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Citations for TianGen Biotech :
1 - 50 of 103 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: The DNA and RNA of virus were extracted by using TIANamp Virus DNA / RNA Kit (TIANGEN) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription with ZIKA virus specific primer (Rev-AAGTGATCCATGTGATCAGTTGATCC) was performed using FastQuant RT Kit (Tiangen). Real-time PCR was done on 7900HT (ABI ...
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bioRxiv - Molecular Biology 2021Quote: ... And the PRV genome within 200 μL suspension was extracted by TIANamp Virus DNA/RNA Kit (TIANGEN). All cDNAs and viral genome were -20°C.
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bioRxiv - Immunology 2024Quote: RNA from both host and virus was extracted by using the RNAsimple Total RNA Kit (DP419, TIANGEN) and Virus DNA/RNA Extraction Kit (RM401-04 ...
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bioRxiv - Physiology 2022Quote: ... Protein concentration was determined using BCA Protein Assay Kit (TIANGEN) and diluted in loading dye (β-mercaptoethanol 5% ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were quantified using a BCA Protein Assay Kit (TIANGEN, China). Western blotting analysis was performed according to previous studies [28 ...
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bioRxiv - Biochemistry 2020Quote: ... Protein concentration was measured using Bradford protein assay kit (Tiangen Biotech, Beijing Co., LTD, CN). The purity of the PM was determined by standard marker assays[32] ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... a BCA protein assay kit (TIANGEN, Beijing, China) was used to measure the membrane protein concentration ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were detected using an ECL fluorescence colorimetric kit (Tiangen) and visualized using a Bio-Rad Gel Doc XR ...
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bioRxiv - Neuroscience 2023Quote: ... Total protein concentration was measured using a BCA assay kit (TIANGEN). Then 10 mg of protein per sample was separated on 12% SDS-PAGE gels (Sangon Biotech ...
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bioRxiv - Cell Biology 2023Quote: ... the chromatin sample concentration was assessed by the BCA Protein Assay Kit (Tiangen, cat no. PA115-02). 1 μg H3K27ac antibody (Abcam ab4729 ...
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bioRxiv - Neuroscience 2021Quote: RNA was extracted from mouse brain lysates (mixture of 3∼4 mouse brains for each sample) using RNAprep pure Tissue Kit (Tiangen, DP431). cDNA was synthesized using HiScript II 1st Strand cDNA Synthesis Kit (Vazyme ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from less than 100 mg of 10-d-old seedlings that were grown vertically on half-strength MS plates or treated with 50 μM AA or an equal volume of DMSO for 4 h in liquid MS medium using RNAprep Pure Plant Plus Kit (TIANGEN, DP441). First-strand cDNA was synthesized using a HiScript® II cDNA Synthesis Kit (Vazyme R212-02 ...
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bioRxiv - Microbiology 2019Quote: ... coli DH5α competent cells (Tiangen). Four clones from each VP1 amplicon were picked for Sanger sequencing ...
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bioRxiv - Bioengineering 2023Quote: ... coli DH5α competent cells (Tiangen). Recombinant plasmids were prepared by using the QIAprep Spin Miniprep Kit (QIAGEN ...
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bioRxiv - Microbiology 2021Quote: ... of strain TC967 was extracted from 4-day-old mycelia following the protocol described in the handbook of the Plant Genomic DNA Kit (Tiangen, Beijing, China). The gDNA was sequenced by Illumina HisSeq 2500 sequencing (Illumina ...
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bioRxiv - Plant Biology 2022Quote: ... total RNAs isolated from the leaves of SC8 and mutant lines (#1, #2, #3, #4) using RNAplant Plus reagent (TianGen, Beijing, China) following the manufacturer’s instructions were reversed by using the reverse transcriptase kit (TaKaRa ...
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bioRxiv - Genomics 2023Quote: Total RNA was extracted from the developing leaves of NTS57 under normal conditions (CK) or 12 h after -4℃ treatment using the TRIzol method (Tiangen, Beijing, China) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The total protein in the supernatant was quantified using the bicinchoninic acid assay (BCA) kit (Beijing TianGen Biotechnology Co., Ltd.). For each sample ...
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bioRxiv - Molecular Biology 2020Quote: ... Isolates were cultured on Middlebrook medium for 4-6 weeks at 37°C and DNA was extracted using magnetic beads (Tiangen Biotech Co., Ltd.). Rifampicin and isoniazid drug-susceptibility testing was performed using the proportion method in Löwenstein-Jensen medium (Aziz et al ...
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bioRxiv - Cancer Biology 2024Quote: Whole cell genomic DNA was extracted from LOVO cells using a genomic DNA kit (Tiangen, DP304) and sequences from 2 kb upstream to 100 bp downstream of the gene start codon were amplified using a DNA polymerase kit ...
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bioRxiv - Bioengineering 2023Quote: ... coli BL21(DE3) competent cell (Tiangen) for protein expression.
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bioRxiv - Molecular Biology 2020Quote: Total RNA was isolated from single third-instar larva of three strains (total 30 larvae of each strain) using the DNA/RNA/Protein Isolation Kit (TianGen, Beijing, China). The first-strand cDNA was synthesized with 1 μg of total RNA using a PrimeScript™ 1st Strand cDNA Synthesis Kit with gDNA Eraser (TaKaRa Biotechnology ...
