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Citations for System Biosciences :
1 - 50 of 73 citations for Sheep Anti CMV Pentamer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... into XPack CMV constructs (System Biosciences). The cyclin D1-GFP plasmids were constructed by combining the PCR fragment of cyclin D1 from the cyclin D1-Flag plasmid and GFP from pEGFP-N1 (BD Biosciences Clontech ...
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bioRxiv - Cell Biology 2021Quote: ... into XPack CMV constructs (System Biosciences). For cyclin D1 overexpression ...
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bioRxiv - Biochemistry 2021Quote: ... pCDH-CMV-MCS-EF1-Puro (System Biosciences) for the viral infection and expression of N-terminal FLAG-tagged USP8 ...
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bioRxiv - Immunology 2021Quote: ... pCDH-CMV-MCS-EF1α-RFP (System Biosciences). To facilitate the secretion and purification of the protein ...
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bioRxiv - Cell Biology 2022Quote: ... The pCDH-CMV-MCS-EF1-Blast was generated from pCDH-CMV-MCS-EF1-Hygro (plasmid #CD515B-1; System Biosciences). The annealed synthetic oligonucleotides (Table 2 ...
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bioRxiv - Biophysics 2022Quote: ... PB-CMV-MCS-EF1α-Puro (System Bioscience #PB510B-1) was linearized using EcoRI and BamHI ...
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bioRxiv - Cell Biology 2019Quote: ... PiggyBac vector PB-CMV-MCS-EF1a-Puro (System Biosciences) was first modified to include blasticidin resistance gene instead of the puromycin one and linearized using SfiI and XbaI ...
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bioRxiv - Neuroscience 2022Quote: ... digested pCDH-CMV-MSC-T2A-EGFP vector (System Biosciences). mAKT1 (Merck ...
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bioRxiv - Neuroscience 2023Quote: ... into PB-CMV-MCS-EF1-Puro (System Biosciences #PB510B-1).
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bioRxiv - Neuroscience 2023Quote: ... into PB-CMV-MCS-EF1-Puro (System Biosciences #PB510B-1).
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bioRxiv - Neuroscience 2023Quote: ... into PB-CMV-MCS-EF1-Puro (System Biosciences #PB510B-1).
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bioRxiv - Cell Biology 2019Quote: ... was transferred into pCDH-CMV-MCS-EF1-Puro (System Biosciences, cat #CD510B-1) using NheI/NotI sites for virus production.
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bioRxiv - Cancer Biology 2022Quote: We obtained pCDH-CMV-MCS-EF1α-Puro lentivector from System Biosciences (#CD510B-1). To generate the pCDH-NT5C2 expression construct ...
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bioRxiv - Cell Biology 2020Quote: ... was transferred into pCDH-CMV-MCS-EF1-Puro (System Biosciences, cat #CD510B-1) using NheI/NotI sites for virus production.
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bioRxiv - Cancer Biology 2023Quote: ... plasmid pCDH-CMV-MCS2-EF1-Hygro (System Biosciences Inc., Palo Alto, CA, USA) cloned with Luc2 open reading frame (ORF ...
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bioRxiv - Microbiology 2021Quote: ... Ceg3 was inserted into pCDH-CMV-MCS-EF1a-RFP (System Biosciences cat# CD512B-1), which was transfected together with pMD2.G (Addgene plasmid #12259 ...
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bioRxiv - Cell Biology 2021Quote: ... into the XPack CMV-XP-MCS-EF1α-Puro Cloning Lentivector (System Biosciences, Palo Alto, CA). The cyclin D1-APEX plasmids were constructed by combining the PCR fragment of cyclin D1 from the cyclin D1-Flag plasmid and APEX from pcDNA3 APEX-nes (Addgene ...
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bioRxiv - Cell Biology 2021Quote: ... the cyclin D1 was generated by PCR insertion into the XPack CMV constructs (System Biosciences). Hsc70D10N was generated by PCR-based site-directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) ...
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bioRxiv - Systems Biology 2020Quote: ... GFP gene was amplified from pGreenPuro shRNA Cloning and Expression Lentivector (CMV; System biosciences, SI505A-1) and sub-cloned into ITR-CAG-DEST-IRES-Neomycin-ITR plasmid (generous gift from Marc Supprian Schmidt) ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: ... It was thereafter inserted into pCDH-CMV-MCS-EF1-puro vector (System Biosciences, Palo Alto, CA), resulting in vector mNeonGreen-puro ...
