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Citations for TianGen Biotech :
451 - 500 of 578 citations for Onion Yellow Dwarf Virus OYDV PCR Kits since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: Total RNA from the seeds was isolated using the RNAprep Pure Plant Kit (Polysaccharides and Polyphenolics, Tiangen, Beijng, China) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... total RNAs were extracted from seedlings or different plant tissues by RNA Easy Fast Plant Tissue Kit (TIANGEN, DP452) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA of 3-week-old SsSINA1a-overexpressing Arabidopsis was extracted using an RNA Extraction Kit (DP411, Tiangen Biotech). The housekeeping genes AtACTIN was used as the internal control ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA extraction from each sample employed the RNA Easy Fast Cell Total RNA Extraction Kit (DP451, Tiangen, Beijing), following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted by using an RNA Prep Pure Plant Kit (polysaccharide and polyphenolic-rich) (TIANGEN, Beijing, China) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: Genomic DNA was isolated from the iPS cell line using the Magetic Univeral Genomic DNA kit (TIANGEN, DP705-01). Copy numbers of ampliconic genes were validated through droplet digital PCR (ddPCR) ...
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bioRxiv - Zoology 2023Quote: ... total genomic DNA was extracted from muscle tissue of pre-thoraxes using Tissue DNA kit (TIANGEN Biotech, Beijing, China) according to the manufacture’s protocol ...
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bioRxiv - Genomics 2023Quote: ... the genomic DNA was extracted from young leaves using the Hi-DNAsecure Plant Kit (cat. no. DP350; TIANGEN, China). Then ...
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bioRxiv - Cancer Biology 2024Quote: ... The K20R and K20Q mutant AKT1 plasmids were constructed using the Fast Site-Directed Mutagenesis Kit (TIANGEN, Cat# KM101). All plasmids used in this article were confirmed by Sanger sequencing.
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bioRxiv - Genetics 2024Quote: The genomic DNA from blood of each newborn piglet was extracted using TIANamp genomic DNA kit (Tiangen, Beijing, China) and PCR was carried out using 2×Ex Taq Master Mix (TaKaRa CW0718) ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was synthesized using 1 µg of total RNA by utilizing the FastQuant RT Kit (with gDNase) (TIANGEN KR106). Synthesized cDNAs were then quantified by qRT‒PCR with PerfectStart® Green qPCR SuperMix (TRAN AQ601 ...
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bioRxiv - Microbiology 2024Quote: ... The total RNA of all the samples was extracted using RNAprep Pure Cell/Bacteria Kit (Tiangen Biotech, Beijing, China) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Genomic DNA from these 50 samples was extracted from leaves using the Magnetic Plant Genomic DNA Kit (Tiangen, China). Paired-end libraries were constructed for all samples ...
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bioRxiv - Molecular Biology 2020Quote: Experimental cells were subjected to isolation of total RNAs by using the RNA simple Kit (Tiangen Biotech Co., Beijing, China). Then ...
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bioRxiv - Genetics 2021Quote: Genomic DNA was extracted from the wings of Bmmp knockout silkworms at the adult stage using the TIANamp Genomic DNA Kit (TIANGEN). The DNA was used as a PCR template to amplify regions spanning the genomic targets ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA from the different tissue and leaf axil specimens was extracted with an RNAprep Pure Plant Kit (TIANGEN, China) according to the kit protocol ...
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bioRxiv - Neuroscience 2021Quote: ... All DNA products were separated in agarose gel to confirm sizes and following purified using TIANgel Midi Purification Kit (TIANGEN). The mixture of 0.01 pM of backbone and 0.03 pM of each insert fragments were delivered for transformation in Stable3 competent bacteria after 30 minutes incubation in 50 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... The DNA fragments were separated by agarose gel electrophoresis and purified using a TIANgeo Midi Purification Kit (TIANGEN, DP209-02) or AxyPrep DNA gel Extraction Kit (AXYGEN ...
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bioRxiv - Microbiology 2020Quote: ... Total genomic DNA from each sample was extracted using the TIANamp Bacteria DNA isolation kit (DP302-02, Tiangen, Beijing, China). The quality of the extracted DNA was monitored on 1% agarose gels using electrophoresis and spectrophotometry (optical density at 260/280 nm ratio) ...
