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Citations for System Biosciences :
1 - 50 of 90 citations for Mouse WD repeat containing protein WRAP73 WRAP73 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... a minimal piggyBac cargo construct was created by deleting all intervening sequences between the 5’ and 3’ terminal repeats (including core insulators) of the PB-CMV-MCS-EF1α-Puro vector (System Biosciences, PB510B-1). A gBlock (Integrated DNA Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... EVs were isolated from tissue culture media using a commercial kit containing a polyethylene glycol (PEG) solution (ExoQuick-TC ULTRA kit, EQULTRA-20TC-1; System Biosciences) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... exosomes from ASCs were labeled fluorescent red using an ExoGlow-Protein EV Labeling Kit (System Biosciences) and then incubated with precipitation solution at 4°C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... The number of CD63+ EVs collected in the EV-enriched fractions were estimated by ELISA (System Biosciences). The ELISA standards provided in the kit are calibrated by NTA to measure the number of exosomes and establish a standard curve based on exosome abundance ...
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bioRxiv - Synthetic Biology 2020Quote: ... Exo-Check Array: A549 protein markers were characterised using a commercially sourced Exo-Check Exosome Antibody Array kit (#EXORAY200A-4, System Biosciences, CA, USA). A549 EVs (100 μg ...
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bioRxiv - Molecular Biology 2019Quote: The identification of protein markers on the isolated exosomes was undertaken using the commercially available Exo-Check Exosome Ab Array kit (System Biosciences, Palo Alto, CA), as described by the manufacturer ...
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bioRxiv - Cancer Biology 2022Quote: ... a plasmid containing the truncated ATR cDNA (containing the first 779 codons only) and a Kanamycin resistance marker was obtained (System Biosciences, SBI, Palo Alto, CA). This plasmid was built into SBI’s FC500A-1 vector that contains attB sites which allow the target sequence to be stably integrated into mammalian genomes in low copy number when co-transfected with ϕC31 integrase ...
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bioRxiv - Developmental Biology 2022Quote: ... with 250 μg protein equivalent of AFSC-EVs stained using 2 μL of ExoGlow™-Vivo (Near IR) EV Labeling Kit (System Biosciences, Palo Alto, CA).
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bioRxiv - Genomics 2019Quote: ... Cas9 protein was bought from System Biosciences (2438 Embarcadero Way ...
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bioRxiv - Immunology 2020Quote: ... Supernatant containing viral particles was collected and concentrated using PEG-IT (System Biosciences) followed by centrifugation at 2600 rpm ...
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bioRxiv - Developmental Biology 2021Quote: ... 8 pl of a mixture containing 500 ng/μl RFP-Piranha mRNA (System Biosciences) and 0.1 mg/ml anti-Tdrd3 antibody (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2021Quote: ... A lentiviral plasmid containing guide RNA sequences was transfected into 293TN cells (System Biosciences) using Lipofectamine 2000 transfection reagent ...
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bioRxiv - Cancer Biology 2022Quote: ... Media containing virus particles was mixed with PEG-it virus precipitation solution (System Biosciences, #LV810A-1) and incubated at 4°C overnight ...
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bioRxiv - Synthetic Biology 2020Quote: ... The human proteins were assigned to 3 Piggybac vectors (System Biosciences, USA) by Golden Gate Assembly method (11 ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lines were immediately cryopreserved and maintained in selection medium containing 60 μg/mL cumate (System Biosciences) for at least one passage prior to assay.
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviruses containing the luciferase expression cassette were produced in 293 TN cells (System Bioscience, catalog number: LV900A-1) by transient transfection with the PSMPUW-Hygro lentiviral vector carrying the luciferase gene and the ViraSafe™ Lentiviral Packaging System (Cell Biolabs ...
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bioRxiv - Cancer Biology 2021Quote: ... This supernatant containing lentiviral particles was concentrated using PEGit virus precipitation solution according to the manufacturer’s protocol (System Biosciences). JAM-A knockdown constructs were as follows ...
