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Citations for System Biosciences :
101 - 150 of 296 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... dual-reporter lentiviral plasmids pLL410_EF1a-rFLuc-T2A-GFP-mPGK-Puro (LL410PA-1) and pLL-CMV-rFLuc-T2A-GFP-mPGK-Puro (LL310PA-1) were obtained from System Bioscience. Lentiviral generation was carried out as previously described [11] ...
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bioRxiv - Developmental Biology 2020Quote: ... 7 that exhibited distinct spots by transient expression (Supplementary table 1) were subcloned into a piggybac-vector PB510B-1 (System BioSciences) to establish stable lines ...
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bioRxiv - Cell Biology 2020Quote: Lentiviral cloning and expression vectors pCDH-UbC-MCS-EF1-Hygro (CD615B-1) and pCDH-EF1-MCS-T2A-Puro (CD520A-1) were purchased from System Biosciences Inc ...
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bioRxiv - Immunology 2020Quote: To silence miR-424 the GFP tagged lentiviral vectors that express antisense hsa-miR-424 (MZIP424-PA-1) and corresponding scramble control lentiviral vectors (MZIP000-PA-1) were purchased from SBI (System Biosciences). Transfection of 293T packaging cell line was performed using calcium-phosphate protocol and infection of target cells was done as previously described (Platzer et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... for 1 h at room temperature and incubated with primary antibodies overnight at 4 °C with anti-Cd9 ([1:3000; System Biosciences, Palo Alto ...
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bioRxiv - Cell Biology 2024Quote: ... pseudoviral particles were produced using 293TN cells (System Biosciences, SBI). The 293TN cells were transfected with the lentiviral plasmid of interest as well as lentiviral packaging and envelope plasmids (psPAX and pMD2.G ...
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bioRxiv - Developmental Biology 2022Quote: ... with 250 μg protein equivalent of AFSC-EVs stained using 2 μL of ExoGlow™-Vivo (Near IR) EV Labeling Kit (System Biosciences, Palo Alto, CA).
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bioRxiv - Developmental Biology 2020Quote: ... 4 mL 5× PEG reagent (System Biosciences, LV825A-1) was added and mixed by pipetting ...
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bioRxiv - Biophysics 2022Quote: ... PB-CMV-MCS-EF1α-Puro (System Bioscience #PB510B-1) was linearized using EcoRI and BamHI ...
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bioRxiv - Cell Biology 2021Quote: ... following manufacturer instructions (QuantiMir, System Biosciences, cat. RA670A-1). To acquire the miRNA-enriched RNA fraction from adipose tissue miRNeasy Mini Kit was used (Qiagen ...
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bioRxiv - Cancer Biology 2022Quote: ... and precipitated with PEG-it (#LV825A-1, System Biosciences). The virus precipitates were resuspended in PBS and stored at −80°C ...
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bioRxiv - Neuroscience 2022Quote: The clonal control iPSC lines SC102A-1 (System Biosciences) and CRTDi011-A (this study) ...
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bioRxiv - Physiology 2023Quote: ... ExoQuick (Cat #EXOQ5A-1, System Biosciences, Palo Alto, CA) was added to the supernatant at a 1:4 ratio for 30 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... TransDux MAX Lentivirus Transduction Enhancer (System Biosciences LV860A-1), and 10 uM Y-27632 and plated as monolayers on Collagen I (Advanced Biomatrix 5010) ...
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bioRxiv - Cancer Biology 2024Quote: ... A water-soluble cumate solution (QM150A-1, System Biosciences) was used to induce PAX3::FOXO1-T2A-mCherry ...
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bioRxiv - Cancer Biology 2021Quote: ... the medium was replaced with DMEM without FBS for 1 hour before the plasmids pCDH-GAL-9-T2A-Puro (System Biosciences, CD527A-1) or pLKO ...
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bioRxiv - Genomics 2023Quote: ... The lentivirus-containing media was mixed at a 1:4 ratio of PEG-it virus precipitation solution (System Biosciences, Cat. No. LV810A-1) to media ...
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bioRxiv - Synthetic Biology 2021Quote: ... A total of 4 μg of piggyBac targeting base editor plasmids were transfected with 1 μg of super transposase plasmid (SBI System Biosciences cat# PB210PA-1) using Lipofectamine 3000 following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... omentum tissues were minced with sterile razor blades and approximately 0.5 g of minced tissue was cultured per 100 mm tissue culture dish in 10 mL DMEM/F12 media supplemented with 1% exosome-depleted FBS (EXO-FBS-250A-1, System Biosciences, Palo Alto, CA). The following day ...
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bioRxiv - Genetics 2021Quote: ... This plasmid was derived from the MN530A-1 (System Biosciences) by replacing the GFP Reporter sequence with a multiple cloning site using the restriction enzymes XmaI and StuI and duplexed donor oligonucleotides #2020 and #2021 (Supplemental Table 1) ...
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bioRxiv - Immunology 2021Quote: ... 55 mL of PureFection reagent (System Biosciences; Cat # LV750A-1) was added to each 1mL tube ...
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bioRxiv - Bioengineering 2022Quote: ... 0.5μg of Super PiggyBac Transposase (System Biosciences, PB210PA-1-SBI) and 0.5μg of piRFP670-N1 (addgene #45457 ...
