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Citations for TianGen Biotech :
1 - 50 of 86 citations for Rat Cell Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: PC12 cell or rat liver gDNAs was isolated using the TIANamp blood DNA kit (TIANGEN Biotech, DP304-02) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: CT DNA was extracted from the culture supernatant or cell lysates using the TIANamp Genomic DNA kit (Tiangen Biotech, Beijing, China; DP304). Quantitative PCR analysis targeting the CT cryptic plasmid was performed using TaqMan Gene Expression Master (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: RNA was extracted from mouse brain lysates (mixture of 3∼4 mouse brains for each sample) using RNAprep pure Tissue Kit (Tiangen, DP431). cDNA was synthesized using HiScript II 1st Strand cDNA Synthesis Kit (Vazyme ...
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bioRxiv - Neuroscience 2020Quote: Total RNA was extracted from the rat hippocampus after various treatments using an RNA-simple Total RNA Kit (TIANGEN, China) and then reverse transcribed using a ReverTra Ace qPCR RT Master Mix Kit (TOYOBO ...
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bioRxiv - Microbiology 2019Quote: ... coli DH5α competent cells (Tiangen). Four clones from each VP1 amplicon were picked for Sanger sequencing ...
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bioRxiv - Bioengineering 2023Quote: ... coli DH5α competent cells (Tiangen). Recombinant plasmids were prepared by using the QIAprep Spin Miniprep Kit (QIAGEN ...
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bioRxiv - Cancer Biology 2024Quote: Whole cell genomic DNA was extracted from LOVO cells using a genomic DNA kit (Tiangen, DP304) and sequences from 2 kb upstream to 100 bp downstream of the gene start codon were amplified using a DNA polymerase kit ...
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bioRxiv - Bioengineering 2023Quote: ... coli BL21(DE3) competent cell (Tiangen) for protein expression.
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bioRxiv - Immunology 2022Quote: ... cell pellets were re-suspended in 5 mL of red blood cell lysis buffer (RT122-02, TIANGEN, China) for 5 min at RT for remove the red blood cell ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cells were collected and lysed by TRIZOL(Tiangen) to extract RNA ...
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bioRxiv - Synthetic Biology 2020Quote: ... mRNA was extracted from the harvested cells according to the manufacturer’s instructions of a RNAprep Pure Cell/Bacteria Kit (Tiangen, Beijing, China). PrimeScript RT reagent Kit with gDNA Eraser (TaKaRa ...
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated from 1×106 THP1 cells after various treatments (see above) by using an RNAprep pure cell/bacteria kit (Tiangen Biotech 4992235) according to the manufacturer’s instructions and was quantified using NanoDrop OneC Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was isolated from cells with Trizol reagent (Tiangen); 1 μg of each RNA sample was used for first-strand cDNA synthesis with oligo (dT)18 and M-MLV reverse transcriptase (Vazyme ...
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bioRxiv - Genomics 2019Quote: ... cells were collected and subjected to RNA isolation (TIANGEN, DP430). Then ...
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bioRxiv - Genetics 2022Quote: Cells were directly lysed into RLT plus buffer (TIANGEN, DP430) for total RNA extraction according to the manufacturer’s instructions provided in the RNeasy Plus Mini Kit ...
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bioRxiv - Immunology 2019Quote: ... The ligation product was transformed into DH5α competent cell (TIANGEN, # CB101), plated on LB solid plates containing kanamycin ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from cells using TRNzol reagent (TIANGEN, China) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... The constructed plasmid was transformed into DH5α cells (CB101, Tiangen, China). Positive clones were selected and sequenced using universal primers (T7 promoter and SP6 terminator primer) ...
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bioRxiv - Microbiology 2022Quote: ... an RNA prep pure cell/bacterial kit (Tiangen Biotech, Beijing, China) was used to prepare total RNA according to the instructions ...
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bioRxiv - Neuroscience 2022Quote: ... The constructed plasmid was transformed into DH5α cells (CB101, Tiangen, China). The positive clones were selected and sequenced using universal primers (T7 promoter and SP6 terminator primer) ...
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bioRxiv - Developmental Biology 2023Quote: Tissue and cells were homogenized with 1 mL TRIZOL (TIANGEN, DP424), after which 200 μL of chloroform was added to the lysates for phase separation ...
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bioRxiv - Bioengineering 2024Quote: ... Total RNA was extracted using RNAprep Pure Cell/Bacteria Kit (TIANGEN) and reverse-transcribed into cDNA using PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time ...
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bioRxiv - Biochemistry 2020Quote: ... Total RNA was extracted using the RNAprep pure Cell/Bacteria Kit (Tiangen) following lysozyme treatment ...
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bioRxiv - Microbiology 2019Quote: ... RNA extraction was performed by RNAprep pure Cell / Bacteria Kit (Tiangen, DP430). The cDNA synthesis was performed by FastKing RT Kit (Tiangen ...
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bioRxiv - Genomics 2019Quote: ... Erythrocytes were cleared by resuspending the cells in ACK lysis buffer (Tiangen) per manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: The total RNA was extracted by the RNAprep pure Cell Kit (TIANGEN), and 1 μg RNA was reversely transcribed into cDNA by using ReverTra Ace® qPCR RT Master Mix with gDNA Remover (TOYOBO) ...
