Labshake search
Citations for System Biosciences :
1 - 50 of 241 citations for Recombinant Mouse Tissue Inhibitor Of Metalloproteinase 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... The open reading frame from of murine XPR1 was tagged with a sequence for the HA-epitope and cloned into PiggyBac expression vector PB510B-1 (System Biosciences) with XbaI and NotI ...
-
bioRxiv - Cancer Biology 2023Quote: ... omentum tissues were minced with sterile razor blades and approximately 0.5 g of minced tissue was cultured per 100 mm tissue culture dish in 10 mL DMEM/F12 media supplemented with 1% exosome-depleted FBS (EXO-FBS-250A-1, System Biosciences, Palo Alto, CA). The following day ...
-
bioRxiv - Cell Biology 2022Quote: For stable expression of mScarlet (mSc) tagged IFT-A proteins (IFT144, IFT140, IFT122, IFT121, and IFT139) and associated mutants using the Super PiggyBac system (System Biosciences), the relevant open reading frames were inserted into the PB-EF1α-MCS-IRES-Neo vector with C-terminal TEV ...
-
bioRxiv - Cell Biology 2024Quote: Stable cell lines expressing GFP-tagged cytoplasmic tail chimeras under a cumate-inducible promoter were generated by PiggyBac transposition (System Biosciences). Wild-type ...
-
bioRxiv - Molecular Biology 2019Quote: HeLa cervix carcinoma cells (American Tissue Culture Collection: Manassas, VA, USA) and human embryonic kidney (HEK) 293TN cells (System Biosciences, LV900A-1) were cultured using Dulbecco’ s Modified Eagle Medium (DMEM ...
-
bioRxiv - Cancer Biology 2020Quote: Tissues were stained with antibody for anti-CD63 (System Bioscience, Mountain View, CA, USA). The secondary antibody ...
-
bioRxiv - Cancer Biology 2023Quote: EVs were isolated from 2.5 mL of 48 h conditioned media from tissue slice explants using ExoQuick-TC (System Biosciences, Palo Alto, CA, USA) per the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2019Quote: Mouse G3BP1 cDNAs were subcloned into pCDH-MCS-T2A-puro-MSCV lentiviral vector (System Biosciences) by NEBuilder HiFi DNA assembly (New England Biolabs) ...
-
bioRxiv - Cancer Biology 2023Quote: Mouse SIX4 cDNA (Genomics-online) was cloned into pCDH-CMV-MCS-EF1α-Puro Lentivector (System Biosciences). Human SIX4 cDNA was amplified from genomic DNA by PCR and cloned into pCDH-CMV-MCS-EF1α-Puro vector.
-
bioRxiv - Molecular Biology 2021Quote: ... with 1/5 final dilution (System Biosciences, LV810A-1), and the mixture was incubated at 4 °C for 6 hrs ...
-
bioRxiv - Molecular Biology 2019Quote: ... with 1 μg of transposase plasmid (System Biosciences #PB210PA-1) under conditions described above ...
-
bioRxiv - Microbiology 2020Quote: EV isolation from mouse serum was performed using ExoQuick™ solution (System Biosciences, Palo Alto, CA, USA). Following serum collection ...
-
bioRxiv - Cell Biology 2022Quote: ... a lentivirus expression plasmid (MMIR-1a-1-PA-1 and MMIR-206-PA-1 purchased from System Biosciences) and plasmid mixture for packaging (ViraPower HiPerform Lentiviral Expression Systems ...
-
bioRxiv - Biochemistry 2020Quote: ... anti-TSG101 antibody (EXOAB-TSG101-1; System Biosciences, CA; 1:500), anti-CD63 antibody (EXOAB-CD63A-1 ...
-
bioRxiv - Biochemistry 2020Quote: ... anti-CD63 antibody (EXOAB-CD63A-1; System Biosciences, CA; 1:500), anti-CD5L antibody (AF2834 ...
