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Citations for System Biosciences :
251 - 300 of 319 citations for Dengue Virus Serotype 1 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: The 3’UTR reporter plasmids were co-transfected into HEK293T cells using PEI max together with the indicated pCDH-EF1-MCS expressing plasmids (System Biosciences) containing a cloned pre-miRNA gene of interest ...
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bioRxiv - Immunology 2022Quote: ... encoding HLA-A*02:01 or HLA-A*03:01 by spinoculation with ultracentrifuge concentrated viral particles produced from 293T-HEK-TN cells (System biosciences), followed by sorting of cells stained with anti-human HLA-A ...
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bioRxiv - Developmental Biology 2019Quote: We acquired 6 human induced pluripotent stem cell (iPSC) lines (Sojd3, Hoik1, Kucg2, Wibj2 from the HipSci resource54; h409b2 from the RIKEN BRC cell bank17; Sc102a1 from System Biosciences), one human ES cell line (H9 ...
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bioRxiv - Cell Biology 2022Quote: ... one day before the transfection of the expression construct of mCherry-proliferating-cell nuclear antigen (PCNA) or EGFP-ATM based on PB533A-2 (System Biosciences) with a PiggyBac transposon expression vector (PB210PA-1 ...
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bioRxiv - Bioengineering 2023Quote: ... The in vivo T cell activation reporter was constructed by cloning a sequence containing firefly luciferase into the pGreenFire1-NF-κB lentiviral vector (System Biosciences) under the NF-κB responsive promoter ...
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bioRxiv - Bioengineering 2023Quote: ... were purchased from the Riken Brain Science Institute (Japan) and used to generate lentivirus particles by co-transfecting HEK 293TN cells (System Biosciences) with packaging (psPAX2 ...
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bioRxiv - Cell Biology 2024Quote: Stable cell lines expressing GFP-tagged cytoplasmic tail chimeras under a cumate-inducible promoter were generated by PiggyBac transposition (System Biosciences). Wild-type ...
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bioRxiv - Cell Biology 2022Quote: ... The pCDH-CMV-MCS-EF1-Blast was generated from pCDH-CMV-MCS-EF1-Hygro (plasmid #CD515B-1; System Biosciences). The annealed synthetic oligonucleotides (Table 2 ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... we developed the excision-only PB transposase plasmid pXPBase-GFP through multiple cloning steps modifying PB220PA-1 (System Biosciences). The ccdB gene was removed to render the plasmid generally propagable ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNAs were then cloned into the peGFP-N1 backbone by Cold Fusion Cloning (System Biosciences, Cat# MC010B-1) following the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2022Quote: ... supernatant containing eluted exosomes were incubated with 1 μL of Exo-FlowIP clearing reagent (System Biosciences, Inc.; Catalog # EXOFLOW32A) at 37°C for 30 min then stored at −80°C.
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bioRxiv - Developmental Biology 2023Quote: ... 2000 ng of the PiggyBac vector plasmid and 500 ng of the PiggyBac transposase plasmid (System Biosciences, PB210PA-1) were mixed in 125 μL Opti-MEM (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... was added to a tube along with purefection reagent and pPackF1 lentiviral packaging kit plasmids (System Biosciences, # LV100A-1) and either PCF1 (empty vector control) ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... Quantification of successfully integrated piggyBac transposons was performed using a piggyBac qPCR copy number kit (System Biosciences, PBC100A-1) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... Frozen primary human T cell pellets were thawed and then stimulated with ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (Stemcell) for 48 hours and then transduced with various CAR viruses using TransDux™ MAX Lentivirus Transduction Reagent (System Biosciences) as described above ...
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bioRxiv - Biochemistry 2020Quote: ... and also cloned into pCDH-MSCV-MCS-EF1α-GFP+Puro lentiviral vector (System Biosciences, Cat# CD713B-1, Palo Alto, CA) for constitutive expression ...
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bioRxiv - Cancer Biology 2023Quote: ... The N-term Flag-HA-MED4 cDNA was sub-cloned in pCDH-EF1-MCS-IRES-Neo (System Biosciences; CD502A-1) and verified by sequencing ...
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bioRxiv - Genetics 2023Quote: The PB EGFP donor vector (PB-CMV-MCS-EF1ɑ-GFP, #PB511B-1) was purchased from System Biosciences (Palo Alto, CA). The PiggyBac transposase (PBase ...
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bioRxiv - Microbiology 2021Quote: ... EBV-negative Akata(-) cells were treated with purified exosomes in RPMI1640 supplemented with 5% exosome-depleted FBS (System Biosciences, Palo Alto, CA, USA). After 6 h ...
