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Citations for TianGen Biotech :
151 - 200 of 208 citations for DNA Polymerase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... The complementary DNA (cDNA) was generated using the FastKing RT kit (TIANGEN) according to the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2020Quote: ... 30 μg plasmid DNA (prepared by TIANGEN EndoFree Maxi Plasmid Kit, DP117) was used and mixed with 200 μL protoplast suspension ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the ligated cross-linked DNA was reversed using proteinase K (TIANGEN, #RT403). DNA purification was performed by removing biotin from unligated ends using T4 DNA polymerase (Enzymatics ...
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Neutralizing gut-derived lipopolysaccharide as a novel therapeutic strategy for severe leptospirosisbioRxiv - Microbiology 2024Quote: ... The pellets were extracted using the TIANamp Bacteria DNA kit (Tiangen, China) according to the manufacturer’s instructions [26] ...
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bioRxiv - Immunology 2020Quote: ... PCR products underwent agarose electrophoresis and gel-purification (Universal DNA purification kit, Tiangen). The amount of PCR products was determined by OD 260 absorption value ...
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bioRxiv - Evolutionary Biology 2023Quote: ... chamaejasme populations and three outgroups using a Plant Genomic DNA Kit (Tiangen, China). The extracted DNA was used to construct RAD-seq libraries ...
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bioRxiv - Synthetic Biology 2021Quote: ... and the DNA is extracted using the TIANgeo Midi Purification Kit (TIANGEN, DP209-02) or AxyPrep DNA gel Extraction Kit (AXYGEN ...
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bioRxiv - Genomics 2019Quote: ... QHM and BWC1 was carried out with the universal DNA purification kit from Tiangen Biotech (Beijing ...
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bioRxiv - Developmental Biology 2019Quote: ... The amplified products were purified by gel electrophoresis using Universal DNA Purification Kit (TIANGEN) and cloned into a pMD19-T vector (Takara) ...
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bioRxiv - Bioengineering 2022Quote: ... And then cell genomic DNA was isolated using the TIANamp Genomic Kit (Tiangen Biotech) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... and hydroxyurea-treated samples were extracted using a Plant Genomic DNA Kit (TIANGEN, China). Then ∼200 ng of DNA was digested with McrBC (Takara ...
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bioRxiv - Molecular Biology 2023Quote: ... and plasmid DNA was extracted with the TIANprep Mini Plasmid Kit (TIANGEN, Beijing, China) according to the instructions of the manufacturer ...
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bioRxiv - Microbiology 2024Quote: ... phototrophica AP64 was extracted using the TIANamp Genomic DNA Kit (TIANGEN Biotech, Beijing, China). For obtaining high molecular weight genomic DNA ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The DNA was extracted following the protocol of TIANquick Midi Purificatioin kit (TIANGEN Biotech, Beijing). The primers used for the DNA amplification procedure with PCR are listed in supplementary table S4 ...
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bioRxiv - Immunology 2020Quote: ... Mouse DNA was extracted from the liver using an extraction kit (DP341-01, Tiangen, China) as per the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... The marker used to indicate the fragment length is 50bp DNA Ladder (TianGen, BeiJing, China) or DL5000 (Takara ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR products underwent agarose gel electrophoresis and gel purification (Universal DNA purification kit, Tiangen, Beijing, China). For animal experiments ...
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bioRxiv - Developmental Biology 2019Quote: ... The genome extraction from mouse embryos or pups were using the TIANamp Genomic DNA Kit (TIANGEN), which used for genotyping or bisulphite sequencing.
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bioRxiv - Cell Biology 2020Quote: gDNA of LV transduced cells was extracted using the TIANamp Genomic DNA Kit (Tiangen, Beijin, China) following the manufacturer’s manual ...
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bioRxiv - Bioengineering 2023Quote: ... the PCR products were purified after agarose electrophoresis (using Universal DNA purification kit, TIANGEN, Beijing, China), and their concentrations were determined by the OD 260 value by NanoDrop (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2023Quote: ... Total genomic DNA was extracted from fresh leaves using the Hi-DNAsecure Plant Kit (TIANGEN, China). Then ...
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bioRxiv - Genetics 2019Quote: ... to extract gDNA according to the protocols described in the TIANamp blood DNA kit (Tiangen Biotech (Beijing) Co. ...
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bioRxiv - Molecular Biology 2021Quote: ... And the PRV genome within 200 μL suspension was extracted by TIANamp Virus DNA/RNA Kit (TIANGEN). All cDNAs and viral genome were -20°C.
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bioRxiv - Microbiology 2020Quote: Genomic DNA was extracted from each isolate using a gram-negative bacterial genome extraction kit (Tiangen, China). Whole-genome fragment libraries were prepared using a paired-end sample preparation kit (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... DNAs were extracted by hydroxybenzene-chloroform-isoamyl alcohol and purified by a Phase Lock Gel (Tiangen, China). For ChIP-seq ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and reverse transcribed by a quant cDNA (First strand complementary DNA) synthesis RTase Kit (Tiangen Biotech, China). Quantitative real-time polymerase chain reaction (qRT-PCR ...
