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Citations for System Biosciences :
1 - 50 of 286 citations for Mouse Oxidation resistance protein 1 Oxr1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... pT-mAppleT2Apuro was generated by PCR amplifying the T2A peptide and puromycin resistance gene from PB-CMV-MCS-GreenPuro (System Biosciences, Cat# PB513B-1) and cloning into pT-mApple (Vectorbuilder) ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA fragments containing the kanamycin-resistance gene (kmr) were amplified from a MCS1-EF1α-Blasticidin-pA-MCS2-PGK-hsvTK HR targeting vector (System Biosciences, USA). The overlapping oligonucleotide primers were designed by NEBuilder Assembly Tool (New England Biolabs ...
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bioRxiv - Cancer Biology 2022Quote: ... a plasmid containing the truncated ATR cDNA (containing the first 779 codons only) and a Kanamycin resistance marker was obtained (System Biosciences, SBI, Palo Alto, CA). This plasmid was built into SBI’s FC500A-1 vector that contains attB sites which allow the target sequence to be stably integrated into mammalian genomes in low copy number when co-transfected with ϕC31 integrase ...
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bioRxiv - Molecular Biology 2021Quote: ... exosomes from ASCs were labeled fluorescent red using an ExoGlow-Protein EV Labeling Kit (System Biosciences) and then incubated with precipitation solution at 4°C overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... or goat anti-rabbit (1:20,000; EXOAB-KIT-1; System Biosciences, Palo Alto, CA, USA). Protein signals were detected using the SuperSignal West Femto Chemiluminescent kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... The number of CD63+ EVs collected in the EV-enriched fractions were estimated by ELISA (System Biosciences). The ELISA standards provided in the kit are calibrated by NTA to measure the number of exosomes and establish a standard curve based on exosome abundance ...
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bioRxiv - Molecular Biology 2021Quote: ... EVs were isolated from tissue culture media using a commercial kit containing a polyethylene glycol (PEG) solution (ExoQuick-TC ULTRA kit, EQULTRA-20TC-1; System Biosciences) according to the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2020Quote: ... Exo-Check Array: A549 protein markers were characterised using a commercially sourced Exo-Check Exosome Antibody Array kit (#EXORAY200A-4, System Biosciences, CA, USA). A549 EVs (100 μg ...
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bioRxiv - Molecular Biology 2019Quote: The identification of protein markers on the isolated exosomes was undertaken using the commercially available Exo-Check Exosome Ab Array kit (System Biosciences, Palo Alto, CA), as described by the manufacturer ...
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bioRxiv - Developmental Biology 2022Quote: ... with 250 μg protein equivalent of AFSC-EVs stained using 2 μL of ExoGlow™-Vivo (Near IR) EV Labeling Kit (System Biosciences, Palo Alto, CA).
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bioRxiv - Genomics 2019Quote: ... Cas9 protein was bought from System Biosciences (2438 Embarcadero Way ...
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bioRxiv - Microbiology 2020Quote: ... Fusion was performed with the Cold Fusion Cloning Kit (SBI System Biosciences, Cat. #MC010B-1).
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bioRxiv - Cell Biology 2021Quote: Inducible Cas9 iPSCs were generated using an AAVS1 TALE-Nuclease KIT (GE60xA-1; System Biosciences) and an iCas9 HR donor plasmid containing a TRE3G promoter-driven spCas9 with a C-terminal MYC NLS followed by a CMV promoter-driven Tet-On-3G and a neomycin resistance adapted from Ihry et al ...
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bioRxiv - Bioengineering 2023Quote: ... and anti-rabbit HRP-linked secondary antibody (EXOAB-KIT-1) were purchased from System Biosciences (CA, USA). Anti-beta-actin antibody (sc-47778) ...
