Labshake search
Citations for System Biosciences :
101 - 115 of 115 citations for Human PLA2G4F shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: To generate ESC lines carrying Dox-inducible dCas9, plasmid pT7077 (Hazelbaker et al., 2020) (addgene 137879) was co-transfected with Super piggyBac Transposase expression vector (System Biosciences) using Lipofectamine™ 3000 Transfection Reagent (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... PAAKRVKLD) from pCDH-NLSSV40-sfCherry-NLSMyc-Blast (30) were ligated to pCDH-CMV-MCS-EF1-Hygro (plasmid #CD515B-1, System Biosciences) using the Ligation-Convenience Kit (Nippon Gene ...
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bioRxiv - Cancer Biology 2021Quote: ... was constructed by inserting human HAUSP-WT or HAUSP-CS ORF cDNA into the pCDH-CMV-MCS-EF1a-Puro vector (System Biosciences, CD510B-1). The lentiviral plasmid expressing SOX2 was constructed by cloning human SOX2 ORF cDNA into the pCDH-CMV-MCS-EF1a-Puro vector (System Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... a lentiviral Lenti/anti-miR-31 (antimiR-31) was created using commercial plasmids pLenti/anti-miR-31 (System Biosciences, Palo Alto, CA). A Lenti/anti-miR-Cont (antimiR-Cont ...
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bioRxiv - Neuroscience 2022Quote: A Cas9 expressing HEK293T cell line was generated in a well of a 24-well dish by transfecting 300 ng of pB-CAGGS-Cas9-SV40-BPSV40 vector a long with 100 ng of a plasmid expressing the Piggybac transposase (System Biosciences, LLC) with Lipofectamine 2000 according to manufacturer protocols (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... IL) lentiviruses were grown in human embryonic kidney 293T cells and concentrated using the PEG-it Virus Precipitation Solution (System Biosciences, Mountain View, CA), as previously described (51) ...
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bioRxiv - Cancer Biology 2021Quote: ... the medium was replaced with DMEM without FBS for 1 hour before the plasmids pCDH-GAL-9-T2A-Puro (System Biosciences, CD527A-1) or pLKO ...
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bioRxiv - Synthetic Biology 2020Quote: ... Each of the individual rTetR-effector plasmids was randomly integrated into this reporter line by co-transfecting 250 ng Super PiggyBac Transposase expression vector (System Biosciences #PB200PA-1) and 750 ng of rTetR-effector donor vector ...
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bioRxiv - Synthetic Biology 2020Quote: The plasmids containing the rTetR-effector fusions were cloned into the PB-CMV-MCS-EF1α-Puro PiggyBac vector backbone (System Biosciences #PB510B-1), which was further modified via Gibson Assembly with the following components ...
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bioRxiv - Molecular Biology 2023Quote: ... in which A375 Cas9 cells were co-transfected with the rescue plasmids as well as the Super PiggyBac Transposase Expression Vector (System Bioscience Inc, PB200PA) at a 4:1 ratio utilising Lipofectamine™ LTX reagents (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... 6 X 105 cells were co-transfected with 5 µg of gRNA-containing piggyBac vector and 1 µg of piggyBac transposase plasmid (System Biosciences PB210PA-1) using Lipofectamine 3000 Transfection Reagent ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated overnight at 4°C with 1:1000 primary antibody (CD63 and CD81, rabbit anti-human, EXOAB-CD63A-1/ EXOAB-CD81A-1, System Biosciences, Palo Alto, CA, USA) in TBST ...
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bioRxiv - Cancer Biology 2022Quote: ... a plasmid containing the truncated ATR cDNA (containing the first 779 codons only) and a Kanamycin resistance marker was obtained (System Biosciences, SBI, Palo Alto, CA). This plasmid was built into SBI’s FC500A-1 vector that contains attB sites which allow the target sequence to be stably integrated into mammalian genomes in low copy number when co-transfected with ϕC31 integrase ...
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bioRxiv - Microbiology 2020Quote: ... HEK293T cells (ATCC® ACS-4500™) were transiently transfected with hACE2 fusion BFP encoding PB510 plasmid using PiggyBac Transposon System (System Biosciences, PB210PA-1), followed by addition of 2 μg/mL puromycin 6 h post-transfection ...
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bioRxiv - Cancer Biology 2023Quote: The viral vector encoding the anti-CD117 CAR and the RQR8 sequences previously cloned in the pCDH-EF1α-MCS-T2A-GFP lentiviral plasmid (System Biosciences, Palo Alto, CA, USA) as produced as previously reported 14 ...