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Citations for TianGen Biotech :
1 - 50 of 89 citations for Zika Virus Vero Cell Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription with ZIKA virus specific primer (Rev-AAGTGATCCATGTGATCAGTTGATCC) was performed using FastQuant RT Kit (Tiangen). Real-time PCR was done on 7900HT (ABI ...
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bioRxiv - Microbiology 2020Quote: The DNA and RNA of virus were extracted by using TIANamp Virus DNA / RNA Kit (TIANGEN) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2023Quote: CT DNA was extracted from the culture supernatant or cell lysates using the TIANamp Genomic DNA kit (Tiangen Biotech, Beijing, China; DP304). Quantitative PCR analysis targeting the CT cryptic plasmid was performed using TaqMan Gene Expression Master (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... And the PRV genome within 200 μL suspension was extracted by TIANamp Virus DNA/RNA Kit (TIANGEN). All cDNAs and viral genome were -20°C.
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bioRxiv - Immunology 2024Quote: RNA from both host and virus was extracted by using the RNAsimple Total RNA Kit (DP419, TIANGEN) and Virus DNA/RNA Extraction Kit (RM401-04 ...
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bioRxiv - Neuroscience 2021Quote: RNA was extracted from mouse brain lysates (mixture of 3∼4 mouse brains for each sample) using RNAprep pure Tissue Kit (Tiangen, DP431). cDNA was synthesized using HiScript II 1st Strand cDNA Synthesis Kit (Vazyme ...
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bioRxiv - Microbiology 2019Quote: ... coli DH5α competent cells (Tiangen). Four clones from each VP1 amplicon were picked for Sanger sequencing ...
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bioRxiv - Bioengineering 2023Quote: ... coli DH5α competent cells (Tiangen). Recombinant plasmids were prepared by using the QIAprep Spin Miniprep Kit (QIAGEN ...
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bioRxiv - Cancer Biology 2024Quote: Whole cell genomic DNA was extracted from LOVO cells using a genomic DNA kit (Tiangen, DP304) and sequences from 2 kb upstream to 100 bp downstream of the gene start codon were amplified using a DNA polymerase kit ...
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bioRxiv - Bioengineering 2023Quote: ... coli BL21(DE3) competent cell (Tiangen) for protein expression.
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bioRxiv - Immunology 2022Quote: ... cell pellets were re-suspended in 5 mL of red blood cell lysis buffer (RT122-02, TIANGEN, China) for 5 min at RT for remove the red blood cell ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cells were collected and lysed by TRIZOL(Tiangen) to extract RNA ...
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bioRxiv - Synthetic Biology 2020Quote: ... mRNA was extracted from the harvested cells according to the manufacturer’s instructions of a RNAprep Pure Cell/Bacteria Kit (Tiangen, Beijing, China). PrimeScript RT reagent Kit with gDNA Eraser (TaKaRa ...
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated from 1×106 THP1 cells after various treatments (see above) by using an RNAprep pure cell/bacteria kit (Tiangen Biotech 4992235) according to the manufacturer’s instructions and was quantified using NanoDrop OneC Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was isolated from cells with Trizol reagent (Tiangen); 1 μg of each RNA sample was used for first-strand cDNA synthesis with oligo (dT)18 and M-MLV reverse transcriptase (Vazyme ...
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bioRxiv - Genomics 2019Quote: ... cells were collected and subjected to RNA isolation (TIANGEN, DP430). Then ...
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bioRxiv - Genetics 2022Quote: Cells were directly lysed into RLT plus buffer (TIANGEN, DP430) for total RNA extraction according to the manufacturer’s instructions provided in the RNeasy Plus Mini Kit ...
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bioRxiv - Immunology 2019Quote: ... The ligation product was transformed into DH5α competent cell (TIANGEN, # CB101), plated on LB solid plates containing kanamycin ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from cells using TRNzol reagent (TIANGEN, China) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... The constructed plasmid was transformed into DH5α cells (CB101, Tiangen, China). Positive clones were selected and sequenced using universal primers (T7 promoter and SP6 terminator primer) ...
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bioRxiv - Microbiology 2022Quote: ... an RNA prep pure cell/bacterial kit (Tiangen Biotech, Beijing, China) was used to prepare total RNA according to the instructions ...
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bioRxiv - Neuroscience 2022Quote: ... The constructed plasmid was transformed into DH5α cells (CB101, Tiangen, China). The positive clones were selected and sequenced using universal primers (T7 promoter and SP6 terminator primer) ...
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bioRxiv - Developmental Biology 2023Quote: Tissue and cells were homogenized with 1 mL TRIZOL (TIANGEN, DP424), after which 200 μL of chloroform was added to the lysates for phase separation ...
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bioRxiv - Bioengineering 2024Quote: ... Total RNA was extracted using RNAprep Pure Cell/Bacteria Kit (TIANGEN) and reverse-transcribed into cDNA using PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time ...
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bioRxiv - Biochemistry 2020Quote: ... Total RNA was extracted using the RNAprep pure Cell/Bacteria Kit (Tiangen) following lysozyme treatment ...
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bioRxiv - Microbiology 2019Quote: ... RNA extraction was performed by RNAprep pure Cell / Bacteria Kit (Tiangen, DP430). The cDNA synthesis was performed by FastKing RT Kit (Tiangen ...
