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Citations for TianGen Biotech :
51 - 100 of 117 citations for hsa mir 635 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... PCR mixture contained 12 μL of 2× Taq PCR Mix (TIANGEN Co. Ltd., Beijing, PR China), 1 μL DNA template ...
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bioRxiv - Evolutionary Biology 2023Quote: ... directly pipetted into a 25-μl PCR reaction containing a 2x concentration of PCR MasterMix (Tiangen) and 10 pmol of each PCR primer ...
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bioRxiv - Microbiology 2024Quote: ... The real-time quantitative PCR (q-PCR) (ABI7500, USA) using SYBR 2×RealUniversal PreMix (Tiangen Biotech). qPCR was performed to assess the abundance of the macrolide ...
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bioRxiv - Microbiology 2021Quote: Samples of RNA subjected to RNA-seq analysis used the FastQuant RT Kit with gDNase (TIANGEN) for first-strand cDNA synthesis ...
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bioRxiv - Pathology 2020Quote: ... according to the manufacturer’s instructions and cDNAs were reverse-transcribed using the FastKing RT Kit (TIANGEN). The cDNA samples were amplified by Real-time quantitative PCR (RT-qPCR ...
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bioRxiv - Developmental Biology 2021Quote: ... it was performed in accordance with the manufacturer’s instructions using FastQuant RT Kit (With gDNase) (Tiangen). Relative mRNA levels of each gene were analyzed according to the comparative Ct method and were normalized to the expression of GAPDH ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative real-time PCR (qRT-PCR) was performed in a 20-μL reaction containing SYBR Green (Tiangen, China) on an MXP3000 cycler (Stratagene ...
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bioRxiv - Biochemistry 2021Quote: ... An aliquot of 0.5 μg RNA were reverse transcribed to cDNA by FastKing RT Kit (KR116-02, Tiangen). cDNA samples were used as templates to perform real- time quantitative PCR with the power SYBR green mix (Abm ...
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bioRxiv - Biochemistry 2019Quote: ... the first step product was converted to cDNA using FastKing RT Kit (With gDNase) (TIANGEN BIOTECH, BeiJing, China). Then ...
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bioRxiv - Molecular Biology 2020Quote: ... quantitative real-time PCR (qRT-PCR) was performed with SYBR green as fluorescence dye (Cat# FP205-02, Tiangen Biotech, China) in a total reaction volume of 20 μL according to the standard manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 5 ul 2×Taq PCR mix (TIANGEN, China), 0.5 ul each primer (trnL and trnF (Taberlet et al. ...
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bioRxiv - Bioengineering 2020Quote: ... 10* PCR reaction buffer 5µl (Tiangen Biotech, Beijing), dTNPs 4µl ...
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bioRxiv - Microbiology 2021Quote: ... 2×Taq PCR Mix was purchased from TIANGEN Biotech (Beijing ...
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bioRxiv - Cancer Biology 2022Quote: ... RT–qPCR was performed via a miRcute plus miRNA qPCR kit (SYBR Green) (Tiangen Biotech Co., Ltd., Beijing, China) on an ABI 7500 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... 2 ug RNA per sample were used to reverse transcript the cDNA by QuantScript RT Kit (TIANGEN Biotech, KR103). 2 x iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... The RNA prep pure kit and FastKing RT Kit for RNA extraction and reverse transcription were purchased from Tiangen Biothch Co. ...
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bioRxiv - Genomics 2020Quote: ... China) and first-strand cDNA was synthesized with 1µg RNA using a FastQuant RT Kit (with gDNase) (TIANGEN, Beijing, China). Gene-specific primers were designed using Primer-BLAST software (National Center for Biotechnology Information ...
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bioRxiv - Neuroscience 2020Quote: ... and then reverse transcribed using a ReverTra Ace qPCR RT Master Mix Kit (TOYOBO, Japan) and a miRcute Enhanced miRNA cDNA First-Strand Synthesis Kit (TIANGEN) according to the manufacturers’ protocols ...
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bioRxiv - Plant Biology 2022Quote: ... gDNA buffer was added into total RNA to remove DNA impurity and then the purified RNA was used as a template to synthesized cDNA using a FastKing RT Kit (TIANGEN). The RT-qPCR assays were performed on qTOWER3 Real-Time PCR thermal cyclers (Analytik Jena ...
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bioRxiv - Cancer Biology 2019Quote: ... and the Q-PCR kit was purchased from Tiangen Biotech Co. ...
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bioRxiv - Genomics 2020Quote: Synthesis of cDNA was performed with RNA products following the manufacturer’s instructions of ReverTra Ace qPCR RT Master Mix (Tiangen, Beijing, China). To validate the putative lncRNAs in V ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from cultured cells by using TRIZOL reagent and 500 ng to 2 μg RNA was reversely transcribed into first-strand cDNA by FastKing RT Kit (Tiangen, KR116). qPCR analysis was performed with Mastercycler Realplex2 (Eppendorf ...
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bioRxiv - Genetics 2024Quote: ... First-strand cDNA was synthesized from 2 μg of DNase I-treated total RNA using the FastKing RT kit (TIANGEN, KR116). qRT-PCR was performed using the ChamQ Universal SYBR qPCR Master Mix (Vazyme ...
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bioRxiv - Genetics 2019Quote: ... the PCR amplicons were purified using Gel Extraction kit (TIANGEN). A total of 200 ng of the purified PCR amplicon was denatured and slowly reannealed to facilitate heteroduplex formation ...
