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Citations for TianGen Biotech :
1 - 50 of 578 citations for Onion Yellow Dwarf Virus OYDV PCR Kits since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: The DNA and RNA of virus were extracted by using TIANamp Virus DNA / RNA Kit (TIANGEN) according to the manufacturer’s protocols ...
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bioRxiv - Bioengineering 2024Quote: ... according to TIANamp Virus DNA/RNA Kit (TIANGEN, DP315).
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription with ZIKA virus specific primer (Rev-AAGTGATCCATGTGATCAGTTGATCC) was performed using FastQuant RT Kit (Tiangen). Real-time PCR was done on 7900HT (ABI ...
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bioRxiv - Molecular Biology 2021Quote: ... And the PRV genome within 200 μL suspension was extracted by TIANamp Virus DNA/RNA Kit (TIANGEN). All cDNAs and viral genome were -20°C.
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bioRxiv - Immunology 2024Quote: RNA from both host and virus was extracted by using the RNAsimple Total RNA Kit (DP419, TIANGEN) and Virus DNA/RNA Extraction Kit (RM401-04 ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were purified using a PCR purification kit (Tiangen, Beijing, China) and sequenced by Sangon Biotech Co. ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR products were purified by TIAN quick Midi Purification Kit (TIANGEN) and genetic circuits were constructed with standard vector parts by employing the Golden Gate Assembly[4] ...
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bioRxiv - Immunology 2020Quote: ... PCR products underwent agarose electrophoresis and gel-purification (Universal DNA purification kit, Tiangen). The amount of PCR products was determined by OD 260 absorption value ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were merged and purified by using TIANquick Midi Purification Kit (TIANGEN). The NGS libraries were prepared by using VAHTS Universal DNA Library Prep Kit for Illumina (Vazyme ...
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bioRxiv - Immunology 2022Quote: SARS-CoV-2 RBD-specific quantitative reverse transcription-PCR (qRT-PCR) assays were performed by using a FastKing One Step Probe RT-qPCR kit (Tiangen Biotech, China) on a CFX96 Touch real-time PCR detection system (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: SARS-CoV-2 RBD-specific quantitative reverse transcription-PCR (qRT-PCR) assays were performed by using a FastKing One Step Probe RT-qPCR kit (Tiangen Biotech, China) on a CFX96 Touch real-time PCR detection system (Bio-Rad ...
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bioRxiv - Plant Biology 2021Quote: ... The qRT-PCR was completed with the FastKing RT Kit (with gDNase) (TIANGEN, Beijing) and the QuantStudio™ 7 Flex Real-Time PCR System (Life Technologies Corporation ...
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bioRxiv - Plant Biology 2020Quote: ... The qRT-PCR was completed with the FastKing RT Kit (with gDNase) (TIANGEN, Beijing) and the QuantStudio™ 7 Flex Real-Time PCR System (Life Technologies Corporation ...
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bioRxiv - Cancer Biology 2023Quote: ... The PCR products were extracted by a gel extraction kit (TIANGEN, Catalog # DP209-03). Data analysis was performed using MAGeCK.
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bioRxiv - Bioengineering 2021Quote: ... The quantitative PCR was conducted using the SuperReal PreMix SYBR Green kit (Tiangen Biotech, China) and the CFX96 Real-Time system (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was purified and directly recombined by the EasyGeno recombinant clone kits (Cat:VI201, Tiangen). After conventional transformation and monoclonal screening of positive bacteria ...
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bioRxiv - Bioengineering 2023Quote: ... the PCR products were purified after agarose electrophoresis (using Universal DNA purification kit, TIANGEN, Beijing, China), and their concentrations were determined by the OD 260 value by NanoDrop (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... One step Qrt-PCR reaction was performed using the Tiangen One-step SYBR Green kit (Tiangen, China) in the Applied Biosystems QuantStudio 6 Flex RT-PCR System.
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bioRxiv - Immunology 2022Quote: ... cDNA synthesis was executed with 1 µg of total RNA with BcaBEST RNA PCR Kit (TIANGEN, China) as the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Follow the reverse transcription box instructions for reverse transcription (FastKing One Step RT-PCR Kit, KR123, Tiangen). Real-time PCR (Talent qPCR PreMix (SYBR Green) ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were gel purified and cloned with the TIAN Quick Midi Purification Kit (Tiangen, Beijing, China) following the manufacturer’s recommendations ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was performed SuperReal PreMix Plus kit (Tiangen Biotech, China) by ABI 7500 Q-PCR system (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... The PCR products were purified from agarose gels using a TIANgel Purification Kit (TianGen BioTech Co. Ltd, China), followed by nucleotide sequencing performed by Sangon Biotech (Shanghai ...
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bioRxiv - Genetics 2021Quote: ... PCR products from each sample were separately pooled and purified with a Universal DNA Purification Kit (Tiangen, China). In the second-round PCR ...
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Engineering synthetic CopT/A-based genetic circuits for miRNA imaging and functional gene regulationbioRxiv - Synthetic Biology 2023Quote: ... The PCR was performed in triplicate using PCR Mix (TianGen, Beijing) with Fluc primers at 94 °C for 5 min ...