-
bioRxiv - Cell Biology 2021Quote: ... AP100R-1 (System Biosciences) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... Lentivector-based scrambled miRZip-000 (MZIP000-PA-1) and miRZip-146a (anti-miRNA; MZIP146a-PA-1) in S1505A-1 construct vector were purchased from System Biosciences. Both Scrambled and miRZIP vectors contained copGFP as a fluorescent reporter ...
-
bioRxiv - Cancer Biology 2022Quote: ... Both human and mouse BGN were subcloned into the PB-CMV-MCS-EF1α-Puro piggybac expression vector (System Biosciences) using the 5’ EcoRI and 3’ BamHI restriction sites ...
-
bioRxiv - Cancer Biology 2024Quote: cDNA of mouse Dnmt3A1WT and DnmtA1W326R (corresponding to human W330R) previously cloned into pCDH-EF1-MCS-Neo (System Biosciences) with an N-terminal FLAG-HA epitope tag(37 ...
-
bioRxiv - Cancer Biology 2023Quote: ... along with two housekeeping genes (Supplementary Table 1; CAS9-PR-1 from System Biosciences). The Kcnn4 and Cas9 mRNA levels for each sample were measured in technical duplicates for each primer set ...
-
bioRxiv - Developmental Biology 2020Quote: Human SC102A-1 (System Bioscience) and chimpanzee Sandra A iPSC lines (Camp et al. ...
-
bioRxiv - Microbiology 2022Quote: HEK293T (System Biosciences, LV900A-1) and HEK293-Spike cells (SARS-CoV-2 Spike (D614)-expressing 293 cells [293-SARS2-S cells ...
-
bioRxiv - Developmental Biology 2020Quote: ... DLD-1 cells were co-transfected with a PiggyBac transposase vector (System Biosciences, PB210PA-1) and a PiggyBac-ABA-CRISPRi vector (25 ...
-
bioRxiv - Cell Biology 2023Quote: ... 1 million cells were co-transfected with 1 µg ePiggyBac transposase expression plasmid (System Biosciences) and 1 µg each epB-MCP-mScarletI ...
-
bioRxiv - Developmental Biology 2023Quote: ... or goat anti-rabbit (1:20,000; EXOAB-KIT-1; System Biosciences, Palo Alto, CA, USA). Protein signals were detected using the SuperSignal West Femto Chemiluminescent kit (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2023Quote: ... and incubated overnight at 4°C with 1:1000 primary antibody (CD63 and CD81, rabbit anti-human, EXOAB-CD63A-1/ EXOAB-CD81A-1, System Biosciences, Palo Alto, CA, USA) in TBST ...
-
bioRxiv - Molecular Biology 2019Quote: HEK293TN cells (System Biosciences, LV900A-1) and HeLA cells (American Tissue Culture Collection ...
-
bioRxiv - Molecular Biology 2019Quote: HEK293TN cells (System Biosciences, LV900A-1) were cultured using Dulbecco’s Modified Eagle Medium (DMEM ...
-
bioRxiv - Genomics 2022Quote: THP-1 cells were transduced in triplicates using the TransDux MAX transfection reagent (System Biosciences, LV860A-1) according to the manufacturer’s recommendations ...
-
bioRxiv - Genetics 2021Quote: ... along with 1/10 total plasmid mass of Super PiggyBac Transposase Expression Vector (System Bioscience, Inc. #PB210PA-1). Stable integrants were selected with 1-2 μg/mL puromycin.
-
bioRxiv - Cell Biology 2022Quote: ... Stock cumate solution (System Biosciences # QM100A-1) was diluted to 5X in culture medium prior to use.
-
bioRxiv - Cell Biology 2021Quote: ... with piggyBac transposase (PB210PA-1, System Biosciences) and a piggyBac plasmid expressing SpCas9 under a doxycycline inducible promoter and containing a blasticidin selection cassette ...