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bioRxiv - Immunology 2022Quote: ... cell pellets were re-suspended in 5 mL of red blood cell lysis buffer (RT122-02, TIANGEN, China) for 5 min at RT for remove the red blood cell ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cells were collected and lysed by TRIZOL(Tiangen) to extract RNA ...
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bioRxiv - Synthetic Biology 2020Quote: ... mRNA was extracted from the harvested cells according to the manufacturer’s instructions of a RNAprep Pure Cell/Bacteria Kit (Tiangen, Beijing, China). PrimeScript RT reagent Kit with gDNA Eraser (TaKaRa ...
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated from 1×106 THP1 cells after various treatments (see above) by using an RNAprep pure cell/bacteria kit (Tiangen Biotech 4992235) according to the manufacturer’s instructions and was quantified using NanoDrop OneC Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was isolated from cells with Trizol reagent (Tiangen); 1 μg of each RNA sample was used for first-strand cDNA synthesis with oligo (dT)18 and M-MLV reverse transcriptase (Vazyme ...
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bioRxiv - Genomics 2019Quote: ... cells were collected and subjected to RNA isolation (TIANGEN, DP430). Then ...
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bioRxiv - Genetics 2022Quote: Cells were directly lysed into RLT plus buffer (TIANGEN, DP430) for total RNA extraction according to the manufacturer’s instructions provided in the RNeasy Plus Mini Kit ...
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bioRxiv - Immunology 2019Quote: ... The ligation product was transformed into DH5α competent cell (TIANGEN, # CB101), plated on LB solid plates containing kanamycin ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from cells using TRNzol reagent (TIANGEN, China) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... The constructed plasmid was transformed into DH5α cells (CB101, Tiangen, China). Positive clones were selected and sequenced using universal primers (T7 promoter and SP6 terminator primer) ...
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bioRxiv - Microbiology 2022Quote: ... an RNA prep pure cell/bacterial kit (Tiangen Biotech, Beijing, China) was used to prepare total RNA according to the instructions ...
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bioRxiv - Neuroscience 2022Quote: ... The constructed plasmid was transformed into DH5α cells (CB101, Tiangen, China). The positive clones were selected and sequenced using universal primers (T7 promoter and SP6 terminator primer) ...
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bioRxiv - Developmental Biology 2023Quote: Tissue and cells were homogenized with 1 mL TRIZOL (TIANGEN, DP424), after which 200 μL of chloroform was added to the lysates for phase separation ...
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bioRxiv - Bioengineering 2024Quote: ... Total RNA was extracted using RNAprep Pure Cell/Bacteria Kit (TIANGEN) and reverse-transcribed into cDNA using PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time ...
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bioRxiv - Biochemistry 2020Quote: ... Total RNA was extracted using the RNAprep pure Cell/Bacteria Kit (Tiangen) following lysozyme treatment ...
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bioRxiv - Microbiology 2019Quote: ... RNA extraction was performed by RNAprep pure Cell / Bacteria Kit (Tiangen, DP430). The cDNA synthesis was performed by FastKing RT Kit (Tiangen ...
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bioRxiv - Genomics 2019Quote: ... Erythrocytes were cleared by resuspending the cells in ACK lysis buffer (Tiangen) per manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: The total RNA was extracted by the RNAprep pure Cell Kit (TIANGEN), and 1 μg RNA was reversely transcribed into cDNA by using ReverTra Ace® qPCR RT Master Mix with gDNA Remover (TOYOBO) ...
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bioRxiv - Biophysics 2021Quote: ... and HepG2-RC cells were performed by a SuperReal Kit (Tiangen, Beijing, China). The primer sequences are listed in Table S1 ...
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bioRxiv - Biochemistry 2020Quote: Total RNA was extracted from HQ461-treated A549 cells using TRNzol (Tiangen, DP405). Reverse transcription of mRNA into cDNA was performed using the 5X All-In-One RT mastermix kit (Abm,G488 ...
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bioRxiv - Genetics 2021Quote: DNA was extracted from cells using the QIAamp DNA Mini Kit (Tiangen, DP304) and quantified using NanoDrop ...
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bioRxiv - Neuroscience 2022Quote: ... the constructs were transformed into E.coli BL21 (DE3) pLysS competent cells (Tiangen, China). After growth to OD600 = 1 in a SuperBroth medium supplemented with ampicillin ...
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bioRxiv - Cell Biology 2019Quote: ... Red blood cells were lysed using RBC lysis buffer (catalogue NO. RT122-02, TIANGEN). After centrifugation (3,000 rpm ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA from cells were isolated using the RNA simple Total RNA kit (TIANGEN). 1ug RNA was reverse transcribed using a FastKing RT Kit (TIANGEN) ...
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bioRxiv - Physiology 2020Quote: ... RNA was extracted immediately using the RNAprep Pure Cell/Bacteria Kit (Tiangen Biotech (Beijing) CO. ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was extracted using an RNAprep Pure Cell/Bacteria Kit (TIANGEN, Beijing, China; DP430). Two micrograms of RNA were reverse transcribed into cDNA using a PrimeScript™ First-Strand cDNA Synthesis Kit (TaKaRa ...
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bioRxiv - Bioengineering 2022Quote: ... And then cell genomic DNA was isolated using the TIANamp Genomic Kit (Tiangen Biotech) according to the manufacturer’s instructions ...