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bioRxiv - Developmental Biology 2020Quote: ... ESC were washed twice with PBS and incubated with AP Staining kit™ (Cat # AP100R-1, System Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... was added to a tube along with purefection reagent and pPackF1 lentiviral packaging kit plasmids (System Biosciences, # LV100A-1) and either PCF1 (empty vector control) ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... Quantification of successfully integrated piggyBac transposons was performed using a piggyBac qPCR copy number kit (System Biosciences, PBC100A-1) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: RNA was isolated from the EVs using the Exoquick RNA column purification kit (EQ808A-1, System Biosciences, Palo Alto, CA, USA). Purified RNA was quantified using a Bioanalyzer and the Agilent RNA 6000 Pico kit (5067-1513 ...
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bioRxiv - Synthetic Biology 2020Quote: ... The human proteins were assigned to 3 Piggybac vectors (System Biosciences, USA) by Golden Gate Assembly method (11 ...
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bioRxiv - Cell Biology 2023Quote: ... The tag donor was also introduced as a minicircle generated with the MC-Easy Minicircle DNA Production Kit according to manufacturer’s instructions (System Biosciences, MN920A-1). Both solutions were combined ...
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bioRxiv - Cell Biology 2023Quote: ... The tag donor was also introduced as a minicircle generated with the MC-Easy Minicircle DNA Production Kit according to manufacturer’s instructions (System Biosciences, MN920A-1). Both solutions were combined ...
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bioRxiv - Physiology 2023Quote: Isolated Evs were processed for total RNA isolation using the SeraMir Exosome RNA purification kit (Cat #RA808A-1, System Biosciences, Palo Alto, CA), according to the manufacturer’s instructions provided with the kit ...
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bioRxiv - Developmental Biology 2022Quote: ... The amplified coding sequences followed by p2a-tdTomato were cloned downstream of the endothelial specific fli1a promotor into a Tol2 enabled vector using the Cold Fusion Cloning kit (System Biosciences, Cat# MC010B-1).
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bioRxiv - Cell Biology 2019Quote: ... the collected exosomes were further purified with anti-CD9 antibody-coated magnetic beads (CD9 Exo-Flow Exosome Purification Kit, System Biosciences, Cat. EXOTC10A-1) as in (Rim and Kim ...
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bioRxiv - Neuroscience 2022Quote: ... 0N4R tau was replaced with 2N4R tau after inserting 2N4R tau between BamHI and SalI restriction sites using a cold fusion cloning kit (System Biosciences, Catalog # MC100B-1). A Q5 Site-Directed Mutagenesis Kit (New England BioLabs ...
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bioRxiv - Bioengineering 2022Quote: ... EVs were also quantified based on the amount of total immunoreactive CD63 within isolated EV samples using the ExoELISA-ULTRA Complete Kit (System Biosciences, EXEL-ULTRA-CD63-1) per the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... The gRNA (5 pg/embryo) and mRNA encoding Cas9 protein (150 pg/embryo, System Biosciences) were injected into one-cell stage embryos ...
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bioRxiv - Microbiology 2019Quote: Mouse G3BP1 cDNAs were subcloned into pCDH-MCS-T2A-puro-MSCV lentiviral vector (System Biosciences) by NEBuilder HiFi DNA assembly (New England Biolabs) ...
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bioRxiv - Cell Biology 2020Quote: ... purification of EVs was performed in plasma by UC-I (bellow) and in conditioned medium according to one of the following protocols (Fig.1a):-(Exoquick) ExoQuick® commercial kit (System Biosciences EXOTC10A-1, Palo Alto, CA), following manufacturer’s instructions;
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bioRxiv - Cancer Biology 2023Quote: Mouse SIX4 cDNA (Genomics-online) was cloned into pCDH-CMV-MCS-EF1α-Puro Lentivector (System Biosciences). Human SIX4 cDNA was amplified from genomic DNA by PCR and cloned into pCDH-CMV-MCS-EF1α-Puro vector.
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bioRxiv - Molecular Biology 2021Quote: ... with 1/5 final dilution (System Biosciences, LV810A-1), and the mixture was incubated at 4 °C for 6 hrs ...
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bioRxiv - Microbiology 2022Quote: ... using the Multiplex gRNA kit (System Biosciences) according to the manufacturer protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... with 1 μg of transposase plasmid (System Biosciences #PB210PA-1) under conditions described above ...
