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Citations for TianGen Biotech :
451 - 500 of 576 citations for Thyroxine ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2019Quote: Total RNA was extracted using the RNAprep Pure Plant Kit and genomic DNA contamination was removed using RNase-Free DNase I (both from Tiangen). The integrity of RNA was evaluated on a 1.0% agarose gel stained with ethidium bromide (EB) ...
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bioRxiv - Genetics 2019Quote: The single-stranded viral DNA was isolated from M13mp18 and its 3kb linear dsDNA was amplified by PCR and purified by DNA purification kit (Tiangen). A 60-nt oligomer from M13 genome ...
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bioRxiv - Genetics 2019Quote: ... genomic DNA was abstracted from 200ul blood of each participant by making use of TIANamp Blood DNA Kit (Centrifugal column, TIANGEN). Positive and negative samples were put into 384-well plates ...
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bioRxiv - Cancer Biology 2019Quote: ... and HL7702 cell lines after transfection with pTsgRNA-DMP-Cas9-EGFP 24 h using the TIANamp Genomic DNA Kit (Tiangen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... and Genomic DNA from all the fungal isolates was extracted using DNA extraction kit (TIANGEN Co. Ltd., Beijing, PR China). The internal transcribed spacer (ITS ...
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bioRxiv - Neuroscience 2021Quote: ... All DNA products were separated in agarose gel to confirm sizes and following purified using TIANgel Midi Purification Kit (TIANGEN). The mixture of 0.01 pM of backbone and 0.03 pM of each insert fragments were delivered for transformation in Stable3 competent bacteria after 30 minutes incubation in 50 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... The DNA fragments were separated by agarose gel electrophoresis and purified using a TIANgeo Midi Purification Kit (TIANGEN, DP209-02) or AxyPrep DNA gel Extraction Kit (AXYGEN ...
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bioRxiv - Microbiology 2020Quote: ... Total genomic DNA from each sample was extracted using the TIANamp Bacteria DNA isolation kit (DP302-02, Tiangen, Beijing, China). The quality of the extracted DNA was monitored on 1% agarose gels using electrophoresis and spectrophotometry (optical density at 260/280 nm ratio) ...
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bioRxiv - Microbiology 2020Quote: The genome DNA of infected soybean hairy-roots was extracted using New Plant Genome Extraction Kit (Cat: DP305, Tiangen biotech) and used for qPCR with ChamQ SYBR qPCR Master Mix (Cat ...
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bioRxiv - Molecular Biology 2022Quote: ... and the total RNA and nuclear RNA were extracted using RNAprep Pure Plant Plus Kit (Polysaccharides & Polyphenolics-rich, TIANGEN, DP441) according to the manufacturer’s instructions.
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bioRxiv - Pathology 2022Quote: ... Plant total RNA were extracted according to the instruction of the polysaccharide polyphenol plant total RNA extraction kit (Tiangen: DP441). The contaminating DNA was eliminated using RNase-free DNase and the first-strand cDNA was synthesized using Revert Aid First Strand cDNA Synthesis Kit (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... The lncRNA was reverse transcribed into cDNA using a lnRcute lncRNA First-Strand cDNA kit (4992908; Tiangen Biotech, Beijing, China) and quantified using a lnRcute lncRNA qPCR kit (4992886 ...
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bioRxiv - Plant Biology 2021Quote: ... Reverse transcription of miRNAs into cDNAs was carried out using the miRcute Plus miRNA First-Stand cDNA Synthesis Kit (Tiangen). Quantitative real-time PCR was performed using the 7500 Fast Real-Time PCR engine (Applied Biosystems ...
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bioRxiv - Plant Biology 2021Quote: ... and pUBQ10:MSD2ΔSP-mCherry. Mutagenesis of MSD2/Y68F was conducted with the Fast Site-Directed Mutagenesis Kit (cat. n. KM101) from TianGen. The constructs were introduced into plants by the floral dip method [21] ...
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bioRxiv - Plant Biology 2021Quote: The roots of 7 days old seedlings were collected to extract total RNA using RNA prep pure plant kit (TIANGEN). The first strand cDNA was synthesized from 1 μg of total RNA using the Hifair® III 1st Strand cDNA Synthesis SuperMix kit (YEASEN ...
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bioRxiv - Plant Biology 2019Quote: ... 0.1 g of powder was used for isolating the mRNA with the RNAprep pure Plant Kit (Cat#DP432, TIANGEN, Beijing), RNA was quality check the total RNA with the 2% agrose gel ...
