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Citations for System Biosciences :
51 - 100 of 284 citations for Human Immunodeficiency Virus Nef Protein HIV 1 Clade B IIIB since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... Lentiviral libraries were prepared as described above in complete DMEM and harvested using PEG-it Virus Precipitation Solution (System Biosciences). Lentiviral titers were checked using the hFzd5CRD-1TM cells at 2 ml scale based on Wnt expression ...
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bioRxiv - Neuroscience 2021Quote: ... Lentiviral particles were harvested either by ultracentrifugation at 106,559 g for 2 h at 4°C using a Beckman SW 32 Ti rotor or using the PEG-it Virus Precipitation Solution (System Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Medium containing lentiviral particles was collected at 48 and 72 hours post transfection and concentrated using PEG-it Virus Precipitation Solution (System BioSciences). Precipitated lentiviral particles were resuspended in DMEM ...
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bioRxiv - Immunology 2022Quote: ... VLPs were precipitated from the clarified supernatant by the addition of PEG-it Virus Precipitation Solution (System Biosciences, Palo Alto, CA, USA) at a ratio of 1:10 ...
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bioRxiv - Cancer Biology 2022Quote: The infection of HuCCT1 cells with pGreenFire 2.0 TCF/LEF reporter virus (pGF2-TCF/LEF-rFluc-T2A-GFP-mPGK-Puro) (SBI System Biosciences, TR413VA-P) was performed using SureENTRY Transduction Reagent ...
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bioRxiv - Molecular Biology 2020Quote: MDEs were isolated from G1-G3 bovine and human milk samples (1.5 mL/sample) using the EQ reagent (System Biosciences: EXOQ5A-1) (13,14,38,40,44) ...
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bioRxiv - Molecular Biology 2019Quote: HeLa cervix carcinoma cells (American Tissue Culture Collection: Manassas, VA, USA) and human embryonic kidney (HEK) 293TN cells (System Biosciences, LV900A-1) were cultured using Dulbecco’ s Modified Eagle Medium (DMEM ...
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bioRxiv - Cancer Biology 2021Quote: ... was constructed by inserting human HAUSP-WT or HAUSP-CS ORF cDNA into the pCDH-CMV-MCS-EF1a-Puro vector (System Biosciences, CD510B-1). The lentiviral plasmid expressing SOX2 was constructed by cloning human SOX2 ORF cDNA into the pCDH-CMV-MCS-EF1a-Puro vector (System Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... The lentiviral plasmid expressing SOX2 was constructed by cloning human SOX2 ORF cDNA into the pCDH-CMV-MCS-EF1a-Puro vector (System Biosciences, CD510B-1) or PCW57.1 vector (Addgene ...
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bioRxiv - Cell Biology 2021Quote: ... Virus-containing supernatants were either collected or concentrated by mixing with quarter volumes of 5 × PEG-it Virus Precipitation Solution (System Biosciences, Palo Alto, CA, United States), followed by centrifugation according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... Cas9 protein was bought from System Biosciences (2438 Embarcadero Way ...
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bioRxiv - Immunology 2019Quote: ... and used to transduce pre-activated primary human CD4+ T cells in combination with Transdux (System Biosciences). GFP+ cells were FACS sorted 48h later ...
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bioRxiv - Neuroscience 2022Quote: shRNAs targeting human HNRNPR were cloned into a modified version of pSIH-H1 shRNA vector (System Biosciences) containing EGFP according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: shRNAs targeting human HNRNPR were cloned into a modified version of pSIH-H1 shRNA vector (System Biosciences) containing EGFP according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... human ATRX cDNA were amplified from HeLa and assembled into PB-EF1α-MCS-IRES-Neo vector (System Biosciences) by Gibson assembly ...
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bioRxiv - Cancer Biology 2022Quote: ... Both human and mouse BGN were subcloned into the PB-CMV-MCS-EF1α-Puro piggybac expression vector (System Biosciences) using the 5’ EcoRI and 3’ BamHI restriction sites ...
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bioRxiv - Cancer Biology 2024Quote: cDNA of mouse Dnmt3A1WT and DnmtA1W326R (corresponding to human W330R) previously cloned into pCDH-EF1-MCS-Neo (System Biosciences) with an N-terminal FLAG-HA epitope tag(37 ...
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bioRxiv - Cancer Biology 2022Quote: ... Human wild-type or mutant (K4M) histone H3.1 were cloned into pCDH-EF1-MCS-IRES-RFP (System Biosciences, CD531A-2). To express sgRNAs ...
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bioRxiv - Molecular Biology 2021Quote: The primary human T cell transductions were done with multiple viral titers using TransDux™ MAX Lentivirus Transduction Reagent (System Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: The gene for human HBEGF was synthesized and cloned into the MCS of PiggyBac vector PB-CMV-MCS-EF1α-Puro (System Biosciences) by restriction digest with EcoRI and BamHI (pCMV-hHBEGF) ...
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bioRxiv - Developmental Biology 2022Quote: ... The gRNA (5 pg/embryo) and mRNA encoding Cas9 protein (150 pg/embryo, System Biosciences) were injected into one-cell stage embryos ...
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bioRxiv - Molecular Biology 2021Quote: ... exosomes from ASCs were labeled fluorescent red using an ExoGlow-Protein EV Labeling Kit (System Biosciences) and then incubated with precipitation solution at 4°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... with 1/5 final dilution (System Biosciences, LV810A-1), and the mixture was incubated at 4 °C for 6 hrs ...
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bioRxiv - Molecular Biology 2019Quote: ... with 1 μg of transposase plasmid (System Biosciences #PB210PA-1) under conditions described above ...
