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Citations for TianGen Biotech :
51 - 100 of 103 citations for Peripheral Blood Mononuclear Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... And then cell genomic DNA was isolated using the TIANamp Genomic Kit (Tiangen Biotech) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Total RNA was extracted from cell samples using RNAsimple Total RNA Kit (Tiangen, CHN) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... LwCas13a expression vectors (NovoPro Bioscience; V010159) were transformed into Rosetta (DE3) Competent Cells (Tiangen; CB108). Competent cells containing LwCas13a vectors were inoculated into LB Broth media (Sangon Biotech ...
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bioRxiv - Immunology 2023Quote: Total RNA from the tissues or cultured cells was extracted from TRIzol Universal Reagent (TIANGEN). One microgram of RNA was reverse transcribed into cDNA using HiScript® III RT Super Mix for qPCR (+gDNA wiper ...
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bioRxiv - Cancer Biology 2021Quote: The RM-1 cells of 1×107 WT and Mettl9 KO were extracted with TRNzol (TIANGEN) for total RNA library preparation ...
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bioRxiv - Bioengineering 2021Quote: ... The total RNA was then extracted using an RNAprep Pure Cell/Bacteria Kit (Tiangen Biotech, China). The extracted total RNA was then treated with DNase I to remove the genomic DNA and used as a template for cDNA synthesis with random primers and SuperScriptTMIII Reverse Transcriptase (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Then the crude RNA was purified by RNA prep Pure Cell/Bacteria Kit (TIANGEN, Product #DP430) and genomic DNA was removed by DNase I (NEB ...
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bioRxiv - Cancer Biology 2022Quote: ... and SV40-MES-13 cells was extracted with RNA simple Total RNA Kit (Tiangen, Beijing, China). cDNA was synthesized with TIANScript□RT Kit (Tiangen ...
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bioRxiv - Genetics 2019Quote: ... the genotypes of cell colonies were analyzed by extracting genomic DNA (TIANamp Genomic DNA Kit, TIANGEN) and sequencing ...
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bioRxiv - Cell Biology 2020Quote: gDNA of LV transduced cells was extracted using the TIANamp Genomic DNA Kit (Tiangen, Beijin, China) following the manufacturer’s manual ...
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bioRxiv - Bioengineering 2022Quote: Total RNA was isolated from cultured cells according to the protocols of manufacturers (Tiangen, Beijing, China). cDNA was subsequently synthesized by using a cDNA reverse transcription kit (Toyobo ...
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bioRxiv - Microbiology 2021Quote: Total cellular RNA was extracted using the RNAprep Pure Cell/Bacteria Kit (DP430, Tiangen Biotech Co, Beijing). RNA integrity was examined through agarose gel electrophoresis ...
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bioRxiv - Genomics 2019Quote: ... cells were sorted and collected by FACS assay and were then subjected to RNA isolation (TIANGEN, DP420). Then ...
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bioRxiv - Pathology 2020Quote: ... the genomic DNA from indicated tissues or primary cells were prepared by TIANamp Genomic DNA Kit (TIANGEN) and further identified by PCR using specific primers ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from sorted hair cells using the RNAprep Pure Micro Kit (DP420, Tiangen Biotech) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Total RNAs (1 μg) of experimental cells were extracted with the RNA simple kit (Tiangen, Beijing, China) and added to the reverse transcriptase reaction to obtain the first strand of cDNAs by using RevertAid First Strand cDNA Synthesis kit (K1622 ...
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bioRxiv - Cancer Biology 2023Quote: ... Human genome DNA extracted from HCT116 cells using the TIANamp Genomic DNA Kit (Tiangen Biotech, Beijing, China) was used as template for amplifying the promoter regions ...
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bioRxiv - Molecular Biology 2020Quote: Experimental cells were subjected to isolation of total RNAs by using the RNAsimple Kit (DP419, Tiangen Biotech, China). Then ...
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bioRxiv - Immunology 2019Quote: Plasmids of the four constructed expression vectors were extracted and transformed into Rosetta (DE3) competent cell (TIANGEN, # CB108), respectively ...
