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Citations for System Biosciences :
101 - 150 of 288 citations for Dengue Virus Serotype 1 Envelope Protein Human Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... with piggyBac transposase (PB210PA-1, System Biosciences) and a piggyBac plasmid expressing SpCas9 under a doxycycline inducible promoter and containing a blasticidin selection cassette ...
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bioRxiv - Immunology 2021Quote: IL-23 EEV(EEV651A-1, System Biosciences) was hydrodynamically injected in a volume of sterile HBSS (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... and HEK293TN cells (System Biosciences, #LV900A-1) were routinely cultured in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Molecular Biology 2023Quote: ... ni piggyBac transposase (PB200PA-1; System Biosciences) (2) ...
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bioRxiv - Microbiology 2023Quote: ... and HR110PA-1 (System Biosciences, CA, USA). The pX330-puro vector was constructed by integrating the puromycin resistance gene sequence from the HR110PA-1 vector into the pX330 vector ...
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bioRxiv - Cancer Biology 2024Quote: ... and 0.5% TransDux (LV860A-1, System Biosciences). 72 hours after transduction ...
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bioRxiv - Developmental Biology 2022Quote: ... with 250 μg protein equivalent of AFSC-EVs stained using 2 μL of ExoGlow™-Vivo (Near IR) EV Labeling Kit (System Biosciences, Palo Alto, CA).
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bioRxiv - Neuroscience 2020Quote: ... and the membrane and intra-vesicular positive EV markers CD81 (1:500; cat. no. EXOAB-CD81A-1, System Biosciences) and alix (0.4 μg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... or a pCDH vector (System Biosciences, CD550A-1). JNK1 (NCBI RefSeq NM_001323302.1 ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293TN cells (System Biosciences, cat. no. LV900A-1) and HeLa cells (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH ...
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bioRxiv - Bioengineering 2021Quote: ... coli Minicircle Strain (System Biosciences, catalog #: MN900A-1) overnight at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... pGreenpuro shRNA cloning and expression lentivector was purchased from System Bioscience (Cat. No. SI505A-1, System Biosciences, LLC, CA). Primers for shRNA are shown in S1 Table ...
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bioRxiv - Developmental Biology 2022Quote: ... using Cold Fusion cloning (System Biosciences, MC010B-1). All constructs were sequence verified for successful insert integration ...
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bioRxiv - Genetics 2022Quote: ... and concentrated using PEG (System Biosciences, LV825A-1) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... dual-reporter lentiviral plasmids pLL410_EF1a-rFLuc-T2A-GFP-mPGK-Puro (LL410PA-1) and pLL-CMV-rFLuc-T2A-GFP-mPGK-Puro (LL310PA-1) were obtained from System Bioscience. Lentiviral generation was carried out as previously described [11] ...
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bioRxiv - Developmental Biology 2020Quote: ... 7 that exhibited distinct spots by transient expression (Supplementary table 1) were subcloned into a piggybac-vector PB510B-1 (System BioSciences) to establish stable lines ...
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bioRxiv - Cell Biology 2020Quote: Lentiviral cloning and expression vectors pCDH-UbC-MCS-EF1-Hygro (CD615B-1) and pCDH-EF1-MCS-T2A-Puro (CD520A-1) were purchased from System Biosciences Inc ...
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bioRxiv - Immunology 2020Quote: To silence miR-424 the GFP tagged lentiviral vectors that express antisense hsa-miR-424 (MZIP424-PA-1) and corresponding scramble control lentiviral vectors (MZIP000-PA-1) were purchased from SBI (System Biosciences). Transfection of 293T packaging cell line was performed using calcium-phosphate protocol and infection of target cells was done as previously described (Platzer et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... for 1 h at room temperature and incubated with primary antibodies overnight at 4 °C with anti-Cd9 ([1:3000; System Biosciences, Palo Alto ...
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bioRxiv - Developmental Biology 2020Quote: ... 4 mL 5× PEG reagent (System Biosciences, LV825A-1) was added and mixed by pipetting ...
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bioRxiv - Biophysics 2022Quote: ... PB-CMV-MCS-EF1α-Puro (System Bioscience #PB510B-1) was linearized using EcoRI and BamHI ...
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bioRxiv - Cell Biology 2021Quote: ... following manufacturer instructions (QuantiMir, System Biosciences, cat. RA670A-1). To acquire the miRNA-enriched RNA fraction from adipose tissue miRNeasy Mini Kit was used (Qiagen ...
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bioRxiv - Immunology 2021Quote: 293TN Producer cell line (System Biosciences, Cat #LV900A-1) was maintained in DMEM containing 10% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... and precipitated with PEG-it (#LV825A-1, System Biosciences). The virus precipitates were resuspended in PBS and stored at −80°C ...
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bioRxiv - Neuroscience 2022Quote: The clonal control iPSC lines SC102A-1 (System Biosciences) and CRTDi011-A (this study) ...
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bioRxiv - Physiology 2023Quote: ... ExoQuick (Cat #EXOQ5A-1, System Biosciences, Palo Alto, CA) was added to the supernatant at a 1:4 ratio for 30 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... TransDux MAX Lentivirus Transduction Enhancer (System Biosciences LV860A-1), and 10 uM Y-27632 and plated as monolayers on Collagen I (Advanced Biomatrix 5010) ...