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bioRxiv - Bioengineering 2019Quote: ... the GFP positive cells were sorted out by MoFlo XDP machine and the genomic DNA was isolated using TIANamp Genomic DNA Kit (TIANGEN) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was prepared from whole seedlings (14 DAG) using the RNA Prep Pure Plant Kit (Tiangen Biotech, Beijing China). 2 µg of RNA was used for the preparation of cDNA using PrimeScript Reagent Kit with gDNA eraser (Takara ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: PBMCs isolated from blood samples were extracted with total RNA using the Trizol method (total RNA Extraction Kit, DP431, Tiangen), and the purity NanoDrop2000 ...
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bioRxiv - Genomics 2020Quote: ... China) and first-strand cDNA was synthesized with 1µg RNA using a FastQuant RT Kit (with gDNase) (TIANGEN, Beijing, China). Gene-specific primers were designed using Primer-BLAST software (National Center for Biotechnology Information ...
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bioRxiv - Neuroscience 2020Quote: Total RNA was extracted from the rat hippocampus after various treatments using an RNA-simple Total RNA Kit (TIANGEN, China) and then reverse transcribed using a ReverTra Ace qPCR RT Master Mix Kit (TOYOBO ...
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bioRxiv - Microbiology 2021Quote: Total RNA of formalin-fixed paraffin-embedded (FFPE) explanted lungs from lung transplantation of two COVID-19 patients was extracted through RNAprep Pure FFPE Kit (TIANGEN), and used for the further verification of the two identified SARS-CoV-2-Encoded svRNAs ...
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bioRxiv - Genomics 2021Quote: ... Total DNA was acquired from 100mg fresh young leaves using a plant genomic DNA kit (Tiangen Biotech, Beijing, Co., Ltd.). Finally ...
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bioRxiv - Biochemistry 2022Quote: Total RNAs were prepared from cells using the RNA Simple Total RNA Kit (DP419, Tiangen Biotech Co., Ltd., Beijing, China), and reverse transcription was performed using the Revert Aid First Strand cDNA Synthesis Kit from Thermo ...
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bioRxiv - Genetics 2022Quote: Genomic DNAs were collected from one small piece of ear tissues of cloned newborn piglets using TIANamp Genomic DNA Kit (TIANGEN). Then the genomic DNAs were used as a PCR template and PCR products were analyzed by agarose gel electrophoresis for identification of the cloned piglet genotypes ...
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bioRxiv - Molecular Biology 2023Quote: RBPs of interest were cloned into pcDNA3-3HA-hADAR2cd-E488Q13 to build pcDNA3-3HA-RBP-hADAR2cd-E488Q using Gibson assembly method46 (EasyGeno Single Assembly Cloning kit, Tiangen). PGK1 and CYC1 were acquired from yeast gDNA ...
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bioRxiv - Plant Biology 2022Quote: ... gDNA buffer was added into total RNA to remove DNA impurity and then the purified RNA was used as a template to synthesized cDNA using a FastKing RT Kit (TIANGEN). The RT-qPCR assays were performed on qTOWER3 Real-Time PCR thermal cyclers (Analytik Jena ...
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bioRxiv - Plant Biology 2024Quote: ... T1 or higher generation embryos and regenerated plantlets respectively using the Plant Genomic DNA isolation Kit (TianGen Biotech., Beijing, China). For T0 and T1 resistant embryos ...
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bioRxiv - Plant Biology 2024Quote: The expression of DEGs identified by the transcriptomic analysis and the expression of the key genes involved in flavonoid/anthocyanin biosynthesis was validated by RT qPCR using a FastKing RT Kit from Tiangen Co ...
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bioRxiv - Zoology 2024Quote: The CRKP isolates were subjected to total genomic DNA extraction using the TIANamp Bacteria DNA Kit (Tiangen Biotech, Beijing, China) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... The total protein in the supernatant was quantified using the bicinchoninic acid assay (BCA) kit (Beijing TianGen Biotechnology Co., Ltd.). For each sample ...
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bioRxiv - Cancer Biology 2023Quote: ... total RNA from the cells plated on 96 well plates were first extracted and purified using the RNAprep Pure Micro Kit (TIANGEN). Reverse transcription was performed with HiScript III cDNA Synthesis Kit (Vazyme) ...
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bioRxiv - Biophysics 2022Quote: ... the PCR amplified PAM-containing products were purified by VAHTS DNA Clean Beads (Vazyme) and further amplified by TIANSeq Fast DNA Library Prep Kit (TIANGEN) for Illumina Novaseq PE150 sequencing ...
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bioRxiv - Genetics 2023Quote: ... and plasmids were assembled by using HiFi DNA Assembly Cloning Kit and subsequently transformed into DH5α competent cells from TIANGEN. ZymoPure Midiprep Kit (Zymo Research ...