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bioRxiv - Genomics 2019Quote: ... The PCR products were cloned into pLB vectors (TianGen, China) and sequenced with an ABI 3730XL 96-capillary DNA analyzer (Lifetech ...
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bioRxiv - Genetics 2022Quote: ... First-strand cDNA synthesis from the total RNA was performed by using the FastKing RT kit (with gDNase) (TianGen Biotech Co, Beijing). The expression analyses were performed using the primer set (F-GGACTTCCCTTGGATCCTGC and R-ACCGACAATCATGTCCGCAT) ...
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bioRxiv - Developmental Biology 2020Quote: ... All RT-qPCR assays were performed in an ABI 7500 System with the SuperReal PreMix Plus kit (SYBR Green) (Tiangen, Beijing, China). All RT-qPCR procedures for each gene were performed in three biological replicates ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA (1 µg) was used as a template for cDNA synthesis with a QuantScript RT Kit (KR103; Tiangen Biotech, Beijing, China). To determine mRNA expression levels ...
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ARID1a protects against steatosis and insulin resistance via PPARalpha-mediated fatty acid oxidationbioRxiv - Cell Biology 2019Quote: ... DNA was purified using a PCR Purification Kit (Tiangen,Beijing,China) and subsequently analyzed by qPCR using primers located on the Acox1 and Cpt1a promoter.
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bioRxiv - Genetics 2020Quote: ... and 10 μL real-time PCR SYBR Green (TIANGEN, Beijing, China). Amplification conditions were ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative PCR was performed using SYBR Green Pre-Mix (TIANGEN, FP205) on the Roche LightCycler480 instrument ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μg of purified RNA was reverse-transcribed in a 20 µL reaction using a FastQuant RT Super Mix Kit (TIANGEN, catalog no. KR106-02). Real-Time PCR was then performed by using Maxima SYBR Green/ROX kit (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Both digested products were then cleaned with a PCR purification column (TIANGEN), and ligation was then performed using the T4 DNA ligase (NEB) ...
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bioRxiv - Cancer Biology 2022Quote: ... qRT-PCR was performed in triplicate with FastFire qPCR PreMix (SYBR Green) (Tiangen) on ViPlex Fluor Real-time PCR system (Vivantis ...
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bioRxiv - Immunology 2020Quote: ... PCR products underwent agarose electrophoresis and gel-purification (Universal DNA purification kit, Tiangen). The amount of PCR products was determined by OD 260 absorption value ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were merged and purified by using TIANquick Midi Purification Kit (TIANGEN). The NGS libraries were prepared by using VAHTS Universal DNA Library Prep Kit for Illumina (Vazyme ...
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bioRxiv - Microbiology 2020Quote: ... were detected by qRT-PCR using SuperReal PreMix Plus (SYBR Green) (Tiangen Biotech, China). As an endogenous control ...
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bioRxiv - Molecular Biology 2021Quote: ... The quantitative PCR was performed using Quantagene q225 (KUBOTECHNOLOGY) with SuperReal PreMix Plus (TIANGEN) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... The PCR products were extracted by a gel extraction kit (TIANGEN, Catalog # DP209-03). Data analysis was performed using MAGeCK.
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bioRxiv - Genomics 2019Quote: ... The PCR products were purified and cloned into the pGM-T vector (TIANGEN, Beijing, China); at least 10 positive plasmid clones were sequenced and analyzed using NCBI BLAST.
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bioRxiv - Bioengineering 2021Quote: ... The quantitative PCR was conducted using the SuperReal PreMix SYBR Green kit (Tiangen Biotech, China) and the CFX96 Real-Time system (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... Then the PCR products were purified using the Agarose Gel Extraction kit (TIANGEN, Beijing, China), and transformed into Escherichia coli DH5α ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was purified and directly recombined by the EasyGeno recombinant clone kits (Cat:VI201, Tiangen). After conventional transformation and monoclonal screening of positive bacteria ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR products underwent agarose gel electrophoresis and gel purification (Universal DNA purification kit, Tiangen, Beijing, China). For animal experiments ...
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bioRxiv - Bioengineering 2023Quote: ... the PCR products were purified after agarose electrophoresis (using Universal DNA purification kit, TIANGEN, Beijing, China), and their concentrations were determined by the OD 260 value by NanoDrop (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... The sgRNA-targeted loci of Lmna gene were amplified using Taq PCR MasterMix (KT211, Tiangen, China) and purified by TIANgel Purification Kit (DP219 ...
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bioRxiv - Molecular Biology 2023Quote: ... The sgRNA-targeted loci of Palmd gene were amplified using Taq PCR MasterMix (KT211, Tiangen, China) and purified by TIANgel Purification Kit (DP219 ...
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bioRxiv - Microbiology 2020Quote: ... One step Qrt-PCR reaction was performed using the Tiangen One-step SYBR Green kit (Tiangen, China) in the Applied Biosystems QuantStudio 6 Flex RT-PCR System.
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bioRxiv - Immunology 2022Quote: ... cDNA synthesis was executed with 1 µg of total RNA with BcaBEST RNA PCR Kit (TIANGEN, China) as the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... the products of first round extension PCR were purified by gel extraction kit (TIANGEN Biotech, Beijing, China), of which 5 μL of each sample was used as the template in the second extension PCR ...