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bioRxiv - Microbiology 2020Quote: The genome DNA of infected soybean hairy-roots was extracted using New Plant Genome Extraction Kit (Cat: DP305, Tiangen biotech) and used for qPCR with ChamQ SYBR qPCR Master Mix (Cat ...
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bioRxiv - Molecular Biology 2022Quote: ... and the total RNA and nuclear RNA were extracted using RNAprep Pure Plant Plus Kit (Polysaccharides & Polyphenolics-rich, TIANGEN, DP441) according to the manufacturer’s instructions.
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bioRxiv - Pathology 2022Quote: ... Plant total RNA were extracted according to the instruction of the polysaccharide polyphenol plant total RNA extraction kit (Tiangen: DP441). The contaminating DNA was eliminated using RNase-free DNase and the first-strand cDNA was synthesized using Revert Aid First Strand cDNA Synthesis Kit (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... The lncRNA was reverse transcribed into cDNA using a lnRcute lncRNA First-Strand cDNA kit (4992908; Tiangen Biotech, Beijing, China) and quantified using a lnRcute lncRNA qPCR kit (4992886 ...
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bioRxiv - Plant Biology 2021Quote: ... Reverse transcription of miRNAs into cDNAs was carried out using the miRcute Plus miRNA First-Stand cDNA Synthesis Kit (Tiangen). Quantitative real-time PCR was performed using the 7500 Fast Real-Time PCR engine (Applied Biosystems ...
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bioRxiv - Plant Biology 2021Quote: ... and pUBQ10:MSD2ΔSP-mCherry. Mutagenesis of MSD2/Y68F was conducted with the Fast Site-Directed Mutagenesis Kit (cat. n. KM101) from TianGen. The constructs were introduced into plants by the floral dip method [21] ...
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bioRxiv - Plant Biology 2021Quote: The roots of 7 days old seedlings were collected to extract total RNA using RNA prep pure plant kit (TIANGEN). The first strand cDNA was synthesized from 1 μg of total RNA using the Hifair® III 1st Strand cDNA Synthesis SuperMix kit (YEASEN ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: PBMCs isolated from blood samples were extracted with total RNA using the Trizol method (total RNA Extraction Kit, DP431, Tiangen), and the purity NanoDrop2000 ...
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bioRxiv - Genomics 2020Quote: ... China) and first-strand cDNA was synthesized with 1µg RNA using a FastQuant RT Kit (with gDNase) (TIANGEN, Beijing, China). Gene-specific primers were designed using Primer-BLAST software (National Center for Biotechnology Information ...
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bioRxiv - Neuroscience 2020Quote: Total RNA was extracted from the rat hippocampus after various treatments using an RNA-simple Total RNA Kit (TIANGEN, China) and then reverse transcribed using a ReverTra Ace qPCR RT Master Mix Kit (TOYOBO ...
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bioRxiv - Microbiology 2021Quote: Total RNA of formalin-fixed paraffin-embedded (FFPE) explanted lungs from lung transplantation of two COVID-19 patients was extracted through RNAprep Pure FFPE Kit (TIANGEN), and used for the further verification of the two identified SARS-CoV-2-Encoded svRNAs ...
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bioRxiv - Genomics 2021Quote: ... Total DNA was acquired from 100mg fresh young leaves using a plant genomic DNA kit (Tiangen Biotech, Beijing, Co., Ltd.). Finally ...
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bioRxiv - Biochemistry 2022Quote: Total RNAs were prepared from cells using the RNA Simple Total RNA Kit (DP419, Tiangen Biotech Co., Ltd., Beijing, China), and reverse transcription was performed using the Revert Aid First Strand cDNA Synthesis Kit from Thermo ...
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bioRxiv - Genetics 2022Quote: Genomic DNAs were collected from one small piece of ear tissues of cloned newborn piglets using TIANamp Genomic DNA Kit (TIANGEN). Then the genomic DNAs were used as a PCR template and PCR products were analyzed by agarose gel electrophoresis for identification of the cloned piglet genotypes ...