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bioRxiv - Microbiology 2020Quote: EV isolation from mouse serum was performed using ExoQuick™ solution (System Biosciences, Palo Alto, CA, USA). Following serum collection ...
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bioRxiv - Cell Biology 2022Quote: ... a lentivirus expression plasmid (MMIR-1a-1-PA-1 and MMIR-206-PA-1 purchased from System Biosciences) and plasmid mixture for packaging (ViraPower HiPerform Lentiviral Expression Systems ...
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bioRxiv - Molecular Biology 2023Quote: ... using the Cold Fusion kit (System Biosciences Inc) according to manufacturer instructions ...
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bioRxiv - Biochemistry 2020Quote: ... anti-TSG101 antibody (EXOAB-TSG101-1; System Biosciences, CA; 1:500), anti-CD63 antibody (EXOAB-CD63A-1 ...
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bioRxiv - Biochemistry 2020Quote: ... anti-CD63 antibody (EXOAB-CD63A-1; System Biosciences, CA; 1:500), anti-CD5L antibody (AF2834 ...
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bioRxiv - Cell Biology 2021Quote: ... AP100R-1 (System Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Lentivector-based scrambled miRZip-000 (MZIP000-PA-1) and miRZip-146a (anti-miRNA; MZIP146a-PA-1) in S1505A-1 construct vector were purchased from System Biosciences. Both Scrambled and miRZIP vectors contained copGFP as a fluorescent reporter ...
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bioRxiv - Cancer Biology 2022Quote: ... Both human and mouse BGN were subcloned into the PB-CMV-MCS-EF1α-Puro piggybac expression vector (System Biosciences) using the 5’ EcoRI and 3’ BamHI restriction sites ...
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bioRxiv - Cancer Biology 2024Quote: cDNA of mouse Dnmt3A1WT and DnmtA1W326R (corresponding to human W330R) previously cloned into pCDH-EF1-MCS-Neo (System Biosciences) with an N-terminal FLAG-HA epitope tag(37 ...
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bioRxiv - Bioengineering 2023Quote: ... and the pPACKH1 HIV Lentivector Packaging Kit (System Biosciences) was used to produce lentivirus particles ...
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bioRxiv - Cancer Biology 2020Quote: ... Double-stranded oligonucleotides were cloned into the linearized SmartNuclease vector (EF1-T7-hspCas9-T2A-RFP-H1-gRNA with red fluorescent protein (RFP, System Biosciences) and IFNLR1-guide RNA (IFNLR1-gRNA ...
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bioRxiv - Cell Biology 2022Quote: For stable expression of mScarlet (mSc) tagged IFT-A proteins (IFT144, IFT140, IFT122, IFT121, and IFT139) and associated mutants using the Super PiggyBac system (System Biosciences), the relevant open reading frames were inserted into the PB-EF1α-MCS-IRES-Neo vector with C-terminal TEV ...
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bioRxiv - Cancer Biology 2023Quote: ... along with two housekeeping genes (Supplementary Table 1; CAS9-PR-1 from System Biosciences). The Kcnn4 and Cas9 mRNA levels for each sample were measured in technical duplicates for each primer set ...
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bioRxiv - Developmental Biology 2020Quote: Human SC102A-1 (System Bioscience) and chimpanzee Sandra A iPSC lines (Camp et al. ...
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bioRxiv - Microbiology 2022Quote: HEK293T (System Biosciences, LV900A-1) and HEK293-Spike cells (SARS-CoV-2 Spike (D614)-expressing 293 cells [293-SARS2-S cells ...
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bioRxiv - Developmental Biology 2020Quote: ... DLD-1 cells were co-transfected with a PiggyBac transposase vector (System Biosciences, PB210PA-1) and a PiggyBac-ABA-CRISPRi vector (25 ...
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bioRxiv - Cell Biology 2023Quote: ... 1 million cells were co-transfected with 1 µg ePiggyBac transposase expression plasmid (System Biosciences) and 1 µg each epB-MCP-mScarletI ...