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bioRxiv - Molecular Biology 2023Quote: ... in which A375 Cas9 cells were co-transfected with the rescue plasmids as well as the Super PiggyBac Transposase Expression Vector (System Bioscience Inc, PB200PA) at a 4:1 ratio utilising Lipofectamine™ LTX reagents (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... 5’ ATACGCGGATCCtcagtagggccgccacac 3’ and cloned into a commercial lentivector containing a GFP reporter gene (pCDH-GFP-Em-CD513B-1 from System Biosciences). To overexpress RUNX2 in human T cells ...
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bioRxiv - Molecular Biology 2021Quote: ... EVs were isolated from tissue culture media using a commercial kit containing a polyethylene glycol (PEG) solution (ExoQuick-TC ULTRA kit, EQULTRA-20TC-1; System Biosciences) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2020Quote: ... overnight at 37°C and cloned into a linearized pCDH-EF1alpha-MCS-BGH-PGK-GFP-T2A-Puro lentivector (CD550A-1, System Biosciences) using T4 DNA ligase (New England BioLabs ...
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bioRxiv - Cancer Biology 2020Quote: The piggyBac transposon vector was constructed by inserting a random 10-bp DNA barcode sequence between the CMV promoter and the multiple cloning site of the piggyBac dual promoter vector (PB513B-1; System Biosciences). The GFP sequence in the piggyBac vector was deleted by a site-directed mutagenesis technique for the direct repeat–green fluorescent protein (DR-GFP ...
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bioRxiv - Biophysics 2021Quote: ... so that high expression of the binder could be achieved only after addition of the cumate inducer (QM100A-1, System Biosciences). (ArrayG/N)16x has a total size of 255.6 kDa and of 689.2 kDa when fully occupied with mwGFP ...
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bioRxiv - Cell Biology 2021Quote: ... the piggyBac transposon plasmid that contains the desired transgene and a piggyBac transposase plasmid (PB210PA-1, System Biosciences, Palo Alto, CA) were electroporated into TP53-/- RPE-1 cells simultaneously using the Neon electroporation system according to the manufacturer’s instruction (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... HEK 293T cells cultured in 10-cm Petri dishes at a 50-60% confluence were co-transfected with 10 μg lentiviral plasmid expressing miR-124-1 precursor under the CMV promoter and GFP under the EF1 promoter (SBI System Biosciences) or 10 μg lentiviral plasmid expressing GFP under the CMV promoter and the packaging plasmids pVSV-G (3.5 μg) ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was blocked in freshly prepared 5% BSA diluted in TBS before being immunoblotted with specific primary antibodies (CD81; #EXOAB-CD81A-1 System Biosciences, GM130 ...
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bioRxiv - Neuroscience 2022Quote: ... The open reading frame from of murine XPR1 was tagged with a sequence for the HA-epitope and cloned into PiggyBac expression vector PB510B-1 (System Biosciences) with XbaI and NotI ...
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bioRxiv - Molecular Biology 2022Quote: RNA was isolated from the EVs using the Exoquick RNA column purification kit (EQ808A-1, System Biosciences, Palo Alto, CA, USA). Purified RNA was quantified using a Bioanalyzer and the Agilent RNA 6000 Pico kit (5067-1513 ...
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bioRxiv - Developmental Biology 2020Quote: ... The resulting PB510B-based sgRNA expression vector and PB533-dCas9-EGFP were co-transfected with a transposase expression vector PB200PA-1 (System BioSciences) into MC12 cells ...
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bioRxiv - Microbiology 2021Quote: For cDNA expression of WIPI2B proteins cells were transduced with lentivirus carrying the gene of interest with an N-terminal 3X Ty1 tag (Table S7) on the pCDH-CMV-MCS-T2A-Puro backbone (CD522A-1, System Biosciences). cDNA expression of ATG proteins has been previously described (21 ...
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bioRxiv - Cell Biology 2023Quote: ... The 30 mL of lentiviral supernatant was pooled and concentrated to 1/100th of its original volume (300 µL) in DPBS using PEG-iT (System Biosciences), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: Clones A and E were transfected with a Piggybac-PEmax plasmid with the Piggybac transposase (System Biosciences, Cat. No. PB210PA-1) at a 10:1 molar ratio using the transIT-LT1 transfection reagent (Mirius Bio ...