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bioRxiv - Cancer Biology 2022Quote: ... supernatant containing lentiviral particles was collected and concentrated with PEGit virus precipitation solution according to manufacturer’s protocol (System Biosciences). CSCs were plated on Geltrex ...
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bioRxiv - Neuroscience 2022Quote: ... supernatant containing eluted exosomes were incubated with 1 μL of Exo-FlowIP clearing reagent (System Biosciences, Inc.; Catalog # EXOFLOW32A) at 37°C for 30 min then stored at −80°C.
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bioRxiv - Developmental Biology 2022Quote: ... The gRNA (5 pg/embryo) and mRNA encoding Cas9 protein (150 pg/embryo, System Biosciences) were injected into one-cell stage embryos ...
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bioRxiv - Genetics 2019Quote: To produce lentiviral cell lines containing pGreenFire1™ Pathway Reporter lentivector (Cat#TR010PA-N and Cat#TR000PA-1, System Bioscience) expressing the MUC5B promoter region and controls ...
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bioRxiv - Microbiology 2019Quote: Mouse G3BP1 cDNAs were subcloned into pCDH-MCS-T2A-puro-MSCV lentiviral vector (System Biosciences) by NEBuilder HiFi DNA assembly (New England Biolabs) ...
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bioRxiv - Immunology 2021Quote: ... 5’ ATACGCGGATCCtcagtagggccgccacac 3’ and cloned into a commercial lentivector containing a GFP reporter gene (pCDH-GFP-Em-CD513B-1 from System Biosciences). To overexpress RUNX2 in human T cells ...
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bioRxiv - Cell Biology 2020Quote: ... with the respective lamin containing or empty (Mock) vectors into HEK 293-TN cells using PureFection nanotechnology-based transfection reagent (System Biosciences). The medium was exchanged the next day and replaced by antibiotics-free DMEM containing 10% FCS ...
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bioRxiv - Cell Biology 2022Quote: ... Culture medium containing the shed virus was collected at 72 hours and incubated with PEG-it virus precipitation solution (System Biosciences) at 4°C for 48 hours ...
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bioRxiv - Bioengineering 2023Quote: ... The in vivo T cell activation reporter was constructed by cloning a sequence containing firefly luciferase into the pGreenFire1-NF-κB lentiviral vector (System Biosciences) under the NF-κB responsive promoter ...
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bioRxiv - Cancer Biology 2023Quote: Mouse SIX4 cDNA (Genomics-online) was cloned into pCDH-CMV-MCS-EF1α-Puro Lentivector (System Biosciences). Human SIX4 cDNA was amplified from genomic DNA by PCR and cloned into pCDH-CMV-MCS-EF1α-Puro vector.
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bioRxiv - Microbiology 2022Quote: ... using the Multiplex gRNA kit (System Biosciences) according to the manufacturer protocol ...
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bioRxiv - Microbiology 2020Quote: EV isolation from mouse serum was performed using ExoQuick™ solution (System Biosciences, Palo Alto, CA, USA). Following serum collection ...
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bioRxiv - Synthetic Biology 2020Quote: The plasmids containing the rTetR-effector fusions were cloned into the PB-CMV-MCS-EF1α-Puro PiggyBac vector backbone (System Biosciences #PB510B-1), which was further modified via Gibson Assembly with the following components ...
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bioRxiv - Genomics 2023Quote: ... The lentivirus-containing media was mixed at a 1:4 ratio of PEG-it virus precipitation solution (System Biosciences, Cat. No. LV810A-1) to media ...
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bioRxiv - Genetics 2023Quote: ... 6 X 105 cells were co-transfected with 5 µg of gRNA-containing piggyBac vector and 1 µg of piggyBac transposase plasmid (System Biosciences PB210PA-1) using Lipofectamine 3000 Transfection Reagent ...