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bioRxiv - Plant Biology 2024Quote: ... the sequence encoding full-length ICE1 was cloned into pET28a using ClonExpress II One Step Cloning Kit (Vazyme) and transformed into Escherichia coli strain BL21 (DE3) (TIANGEN). The protein was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Plant Biology 2019Quote: Total genomic DNA from each accession was extracted from the leaves using a Plant DNeasy Mini kit (Tiangen Biotech, Beijing, China) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Genome DNA from the collected cells of P2 was extracted under the instructions of the user’s manual for TIANamp Genomic DNA Kit (TIANGEN, DP304-02). PCR was later conducted to amplify mitochondrial COI gene fragment using the primer pair as follows ...
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bioRxiv - Evolutionary Biology 2022Quote: Genomic DNA (gDNA) was isolated from adductor muscle tissues in parents and offspring using the TIANamp Marine Animals DNA Kit (TIANGEN, Beijing). Library preparation and high-throughput sequencing were conducted by Novogene (Beijing ...
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bioRxiv - Genetics 2019Quote: ... the template DNA was linearized by XbaI digestion (for Cas9 and qCas9 mRNA) or NotI digestion (for EGFP and EGFP-PEST mRNA) and then purified by TIANquick Mini Purification Kit (TIANGEN Biotech). The capped Cas9/qCas9 and EGFP/EGFP-PEST mRNA were synthesized by mMESSAGE mMACHINE T7 or SP6 kit (Invitrogen) ...
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bioRxiv - Biochemistry 2019Quote: ... The extraction of total RNA from inoculated and non-inoculated control samples was carried-out with RNAprep pure Plant Kit (Tiangen, Beijing) by following processes recommended by the manufacturer ...
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bioRxiv - Synthetic Biology 2020Quote: ... mRNA was extracted from the harvested cells according to the manufacturer’s instructions of a RNAprep Pure Cell/Bacteria Kit (Tiangen, Beijing, China). PrimeScript RT reagent Kit with gDNA Eraser (TaKaRa ...
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bioRxiv - Plant Biology 2020Quote: ... The pCSGAPO1 vector (Cong et al., 2013) was digested with Bsa I and purified using a DNA purification kit (Tiangen, China). A ligation reaction (10 μl ...
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bioRxiv - Plant Biology 2021Quote: ... and the leaf samples were pooled for each group for DNA extraction using the TIANGEN DNA purification kit (TIANGEN, DP305-02). High-throughput sequencing and Bulked Sequence Analysis (BSA ...
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bioRxiv - Neuroscience 2021Quote: RNA was extracted from mouse brain lysates (mixture of 3∼4 mouse brains for each sample) using RNAprep pure Tissue Kit (Tiangen, DP431). cDNA was synthesized using HiScript II 1st Strand cDNA Synthesis Kit (Vazyme ...
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bioRxiv - Cell Biology 2021Quote: ... the first batch of cells (5×106) were collected 2 days after infection using the TIANamp genomic DNA kit (Tiangen, DP304), and the second batch of cells treated with puromycin were collected at 7d ...
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bioRxiv - Genomics 2022Quote: ... on an agarose gel and were subsequently extracted using a Universal DNA purification Kit (Catalog No. DP214-02, TIANGEN, Beijing, China) and then used as a template to synthesize dsRNA with a MEGAscript RNAi Kit (Catalog No ...
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bioRxiv - Plant Biology 2022Quote: ... Reverse transcription and quantitative real-time PCR assay were performed in a Bio-Rad iQ5 real-time system using a Quant One Step qRT-PCR (SYBR Green I) Kit (Tiangen Biotech). Transcript expression levels of FmASP were normalized to that of the reference gene ACTIN and calculated using the 2-ΔΔCt method (Livak and Schmittgen ...
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bioRxiv - Plant Biology 2022Quote: ... the young leaves of eight to ten seedlings were sampled and pooled for genomic DNA extraction using the Plant Genomic DNA Extraction Kit (DP205, TianGen, China) following the instructions provided by the supplier.
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from less than 100 mg of 10-d-old seedlings that were grown vertically on half-strength MS plates or treated with 50 μM AA or an equal volume of DMSO for 4 h in liquid MS medium using RNAprep Pure Plant Plus Kit (TIANGEN, DP441). First-strand cDNA was synthesized using a HiScript® II cDNA Synthesis Kit (Vazyme R212-02 ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from leaf and root tissues of the natural population and transgenic rapeseed using RNAsimple Kit (DP419, TIANGEN, China). The RNA was sequenced using Illumina 2500 platform (Illumina Inc ...