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bioRxiv - Genomics 2019Quote: ... Erythrocytes were cleared by resuspending the cells in ACK lysis buffer (Tiangen) per manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: The total RNA was extracted by the RNAprep pure Cell Kit (TIANGEN), and 1 μg RNA was reversely transcribed into cDNA by using ReverTra Ace® qPCR RT Master Mix with gDNA Remover (TOYOBO) ...
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bioRxiv - Biophysics 2021Quote: ... and HepG2-RC cells were performed by a SuperReal Kit (Tiangen, Beijing, China). The primer sequences are listed in Table S1 ...
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bioRxiv - Biochemistry 2020Quote: Total RNA was extracted from HQ461-treated A549 cells using TRNzol (Tiangen, DP405). Reverse transcription of mRNA into cDNA was performed using the 5X All-In-One RT mastermix kit (Abm,G488 ...
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bioRxiv - Genetics 2021Quote: DNA was extracted from cells using the QIAamp DNA Mini Kit (Tiangen, DP304) and quantified using NanoDrop ...
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bioRxiv - Neuroscience 2022Quote: ... the constructs were transformed into E.coli BL21 (DE3) pLysS competent cells (Tiangen, China). After growth to OD600 = 1 in a SuperBroth medium supplemented with ampicillin ...
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bioRxiv - Cell Biology 2019Quote: ... Red blood cells were lysed using RBC lysis buffer (catalogue NO. RT122-02, TIANGEN). After centrifugation (3,000 rpm ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA from cells were isolated using the RNA simple Total RNA kit (TIANGEN). 1ug RNA was reverse transcribed using a FastKing RT Kit (TIANGEN) ...
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bioRxiv - Physiology 2020Quote: ... RNA was extracted immediately using the RNAprep Pure Cell/Bacteria Kit (Tiangen Biotech (Beijing) CO. ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was extracted using an RNAprep Pure Cell/Bacteria Kit (TIANGEN, Beijing, China; DP430). Two micrograms of RNA were reverse transcribed into cDNA using a PrimeScript™ First-Strand cDNA Synthesis Kit (TaKaRa ...
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bioRxiv - Bioengineering 2022Quote: ... And then cell genomic DNA was isolated using the TIANamp Genomic Kit (Tiangen Biotech) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Total RNA was extracted from cell samples using RNAsimple Total RNA Kit (Tiangen, CHN) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... LwCas13a expression vectors (NovoPro Bioscience; V010159) were transformed into Rosetta (DE3) Competent Cells (Tiangen; CB108). Competent cells containing LwCas13a vectors were inoculated into LB Broth media (Sangon Biotech ...
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bioRxiv - Immunology 2023Quote: Total RNA from the tissues or cultured cells was extracted from TRIzol Universal Reagent (TIANGEN). One microgram of RNA was reverse transcribed into cDNA using HiScript® III RT Super Mix for qPCR (+gDNA wiper ...
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bioRxiv - Cancer Biology 2021Quote: The RM-1 cells of 1×107 WT and Mettl9 KO were extracted with TRNzol (TIANGEN) for total RNA library preparation ...
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bioRxiv - Bioengineering 2021Quote: ... The total RNA was then extracted using an RNAprep Pure Cell/Bacteria Kit (Tiangen Biotech, China). The extracted total RNA was then treated with DNase I to remove the genomic DNA and used as a template for cDNA synthesis with random primers and SuperScriptTMIII Reverse Transcriptase (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Then the crude RNA was purified by RNA prep Pure Cell/Bacteria Kit (TIANGEN, Product #DP430) and genomic DNA was removed by DNase I (NEB ...
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bioRxiv - Cancer Biology 2022Quote: ... and SV40-MES-13 cells was extracted with RNA simple Total RNA Kit (Tiangen, Beijing, China). cDNA was synthesized with TIANScript□RT Kit (Tiangen ...
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bioRxiv - Genetics 2019Quote: ... the genotypes of cell colonies were analyzed by extracting genomic DNA (TIANamp Genomic DNA Kit, TIANGEN) and sequencing ...
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bioRxiv - Cell Biology 2020Quote: gDNA of LV transduced cells was extracted using the TIANamp Genomic DNA Kit (Tiangen, Beijin, China) following the manufacturer’s manual ...
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bioRxiv - Bioengineering 2022Quote: Total RNA was isolated from cultured cells according to the protocols of manufacturers (Tiangen, Beijing, China). cDNA was subsequently synthesized by using a cDNA reverse transcription kit (Toyobo ...
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bioRxiv - Microbiology 2021Quote: Total cellular RNA was extracted using the RNAprep Pure Cell/Bacteria Kit (DP430, Tiangen Biotech Co, Beijing). RNA integrity was examined through agarose gel electrophoresis ...
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bioRxiv - Genomics 2019Quote: ... cells were sorted and collected by FACS assay and were then subjected to RNA isolation (TIANGEN, DP420). Then ...
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bioRxiv - Pathology 2020Quote: ... the genomic DNA from indicated tissues or primary cells were prepared by TIANamp Genomic DNA Kit (TIANGEN) and further identified by PCR using specific primers ...