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bioRxiv - Cancer Biology 2023Quote: Mouse SIX4 cDNA (Genomics-online) was cloned into pCDH-CMV-MCS-EF1α-Puro Lentivector (System Biosciences). Human SIX4 cDNA was amplified from genomic DNA by PCR and cloned into pCDH-CMV-MCS-EF1α-Puro vector.
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bioRxiv - Cancer Biology 2023Quote: ... A second ESR1-DAB2 plasmid in a pCDH-CMV-MCS-ER1α-Puro (System Biosciences #CD510B-1) was received as a kind gift from Dr ...
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bioRxiv - Microbiology 2020Quote: ... cDNA sequences were cloned into the PB-CMV-MCS-EF1α-Puro vector (PB510B-1, SBI System Biosciences) where the CMV promoter was replaced by a PGK promoter ...
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bioRxiv - Microbiology 2021Quote: ... the gfp-lem8 fusion was inserted into pCDH-CMV-MCS-EF1a-Puro (System Biosciences, cat# CD510B-1). The plasmids were co-transfected with pMD2.G (gift from Dr ...
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bioRxiv - Cancer Biology 2022Quote: ... 34kDa isoform of PIM1 in a pCDH-CMV-MCS-EF1α-GreenPuro Cloning and Expression Lentivector(System Biosciences) via the Spinfection method ...
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bioRxiv - Cancer Biology 2019Quote: ... Lentiviral vectors for GFP-FAK wildtype and FAK kinase-dead (R454) in pCDH-CMV-MCS-Puro (System Biosciences) were used as described (Chen et al. ...
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bioRxiv - Cancer Biology 2019Quote: ... RNS85 and RNS87 were stably transfected at MOI = 0.3 with pCDH-CMV-MCS-EF1-Puro-copGFP (System biosciences) empty vector (EV) ...
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bioRxiv - Cell Biology 2021Quote: The backbone of the original plasmid pCDH-CMV-MCS-EF1-copGFP-T2A-Puro (System Biosciences, Palo Alto, USA) was amplified and linearized via PCR using the high-fidelity PrimeSTAR GXL DNA Polymerase (Takara Bio ...
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bioRxiv - Cancer Biology 2019Quote: Overexpression vectors were constructed using pCDH-CMV-MCS-EF1-GFP cloning/ expression vector (System Biosciences, Palo Alto, CA,USA). C/EBPδ insert was cut out of a PCR4 TOPO cloning vector (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... Both human and mouse BGN were subcloned into the PB-CMV-MCS-EF1α-Puro piggybac expression vector (System Biosciences) using the 5’ EcoRI and 3’ BamHI restriction sites ...
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bioRxiv - Systems Biology 2023Quote: ... All of the Notch receptor piggyBac constructs were derived from the vector PB-CMV-MCS-EF1-Puro (System Biosciences), with changes made to the promoter (CMV changed to PGK or CAG ...
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bioRxiv - Genetics 2023Quote: The PB EGFP donor vector (PB-CMV-MCS-EF1ɑ-GFP, #PB511B-1) was purchased from System Biosciences (Palo Alto, CA). The PiggyBac transposase (PBase ...
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bioRxiv - Cell Biology 2023Quote: The CRISPR guide RNAs were cloned into the CMV-hspCas9-H1-gRNA SmartNuclease vector (System Biosciences, Palo Alto, CA, USA). The Cas9-target sequences for PPARγ-binding sites are as follows ...
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bioRxiv - Microbiology 2020Quote: ... two modified PiggyBac (PB) transposon vectors (MIHIPsMie and EIRBsMie) were constructed based on PB-CMV-MCS-EF1α-RedPuro (System Biosciences, PB514B2). The fragment of CMV-MCS-EF1α-RedPuro on this vector was removed by SfiI/ApaI digestion ...
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bioRxiv - Cancer Biology 2020Quote: The piggyBac transposon vector was constructed by inserting a random 10-bp DNA barcode sequence between the CMV promoter and the multiple cloning site of the piggyBac dual promoter vector (PB513B-1; System Biosciences). The GFP sequence in the piggyBac vector was deleted by a site-directed mutagenesis technique for the direct repeat–green fluorescent protein (DR-GFP ...
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bioRxiv - Microbiology 2021Quote: The gene for human HBEGF was synthesized and cloned into the MCS of PiggyBac vector PB-CMV-MCS-EF1α-Puro (System Biosciences) by restriction digest with EcoRI and BamHI (pCMV-hHBEGF) ...
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bioRxiv - Neuroscience 2022Quote: ... dual-reporter lentiviral plasmids pLL410_EF1a-rFLuc-T2A-GFP-mPGK-Puro (LL410PA-1) and pLL-CMV-rFLuc-T2A-GFP-mPGK-Puro (LL310PA-1) were obtained from System Bioscience. Lentiviral generation was carried out as previously described [11] ...
