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Citations for TianGen Biotech :
451 - 500 of 527 citations for QuantiChrom Hyaluronidase Inhibitor Screening Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Genome DNA from the collected cells of P2 was extracted under the instructions of the user’s manual for TIANamp Genomic DNA Kit (TIANGEN, DP304-02). PCR was later conducted to amplify mitochondrial COI gene fragment using the primer pair as follows ...
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bioRxiv - Evolutionary Biology 2022Quote: Genomic DNA (gDNA) was isolated from adductor muscle tissues in parents and offspring using the TIANamp Marine Animals DNA Kit (TIANGEN, Beijing). Library preparation and high-throughput sequencing were conducted by Novogene (Beijing ...
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bioRxiv - Genetics 2019Quote: ... the template DNA was linearized by XbaI digestion (for Cas9 and qCas9 mRNA) or NotI digestion (for EGFP and EGFP-PEST mRNA) and then purified by TIANquick Mini Purification Kit (TIANGEN Biotech). The capped Cas9/qCas9 and EGFP/EGFP-PEST mRNA were synthesized by mMESSAGE mMACHINE T7 or SP6 kit (Invitrogen) ...
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bioRxiv - Biochemistry 2019Quote: ... The extraction of total RNA from inoculated and non-inoculated control samples was carried-out with RNAprep pure Plant Kit (Tiangen, Beijing) by following processes recommended by the manufacturer ...
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bioRxiv - Synthetic Biology 2020Quote: ... mRNA was extracted from the harvested cells according to the manufacturer’s instructions of a RNAprep Pure Cell/Bacteria Kit (Tiangen, Beijing, China). PrimeScript RT reagent Kit with gDNA Eraser (TaKaRa ...
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bioRxiv - Plant Biology 2020Quote: ... The pCSGAPO1 vector (Cong et al., 2013) was digested with Bsa I and purified using a DNA purification kit (Tiangen, China). A ligation reaction (10 μl ...
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bioRxiv - Plant Biology 2021Quote: ... and the leaf samples were pooled for each group for DNA extraction using the TIANGEN DNA purification kit (TIANGEN, DP305-02). High-throughput sequencing and Bulked Sequence Analysis (BSA ...
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bioRxiv - Neuroscience 2021Quote: RNA was extracted from mouse brain lysates (mixture of 3∼4 mouse brains for each sample) using RNAprep pure Tissue Kit (Tiangen, DP431). cDNA was synthesized using HiScript II 1st Strand cDNA Synthesis Kit (Vazyme ...
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bioRxiv - Cell Biology 2021Quote: ... the first batch of cells (5×106) were collected 2 days after infection using the TIANamp genomic DNA kit (Tiangen, DP304), and the second batch of cells treated with puromycin were collected at 7d ...
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bioRxiv - Genomics 2022Quote: ... on an agarose gel and were subsequently extracted using a Universal DNA purification Kit (Catalog No. DP214-02, TIANGEN, Beijing, China) and then used as a template to synthesize dsRNA with a MEGAscript RNAi Kit (Catalog No ...
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bioRxiv - Plant Biology 2022Quote: ... Reverse transcription and quantitative real-time PCR assay were performed in a Bio-Rad iQ5 real-time system using a Quant One Step qRT-PCR (SYBR Green I) Kit (Tiangen Biotech). Transcript expression levels of FmASP were normalized to that of the reference gene ACTIN and calculated using the 2-ΔΔCt method (Livak and Schmittgen ...
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bioRxiv - Plant Biology 2022Quote: ... the young leaves of eight to ten seedlings were sampled and pooled for genomic DNA extraction using the Plant Genomic DNA Extraction Kit (DP205, TianGen, China) following the instructions provided by the supplier.
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from less than 100 mg of 10-d-old seedlings that were grown vertically on half-strength MS plates or treated with 50 μM AA or an equal volume of DMSO for 4 h in liquid MS medium using RNAprep Pure Plant Plus Kit (TIANGEN, DP441). First-strand cDNA was synthesized using a HiScript® II cDNA Synthesis Kit (Vazyme R212-02 ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from leaf and root tissues of the natural population and transgenic rapeseed using RNAsimple Kit (DP419, TIANGEN, China). The RNA was sequenced using Illumina 2500 platform (Illumina Inc ...
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bioRxiv - Neuroscience 2022Quote: ... The strains were expanded and cultured for endotoxin-free plasmid extraction with the EndoFree Maxi Plasmid Kit V2 (DP120, Tiangen, China). The constructed plasmids were stored at −20 °C for the transfection of HEK293-T cells (49).