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bioRxiv - Cell Biology 2022Quote: ... a lentivirus expression plasmid (MMIR-1a-1-PA-1 and MMIR-206-PA-1 purchased from System Biosciences) and plasmid mixture for packaging (ViraPower HiPerform Lentiviral Expression Systems ...
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bioRxiv - Biochemistry 2020Quote: ... anti-TSG101 antibody (EXOAB-TSG101-1; System Biosciences, CA; 1:500), anti-CD63 antibody (EXOAB-CD63A-1 ...
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bioRxiv - Biochemistry 2020Quote: ... anti-CD63 antibody (EXOAB-CD63A-1; System Biosciences, CA; 1:500), anti-CD5L antibody (AF2834 ...
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bioRxiv - Cell Biology 2021Quote: ... AP100R-1 (System Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Lentivector-based scrambled miRZip-000 (MZIP000-PA-1) and miRZip-146a (anti-miRNA; MZIP146a-PA-1) in S1505A-1 construct vector were purchased from System Biosciences. Both Scrambled and miRZIP vectors contained copGFP as a fluorescent reporter ...
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bioRxiv - Cancer Biology 2020Quote: ... Double-stranded oligonucleotides were cloned into the linearized SmartNuclease vector (EF1-T7-hspCas9-T2A-RFP-H1-gRNA with red fluorescent protein (RFP, System Biosciences) and IFNLR1-guide RNA (IFNLR1-gRNA ...
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bioRxiv - Cell Biology 2022Quote: For stable expression of mScarlet (mSc) tagged IFT-A proteins (IFT144, IFT140, IFT122, IFT121, and IFT139) and associated mutants using the Super PiggyBac system (System Biosciences), the relevant open reading frames were inserted into the PB-EF1α-MCS-IRES-Neo vector with C-terminal TEV ...
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bioRxiv - Cancer Biology 2023Quote: ... along with two housekeeping genes (Supplementary Table 1; CAS9-PR-1 from System Biosciences). The Kcnn4 and Cas9 mRNA levels for each sample were measured in technical duplicates for each primer set ...
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bioRxiv - Microbiology 2022Quote: HEK293T (System Biosciences, LV900A-1) and HEK293-Spike cells (SARS-CoV-2 Spike (D614)-expressing 293 cells [293-SARS2-S cells ...
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bioRxiv - Developmental Biology 2020Quote: ... DLD-1 cells were co-transfected with a PiggyBac transposase vector (System Biosciences, PB210PA-1) and a PiggyBac-ABA-CRISPRi vector (25 ...
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bioRxiv - Cell Biology 2023Quote: ... 1 million cells were co-transfected with 1 µg ePiggyBac transposase expression plasmid (System Biosciences) and 1 µg each epB-MCP-mScarletI ...
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bioRxiv - Developmental Biology 2023Quote: ... or goat anti-rabbit (1:20,000; EXOAB-KIT-1; System Biosciences, Palo Alto, CA, USA). Protein signals were detected using the SuperSignal West Femto Chemiluminescent kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: HEK293TN cells (System Biosciences, LV900A-1) and HeLA cells (American Tissue Culture Collection ...
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bioRxiv - Molecular Biology 2019Quote: HEK293TN cells (System Biosciences, LV900A-1) were cultured using Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Synthetic Biology 2020Quote: ... Exo-Check Array: A549 protein markers were characterised using a commercially sourced Exo-Check Exosome Antibody Array kit (#EXORAY200A-4, System Biosciences, CA, USA). A549 EVs (100 μg ...
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bioRxiv - Neuroscience 2020Quote: ... fibrinogen and fibrin proteins known to impede efficient EV recovery were removed using 5 U/mL of thrombin (System Biosciences, Inc.; Mountainview, CA) for 30 minutes at RT followed by addition of 495 μL of Dulbecco’s PBS-1X (DPBS ...
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bioRxiv - Genomics 2022Quote: THP-1 cells were transduced in triplicates using the TransDux MAX transfection reagent (System Biosciences, LV860A-1) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2019Quote: The identification of protein markers on the isolated exosomes was undertaken using the commercially available Exo-Check Exosome Ab Array kit (System Biosciences, Palo Alto, CA), as described by the manufacturer ...
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bioRxiv - Genetics 2021Quote: ... along with 1/10 total plasmid mass of Super PiggyBac Transposase Expression Vector (System Bioscience, Inc. #PB210PA-1). Stable integrants were selected with 1-2 μg/mL puromycin.
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bioRxiv - Cell Biology 2022Quote: ... Stock cumate solution (System Biosciences # QM100A-1) was diluted to 5X in culture medium prior to use.
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bioRxiv - Cell Biology 2021Quote: ... with piggyBac transposase (PB210PA-1, System Biosciences) and a piggyBac plasmid expressing SpCas9 under a doxycycline inducible promoter and containing a blasticidin selection cassette ...
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bioRxiv - Immunology 2021Quote: IL-23 EEV(EEV651A-1, System Biosciences) was hydrodynamically injected in a volume of sterile HBSS (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... and HEK293TN cells (System Biosciences, #LV900A-1) were routinely cultured in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Molecular Biology 2023Quote: ... ni piggyBac transposase (PB200PA-1; System Biosciences) (2) ...
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bioRxiv - Microbiology 2023Quote: ... and HR110PA-1 (System Biosciences, CA, USA). The pX330-puro vector was constructed by integrating the puromycin resistance gene sequence from the HR110PA-1 vector into the pX330 vector ...
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bioRxiv - Cancer Biology 2024Quote: ... and 0.5% TransDux (LV860A-1, System Biosciences). 72 hours after transduction ...