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Citations for System Biosciences :
1 - 50 of 67 citations for Cow Ataxin 10 ATXN10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The number of CD63+ EVs collected in the EV-enriched fractions were estimated by ELISA (System Biosciences). The ELISA standards provided in the kit are calibrated by NTA to measure the number of exosomes and establish a standard curve based on exosome abundance ...
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bioRxiv - Cell Biology 2021Quote: ... we seeded cells at ∼30% confluency in exosome-depleted medium that was obtained either by ultracentrifugation of DMEM plus 10% FBS or DMEM supplemented with 10% exosome-depleted FBS (System Biosciences, Palo Alto, CA) in 150 mm CellBIND tissue culture dishes (Corning ...
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bioRxiv - Microbiology 2022Quote: ... using the Multiplex gRNA kit (System Biosciences) according to the manufacturer protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... using the Cold Fusion kit (System Biosciences Inc) according to manufacturer instructions ...
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bioRxiv - Biochemistry 2019Quote: ... cells were seeded at ~10% confluency in exosome-depleted medium (System Biosciences, Palo Alto, CA) in 150mm CellBIND tissue culture dishes (Corning ...
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bioRxiv - Molecular Biology 2021Quote: ... EVs were isolated from tissue culture media using a commercial kit containing a polyethylene glycol (PEG) solution (ExoQuick-TC ULTRA kit, EQULTRA-20TC-1; System Biosciences) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... in DMEM medium supplemented with 10% FBS depleted of exosomes (System Biosciences, EXO-FBS-250A-1). Cells were maintained in this medium for 24 h before supernatant collection for EV purification ...
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bioRxiv - Bioengineering 2023Quote: ... and the pPACKH1 HIV Lentivector Packaging Kit (System Biosciences) was used to produce lentivirus particles ...
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bioRxiv - Genetics 2021Quote: ... along with 1/10 total plasmid mass of Super PiggyBac Transposase Expression Vector (System Bioscience, Inc. #PB210PA-1). Stable integrants were selected with 1-2 μg/mL puromycin.
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bioRxiv - Molecular Biology 2022Quote: ... Colonies were stained using an Alkaline Phosphatase Assay Kit (System Biosciences) and evaluated under an Olympus CK2 microscope.
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bioRxiv - Neuroscience 2021Quote: ... Exosomes were extracted from supernatant with an ExoQuick kit (System Biosciences). The presence of exosomal markers and size distribution of the particles was confirmed previously [22] ...
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bioRxiv - Biochemistry 2021Quote: ... or ExoQuick ULTRA EV Isolation Kit for Serum and Plasma (System Biosciences), and protocols were performed according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Microvesicles were isolated using ExoQuick-TC kit (System Biosciences, Palo Alto, USA) accordingly to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... samples were purified using the CD9 Exo-Flow Capture Kit (System Biosciences) using the manufacturer-provided protocol ...
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bioRxiv - Cancer Biology 2019Quote: Cells were cultured in DMEM/1640 supplemented with 10% exosome-depleted FBS (EXO-FBS-250A-1, SBI System Biosciences, USA) using 10×10 cm2 disks (1×106 cells per disk ...
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bioRxiv - Molecular Biology 2023Quote: ... U2-OS cells (UC Berkeley BDS Cell Culture Facility) cultured in DMEM (1 g/L glucose, 10% FBS) were transfected with a modified PiggyBac Transposase plasmid (System Biosciences) and a PGK.mNeonGreen-LacI.NLS.IRES.Puro integration plasmid using Fugene6 transfection reagent ...
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bioRxiv - Neuroscience 2024Quote: ... and the medium was changed to the same DMEM but supplemented with 10% exosome-depleted FBS (EXO-FBS-50A-1, System Bioscience). The media was collected on the day of isolation ...
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bioRxiv - Developmental Biology 2022Quote: AFSC-EVs were fluorescently labelled using ExoGlow-EV labeling kit (SBI System Biosciences), using the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... concentrated by PEG-it™ precipitation kits from System Bioscience (Palo Alto, CA), were re-suspended in cold PBS and stored at −80 °C.
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bioRxiv - Developmental Biology 2022Quote: ... pre-digested with XhoI and HindIII using Cold Fusion Cloning kit (System Biosciences). Deletion of putative TF binding sites from those enhancers were performed using QuickChange II XL site-directed mutagenesis kit (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2024Quote: ... we utilized the MC-Easy™ Minicircle DNA Production Kit (System Bioscience, SBI). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... EVs were quantified with the ExoELISA Complete Kit (CD63 Detection, System Biosciences, USA) according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2024Quote: ... Extracellular vesicles were isolated from 10 mL of cell culture supernatant using the ExoQuick-TC® Exosome Precipitation Solution (cat# EXOTC10A-1; System Biosciences) according to the manufacturer’s instructions with a few modifications ...
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bioRxiv - Genetics 2019Quote: PCR amplification was performed according to the PrecisionX Multiplex gRNA Cloning Kit (System Bioscience). BbsI-linearized piggyBac-U6 backbone was mixed with purified PCE product in a vector:insert 2:1 ratio and was directly used for transformation of One Shot MAX Efficiency DH5α-T1 Competent Cells (Invitrogen).
