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Citations for System Biosciences :
201 - 250 of 285 citations for Human Eukaryotic Translation Initiation Factor 1 EIF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... cells were transfected with 0.2 μg of Super PiggyBac Transposase Expression Vector (System Biosciences, PB210PA-1) and 0.2 ...
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bioRxiv - Molecular Biology 2019Quote: The identification of protein markers on the isolated exosomes was undertaken using the commercially available Exo-Check Exosome Ab Array kit (System Biosciences, Palo Alto, CA), as described by the manufacturer ...
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bioRxiv - Cancer Biology 2021Quote: ... The transfer vector was then packaged into a lentivirus using the pPACKH1 HIV third generation lentiviral expression system kit and PureFection reagent (both from System Biosciences, Palo Alto, CA) into HEK293-T cells (ATCC ...
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bioRxiv - Cancer Biology 2023Quote: EV pellets were diluted and 45 µL was examined with the ExoELISA-ULTRA CD63 Kit according to the manufacturer’s instructions (System Biosciences, Palo Alto, CA, USA). The sample absorbance minus the blank wells was used to determine concentrations based on the standard curve included in the kit.
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... We created the PB transposase plasmid pSPBase by removing the ccdB gene from PB210PA-1 (System Biosciences) to render it generally propagable ...
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bioRxiv - Genetics 2021Quote: ... An in vitro transcribed sgRNA targeting Slc16a11 (5’-AGTCCTAACCTCGCTTGGCT-3’) and hspCas9 mRNA (CAS500A-1, System Biosciences) were microinjected into C57BL/6J mouse zygotes by the Genome Modification Facility at Harvard University ...
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bioRxiv - Cell Biology 2021Quote: ... MNT1 cells were transfected with Pb-TRE3G-SpCas9-IRES-Blast and piggyBac transposase (PB210PA-1, System Biosciences) in 1:3 ratio using lipofectamine (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... cDNA sequences were cloned into the PB-CMV-MCS-EF1α-Puro vector (PB510B-1, SBI System Biosciences) where the CMV promoter was replaced by a PGK promoter ...
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bioRxiv - Genomics 2021Quote: ... was stably introduced in HEK293T cells using the piggy-transposase system (System Biosciences, cat. no. PB210PA-1) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... the gfp-lem8 fusion was inserted into pCDH-CMV-MCS-EF1a-Puro (System Biosciences, cat# CD510B-1). The plasmids were co-transfected with pMD2.G (gift from Dr ...
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bioRxiv - Genomics 2020Quote: ... multiple viral supernatants were collected and concentrated using PEG-it Virus Precipitation Solution (System Biosciences LV810A-1). Cells were infected 2-3 times at 12-hour intervals before selection in the appropriate antibiotic.
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bioRxiv - Cell Biology 2020Quote: ... collected after 24 hours and concentrated using Peg-It Virus precipitation solution (cat# LV810A-1, System Biosciences) according to the manufacturer’s manual.
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bioRxiv - Immunology 2024Quote: ... The HEK293TN-hACE2 cell line was generated by lentiviral transduction of HEK293TN (System Bioscience, Cat#LV900A-1) cells as described in Notarbartolo S ...
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bioRxiv - Cancer Biology 2020Quote: Exosomes were purified from cultured medium supplemented with exosome-depleted FBS using the exosome purification kit following manufacturer’s instructions (ExoQuick™, System Bioscience, Mountain View, CA, USA).
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bioRxiv - Molecular Biology 2020Quote: ... we replaced the KRAB and dCas9 cassettes from the pHR-SFFV-KRAB-dCas9-mCherry vector with the HA-tag-MEOX1 cassette using Cold-fusion cloning kit (SBI System BioSciences, Palo Alto, CA, USA) following the instruction provided by the manufacturer ...
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bioRxiv - Developmental Biology 2022Quote: ... with 250 μg protein equivalent of AFSC-EVs stained using 2 μL of ExoGlow™-Vivo (Near IR) EV Labeling Kit (System Biosciences, Palo Alto, CA).
