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Citations for System Biosciences :
101 - 150 of 245 citations for LIR 1 LILRB1 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... and pLL-EF1a-rFLuc-T2A-GFP-mPGK-Puro (LL410PA-1, System Biosciences). Preparation and transduction was completed as previously described,(25 ...
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bioRxiv - Biochemistry 2021Quote: ... and a lentiviral transfer vector CD512-EF1a-RFP (System Biosciences CD512B-1) using Lipofectamine 2000 ...
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bioRxiv - Immunology 2020Quote: ... These constructs were cloned into the episomal vector EEV600A-1 (System Biosciences) into which a puromycin resistance cassette was added so that cell pools expressing the proteins could be rapidly selected ...
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bioRxiv - Cell Biology 2021Quote: ... The vector was modified from the PiggyBac vector PBQM812A-1 (System Biosciences) in which the IRES and downstream GFP were removed (John Shin) ...
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bioRxiv - Microbiology 2022Quote: ... and then further concentrated with polyethylene glycol (System Biosciences, Catalog# LV825A-1) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... and 5 ng μL-1 Cas9 mRNA (System Biosciences, Palo Alto, CA) in 10 mM Tris-HCl pH 8.0 and 0.1 mM EDTA were used for injection into the pronucleus of fertilized mouse zygotes ...
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bioRxiv - Genomics 2022Quote: ... and the Super piggyBac Transposase expression vector (SBI System Biosciences, PB200PA-1,) using the Lipofectamine™ LTX Reagent with PLUS™ Reagent (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... using the AAVS1 Safe Harbor Targeting System (GE622A-1-SBI, System Biosciences). In the first round of genome editing ...
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bioRxiv - Cell Biology 2023Quote: ... PiggyBac vector was purchased from System Biosciences (PB210PA-1; Palo Alto, CA). For transfection ...
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bioRxiv - Biophysics 2020Quote: ... Piggybac plasmid PB513Bm2 was made by removing copGFP from PB513B-1 (System Biosciences) by PCR-based mutagenesis ...
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bioRxiv - Cell Biology 2019Quote: ... was transferred into pCDH-CMV-MCS-EF1-Puro (System Biosciences, cat #CD510B-1) using NheI/NotI sites for virus production.
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bioRxiv - Cell Biology 2021Quote: ... filtered medium was incubated with 5X PEG-it solution (System Biosciences, LV825A-1) overnight and centrifugated at 1500 xg for 30 mins at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: Super PiggyBac™ Transposase Expression Vector was purchased from System Biosciences (PB210PA-1) and added at a ratio of 5 ng/μL of transfection mix ...
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bioRxiv - Cancer Biology 2022Quote: We obtained pCDH-CMV-MCS-EF1α-Puro lentivector from System Biosciences (#CD510B-1). To generate the pCDH-NT5C2 expression construct ...
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bioRxiv - Immunology 2021Quote: ... and concentrated using 5x PEG-it viral precipitation solution (System Biosciences; LV825A-1). Transduction of B cells was performed 12-24 h after stimulation via spinfection at 800 g for 1 h.
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bioRxiv - Cancer Biology 2020Quote: ... Viral particles were precipitated from media using PEG-it (System Biosciences, LV810A-1). CP-A-empty and CP-A-ERBB2 stable cells were generated by transfecting 1×106 CP-A cells with lentiviral particles containing either pHAGE-ERBB2 or pHAGE-empty using polybrene (EMD Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... was transferred into pCDH-CMV-MCS-EF1-Puro (System Biosciences, cat #CD510B-1) using NheI/NotI sites for virus production.
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bioRxiv - Molecular Biology 2020Quote: ... The WPRE 3’-UTR sequence was amplified from the CD813A-1 (System Biosciences) vector.
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bioRxiv - Cell Biology 2023Quote: ... 0.25ml of cold PEG-it Virus Precipitation Solution (LV810A-1-SBI, System Biosciences) was added per 1ml of lentiviral-containing supernatant and kept cool at 4°C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... we used the PCDF1-MCS2-EF1-Puro expression vector (System Biosciences, CD110B-1). We performed a restriction digest and gel extraction as above to the plasmid ...
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bioRxiv - Molecular Biology 2021Quote: ... This plasmid was co-transfected with piggyBac Transposase expression vector (System Biosciences, PB210PA-1) into the PABPC1-AID cell line (sC152-C16) ...
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bioRxiv - Bioengineering 2020Quote: ... viral supernatant was concentrated using PEG-it virus precipitation solution (System Biosciences LV825A-1) according to manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... Ceg3 was inserted into pCDH-CMV-MCS-EF1a-RFP (System Biosciences cat# CD512B-1), which was transfected together with pMD2.G (Addgene plasmid #12259 ...
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bioRxiv - Cell Biology 2020Quote: The transfection of FAPs with pCT-CD63-GFP plasmid (#CYTO120-PA-1, System Biosciences) and with a GFP-control vector (mock ...
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bioRxiv - Molecular Biology 2022Quote: ... EVs were isolated using ExoQuick-TC (EXOTC50A-1, System Biosciences, Palo Alto, CA, USA) according to manufacturer instructions ...
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bioRxiv - Genetics 2019Quote: ... We transfected the plasmids with Super PiggyBac Transposase Expression Vector (System Biosciences, PB210PA-1) using Lipofectamine™ 3000 Transfection Reagent ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and ligated into the CAGs-MCS-Enhanced Episomal Vector (EEV) (System Biosciences, EEV600A-1) linearized with AgeI and KpnI ...
