIAGEN Gel Extraction Kit). Libraries were quantified by qPCR using the NEBNext Library Quant Kit (NEB) ...
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bioRxiv - Pathology 2023Quote: ... at 35 Hz for 2 minutes with a sterile stainless steel 0.5 mm metal bead (Qiagen). Homogenized samples underwent depletion of host and bacterial ribosomal RNA using a modified previously published protocol (40) ...
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bioRxiv - Microbiology 2023Quote: ... each cell cultures (4ml) are treated with 2 volumes of RNA Protect Bacteria reagent (Qiagen, Switzerland) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µg of purified genomic DNA was then bisulfite converted using the EpiTect Bisulfite Kit (Qiagen). PCR was performed on bisulfite-treated gDNA using HotStarTaq DNA polymerase (Qiagen) ...
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bioRxiv - Genomics 2023Quote: ... PCR 2 products were purified via gel extraction using the Qiaquick Gel Extraction Kit (Qiagen #28706X4) and prepared for sequencing as described in Illumina’s library denaturation and dilution manual ...
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bioRxiv - Cell Biology 2023Quote: ... The cells sediment was added to 2 mL Red Blood Cells Lysis Solution (Qiagen, Duesseldorf, Germany) at room temperature for 5 min to remove red blood cells ...
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bioRxiv - Cell Biology 2023Quote: ... Take 2 μg of template DNA and bisulfite treat according to the EpiTect Bisulfite Kit (QIAGEN) manual ...
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bioRxiv - Plant Biology 2023Quote: ... Around 2 micrograms of DNA was used for DNA bisulfate conversion by EpiTect Bisulfate kit (QIAGEN). The converted DNA served as a template for amplification ...
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bioRxiv - Biochemistry 2023Quote: PCR products were purified from 2% agarose gel and extracted by QIAquick Gel Extraction Kit (Qiagen) following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2024Quote: ... at 55 °C for 2 hr before recovered using a miniElute PCR purification kit from Qiagen. ChIP-qPCR was performed using primers listed in Supplementary Table 5 and PowerUp SYBR green master mix (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... Samples were transferred to a 2 ml tube with 0.5 mm glass beads (Qiagen, Germantown, Maryland) and bead beat for 10 min at maximum speed on a Vortex-Genie 2 (Scientific Industries ...
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bioRxiv - Synthetic Biology 2024Quote: ... The soluble fraction was loaded onto a column containing 2 mL of Ni-NTA resin (Qiagen) previously equilibrated with 20 mL lysis buffer ...
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bioRxiv - Microbiology 2024Quote: ... The pellet was resuspended in 2 volumes (g pellet:ml volume) of RNA protect Tissue (Qiagen, Germany) and homogenised by passing 3 times through a needle (26G) ...
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bioRxiv - Evolutionary Biology 2024Quote: Total SARS-CoV-2 RNA was extracted by using the Analytik Jena Kit (QIAGEN, Hilden, Germany), followed by reverse transcription into cDNA with a high capacity cDNA reverse transcription kit (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2024Quote: ... and dact1/2-/-fish were collected and RNA extractions were performed using RNeasy Mini Kit (Qiagen). cDNA was generated using High Capacity cDNA Reverse Transcription Kit (ThermoFisher) ...
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bioRxiv - Neuroscience 2024Quote: ... Supernatant was incubated for 2 h at 4°C while rotating with Glutathione superflow beads (Qiagen) for GST-tagged CMK-1 variants or nickel-nitrilotriacetic acid beads (Ni-NTA ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from about 2-4×106 cells using the RNeasy Mini Kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... KCl (to a final concentration of 0.3 M) and 2 ml of Ni-NTA beads (Qiagen) were added and incubated at 40 °C for 1.5 h ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and 2% Triton-X 100) and lysed with 200 μL of AL lysis buffer (Qiagen, 19075). DNA was precipitated by adding 1 mL of 100% ethanol supplemented with 100 mM of sodium acetate ...
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bioRxiv - Genomics 2020Quote: ... purified nucleic acids were treated with 1-5 μl 1 mg/ml RNaseA (Qiagen) for 45 minutes at 37 °C to degrade RNA ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were maintained in sphere-forming cultures for 2 weeks prior to RNA collection (RNAeasy Kit, Qiagen). TGFB1 was replenished when placing mOSE cells in sphere-forming conditions.
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bioRxiv - Molecular Biology 2022Quote: ... Each reaction of the HRM assay included 6.25µl of Type-IT 2 x HRM buffer (QIAGEN, Germany), primers specific for the uidA E.coli housekeeping gene and molecular grade water was added to make a final volume of 12.5µl ...
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bioRxiv - Microbiology 2022Quote: ... 2×109 bacteria were pelleted and DNA was extracted using a DNeasy Blood and Tissue kit (Qiagen). The optional RNase step in the protocol was performed to remove contaminating RNA ...
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bioRxiv - Systems Biology 2020Quote: ... Cell pellets were transferred to 2 ml polypropylene tubes and disrupted twice using a TissueLyser II (Qiagen), adding the same volume of acid-washed glass beads (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting amplicon pool was further purified from a 2% agarose gel (QIAquick Gel extraction Kit Qiagen) with GelRed staining and eluted in TE (10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was incubated for ~2 h rotating at 4°C with Ni-NTA Resin (Qiagen, 1018244) that had been washed with wash buffer (20mM Tris HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2022Quote: 2 x 106 cells were harvested and RNA was purified using Qiagen RNeasy Mini Kit (Qiagen, #74104) using manufacturer protocol ...