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bioRxiv - Microbiology 2020Quote: ... Δ N1-SpyCatcher-I53-50A1.1PT1 and I53-50B.4PT1 was transformed into Rosetta™ (DE3) competent cells (TIANGEN). After incubation for overnight at 37 °C on TB-agars culture plate supplemented with 50 mg/mL kanamycin and 33 mg/mL chloramphenicol ...
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bioRxiv - Cancer Biology 2019Quote: Experimental cells were subjected to the extraction of total RNAs by using an RNAsimple kit (Tiangen, Beijing, China). Then ...
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bioRxiv - Genetics 2022Quote: ... the cells were collected with sufficient material for DNA isolation with a TIANamp Genomic DNA Kit (DP304, TIANGEN) for genotyping analysis in technical triplicate for genotyping analysis ...
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bioRxiv - Molecular Biology 2023Quote: ... the DNA of successfully transfected cells or AAV8 treatment tissues were extracted with TIANamp Genomic DNA Kit(TIANGEN,) according to the manufacturer protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was extracted from each cell culture flask with Plant genomic DNA kits (Tiangen BioTech., Beijing, China). Genomic PCR (gPCR ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from the treated microbial cells with a microbial genomic DNA extraction kit (Tiangen Biotech, Beijing, China) following the manufacturer’s protocol.
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bioRxiv - Genetics 2019Quote: The genomic DNA was isolated from the cells using Tiangen genomic DNA extraction Kit (Catalog#DP304, TianGen Biotech, China) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... total RNA was extracted from bat intestine tissue using the RNAprep pure Kit (for Cell/Bacteria) (TIANGEN, Beijing, China). First-strand complementary DNA was synthesized from total RNA by reverse transcription with random hexamers ...
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bioRxiv - Molecular Biology 2021Quote: ... total cellular RNA was extracted from CSFV-infecting PK-15 cells or positive clones using TRNzol Universal Reagent (TIANGEN) and ∼2 μg RNAs were performed to reverse transcript to the first-strand cDNAs using FastKing RT Kit (TINAGEN ...
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bioRxiv - Genetics 2023Quote: Genomic DNA was isolated from the iPS cell line using the Magetic Univeral Genomic DNA kit (TIANGEN, DP705-01). Copy numbers of ampliconic genes were validated through droplet digital PCR (ddPCR) ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA extraction from each sample employed the RNA Easy Fast Cell Total RNA Extraction Kit (DP451, Tiangen, Beijing), following the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from mouse testes using an RNA Easy Fast Tissue/Cell Kit (Tiangen Biotech, China, 4992732). Approximately 0.3 mg of total RNA was converted into cDNA with the PrimeScript RT Reagent Kit (Takara ...
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bioRxiv - Molecular Biology 2020Quote: Experimental cells were subjected to isolation of total RNAs by using the RNA simple Kit (Tiangen Biotech Co., Beijing, China). Then ...
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bioRxiv - Cancer Biology 2019Quote: ... and HL7702 cell lines after transfection with pTsgRNA-DMP-Cas9-EGFP 24 h using the TIANamp Genomic DNA Kit (Tiangen) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... the GFP positive cells were sorted out by MoFlo XDP machine and the genomic DNA was isolated using TIANamp Genomic DNA Kit (TIANGEN) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: Total RNAs were prepared from cells using the RNA Simple Total RNA Kit (DP419, Tiangen Biotech Co., Ltd., Beijing, China), and reverse transcription was performed using the Revert Aid First Strand cDNA Synthesis Kit from Thermo ...
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bioRxiv - Cancer Biology 2023Quote: ... total RNA from the cells plated on 96 well plates were first extracted and purified using the RNAprep Pure Micro Kit (TIANGEN). Reverse transcription was performed with HiScript III cDNA Synthesis Kit (Vazyme) ...