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bioRxiv - Neuroscience 2022Quote: ... and microglial enrichment was conducted per manufacturer’s instructions (System Biosciences, Inc.; Catalog # CSFLOWBASICA-1). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... and HEK 293TN (LV900A-1) cells were purchased from System Biosciences (Palo Alto, USA). Mouse mesothelial cells (MCs ...
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bioRxiv - Genetics 2023Quote: ... cells were transfected with 0.72 μg of piggyBac transposase plasmid (System Biosciences PB210PA-1) and 1.78 μg of pCX4.1 plasmid using Lipofectamine 3000 transfection reagent ...
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bioRxiv - Immunology 2023Quote: ... pCDHv6_PTPRZ1 (derived from pCDH-MSCV-MCS-EF1-GFP-T2A-Puro, System Biosciences, CD713B-1), with packaging plasmids pMDLg/pRRE (RRID:Addgene_12251) ...
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bioRxiv - Bioengineering 2021Quote: ... RFP-expressing GBM12 (GBM12-RFP) was generated by transduction with lentivirus (System Biosciences, LL110VA-1) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... Fusion was performed with the Cold Fusion Cloning Kit (SBI System Biosciences, Cat. #MC010B-1).
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bioRxiv - Cell Biology 2021Quote: Inducible Cas9 iPSCs were generated using an AAVS1 TALE-Nuclease KIT (GE60xA-1; System Biosciences) and an iCas9 HR donor plasmid containing a TRE3G promoter-driven spCas9 with a C-terminal MYC NLS followed by a CMV promoter-driven Tet-On-3G and a neomycin resistance adapted from Ihry et al ...
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bioRxiv - Cell Biology 2023Quote: ... from 3T3-L1 genomic DNA and cloned into the HR110-PA-1 vector (System Biosciences). Primer sequences to amplify the 5′- and 3′-side genomic fragments for homologous recombination are as follows ...
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bioRxiv - Cancer Biology 2023Quote: 2.5×106 IFU/ml of pooled lenti-miRNA lentivirus library (System Biosciences Cat. #PMIRHPLVHT-1) was mixed with 4 mg/mL polybrene (Sigma ...
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bioRxiv - Physiology 2023Quote: ... and 5 µL of de-fibrination reagent (Cat #TMEXO-1, System Biosciences, Palo Alto, CA) was added to the plasma to remove clotting factors prior to the isolation of EVs ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified viral particles were transduced into MIN6 cells using TransDux MAX (LV860A-1; System Biosciences) and cultured for 48-72 hours in complete DMEM (11965-092 ...
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bioRxiv - Immunology 2024Quote: ... The PCR fragments were cloned into a PiggyBac mammalian expression vector (System Biosciences, PB210PA-1), modified to harbor a Secrecon-AA signal peptide111 for secretion at the N-terminus ...
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bioRxiv - Systems Biology 2020Quote: ... GFP gene was amplified from pGreenPuro shRNA Cloning and Expression Lentivector (CMV; System biosciences, SI505A-1) and sub-cloned into ITR-CAG-DEST-IRES-Neomycin-ITR plasmid (generous gift from Marc Supprian Schmidt) ...
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bioRxiv - Neuroscience 2021Quote: ... The viral particles were precipitated from the media using AAVanced concentration reagent (System Biosciences #AAV100A-1) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... in DMEM medium supplemented with 10% FBS depleted of exosomes (System Biosciences, EXO-FBS-250A-1). Cells were maintained in this medium for 24 h before supernatant collection for EV purification ...
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bioRxiv - Immunology 2021Quote: ... A PEG-it Virus Precipitation Solution (Cat# LV810A-1, System Biosciences, Palo Alto, CA 94303, USA) was added to 40ml of virus culture at a 1:4 ratio ...
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bioRxiv - Cancer Biology 2022Quote: ... Media containing virus particles was mixed with PEG-it virus precipitation solution (System Biosciences, #LV810A-1) and incubated at 4°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... These constructs are based on an empty parent minicircle cloning vector (MN100A-1; System Biosciences (SBI), Palo Alto ...
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bioRxiv - Neuroscience 2022Quote: ... supernatants were incubated with 120 μL ExoQuick Exosome Precipitation solution (System Biosciences, Inc.; Catalog # EXOQ5TM-1) for 30 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... supernatant was diluted (4 to 1 ratio v/v) with PEG-it solution (SBI, System Biosciences), vortexed and incubated continuously rotating overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... The CRISPR gRNA (5pg per embryo) and Cas9 mRNA (150pg per embryo, System Biosciences CAS500A-1) were injected into single-cell embryos ...
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bioRxiv - Molecular Biology 2023Quote: ... the SpyCas9-ABE-tracrRNA PiggyBac transposon plasmid and PiggyBac transposase expression plasmid (System Biosciences PB210PA-1) were mixed in a 2:1 molar ratio and transfected into the HEK293T-dGFP reporter cell line using Lipofectamine 3000 (ThermoFisher L3000015) ...
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bioRxiv - Cancer Biology 2023Quote: EVs from biological fluids were isolated using ExoQuick exosome precipitation solution (System Biosciences, SBI-EXOQ5A-1). Plasma and serum samples were centrifuged at 3,000 × g for 15 min to remove cells and cell debris ...