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Citations for TianGen Biotech :
51 - 100 of 563 citations for rno mir 137 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The RNA prep pure kit and FastKing RT Kit for RNA extraction and reverse transcription were purchased from Tiangen Biothch Co. ...
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bioRxiv - Neuroscience 2020Quote: ... and then reverse transcribed using a ReverTra Ace qPCR RT Master Mix Kit (TOYOBO, Japan) and a miRcute Enhanced miRNA cDNA First-Strand Synthesis Kit (TIANGEN) according to the manufacturers’ protocols ...
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bioRxiv - Plant Biology 2020Quote: ... First-strand cDNA was synthesized using a Fast Quant RT Kit with gDNA Eraser (Tiangen). Genomic DNA was isolated from transgenic leaf tissues using the DNeasy Plant Mini Kit (Qiagen) ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was obtained according to the instructions of a TIANScript II RT kit (KR107, TIANGEN). The expression of transcripts of the target gene was measured by using a LightCycle® 96 instrument (Roche ...
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bioRxiv - Cancer Biology 2019Quote: ... and the Q-PCR kit was purchased from Tiangen Biotech Co. ...
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bioRxiv - Microbiology 2021Quote: Samples of RNA subjected to RNA-seq analysis used the FastQuant RT Kit with gDNase (TIANGEN) for first-strand cDNA synthesis ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription with ZIKA virus specific primer (Rev-AAGTGATCCATGTGATCAGTTGATCC) was performed using FastQuant RT Kit (Tiangen). Real-time PCR was done on 7900HT (ABI ...
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bioRxiv - Pathology 2020Quote: ... according to the manufacturer’s instructions and cDNAs were reverse-transcribed using the FastKing RT Kit (TIANGEN). The cDNA samples were amplified by Real-time quantitative PCR (RT-qPCR ...
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bioRxiv - Developmental Biology 2021Quote: ... it was performed in accordance with the manufacturer’s instructions using FastQuant RT Kit (With gDNase) (Tiangen). Relative mRNA levels of each gene were analyzed according to the comparative Ct method and were normalized to the expression of GAPDH ...
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bioRxiv - Genetics 2019Quote: ... the PCR amplicons were purified using Gel Extraction kit (TIANGEN). A total of 200 ng of the purified PCR amplicon was denatured and slowly reannealed to facilitate heteroduplex formation ...
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bioRxiv - Biochemistry 2021Quote: ... An aliquot of 0.5 μg RNA were reverse transcribed to cDNA by FastKing RT Kit (KR116-02, Tiangen). cDNA samples were used as templates to perform real- time quantitative PCR with the power SYBR green mix (Abm ...
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bioRxiv - Biochemistry 2019Quote: ... the first step product was converted to cDNA using FastKing RT Kit (With gDNase) (TIANGEN BIOTECH, BeiJing, China). Then ...
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ARID1a protects against steatosis and insulin resistance via PPARalpha-mediated fatty acid oxidationbioRxiv - Cell Biology 2019Quote: ... DNA was purified using a PCR Purification Kit (Tiangen,Beijing,China) and subsequently analyzed by qPCR using primers located on the Acox1 and Cpt1a promoter.
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bioRxiv - Cancer Biology 2022Quote: ... RT–qPCR was performed via a miRcute plus miRNA qPCR kit (SYBR Green) (Tiangen Biotech Co., Ltd., Beijing, China) on an ABI 7500 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... 2 ug RNA per sample were used to reverse transcript the cDNA by QuantScript RT Kit (TIANGEN Biotech, KR103). 2 x iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Genomics 2020Quote: ... China) and first-strand cDNA was synthesized with 1µg RNA using a FastQuant RT Kit (with gDNase) (TIANGEN, Beijing, China). Gene-specific primers were designed using Primer-BLAST software (National Center for Biotechnology Information ...
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bioRxiv - Plant Biology 2022Quote: ... gDNA buffer was added into total RNA to remove DNA impurity and then the purified RNA was used as a template to synthesized cDNA using a FastKing RT Kit (TIANGEN). The RT-qPCR assays were performed on qTOWER3 Real-Time PCR thermal cyclers (Analytik Jena ...
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bioRxiv - Immunology 2020Quote: ... PCR products underwent agarose electrophoresis and gel-purification (Universal DNA purification kit, Tiangen). The amount of PCR products was determined by OD 260 absorption value ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were merged and purified by using TIANquick Midi Purification Kit (TIANGEN). The NGS libraries were prepared by using VAHTS Universal DNA Library Prep Kit for Illumina (Vazyme ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from cultured cells by using TRIZOL reagent and 500 ng to 2 μg RNA was reversely transcribed into first-strand cDNA by FastKing RT Kit (Tiangen, KR116). qPCR analysis was performed with Mastercycler Realplex2 (Eppendorf ...
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bioRxiv - Genetics 2024Quote: ... First-strand cDNA was synthesized from 2 μg of DNase I-treated total RNA using the FastKing RT kit (TIANGEN, KR116). qRT-PCR was performed using the ChamQ Universal SYBR qPCR Master Mix (Vazyme ...
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bioRxiv - Cancer Biology 2023Quote: ... The PCR products were extracted by a gel extraction kit (TIANGEN, Catalog # DP209-03). Data analysis was performed using MAGeCK.