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bioRxiv - Cell Biology 2021Quote: ... Cas9-MNT1 cells were transfected with Pb459-U6-sgRNA-EF1a-PuroR and piggyBac transposase (PB210PA-1, System Biosciences) in a 1:3 ratio using lipofectamine (Life Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... Culture medium was collected 72 hours post transfection and concentrated with AAVanced Concentration Reagent (#AAV100A-1, System Biosciences).
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bioRxiv - Cell Biology 2020Quote: Lentivirus was generated with PEG-it Virus Precipitation Solution (System Biosciences, Palo Alto, CA, USA no LV810A-1) using a LV H2B-GFP plasmid (Addgene no 25999 ...
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bioRxiv - Immunology 2021Quote: Spike-expressing pseudovirus was generated with System Bioscience’s pPACK-SPIKE packaging system (System Biosciences, Cat # CVD19-50A-1) as per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... BAE-FITC complexes were incubated with 350 μL of Exosome elution buffer (System Biosciences, Inc.; Catalog # CSFLOWBASICA-1) at 25°C for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviruses containing the luciferase expression cassette were produced in 293 TN cells (System Bioscience, catalog number: LV900A-1) by transient transfection with the PSMPUW-Hygro lentiviral vector carrying the luciferase gene and the ViraSafe™ Lentiviral Packaging System (Cell Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... Viral transduction of matured iPS-RPE were aided using TransDux virus transduction reagent (System Biosciences, cat# LV850A-1) and spin infection (800 g ...
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bioRxiv - Cell Biology 2022Quote: ... The pCDH-CMV-MCS-EF1-Blast was generated from pCDH-CMV-MCS-EF1-Hygro (plasmid #CD515B-1; System Biosciences). The annealed synthetic oligonucleotides (Table 2 ...
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bioRxiv - Molecular Biology 2021Quote: ... The WPRE (Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element) 3’-UTR sequence was amplified from the CD813A-1 (System Biosciences) vector.
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... we developed the excision-only PB transposase plasmid pXPBase-GFP through multiple cloning steps modifying PB220PA-1 (System Biosciences). The ccdB gene was removed to render the plasmid generally propagable ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNAs were then cloned into the peGFP-N1 backbone by Cold Fusion Cloning (System Biosciences, Cat# MC010B-1) following the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2022Quote: ... supernatant containing eluted exosomes were incubated with 1 μL of Exo-FlowIP clearing reagent (System Biosciences, Inc.; Catalog # EXOFLOW32A) at 37°C for 30 min then stored at −80°C.
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bioRxiv - Biochemistry 2023Quote: ... Stable cell lines were generated by cotransfection with the Super PiggyBac Transposase Expression Vector (cat# PB210PA-1, System Biosciences) followed by selection via addition of 5 μg/mL puromycin (cat# ant-pr-1 ...
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bioRxiv - Developmental Biology 2023Quote: ... 2000 ng of the PiggyBac vector plasmid and 500 ng of the PiggyBac transposase plasmid (System Biosciences, PB210PA-1) were mixed in 125 μL Opti-MEM (Gibco ...
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bioRxiv - Genomics 2023Quote: ... The collected virus was concentrated 100-fold after precipitation with PEG-itTM Virus Precipitation Solution (System BioSciences LV810A-1) for 3 days ...
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bioRxiv - Bioengineering 2023Quote: Stable HeLa-Fucci cell line was generated using the Super PiggyBac Transposase Expression Vector system (System Biosciences, PB210PA-1) together with tFucci(CA)5 plasmid29 (Addgene #153521) ...
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bioRxiv - Biophysics 2023Quote: ... Cells were transfected with 1500ng of HaloTag-NLS or HaloTag-H2b + 1000ng of piggyBac Transposase (System Biosciences, PB210PA-1) using Lipofectamine2000 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: Cells were cultured in DMEM/1640 supplemented with 10% exosome-depleted FBS (EXO-FBS-250A-1, SBI System Biosciences, USA) using 10×10 cm2 disks (1×106 cells per disk ...
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bioRxiv - Biochemistry 2020Quote: ... and also cloned into pCDH-MSCV-MCS-EF1α-GFP+Puro lentiviral vector (System Biosciences, Cat# CD713B-1, Palo Alto, CA) for constitutive expression ...