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bioRxiv - Molecular Biology 2023Quote: ... using the Cold Fusion kit (System Biosciences Inc) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2020Quote: An amount of 4 ng of XMIRXpress vector containing Non-Targeting miRNA with Xmotif (cat. no. XMIRXP-NT, System Biosciences LLC, Palo Alto, CA, USA) was introduced into 100 μL Stellar competent E ...
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bioRxiv - Cancer Biology 2022Quote: ... Both human and mouse BGN were subcloned into the PB-CMV-MCS-EF1α-Puro piggybac expression vector (System Biosciences) using the 5’ EcoRI and 3’ BamHI restriction sites ...
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bioRxiv - Cancer Biology 2024Quote: cDNA of mouse Dnmt3A1WT and DnmtA1W326R (corresponding to human W330R) previously cloned into pCDH-EF1-MCS-Neo (System Biosciences) with an N-terminal FLAG-HA epitope tag(37 ...
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bioRxiv - Bioengineering 2023Quote: ... and the pPACKH1 HIV Lentivector Packaging Kit (System Biosciences) was used to produce lentivirus particles ...
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bioRxiv - Cell Biology 2021Quote: ... Virus-containing supernatants were either collected or concentrated by mixing with quarter volumes of 5 × PEG-it Virus Precipitation Solution (System Biosciences, Palo Alto, CA, United States), followed by centrifugation according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Double-stranded oligonucleotides were cloned into the linearized SmartNuclease vector (EF1-T7-hspCas9-T2A-RFP-H1-gRNA with red fluorescent protein (RFP, System Biosciences) and IFNLR1-guide RNA (IFNLR1-gRNA ...
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bioRxiv - Cell Biology 2022Quote: For stable expression of mScarlet (mSc) tagged IFT-A proteins (IFT144, IFT140, IFT122, IFT121, and IFT139) and associated mutants using the Super PiggyBac system (System Biosciences), the relevant open reading frames were inserted into the PB-EF1α-MCS-IRES-Neo vector with C-terminal TEV ...
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bioRxiv - Synthetic Biology 2020Quote: ... and then transfected with three piggyBac vectors expressing multiple human proteins with the Neon Transfection System with the presence of piggyBac Transposase (System Biosciences, USA). Two days after ...
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bioRxiv - Molecular Biology 2022Quote: ... Colonies were stained using an Alkaline Phosphatase Assay Kit (System Biosciences) and evaluated under an Olympus CK2 microscope.
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bioRxiv - Neuroscience 2021Quote: ... Exosomes were extracted from supernatant with an ExoQuick kit (System Biosciences). The presence of exosomal markers and size distribution of the particles was confirmed previously [22] ...
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bioRxiv - Neuroscience 2020Quote: ... fibrinogen and fibrin proteins known to impede efficient EV recovery were removed using 5 U/mL of thrombin (System Biosciences, Inc.; Mountainview, CA) for 30 minutes at RT followed by addition of 495 μL of Dulbecco’s PBS-1X (DPBS ...
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bioRxiv - Biochemistry 2021Quote: ... or ExoQuick ULTRA EV Isolation Kit for Serum and Plasma (System Biosciences), and protocols were performed according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Microvesicles were isolated using ExoQuick-TC kit (System Biosciences, Palo Alto, USA) accordingly to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... samples were purified using the CD9 Exo-Flow Capture Kit (System Biosciences) using the manufacturer-provided protocol ...
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bioRxiv - Developmental Biology 2022Quote: AFSC-EVs were fluorescently labelled using ExoGlow-EV labeling kit (SBI System Biosciences), using the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... concentrated by PEG-it™ precipitation kits from System Bioscience (Palo Alto, CA), were re-suspended in cold PBS and stored at −80 °C.
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bioRxiv - Developmental Biology 2022Quote: ... pre-digested with XhoI and HindIII using Cold Fusion Cloning kit (System Biosciences). Deletion of putative TF binding sites from those enhancers were performed using QuickChange II XL site-directed mutagenesis kit (Agilent Technologies) ...