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bioRxiv - Genetics 2022Quote: ... First-strand cDNA synthesis from the total RNA was performed by using the FastKing RT kit (with gDNase) (TianGen Biotech Co, Beijing). The expression analyses were performed using the primer set (F-GGACTTCCCTTGGATCCTGC and R-ACCGACAATCATGTCCGCAT) ...
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bioRxiv - Developmental Biology 2020Quote: ... All RT-qPCR assays were performed in an ABI 7500 System with the SuperReal PreMix Plus kit (SYBR Green) (Tiangen, Beijing, China). All RT-qPCR procedures for each gene were performed in three biological replicates ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA (1 µg) was used as a template for cDNA synthesis with a QuantScript RT Kit (KR103; Tiangen Biotech, Beijing, China). To determine mRNA expression levels ...
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bioRxiv - Bioengineering 2021Quote: ... The quantitative PCR was conducted using the SuperReal PreMix SYBR Green kit (Tiangen Biotech, China) and the CFX96 Real-Time system (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... Then the PCR products were purified using the Agarose Gel Extraction kit (TIANGEN, Beijing, China), and transformed into Escherichia coli DH5α ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μg of purified RNA was reverse-transcribed in a 20 µL reaction using a FastQuant RT Super Mix Kit (TIANGEN, catalog no. KR106-02). Real-Time PCR was then performed by using Maxima SYBR Green/ROX kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... PCR screening employed the PCR master mix (Tiangen) with the following thermocycling conditions ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was purified and directly recombined by the EasyGeno recombinant clone kits (Cat:VI201, Tiangen). After conventional transformation and monoclonal screening of positive bacteria ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR products underwent agarose gel electrophoresis and gel purification (Universal DNA purification kit, Tiangen, Beijing, China). For animal experiments ...
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bioRxiv - Bioengineering 2023Quote: ... the PCR products were purified after agarose electrophoresis (using Universal DNA purification kit, TIANGEN, Beijing, China), and their concentrations were determined by the OD 260 value by NanoDrop (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... One step Qrt-PCR reaction was performed using the Tiangen One-step SYBR Green kit (Tiangen, China) in the Applied Biosystems QuantStudio 6 Flex RT-PCR System.
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bioRxiv - Immunology 2022Quote: ... cDNA synthesis was executed with 1 µg of total RNA with BcaBEST RNA PCR Kit (TIANGEN, China) as the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... the products of first round extension PCR were purified by gel extraction kit (TIANGEN Biotech, Beijing, China), of which 5 μL of each sample was used as the template in the second extension PCR ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were gel purified and cloned with the TIAN Quick Midi Purification Kit (Tiangen, Beijing, China) following the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2020Quote: ... The cDNAs were synthesized using the FastKing-RT SuperMix (Tiangen, China) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... The PCR products were purified from agarose gels using a TIANgel Purification Kit (TianGen BioTech Co. Ltd, China), followed by nucleotide sequencing performed by Sangon Biotech (Shanghai ...
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bioRxiv - Genetics 2021Quote: ... PCR products from each sample were separately pooled and purified with a Universal DNA Purification Kit (Tiangen, China). In the second-round PCR ...
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Engineering synthetic CopT/A-based genetic circuits for miRNA imaging and functional gene regulationbioRxiv - Synthetic Biology 2023Quote: ... The PCR was performed in triplicate using PCR Mix (TianGen, Beijing) with Fluc primers at 94 °C for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... and dH2O in a 25 μl volume using a QuantiTect SYBR Green PCR kit(TIANGEN BioTech Co.LTD, Beijing, China).Transcripts were quantified with β-actin as an internal standard ...
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bioRxiv - Plant Biology 2019Quote: ... using the Super Real PreMix Plus SYBR Green reagent (TIANGEN Biotech (Beijing) Co.) ...
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bioRxiv - Genetics 2019Quote: The single-stranded viral DNA was isolated from M13mp18 and its 3kb linear dsDNA was amplified by PCR and purified by DNA purification kit (Tiangen). A 60-nt oligomer from M13 genome ...
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bioRxiv - Genetics 2022Quote: ... PCR reactions were performed by using 2×Taq PCR Master Mix (TIANGEN, China). KASP marker analysis was performed on the StepOnePlus Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... A 412 bp TYLCV fragment was PCR amplified using Taq PCR MasterMix (Tiangen, KT201) and primers V61 and C473 as previously described (Atzmon et al. ...
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bioRxiv - Microbiology 2023Quote: ... The cDNA was synthesized for PCR reaction using a PCR master mix (Tiangen, Beijing, China). The specific amplicons were cloned into pMD18T vectors (TaKaRa ...
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bioRxiv - Bioengineering 2019Quote: ... PCR products underwent agarose electrophoresis and gel-purification to remove plasmid template and free dNTPs (Universal DNA purification kit, Tiangen, Beijing, China). The amount of PCR products was determined by the OD 260 absorption value ...
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bioRxiv - Microbiology 2019Quote: ... PCR mixture contained 12 μL of 2× Taq PCR Mix (TIANGEN Co. Ltd., Beijing, PR China), 1 μL DNA template ...
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bioRxiv - Evolutionary Biology 2023Quote: ... directly pipetted into a 25-μl PCR reaction containing a 2x concentration of PCR MasterMix (Tiangen) and 10 pmol of each PCR primer ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The PCR products were purified by excision of the band from agarose gel using the Universal DNA Purification Kit (TIANGEN Bio, Beijing, China), and ligated into a pGEM®-T vector (Promega Co. ...