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bioRxiv - Bioengineering 2024Quote: ... RT-PCR was performed using 2×Taq PCR Mix (TIANGEN Biotech) and separated by agarose gel electrophoresis.
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bioRxiv - Molecular Biology 2020Quote: ... and dH2O in a 25 μl volume using a QuantiTect SYBR Green PCR kit(TIANGEN BioTech Co.LTD, Beijing, China).Transcripts were quantified with β-actin as an internal standard ...
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bioRxiv - Biochemistry 2024Quote: ... PCR was then performed using 2×Taq PCR MasterMix II (TIANGEN Biotech) and primers flanking m5C target sites ...
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bioRxiv - Genetics 2022Quote: ... PCR reactions were performed by using 2×Taq PCR Master Mix (TIANGEN, China). KASP marker analysis was performed on the StepOnePlus Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... A 412 bp TYLCV fragment was PCR amplified using Taq PCR MasterMix (Tiangen, KT201) and primers V61 and C473 as previously described (Atzmon et al. ...
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bioRxiv - Plant Biology 2022Quote: ... Reverse transcription and quantitative real-time PCR assay were performed in a Bio-Rad iQ5 real-time system using a Quant One Step qRT-PCR (SYBR Green I) Kit (Tiangen Biotech). Transcript expression levels of FmASP were normalized to that of the reference gene ACTIN and calculated using the 2-ΔΔCt method (Livak and Schmittgen ...
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bioRxiv - Microbiology 2023Quote: ... The cDNA was synthesized for PCR reaction using a PCR master mix (Tiangen, Beijing, China). The specific amplicons were cloned into pMD18T vectors (TaKaRa ...
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bioRxiv - Cancer Biology 2024Quote: Quantitative real-time PCR (qRT-PCR) was performed using SuperReal PreMix Plus (SYBR Green) (TIANGEN) according to the manufacturer’s protocol on a Mastercycler EP gradient S RealPlex2 (Eppendorf) ...
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bioRxiv - Zoology 2024Quote: ... The RT-qPCR reactions were carried out on the PikoReal 96 Real-Time PCR System (Thermo) using the SuperReal PreMix Color (SYBR Green) kit (Tiangen, Beijing, China). Three technical replicates were applied for each biological replicate ...
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bioRxiv - Evolutionary Biology 2024Quote: ... directly pipetted into a 25-μl PCR reaction containing a 2x concentration of PCR MasterMix (Tiangen) and 10 pmol of each PCR primer ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative real-time PCR (qRT-PCR) was performed in a 20-μL reaction containing SYBR Green (Tiangen, China) on an MXP3000 cycler (Stratagene ...
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bioRxiv - Molecular Biology 2020Quote: ... quantitative real-time PCR (qRT-PCR) was performed with SYBR green as fluorescence dye (Cat# FP205-02, Tiangen Biotech, China) in a total reaction volume of 20 μL according to the standard manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 5 ul 2×Taq PCR mix (TIANGEN, China), 0.5 ul each primer (trnL and trnF (Taberlet et al. ...
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bioRxiv - Bioengineering 2020Quote: ... 10* PCR reaction buffer 5µl (Tiangen Biotech, Beijing), dTNPs 4µl ...
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bioRxiv - Microbiology 2021Quote: ... 2×Taq PCR Mix was purchased from TIANGEN Biotech (Beijing ...
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bioRxiv - Genetics 2020Quote: ... and 10 μL real-time PCR SYBR Green (TIANGEN, Beijing, China). Amplification conditions were ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative PCR was performed using SYBR Green Pre-Mix (TIANGEN, FP205) on the Roche LightCycler480 instrument ...
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bioRxiv - Cell Biology 2021Quote: ... Both digested products were then cleaned with a PCR purification column (TIANGEN), and ligation was then performed using the T4 DNA ligase (NEB) ...
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bioRxiv - Cancer Biology 2022Quote: ... qRT-PCR was performed in triplicate with FastFire qPCR PreMix (SYBR Green) (Tiangen) on ViPlex Fluor Real-time PCR system (Vivantis ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative RT-PCR was performed using the SuperReal PreMix Color (Tiangen, Beijing, China) in the CFX96 real-time system (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... Quantitative RT-PCR was performed using SYBR Green Master Mix (Tiangen, Beijing, China). The RNA expression level of target genes was evaluated by 2−ΔΔCt.
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bioRxiv - Molecular Biology 2024Quote: ... UGI fragments and overlapping-PCR mutated Cas9 variants through Gibson Assembly (TIANGEN, 4992813). The following BE plasmids were constructed ...
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bioRxiv - Microbiology 2020Quote: ... were detected by qRT-PCR using SuperReal PreMix Plus (SYBR Green) (Tiangen Biotech, China). As an endogenous control ...
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bioRxiv - Molecular Biology 2021Quote: ... The quantitative PCR was performed using Quantagene q225 (KUBOTECHNOLOGY) with SuperReal PreMix Plus (TIANGEN) according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2024Quote: ... Then Quantitative RT-PCR was carried out with SuperReal PreMix Plus SYBR Green (Tiangen) on a Roche Real-Time System (Roche 480) ...