-
bioRxiv - Immunology 2021Quote: IL-23 EEV(EEV651A-1, System Biosciences) was hydrodynamically injected in a volume of sterile HBSS (Invitrogen ...
-
bioRxiv - Cell Biology 2023Quote: ... and HEK293TN cells (System Biosciences, #LV900A-1) were routinely cultured in Dulbecco’s Modified Eagle Medium (DMEM ...
-
bioRxiv - Molecular Biology 2023Quote: ... ni piggyBac transposase (PB200PA-1; System Biosciences) (2) ...
-
bioRxiv - Microbiology 2023Quote: ... and HR110PA-1 (System Biosciences, CA, USA). The pX330-puro vector was constructed by integrating the puromycin resistance gene sequence from the HR110PA-1 vector into the pX330 vector ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 0.5% TransDux (LV860A-1, System Biosciences). 72 hours after transduction ...
-
bioRxiv - Neuroscience 2020Quote: ... and the membrane and intra-vesicular positive EV markers CD81 (1:500; cat. no. EXOAB-CD81A-1, System Biosciences) and alix (0.4 μg/mL ...
-
bioRxiv - Cell Biology 2020Quote: ... or a pCDH vector (System Biosciences, CD550A-1). JNK1 (NCBI RefSeq NM_001323302.1 ...
-
bioRxiv - Neuroscience 2022Quote: ... HEK293TN cells (System Biosciences, cat. no. LV900A-1) and HeLa cells (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH ...
-
bioRxiv - Bioengineering 2021Quote: ... coli Minicircle Strain (System Biosciences, catalog #: MN900A-1) overnight at 37°C ...
-
bioRxiv - Cancer Biology 2022Quote: ... pGreenpuro shRNA cloning and expression lentivector was purchased from System Bioscience (Cat. No. SI505A-1, System Biosciences, LLC, CA). Primers for shRNA are shown in S1 Table ...
-
bioRxiv - Developmental Biology 2022Quote: ... using Cold Fusion cloning (System Biosciences, MC010B-1). All constructs were sequence verified for successful insert integration ...
-
bioRxiv - Genetics 2022Quote: ... and concentrated using PEG (System Biosciences, LV825A-1) following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... dual-reporter lentiviral plasmids pLL410_EF1a-rFLuc-T2A-GFP-mPGK-Puro (LL410PA-1) and pLL-CMV-rFLuc-T2A-GFP-mPGK-Puro (LL310PA-1) were obtained from System Bioscience. Lentiviral generation was carried out as previously described [11] ...
-
bioRxiv - Developmental Biology 2020Quote: ... 7 that exhibited distinct spots by transient expression (Supplementary table 1) were subcloned into a piggybac-vector PB510B-1 (System BioSciences) to establish stable lines ...
-
bioRxiv - Cell Biology 2020Quote: Lentiviral cloning and expression vectors pCDH-UbC-MCS-EF1-Hygro (CD615B-1) and pCDH-EF1-MCS-T2A-Puro (CD520A-1) were purchased from System Biosciences Inc ...
-
bioRxiv - Immunology 2020Quote: To silence miR-424 the GFP tagged lentiviral vectors that express antisense hsa-miR-424 (MZIP424-PA-1) and corresponding scramble control lentiviral vectors (MZIP000-PA-1) were purchased from SBI (System Biosciences). Transfection of 293T packaging cell line was performed using calcium-phosphate protocol and infection of target cells was done as previously described (Platzer et al. ...
-
bioRxiv - Molecular Biology 2021Quote: ... for 1 h at room temperature and incubated with primary antibodies overnight at 4 °C with anti-Cd9 ([1:3000; System Biosciences, Palo Alto ...
-
bioRxiv - Developmental Biology 2020Quote: ... 4 mL 5× PEG reagent (System Biosciences, LV825A-1) was added and mixed by pipetting ...