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Citations for TianGen Biotech :
101 - 117 of 117 citations for hsa mir 222 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... PCR products were gel purified and cloned with the TIAN Quick Midi Purification Kit (Tiangen, Beijing, China) following the manufacturer’s recommendations ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was performed SuperReal PreMix Plus kit (Tiangen Biotech, China) by ABI 7500 Q-PCR system (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... The PCR products were purified from agarose gels using a TIANgel Purification Kit (TianGen BioTech Co. Ltd, China), followed by nucleotide sequencing performed by Sangon Biotech (Shanghai ...
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bioRxiv - Plant Biology 2020Quote: ... The PCR products were directly sent for Sanger sequencing or were further cloned into the pLB vector (TIANGEN). A number of 20 amplicons clones from each plants were analyzed by Sanger sequencing ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR products were inserted into the multiple cloning site of the pLB vector (Tiangen Biotech, Beijing, China) and sent for sequencing ...
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bioRxiv - Genetics 2021Quote: ... PCR products from each sample were separately pooled and purified with a Universal DNA Purification Kit (Tiangen, China). In the second-round PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... and dH2O in a 25 μl volume using a QuantiTect SYBR Green PCR kit(TIANGEN BioTech Co.LTD, Beijing, China).Transcripts were quantified with β-actin as an internal standard ...
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bioRxiv - Genetics 2021Quote: ... PCR amplification was performed with a reaction volume of 25 µl containing 2.5 µl buffer 109 (Tiangen, Beijing, China), 200 µM each dNTP ...
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bioRxiv - Developmental Biology 2019Quote: ... Real-time PCR reactions were carried out with a Real Master Mix SYBR Green Kit (Tiangen, Corp, Beijing, China) using a Stratagene Mx300p (Agilent Technologies Inc ...
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bioRxiv - Genetics 2019Quote: The single-stranded viral DNA was isolated from M13mp18 and its 3kb linear dsDNA was amplified by PCR and purified by DNA purification kit (Tiangen). A 60-nt oligomer from M13 genome ...
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bioRxiv - Molecular Biology 2019Quote: ... we used a miRcute miRNA first-strand cDNA synthesis kit and a miRcute miRNA qRT-PCR detection kit (SYBR Green) (TIANGEN). The miRNA-specific forward primers used for qRT-PCR are also shown in Table S2.
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bioRxiv - Plant Biology 2019Quote: SSR-PCR amplification reactions were conducted using a total volume of 10 µl consisting of 5µl of 2×Taq PCR MasterMix (Tiangen, Beijing, China) (0.1 U/µl Taq DNA Polymerase ...
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bioRxiv - Cell Biology 2020Quote: ... Fluorescence quantitative PCR analyses of all samples were performed using a Bio-Rad CFX96 and SYBR green master mix (TIANGEN, FP215). Semi-quantitative PCR reactions were performed for 30 cycles by 2×TSINGKE Master Mix (TSINGKE ...
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bioRxiv - Plant Biology 2021Quote: ... qRT-PCR analysis of the cDNAs was then conducted using Fast Fire qPCR PreMix (SYBR Green, Tiangen Biotech Company, Beijing, China) on a CFX Connect Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... Reverse transcription and quantitative real-time PCR assay were performed in a Bio-Rad iQ5 real-time system using a Quant One Step qRT-PCR (SYBR Green I) Kit (Tiangen Biotech). Transcript expression levels of FmASP were normalized to that of the reference gene ACTIN and calculated using the 2-ΔΔCt method (Livak and Schmittgen ...
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bioRxiv - Bioengineering 2019Quote: ... PCR products underwent agarose electrophoresis and gel-purification to remove plasmid template and free dNTPs (Universal DNA purification kit, Tiangen, Beijing, China). The amount of PCR products was determined by the OD 260 absorption value ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The PCR products were purified by excision of the band from agarose gel using the Universal DNA Purification Kit (TIANGEN Bio, Beijing, China), and ligated into a pGEM®-T vector (Promega Co. ...