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bioRxiv - Genetics 2023Quote: ... and plasmids were assembled by using HiFi DNA Assembly Cloning Kit and subsequently transformed into DH5α competent cells from TIANGEN. ZymoPure Midiprep Kit (Zymo Research ...
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bioRxiv - Cell Biology 2020Quote: Genome DNA from the collected cells of P2 was extracted under the instructions of the user’s manual for TIANamp Genomic DNA Kit (TIANGEN, DP304-02). PCR was later conducted to amplify mitochondrial COI gene fragment using the primer pair as follows ...
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bioRxiv - Biochemistry 2020Quote: ... Ethanol were then removed and cells were resuspended using PBS supplemented with 40 μg/mL propidium iodide (PI, Leagene, China) and RNaseA (TIANGEN, China). Cell cycle analysis was performed on a BD Calibur Cytometer (Becton Dickinson ...
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bioRxiv - Cell Biology 2021Quote: ... the first batch of cells (5×106) were collected 2 days after infection using the TIANamp genomic DNA kit (Tiangen, DP304), and the second batch of cells treated with puromycin were collected at 7d ...
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bioRxiv - Cancer Biology 2023Quote: The total RNAs were extracted from experimental cells when they reached 80% confluency using an RNA extraction kit (TIANGEN, Beijing, China) and then reversely transcribed into single-stranded cDNAs by a Reverse-Transcription Kit (Promega ...
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bioRxiv - Immunology 2023Quote: ... hCD36 mice below refer to homozygous hCD36 mice) and wild-type (WT) C57BL/6J were harvested for extracting total RNA using RNAprep Pure Cell / Bacteria Kit (TIANGEN, DP430). The mRNA expression of hCD36 and mCD36 was determined using GAPDH as the internal control ...
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bioRxiv - Microbiology 2024Quote: ... viral RNA was extracted from mock- or ALSV-infected cell-culture supernatants using a viral RNA extraction kit (TIANGEN, Cat# DP315), and transcribed into cDNA using the cDNA Synthesis SuperMix (TransGen ...
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bioRxiv - Bioengineering 2019Quote: ... The cell suspension was centrifuged for 5 minutes at 12,000 rpm and cell precipitate was resuspend in 1 ml sterilizing saline used for DNA extraction with TIANamp Genomic DNA kit (TianGen Biotechnologies, Beijing). Subsequently ...
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bioRxiv - Zoology 2020Quote: ... The recombinant plasmids pET-30a-RhATG5 and pET-30a-RhATG5191-199Δ were constructed and transformed into BL21 (DE3) competent Escherichia coli cells (Tiangen, Beijing, China). To induce the expression of the recombinant proteins RhATG5 and RhATG5191-199Δ ...
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bioRxiv - Cell Biology 2023Quote: Experimental cells were subjected to total RNA isolation (using an RNA Simple Total RNA Kit, DP419, Tiangen Biotech Co., Ltd., Beijing, China). The first strand of cDNA was obtained by adding total RNA (1.5 µg ...
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bioRxiv - Microbiology 2023Quote: CT DNA was extracted from the culture supernatant or cell lysates using the TIANamp Genomic DNA kit (Tiangen Biotech, Beijing, China; DP304). Quantitative PCR analysis targeting the CT cryptic plasmid was performed using TaqMan Gene Expression Master (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: Each of experimental cell lines was subject to its total RNAs isolated by employing an RNAsimple Kit (Tiangen Biotech CO. LTD, Beijing, China). Total RNAs (1 μg ...
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bioRxiv - Physiology 2021Quote: ... Cells harboring correct plasmid were cultured and pL4440-vit2 was extracted with the TIANprep Mini Plasmid Kit (Tiangen Biotech Co. Ltd., Beijing, China). Meanwhile ...
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bioRxiv - Microbiology 2022Quote: ... at which point 1 mL of cells were harvested and RNA samples were extracted using the RNAprep Pure Bacteria Kit (Tiangen Co., Beijing, China). Total cDNA was generated from 500 ng of total template RNA using the HiScript III All-in-one RT SuperMix Perfect for qPCR (Vazyme Co. ...