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bioRxiv - Microbiology 2022Quote: The DNA of pure bacterial colonies was extracted by following the procedures described in the DNA extraction kit (TIANGEN Biotech, Beijing). The 16S rRNA gene was amplified using universal primers 27F (5’-AGAGTTTGATCMTGGCTCAG-3’ ...
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bioRxiv - Cancer Biology 2023Quote: The total RNAs were extracted from experimental cells when they reached 80% confluency using an RNA extraction kit (TIANGEN, Beijing, China) and then reversely transcribed into single-stranded cDNAs by a Reverse-Transcription Kit (Promega ...
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bioRxiv - Immunology 2023Quote: ... hCD36 mice below refer to homozygous hCD36 mice) and wild-type (WT) C57BL/6J were harvested for extracting total RNA using RNAprep Pure Cell / Bacteria Kit (TIANGEN, DP430). The mRNA expression of hCD36 and mCD36 was determined using GAPDH as the internal control ...
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bioRxiv - Physiology 2023Quote: Total RNA was extracted from middle part of ear leaf at 12 DAI with the RNA extraction Kit (DP411, TIANGEN, China). RNA purity ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from cultured cells by using TRIZOL reagent and 500 ng to 2 μg RNA was reversely transcribed into first-strand cDNA by FastKing RT Kit (Tiangen, KR116). qPCR analysis was performed with Mastercycler Realplex2 (Eppendorf ...
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bioRxiv - Genetics 2024Quote: ... First-strand cDNA was synthesized from 2 μg of DNase I-treated total RNA using the FastKing RT kit (TIANGEN, KR116). qRT-PCR was performed using the ChamQ Universal SYBR qPCR Master Mix (Vazyme ...
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bioRxiv - Genomics 2024Quote: Total RNA was extracted from blood using TRIzol reagent in an RNAprep Pure Tissue Kit (Cat# 4992236, TIANGEN Biotech, Beijing, China). After the RNA purity and concentration were determined with Nanodrop (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... A paired-end library with an insert size of 350 kb was constructed using the TIANSeq Fast DNA Library Kit (Cat. no: 4992261; Tiangen, China) and then sequenced using an Illumina NovaSeq6000 sequenator (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... viral RNA was extracted from mock- or ALSV-infected cell-culture supernatants using a viral RNA extraction kit (TIANGEN, Cat# DP315), and transcribed into cDNA using the cDNA Synthesis SuperMix (TransGen ...
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bioRxiv - Immunology 2024Quote: ... The correct construction of the recombinant plasmids was verified through Sanger sequencing and extracted using an Endotoxin-Free Plasmid DNA Miniprep Kit (Tiangen, China) before using plasmids ...
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bioRxiv - Plant Biology 2024Quote: Total RNAs was extracted from 100 mg cotton seedling samples with different treatments by grinding with Plant Total RNA Purification Kit (TIANGEN, China). RNA was then treated with DNase using the DNA-free kit following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: Total RNA samples extracted from M82 and yft1 pericarp (47 dpa, BR stage) using the RNAprep pure Plant Kit (Tiangen, Beijing, China) were used as a templates in rapid amplification of cDNA ends (RACE ...
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bioRxiv - Plant Biology 2020Quote: ... AAA-CAA) within the PGEX-6P-1 plasmid was performed using the fast Site-Directed Mutagenesis Kit (Tiangen, China, Catalog No. KM101). PCR was conducted using site-specific primers (Supplemental Table S17) ...
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bioRxiv - Microbiology 2021Quote: ... of strain TC967 was extracted from 4-day-old mycelia following the protocol described in the handbook of the Plant Genomic DNA Kit (Tiangen, Beijing, China). The gDNA was sequenced by Illumina HisSeq 2500 sequencing (Illumina ...
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bioRxiv - Genetics 2022Quote: ... First-strand cDNA synthesis from the total RNA was performed by using the FastKing RT kit (with gDNase) (TianGen Biotech Co, Beijing). The expression analyses were performed using the primer set (F-GGACTTCCCTTGGATCCTGC and R-ACCGACAATCATGTCCGCAT) ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was isolated from single third-instar larva of three strains (total 30 larvae of each strain) using the DNA/RNA/Protein Isolation Kit (TianGen, Beijing, China). The first-strand cDNA was synthesized with 1 μg of total RNA using a PrimeScript™ 1st Strand cDNA Synthesis Kit with gDNA Eraser (TaKaRa Biotechnology ...
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bioRxiv - Genetics 2019Quote: ... and the whole genome DNA of the patients was extracted using DNA extraction kit (product of Beijing Tiangen Biochemical Technology Co., Ltd.) and using BloodGen Midi Kit (CWBIO ...
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bioRxiv - Synthetic Biology 2021Quote: ... The incubated solution is then subjected to agarose gel electrophoresis and the correct band is extracted using a TIANgeo Midi Purification Kit (TIANGEN, DP209-02) or AxyPrep DNA gel Extraction Kit (AXYGEN ...