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bioRxiv - Cancer Biology 2023Quote: ... HA1_BsaI_rev 5’-TAT AGA GAC CGA TGG ATA AAA GCC CAA ATT C-3’ and cloned into the multiple cloning site 1 (MCS1) of the vector MCS1-EF1α-GFP-T2A-Puro-pA-MCS2-PGK-hsvTK (HR700, System Biosciences) using BsrGI (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... U2-OS cells (UC Berkeley BDS Cell Culture Facility) cultured in DMEM (1 g/L glucose, 10% FBS) were transfected with a modified PiggyBac Transposase plasmid (System Biosciences) and a PGK.mNeonGreen-LacI.NLS.IRES.Puro integration plasmid using Fugene6 transfection reagent ...
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bioRxiv - Neuroscience 2024Quote: ... and the medium was changed to the same DMEM but supplemented with 10% exosome-depleted FBS (EXO-FBS-50A-1, System Bioscience). The media was collected on the day of isolation ...
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bioRxiv - Neuroscience 2024Quote: The mouse H3f3a sequence (encoding the histone H3.3A isoform) upstream an IRES and nuclear GFP sequences were cloned into the EcoRI and NotI sites of EEV600A-1 (System Biosciences) to generate the EEV expressing the WT histone H3.3A (supplementary Fig ...
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bioRxiv - Developmental Biology 2024Quote: ... with the primers 5’-GGGGAATTCGCCACCATGGGTTCTCA-3’ and 5’-CCC GCGGCCGCTCACTTCGCTGTCATCA-3’ was subcloned into EcoRI and NotI sites in the P B-CMV-MCS-EF1α-Puro PiggyBac transposon vector (PB510B-1, System Bioscience).
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bioRxiv - Cell Biology 2023Quote: ... designed by CRISPRdirect,101 were cloned into linearized CAG-T7-hspCas9-T2A-RFP-H1-gRNA SmartNuclease vector (CAS721R-1; System Biosciences).
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bioRxiv - Cell Biology 2024Quote: ... PAAKRVKLD) from pCDH-NLSSV40-sfCherry-NLSMyc-Blast (30) were ligated to pCDH-CMV-MCS-EF1-Hygro (plasmid #CD515B-1, System Biosciences) using the Ligation-Convenience Kit (Nippon Gene ...
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bioRxiv - Cancer Biology 2020Quote: ... MDA-MB-231 and MDA-MB-231-Luc+ cells were transduced with lentivirus vector expressing a GFP or RFP tag fused to the membrane-associated tetraspanin CD63 (System Biosciences, Palo Alto, CA). Breast cancer cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Developmental Biology 2021Quote: ... The amplicon was cloned into a modified version of the self-inactivating lentiviral vector pPS-EF1α-GFP-RFP (System Biosciences, LV603PA-1) where GFP and RFP had been removed and replaced by an hPGK-Puromycin cassette (pSIN-EF1a promoter-BGH polyA-hPGK-Puromycin) ...
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bioRxiv - Molecular Biology 2020Quote: MDEs were isolated from G1-G3 bovine and human milk samples (1.5 mL/sample) using the EQ reagent (System Biosciences: EXOQ5A-1) (13,14,38,40,44) ...
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bioRxiv - Cell Biology 2023Quote: ... The tag donor was also introduced as a minicircle generated with the MC-Easy Minicircle DNA Production Kit according to manufacturer’s instructions (System Biosciences, MN920A-1). Both solutions were combined ...
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bioRxiv - Cell Biology 2023Quote: ... The tag donor was also introduced as a minicircle generated with the MC-Easy Minicircle DNA Production Kit according to manufacturer’s instructions (System Biosciences, MN920A-1). Both solutions were combined ...
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bioRxiv - Immunology 2023Quote: ... Inserts were introduced into the pCDH-CuO-MCS-EF1a-CymR-T2A-Puro SparQ™ All-in-one Cloning and Expression Lentivector (QM800A-1, System Biosciences). The correct sequence of the gene of interest (GOI ...
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bioRxiv - Cell Biology 2021Quote: ... Not1 digested and Calf Intestinal Phosphatase treated pCDH-CMV-MCS-EF1α-copGFP Dual Promoter Cloning and Expression Lentivector (System Biosciences LLC, #CD511B-1). Similarly ...
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bioRxiv - Biophysics 2022Quote: ... wildtype U-2 OS cells (ATCC #HTB-96) were transfected with 1000 μg of PiggyBac-HaloTag GR and 250 μg of Super PiggyBac Transposase (System BioSciences #PB200A-1) and 3 μL of TransIt LT1 (Mirus #MIR2305) ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ACE2 genes were cloned into pCDH-EF1α-MCS-BGH-PGK-GFP-T2A-Puro Cloning and Expression Lentivector (System Biosciences, catalogue number CD550A-1), where the EF1α promoter was replaced with the full length EF1α using NheI and ClaI restriction enzymes (New England Biolabs) ...