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bioRxiv - Genetics 2019Quote: To produce lentiviral cell lines containing pGreenFire1™ Pathway Reporter lentivector (Cat#TR010PA-N and Cat#TR000PA-1, System Bioscience) expressing the MUC5B promoter region and controls ...
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bioRxiv - Neuroscience 2022Quote: ... The lentivirus was concentrated with the use of the PEG-it virus precipitation solution (2.5 ml, System Biosciences, LV810A-1) for 2-3 days at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The N-term Flag-HA-MED4 cDNA was sub-cloned in pCDH-EF1-MCS-IRES-Neo (System Biosciences; CD502A-1) and verified by sequencing ...
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bioRxiv - Genetics 2023Quote: The PB EGFP donor vector (PB-CMV-MCS-EF1ɑ-GFP, #PB511B-1) was purchased from System Biosciences (Palo Alto, CA). The PiggyBac transposase (PBase ...
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bioRxiv - Immunology 2021Quote: ... 5’ ATACGCGGATCCtcagtagggccgccacac 3’ and cloned into a commercial lentivector containing a GFP reporter gene (pCDH-GFP-Em-CD513B-1 from System Biosciences). To overexpress RUNX2 in human T cells ...
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bioRxiv - Genomics 2020Quote: ... overnight at 37°C and cloned into a linearized pCDH-EF1alpha-MCS-BGH-PGK-GFP-T2A-Puro lentivector (CD550A-1, System Biosciences) using T4 DNA ligase (New England BioLabs ...
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bioRxiv - Cancer Biology 2020Quote: The piggyBac transposon vector was constructed by inserting a random 10-bp DNA barcode sequence between the CMV promoter and the multiple cloning site of the piggyBac dual promoter vector (PB513B-1; System Biosciences). The GFP sequence in the piggyBac vector was deleted by a site-directed mutagenesis technique for the direct repeat–green fluorescent protein (DR-GFP ...
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bioRxiv - Biophysics 2021Quote: ... so that high expression of the binder could be achieved only after addition of the cumate inducer (QM100A-1, System Biosciences). (ArrayG/N)16x has a total size of 255.6 kDa and of 689.2 kDa when fully occupied with mwGFP ...
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bioRxiv - Cell Biology 2021Quote: TYR-G1-G4 knockout cell lines were transfected with the Pb-TRE3G-TYR-HygR plasmid and piggyBac transposase (PB210PA-1, System Biosciences) in a 1:3 ratio using lipofectamine (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... the piggyBac transposon plasmid that contains the desired transgene and a piggyBac transposase plasmid (PB210PA-1, System Biosciences, Palo Alto, CA) were electroporated into TP53-/- RPE-1 cells simultaneously using the Neon electroporation system according to the manufacturer’s instruction (Invitrogen) ...
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bioRxiv - Bioengineering 2022Quote: Gene editing of COS-7 cells were performed by electroporation of COS-7 cells with Super PiggyBac plasmid (PB210PA-1, System Biosciences) and one of the 5 plasmids of pBv1-EF-6X ...
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bioRxiv - Neuroscience 2022Quote: ... HEK 293T cells cultured in 10-cm Petri dishes at a 50-60% confluence were co-transfected with 10 μg lentiviral plasmid expressing miR-124-1 precursor under the CMV promoter and GFP under the EF1 promoter (SBI System Biosciences) or 10 μg lentiviral plasmid expressing GFP under the CMV promoter and the packaging plasmids pVSV-G (3.5 μg) ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was blocked in freshly prepared 5% BSA diluted in TBS before being immunoblotted with specific primary antibodies (CD81; #EXOAB-CD81A-1 System Biosciences, GM130 ...
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bioRxiv - Neuroscience 2022Quote: ... The open reading frame from of murine XPR1 was tagged with a sequence for the HA-epitope and cloned into PiggyBac expression vector PB510B-1 (System Biosciences) with XbaI and NotI ...
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bioRxiv - Developmental Biology 2020Quote: ... The resulting PB510B-based sgRNA expression vector and PB533-dCas9-EGFP were co-transfected with a transposase expression vector PB200PA-1 (System BioSciences) into MC12 cells ...