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bioRxiv - Synthetic Biology 2021Quote: ... The incubated solution is then subjected to agarose gel electrophoresis and the correct band is extracted using a TIANgeo Midi Purification Kit (TIANGEN, DP209-02) or AxyPrep DNA gel Extraction Kit (AXYGEN ...
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bioRxiv - Plant Biology 2020Quote: RNA was extracted according to the instructions of the RNA Isolation Kit for polyphenol- and polysaccharide-rich plants (Tiangen Biotech, Beijing, China). The reverse transcription primer was Oligo(dT)18 ...
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bioRxiv - Plant Biology 2020Quote: ... MG), 47 dpa(Breaker stage, BR) and 54 dpa(Red Ripe stage, RR) using the RNAprep pure Plant Kit (Tiangen, Beijing, China) based on the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA (1 µg) was used as a template for cDNA synthesis with a QuantScript RT Kit (KR103; Tiangen Biotech, Beijing, China). To determine mRNA expression levels ...
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bioRxiv - Bioengineering 2019Quote: ... PCR products underwent agarose electrophoresis and gel-purification to remove plasmid template and free dNTPs (Universal DNA purification kit, Tiangen, Beijing, China). The amount of PCR products was determined by the OD 260 absorption value ...
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bioRxiv - Bioengineering 2019Quote: ... The cell suspension was centrifuged for 5 minutes at 12,000 rpm and cell precipitate was resuspend in 1 ml sterilizing saline used for DNA extraction with TIANamp Genomic DNA kit (TianGen Biotechnologies, Beijing). Subsequently ...
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bioRxiv - Evolutionary Biology 2019Quote: ... DNA was extracted from the 2nd pair or 3rd pair of leaf counted from the top following the protocol of TIANquick Midi Purificatioin kit (TIANGEN Biotech, Beijing). The primers were listed in Table S2.
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bioRxiv - Plant Biology 2021Quote: ... Genomic DNA has been extracted from putative transformants one-month-old leaves grown on a kanamycin-containing medium using TianGen kits (TianGen BioTech, China). The quality of the extracted genomic DNA (250–350 ng/μl ...
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bioRxiv - Genetics 2021Quote: Genomic DNA was extracted from peripheral blood samples collected from all the participants using the blood DNA extraction kit (TianGen Biotech, China). We performed the whole genome sequencing on Illumina NovaSeq 6000 system (150 bp paired-end reads ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA was extracted from blood samples collected from the wing vein using a DNA extraction mini kit (Tiangen Biotech, Beijing, China). All sampled hens were killed by cervical dislocation immediately after oviposition and the abdominal cavity was opened ...
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bioRxiv - Plant Biology 2022Quote: ... and total mRNA was extracted using the RNA Prep Pure Plant Plus Kit according to the manufacturer’s instructions (Tiangen Biotech, Beijing, China). A strand-switching method and the cDNA-PCR Sequencing Kit (SQK-PCS109 ...
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bioRxiv - Bioengineering 2023Quote: Total RNAs were isolated from all samples with liquid nitrogen using the RNAprep Plant RNA kit (polysaccharides&polyphenolics-rich) (TIANGEN BIOTECH (BEIJING)CO. ...
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bioRxiv - Cell Biology 2023Quote: Experimental cells were subjected to total RNA isolation (using an RNA Simple Total RNA Kit, DP419, Tiangen Biotech Co., Ltd., Beijing, China). The first strand of cDNA was obtained by adding total RNA (1.5 µg ...
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated from 1×106 THP1 cells after various treatments (see above) by using an RNAprep pure cell/bacteria kit (Tiangen Biotech 4992235) according to the manufacturer’s instructions and was quantified using NanoDrop OneC Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: CT DNA was extracted from the culture supernatant or cell lysates using the TIANamp Genomic DNA kit (Tiangen Biotech, Beijing, China; DP304). Quantitative PCR analysis targeting the CT cryptic plasmid was performed using TaqMan Gene Expression Master (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... the DNA fragments were separated by 1.0 % agarose gel electrophoresis (1 × TAE buffer) and purified from the gel with the DNA Gel Extraction Kit (TianGen Biotech., Beijing, China), then they were sub-cloned into pCE2 TA/Blunt-Zero vector (Vazyme ...
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bioRxiv - Molecular Biology 2024Quote: Genomic DNA was isolated from the cecal contents using the magnetic-based soil and stool DNA extraction kit (TIANGEN®, Beijing, China). The concentration of the extracted DNA was determined using the Qubit Fluorometric Quantitation system (Thermo Fisher Scientific ...