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bioRxiv - Cell Biology 2022Quote: ... with a PiggyBac transposon expression vector (PB210PA-1; System Biosciences), or pEGFP-C1-FLAG-Ku80 ((Britton et al. ...
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bioRxiv - Synthetic Biology 2022Quote: ... or PiggyBac Transposon vector plasmid (System Biosciences, Cat # PB510B-1). Plasmids encoding 2nd-generation packaging plasmids (psPAX2 – Cat #12260 ...
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bioRxiv - Physiology 2023Quote: ... and transfected with CD81-GFP (System Biosciences # CYTO124-PA-1) and mPlum-mito-3 (Addgene plasmid #55988 ...
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bioRxiv - Neuroscience 2022Quote: ... The Super PiggyBac Transposase plasmid (System Biosciences; Cat# PB210PA-1) was obtained from BioCat ...
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bioRxiv - Biochemistry 2023Quote: The PiggyBac Single Promotor vector (cat# PB531A-1, System Biosciences) was modified in house to replace the gene encoding the red fluorescent protein (RFP ...
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bioRxiv - Neuroscience 2023Quote: ... into PB-CMV-MCS-EF1-Puro (System Biosciences #PB510B-1).
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bioRxiv - Neuroscience 2023Quote: ... into PB-CMV-MCS-EF1-Puro (System Biosciences #PB510B-1).
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bioRxiv - Neuroscience 2023Quote: ... into PB-CMV-MCS-EF1-Puro (System Biosciences #PB510B-1).
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bioRxiv - Genetics 2023Quote: ... Super PiggyBac Transposase (PB210PA-1) was purchased from System Biosciences.
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bioRxiv - Cancer Biology 2024Quote: ... 20% TransDux MAX Lentivirus Transduction Enhancer (LV860A-1, System Biosciences), and 0.5% TransDux (LV860A-1 ...
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bioRxiv - Cell Biology 2021Quote: ... cells were cultured in EV-depleted FBS (System Biosciences, Palo Alto, CA). Plates (14 ...
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bioRxiv - Synthetic Biology 2023Quote: ... HEK293FT cells were transfected with the vector and transposon plasmid (System Biosciences). Transfected Cells were incubated for 72 hours before selection with media containing 2 ug/mL Puromycin (Invivogen ant-pr-1) ...
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bioRxiv - Molecular Biology 2020Quote: ... exosome-depleted FBS (cat. no. EXO-FBS-50A-1, System Biosciences) was added to the cells ...
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bioRxiv - Biophysics 2021Quote: ... and mwGFP was induced by 1x cumate (QM100A-1, System Biosciences) 24-48h before imaging ...
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bioRxiv - Microbiology 2021Quote: ... a lentiviral vector pCDH-MSCV-T2A-Puro (System Biosciences, #CD522A-1) was modified to enable zeocin selection instead of puromycin ...
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bioRxiv - Genomics 2022Quote: ... shRNA templates were cloned into pGreenPuro lentivector (System Biosciences, SI505A-1). shRNA targets are ...
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bioRxiv - Cell Biology 2022Quote: ... and the Super PiggyBac Transposase Expression Vector (System Biosciences, PB210PA-1) using Lipofectamine 3000 Transfection Reagent (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... 5x PEG-it Virus Precipitation Solution (System Biosciences, Cat # LV810A-1) was added to supernatants and incubated 4°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... 2.5 ml of sterile PEG-it solution (System Biosciences, #LV810A-1) was added to 10 ml of filtered viral supernatant and stored at 4°C for 48 hrs ...
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bioRxiv - Neuroscience 2022Quote: ... streptavidin magnetic Exo-Flow beads (System Biosciences, Inc.; Catalog # CSFLOWBASICA-1) were incubated with 100 ng/μL of mouse anti-human CD171 (L1CAM ...
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bioRxiv - Bioengineering 2023Quote: ... mRNA constructs were transferred to the FC550A-1 backbone (System Biosciences) belonging to their ϕC31 integrase vector system using the same restriction sites.
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bioRxiv - Cell Biology 2023Quote: ... the media was supplemented with 1X cumate (System Biosciences QM159A-1) to induce expression of the rescue constructs ...
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bioRxiv - Microbiology 2021Quote: ... tHF cells stably transduced with the IFN-responsive pGreenFire-ISRE lentivector (System Biosciences) were further stably transduced with the lentiCRISPRv2 vector ...
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bioRxiv - Cancer Biology 2020Quote: SW480 CRC cells were transduced with lentivirus carrying pCDH vector from System Biosciences: empty vector (Mock ...
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bioRxiv - Biochemistry 2020Quote: ... and a lentiviral transfer vector CD512-EF1a-RFP (System Biosciences CD512B-1) using Lipofectamine 2000 ...
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bioRxiv - Cancer Biology 2021Quote: ... and pLL-EF1a-rFLuc-T2A-GFP-mPGK-Puro (LL410PA-1, System Biosciences). Preparation and transduction was completed as previously described,(25 ...
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bioRxiv - Biochemistry 2021Quote: ... and a lentiviral transfer vector CD512-EF1a-RFP (System Biosciences CD512B-1) using Lipofectamine 2000 ...
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bioRxiv - Immunology 2020Quote: ... These constructs were cloned into the episomal vector EEV600A-1 (System Biosciences) into which a puromycin resistance cassette was added so that cell pools expressing the proteins could be rapidly selected ...