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bioRxiv - Biophysics 2021Quote: ... and HepG2-RC cells were performed by a SuperReal Kit (Tiangen, Beijing, China). The primer sequences are listed in Table S1 ...
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bioRxiv - Biochemistry 2020Quote: Total RNA was extracted from HQ461-treated A549 cells using TRNzol (Tiangen, DP405). Reverse transcription of mRNA into cDNA was performed using the 5X All-In-One RT mastermix kit (Abm,G488 ...
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bioRxiv - Genetics 2021Quote: DNA was extracted from cells using the QIAamp DNA Mini Kit (Tiangen, DP304) and quantified using NanoDrop ...
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bioRxiv - Neuroscience 2022Quote: ... the constructs were transformed into E.coli BL21 (DE3) pLysS competent cells (Tiangen, China). After growth to OD600 = 1 in a SuperBroth medium supplemented with ampicillin ...
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bioRxiv - Cell Biology 2019Quote: ... Red blood cells were lysed using RBC lysis buffer (catalogue NO. RT122-02, TIANGEN). After centrifugation (3,000 rpm ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA from cells were isolated using the RNA simple Total RNA kit (TIANGEN). 1ug RNA was reverse transcribed using a FastKing RT Kit (TIANGEN) ...
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bioRxiv - Physiology 2020Quote: ... RNA was extracted immediately using the RNAprep Pure Cell/Bacteria Kit (Tiangen Biotech (Beijing) CO. ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was extracted using an RNAprep Pure Cell/Bacteria Kit (TIANGEN, Beijing, China; DP430). Two micrograms of RNA were reverse transcribed into cDNA using a PrimeScript™ First-Strand cDNA Synthesis Kit (TaKaRa ...
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bioRxiv - Bioengineering 2022Quote: ... And then cell genomic DNA was isolated using the TIANamp Genomic Kit (Tiangen Biotech) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Total RNA was extracted from cell samples using RNAsimple Total RNA Kit (Tiangen, CHN) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... LwCas13a expression vectors (NovoPro Bioscience; V010159) were transformed into Rosetta (DE3) Competent Cells (Tiangen; CB108). Competent cells containing LwCas13a vectors were inoculated into LB Broth media (Sangon Biotech ...
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bioRxiv - Immunology 2023Quote: Total RNA from the tissues or cultured cells was extracted from TRIzol Universal Reagent (TIANGEN). One microgram of RNA was reverse transcribed into cDNA using HiScript® III RT Super Mix for qPCR (+gDNA wiper ...
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bioRxiv - Cancer Biology 2021Quote: The RM-1 cells of 1×107 WT and Mettl9 KO were extracted with TRNzol (TIANGEN) for total RNA library preparation ...
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bioRxiv - Bioengineering 2021Quote: ... The total RNA was then extracted using an RNAprep Pure Cell/Bacteria Kit (Tiangen Biotech, China). The extracted total RNA was then treated with DNase I to remove the genomic DNA and used as a template for cDNA synthesis with random primers and SuperScriptTMIII Reverse Transcriptase (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Then the crude RNA was purified by RNA prep Pure Cell/Bacteria Kit (TIANGEN, Product #DP430) and genomic DNA was removed by DNase I (NEB ...
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bioRxiv - Cancer Biology 2022Quote: ... and SV40-MES-13 cells was extracted with RNA simple Total RNA Kit (Tiangen, Beijing, China). cDNA was synthesized with TIANScript□RT Kit (Tiangen ...
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bioRxiv - Genetics 2019Quote: ... the genotypes of cell colonies were analyzed by extracting genomic DNA (TIANamp Genomic DNA Kit, TIANGEN) and sequencing ...
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bioRxiv - Cell Biology 2020Quote: gDNA of LV transduced cells was extracted using the TIANamp Genomic DNA Kit (Tiangen, Beijin, China) following the manufacturer’s manual ...
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bioRxiv - Bioengineering 2022Quote: Total RNA was isolated from cultured cells according to the protocols of manufacturers (Tiangen, Beijing, China). cDNA was subsequently synthesized by using a cDNA reverse transcription kit (Toyobo ...
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bioRxiv - Microbiology 2021Quote: Total cellular RNA was extracted using the RNAprep Pure Cell/Bacteria Kit (DP430, Tiangen Biotech Co, Beijing). RNA integrity was examined through agarose gel electrophoresis ...
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bioRxiv - Genomics 2019Quote: ... cells were sorted and collected by FACS assay and were then subjected to RNA isolation (TIANGEN, DP420). Then ...
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bioRxiv - Pathology 2020Quote: ... the genomic DNA from indicated tissues or primary cells were prepared by TIANamp Genomic DNA Kit (TIANGEN) and further identified by PCR using specific primers ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from sorted hair cells using the RNAprep Pure Micro Kit (DP420, Tiangen Biotech) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Total RNAs (1 μg) of experimental cells were extracted with the RNA simple kit (Tiangen, Beijing, China) and added to the reverse transcriptase reaction to obtain the first strand of cDNAs by using RevertAid First Strand cDNA Synthesis kit (K1622 ...