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bioRxiv - Molecular Biology 2023Quote: RBPs of interest were cloned into pcDNA3-3HA-hADAR2cd-E488Q13 to build pcDNA3-3HA-RBP-hADAR2cd-E488Q using Gibson assembly method46 (EasyGeno Single Assembly Cloning kit, Tiangen). PGK1 and CYC1 were acquired from yeast gDNA ...
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bioRxiv - Plant Biology 2022Quote: ... gDNA buffer was added into total RNA to remove DNA impurity and then the purified RNA was used as a template to synthesized cDNA using a FastKing RT Kit (TIANGEN). The RT-qPCR assays were performed on qTOWER3 Real-Time PCR thermal cyclers (Analytik Jena ...
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bioRxiv - Plant Biology 2024Quote: ... T1 or higher generation embryos and regenerated plantlets respectively using the Plant Genomic DNA isolation Kit (TianGen Biotech., Beijing, China). For T0 and T1 resistant embryos ...
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bioRxiv - Plant Biology 2024Quote: The expression of DEGs identified by the transcriptomic analysis and the expression of the key genes involved in flavonoid/anthocyanin biosynthesis was validated by RT qPCR using a FastKing RT Kit from Tiangen Co ...
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bioRxiv - Zoology 2024Quote: The CRKP isolates were subjected to total genomic DNA extraction using the TIANamp Bacteria DNA Kit (Tiangen Biotech, Beijing, China) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... The total protein in the supernatant was quantified using the bicinchoninic acid assay (BCA) kit (Beijing TianGen Biotechnology Co., Ltd.). For each sample ...
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bioRxiv - Cancer Biology 2023Quote: ... total RNA from the cells plated on 96 well plates were first extracted and purified using the RNAprep Pure Micro Kit (TIANGEN). Reverse transcription was performed with HiScript III cDNA Synthesis Kit (Vazyme) ...
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bioRxiv - Biophysics 2022Quote: ... the PCR amplified PAM-containing products were purified by VAHTS DNA Clean Beads (Vazyme) and further amplified by TIANSeq Fast DNA Library Prep Kit (TIANGEN) for Illumina Novaseq PE150 sequencing ...
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bioRxiv - Genetics 2023Quote: ... and plasmids were assembled by using HiFi DNA Assembly Cloning Kit and subsequently transformed into DH5α competent cells from TIANGEN. ZymoPure Midiprep Kit (Zymo Research ...
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bioRxiv - Plant Biology 2024Quote: ... the sequence encoding full-length ICE1 was cloned into pET28a using ClonExpress II One Step Cloning Kit (Vazyme) and transformed into Escherichia coli strain BL21 (DE3) (TIANGEN). The protein was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Microbiology 2024Quote: ... Protein concentrations were determined using the BCA Protein Assay Kit according to the manufacturer’s instructions (TIANGEN, catalog number: PA115-02).
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bioRxiv - Bioengineering 2024Quote: ... NECC organoids treated with 0.1 μM Quisinostat 2HCl or PBS for 48 h were isolated using the RNAprep Pure Micro Kit (Tiangen Biotech) and then sent for sequencing (Novogene) ...
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bioRxiv - Genetics 2024Quote: ... the leaves of L20191212 and 4100 were collected and total RNA was extracted as described in the miRNeasy Mini Kit (Cat: 217004; TIANGEN). Consequently ...
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bioRxiv - Genomics 2024Quote: ... Leaves of plants grown at the E3 stage40 were collected and pooled for DNA extraction using the DNAsecure Plant Kit (TIANGEN). For Illumina short-reads sequencing ...
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bioRxiv - Evolutionary Biology 2024Quote: ... was extracted from transfected HEK-293T from each subline (1×105) with the TIANamp genomic DNA Kit (DP304-02; Tiangen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Genome DNA from the collected cells of P2 was extracted under the instructions of the user’s manual for TIANamp Genomic DNA Kit (TIANGEN, DP304-02). PCR was later conducted to amplify mitochondrial COI gene fragment using the primer pair as follows ...
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bioRxiv - Evolutionary Biology 2022Quote: Genomic DNA (gDNA) was isolated from adductor muscle tissues in parents and offspring using the TIANamp Marine Animals DNA Kit (TIANGEN, Beijing). Library preparation and high-throughput sequencing were conducted by Novogene (Beijing ...