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bioRxiv - Neuroscience 2022Quote: ... HEK 293T cells cultured in 10-cm Petri dishes at a 50-60% confluence were co-transfected with 10 μg lentiviral plasmid expressing miR-124-1 precursor under the CMV promoter and GFP under the EF1 promoter (SBI System Biosciences) or 10 μg lentiviral plasmid expressing GFP under the CMV promoter and the packaging plasmids pVSV-G (3.5 μg) ...
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bioRxiv - Developmental Biology 2019Quote: ... the PR-deletion coding sequences or the NLS-3xFlag was digested from their pCAGIG plasmids and inserted sequentially to the pCDH-CMV-MCS-EF1-Puro plasmid (System Biosciences) between the EcoRI and NotI sites (for Prdm16-FL and Prdm16-PRdeletion ...
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bioRxiv - Microbiology 2021Quote: For cDNA expression of WIPI2B proteins cells were transduced with lentivirus carrying the gene of interest with an N-terminal 3X Ty1 tag (Table S7) on the pCDH-CMV-MCS-T2A-Puro backbone (CD522A-1, System Biosciences). cDNA expression of ATG proteins has been previously described (21 ...
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bioRxiv - Immunology 2021Quote: ... Construct expressing lipin-1-mCherry was amplified by PCR adding 5’Xba-I and Sal-I 3’specific restriction sites and inserted into lentiviral vector pCDH-CMV-MCS-EF1α-copGFP (System Biosciences) after removing an internal Sal-I restriction site by mutagenesis ...
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bioRxiv - Bioengineering 2022Quote: ... were generated by transfection of a PiggyBac vector carrying a CMV promoter-driven firefly luciferase reporter gene with a super PiggyBac transposase expression vector (System Biosciences) 93 ...
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bioRxiv - Developmental Biology 2024Quote: ... with the primers 5’-GGGGAATTCGCCACCATGGGTTCTCA-3’ and 5’-CCC GCGGCCGCTCACTTCGCTGTCATCA-3’ was subcloned into EcoRI and NotI sites in the P B-CMV-MCS-EF1α-Puro PiggyBac transposon vector (PB510B-1, System Bioscience).
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bioRxiv - Cell Biology 2024Quote: ... PAAKRVKLD) from pCDH-NLSSV40-sfCherry-NLSMyc-Blast (30) were ligated to pCDH-CMV-MCS-EF1-Hygro (plasmid #CD515B-1, System Biosciences) using the Ligation-Convenience Kit (Nippon Gene ...
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bioRxiv - Cell Biology 2021Quote: ... Not1 digested and Calf Intestinal Phosphatase treated pCDH-CMV-MCS-EF1α-copGFP Dual Promoter Cloning and Expression Lentivector (System Biosciences LLC, #CD511B-1). Similarly ...
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bioRxiv - Cancer Biology 2021Quote: ... was constructed by inserting human HAUSP-WT or HAUSP-CS ORF cDNA into the pCDH-CMV-MCS-EF1a-Puro vector (System Biosciences, CD510B-1). The lentiviral plasmid expressing SOX2 was constructed by cloning human SOX2 ORF cDNA into the pCDH-CMV-MCS-EF1a-Puro vector (System Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... The lentiviral plasmid expressing SOX2 was constructed by cloning human SOX2 ORF cDNA into the pCDH-CMV-MCS-EF1a-Puro vector (System Biosciences, CD510B-1) or PCW57.1 vector (Addgene ...
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bioRxiv - Synthetic Biology 2020Quote: The plasmids containing the rTetR-effector fusions were cloned into the PB-CMV-MCS-EF1α-Puro PiggyBac vector backbone (System Biosciences #PB510B-1), which was further modified via Gibson Assembly with the following components ...
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bioRxiv - Molecular Biology 2023Quote: ... a minimal piggyBac cargo construct was created by deleting all intervening sequences between the 5’ and 3’ terminal repeats (including core insulators) of the PB-CMV-MCS-EF1α-Puro vector (System Biosciences, PB510B-1). A gBlock (Integrated DNA Technologies ...
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bioRxiv - Genomics 2023Quote: ... Fragments were resuspended at a final concentration of 100ng/ul in EB buffer and then cloned into a PB-CMV-MCS-EF1α-Puro PiggyBac cDNA Cloning and Expression Vector (PB vector hereafter, System Biosciences #PB510B-1). Following ligation ...