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bioRxiv - Cancer Biology 2024Quote: ... A water-soluble cumate solution (QM150A-1, System Biosciences) was used to induce PAX3::FOXO1-T2A-mCherry ...
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bioRxiv - Cancer Biology 2021Quote: ... the medium was replaced with DMEM without FBS for 1 hour before the plasmids pCDH-GAL-9-T2A-Puro (System Biosciences, CD527A-1) or pLKO ...
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bioRxiv - Genetics 2023Quote: ... 6 X 105 cells were co-transfected with 5 µg of gRNA-containing piggyBac vector and 1 µg of piggyBac transposase plasmid (System Biosciences PB210PA-1) using Lipofectamine 3000 Transfection Reagent ...
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bioRxiv - Synthetic Biology 2021Quote: ... A total of 4 μg of piggyBac targeting base editor plasmids were transfected with 1 μg of super transposase plasmid (SBI System Biosciences cat# PB210PA-1) using Lipofectamine 3000 following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... E0771 and RM-1 cells were transduced with lentiviral vectors carrying the luciferase expression cassette with TransDux MAXTM (System Bioscience, catalog number: LV860A-1). Luciferase-labeled E0771 and RM-1 cells were then selected in the presence of 300 µg/mL hygromycin ...
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bioRxiv - Cancer Biology 2023Quote: ... omentum tissues were minced with sterile razor blades and approximately 0.5 g of minced tissue was cultured per 100 mm tissue culture dish in 10 mL DMEM/F12 media supplemented with 1% exosome-depleted FBS (EXO-FBS-250A-1, System Biosciences, Palo Alto, CA). The following day ...
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bioRxiv - Genetics 2021Quote: ... This plasmid was derived from the MN530A-1 (System Biosciences) by replacing the GFP Reporter sequence with a multiple cloning site using the restriction enzymes XmaI and StuI and duplexed donor oligonucleotides #2020 and #2021 (Supplemental Table 1) ...
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bioRxiv - Developmental Biology 2022Quote: Lentivirus was produced in 293TN cells (System Bioscience, LV900A-1) expanded in DMEM with 10% FBS as previously described (Beronja et al. ...
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bioRxiv - Immunology 2021Quote: ... 8×106 293TN Producer cells (System Biosciences, Cat # LV900A-1) were plated in T150 flasks overnight ...
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bioRxiv - Immunology 2021Quote: ... 55 mL of PureFection reagent (System Biosciences; Cat # LV750A-1) was added to each 1mL tube ...
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bioRxiv - Bioengineering 2022Quote: ... 0.5μg of Super PiggyBac Transposase (System Biosciences, PB210PA-1-SBI) and 0.5μg of piRFP670-N1 (addgene #45457 ...
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bioRxiv - Cell Biology 2022Quote: ... with a PiggyBac transposon expression vector (PB210PA-1; System Biosciences), or pEGFP-C1-FLAG-Ku80 ((Britton et al. ...
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bioRxiv - Synthetic Biology 2022Quote: ... or PiggyBac Transposon vector plasmid (System Biosciences, Cat # PB510B-1). Plasmids encoding 2nd-generation packaging plasmids (psPAX2 – Cat #12260 ...
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bioRxiv - Physiology 2023Quote: ... and transfected with CD81-GFP (System Biosciences # CYTO124-PA-1) and mPlum-mito-3 (Addgene plasmid #55988 ...
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bioRxiv - Neuroscience 2022Quote: ... The Super PiggyBac Transposase plasmid (System Biosciences; Cat# PB210PA-1) was obtained from BioCat ...
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bioRxiv - Biochemistry 2023Quote: The PiggyBac Single Promotor vector (cat# PB531A-1, System Biosciences) was modified in house to replace the gene encoding the red fluorescent protein (RFP ...
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bioRxiv - Neuroscience 2023Quote: ... into PB-CMV-MCS-EF1-Puro (System Biosciences #PB510B-1).
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bioRxiv - Neuroscience 2023Quote: ... into PB-CMV-MCS-EF1-Puro (System Biosciences #PB510B-1).
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bioRxiv - Neuroscience 2023Quote: ... into PB-CMV-MCS-EF1-Puro (System Biosciences #PB510B-1).
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bioRxiv - Genetics 2023Quote: ... Super PiggyBac Transposase (PB210PA-1) was purchased from System Biosciences.
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bioRxiv - Cancer Biology 2024Quote: ... 20% TransDux MAX Lentivirus Transduction Enhancer (LV860A-1, System Biosciences), and 0.5% TransDux (LV860A-1 ...
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bioRxiv - Biochemistry 2021Quote: ... coli minicircle producer strain24 competent cells (Cat. No. MN900A-1) and Arabinose Induction Solution (Cat. No. MN850A-1) were purchased from System Biosciences (Palo Alto, CA, USA). The luciferase-expressing ...
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bioRxiv - Molecular Biology 2020Quote: ... exosome-depleted FBS (cat. no. EXO-FBS-50A-1, System Biosciences) was added to the cells ...