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bioRxiv - Bioengineering 2021Quote: PC12 cell or rat liver gDNAs was isolated using the TIANamp blood DNA kit (TIANGEN Biotech, DP304-02) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... PCR products from each sample were separately pooled and purified with a Universal DNA Purification Kit (Tiangen, China). In the second-round PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... Nucleic acid extraction from animal tissue grinding fluid (200μL) was performed using a DNA extraction kit (TIANGEN, China) according to the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2020Quote: We extracted genomic DNA from fresh or silica gel-desiccated leaves with a DNAsecure Plant Kit (TIANGEN, Beijing, China). The primers and thermal cycling procedure were as described by Craig et al (2014) ...
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bioRxiv - Genomics 2023Quote: ... the genomic DNA was extracted from young leaves using the Hi-DNAsecure Plant Kit (cat. no. DP350; TIANGEN, China). Then ...
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bioRxiv - Genetics 2021Quote: Genomic DNA was extracted from the wings of Bmmp knockout silkworms at the adult stage using the TIANamp Genomic DNA Kit (TIANGEN). The DNA was used as a PCR template to amplify regions spanning the genomic targets ...
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bioRxiv - Evolutionary Biology 2019Quote: Total RNA was extracted using the RNAprep Pure Plant Kit and genomic DNA contamination was removed using RNase-Free DNase I (both from Tiangen). The integrity of RNA was evaluated on a 1.0% agarose gel stained with ethidium bromide (EB) ...
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bioRxiv - Genetics 2019Quote: The single-stranded viral DNA was isolated from M13mp18 and its 3kb linear dsDNA was amplified by PCR and purified by DNA purification kit (Tiangen). A 60-nt oligomer from M13 genome ...
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bioRxiv - Cancer Biology 2019Quote: ... and HL7702 cell lines after transfection with pTsgRNA-DMP-Cas9-EGFP 24 h using the TIANamp Genomic DNA Kit (Tiangen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... All DNA products were separated in agarose gel to confirm sizes and following purified using TIANgel Midi Purification Kit (TIANGEN). The mixture of 0.01 pM of backbone and 0.03 pM of each insert fragments were delivered for transformation in Stable3 competent bacteria after 30 minutes incubation in 50 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... The DNA fragments were separated by agarose gel electrophoresis and purified using a TIANgeo Midi Purification Kit (TIANGEN, DP209-02) or AxyPrep DNA gel Extraction Kit (AXYGEN ...
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bioRxiv - Microbiology 2020Quote: The genome DNA of infected soybean hairy-roots was extracted using New Plant Genome Extraction Kit (Cat: DP305, Tiangen biotech) and used for qPCR with ChamQ SYBR qPCR Master Mix (Cat ...
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bioRxiv - Genetics 2023Quote: ... and plasmids were assembled by using HiFi DNA Assembly Cloning Kit and subsequently transformed into DH5α competent cells from TIANGEN. ZymoPure Midiprep Kit (Zymo Research ...
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bioRxiv - Plant Biology 2024Quote: ... T1 or higher generation embryos and regenerated plantlets respectively using the Plant Genomic DNA isolation Kit (TianGen Biotech., Beijing, China). For T0 and T1 resistant embryos ...
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bioRxiv - Plant Biology 2019Quote: Total genomic DNA from each accession was extracted from the leaves using a Plant DNeasy Mini kit (Tiangen Biotech, Beijing, China) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Genome DNA from the collected cells of P2 was extracted under the instructions of the user’s manual for TIANamp Genomic DNA Kit (TIANGEN, DP304-02). PCR was later conducted to amplify mitochondrial COI gene fragment using the primer pair as follows ...
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bioRxiv - Plant Biology 2020Quote: ... The pCSGAPO1 vector (Cong et al., 2013) was digested with Bsa I and purified using a DNA purification kit (Tiangen, China). A ligation reaction (10 μl ...
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bioRxiv - Cell Biology 2021Quote: ... the first batch of cells (5×106) were collected 2 days after infection using the TIANamp genomic DNA kit (Tiangen, DP304), and the second batch of cells treated with puromycin were collected at 7d ...
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bioRxiv - Genomics 2022Quote: ... on an agarose gel and were subsequently extracted using a Universal DNA purification Kit (Catalog No. DP214-02, TIANGEN, Beijing, China) and then used as a template to synthesize dsRNA with a MEGAscript RNAi Kit (Catalog No ...
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bioRxiv - Bioengineering 2023Quote: Approximately 0.5 g of fresh leaf tissue from the transformed plants was subjected to total DNA extraction using the DNAquick Plant System (Tiangen Biotech), according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... A paired-end library with an insert size of 350 kb was constructed using the TIANSeq Fast DNA Library Kit (Cat. no: 4992261; Tiangen, China) and then sequenced using an Illumina NovaSeq6000 sequenator (Illumina ...
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bioRxiv - Immunology 2024Quote: ... The correct construction of the recombinant plasmids was verified through Sanger sequencing and extracted using an Endotoxin-Free Plasmid DNA Miniprep Kit (Tiangen, China) before using plasmids ...
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bioRxiv - Microbiology 2021Quote: ... of strain TC967 was extracted from 4-day-old mycelia following the protocol described in the handbook of the Plant Genomic DNA Kit (Tiangen, Beijing, China). The gDNA was sequenced by Illumina HisSeq 2500 sequencing (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was isolated from single third-instar larva of three strains (total 30 larvae of each strain) using the DNA/RNA/Protein Isolation Kit (TianGen, Beijing, China). The first-strand cDNA was synthesized with 1 μg of total RNA using a PrimeScript™ 1st Strand cDNA Synthesis Kit with gDNA Eraser (TaKaRa Biotechnology ...