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bioRxiv - Cell Biology 2021Quote: ... and the rcccDNA was isolated using the MC-Easy minicircle production kit (System Biosciences) according to the manufacturer’s recommendations.
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bioRxiv - Immunology 2019Quote: ... The sgRNA transcripts were generated by IVT synthesis kit (System Biosciences, Palo Alto, CA). Quality of sgRNA transcripts was analyzed by NanoDrop (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: EV RNA was isolated with a SeraMir Exosome RNA Column Purification Kit (System Biosciences) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Exosomes were labeled with ExoGlow™-Vivo EV Labeling Kit (System Biosciences, Palo Alto, CA). Labeled exosomes or PBS containing exosomes were injected via tail vein into 17-18-month-old C57BL/6 mice ...
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bioRxiv - Developmental Biology 2019Quote: ... Exosomes were then collected following the manufacturer’s instructions for the ExoQuick™kit (System Biosciences). For Western blot analysis ...
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bioRxiv - Microbiology 2020Quote: ... Fusion was performed with the Cold Fusion Cloning Kit (SBI System Biosciences, Cat. #MC010B-1).
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bioRxiv - Cell Biology 2021Quote: Inducible Cas9 iPSCs were generated using an AAVS1 TALE-Nuclease KIT (GE60xA-1; System Biosciences) and an iCas9 HR donor plasmid containing a TRE3G promoter-driven spCas9 with a C-terminal MYC NLS followed by a CMV promoter-driven Tet-On-3G and a neomycin resistance adapted from Ihry et al ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid was then packaged into viral particles using the pPACKH1 lentivector Packaging Kit (System Bioscience) in HEK293T cells ...
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bioRxiv - Bioengineering 2023Quote: ... Then MC DNA was produced and purified by using an MC-EsayTM Kit (System Biosciences), without the optional dNTP removal step.
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bioRxiv - Developmental Biology 2023Quote: ... or goat anti-rabbit (1:20,000; EXOAB-KIT-1; System Biosciences, Palo Alto, CA, USA). Protein signals were detected using the SuperSignal West Femto Chemiluminescent kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... omentum tissues were minced with sterile razor blades and approximately 0.5 g of minced tissue was cultured per 100 mm tissue culture dish in 10 mL DMEM/F12 media supplemented with 1% exosome-depleted FBS (EXO-FBS-250A-1, System Biosciences, Palo Alto, CA). The following day ...
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bioRxiv - Molecular Biology 2021Quote: ... exosomes from ASCs were labeled fluorescent red using an ExoGlow-Protein EV Labeling Kit (System Biosciences) and then incubated with precipitation solution at 4°C overnight ...
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bioRxiv - Neuroscience 2019Quote: ... miRNA was converted to cDNA using the QuantiMiR reverse transcription kit (System Biosciences, Mountain View, CA). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... or by labeling RNA cargos by using the ExoGlow-RNATM EV Labeling Kit (System Biosciences, Inc.) following the manufacturers’ recommendations ...
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bioRxiv - Zoology 2023Quote: ... EVs were isolated from the resulting supernatant using the ExoQuick Exosome Isolation kit from System Biosciences following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: Western blotting was performed using ExoA Antibody Kit according to the manufacturer’s protocol (System Biosciences, USA). Immunoblots were cut prior to primary antibody hybridization ...
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bioRxiv - Biochemistry 2021Quote: MDA-MB-231 or HEK 293T cells were grown to ~80% confluency in 14 × 150 mm dishes with 10% exosome-depleted FBS (Exo-FBS from System Biosciences, EXO-FBS-250A-1) in DMEM GlutaMAX media ...
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bioRxiv - Neuroscience 2022Quote: NDE were isolated from total EVs of plasma using the Basic Exo-Flow Capture Kit (System Biosciences) as described (Winston et al. ...
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bioRxiv - Bioengineering 2023Quote: ... OMVs in the total supernatant fraction were extracted using the ExoBacteria™ OMV Isolation Kit (System Biosciences) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... and anti-rabbit HRP-linked secondary antibody (EXOAB-KIT-1) were purchased from System Biosciences (CA, USA). Anti-beta-actin antibody (sc-47778) ...
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bioRxiv - Developmental Biology 2020Quote: ... ESC were washed twice with PBS and incubated with AP Staining kit™ (Cat # AP100R-1, System Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... and the EVs were isolated with a commercially available precipitation kit (ExoQuick, System Biosciences, Palo Alto, CA, US) following the protocol provided by the vendor ...
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bioRxiv - Bioengineering 2021Quote: ... Quantification of EV release was accomplished using an ExoELISA-ULTRA Complete Kit (CD63 Detection; System Biosciences, Mountain View, CA). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... The cDNA was subsequently cloned into the pLVX tight puro vector using the Cold FusionTM cloning kit (System Biosciences), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... was added to a tube along with purefection reagent and pPackF1 lentiviral packaging kit plasmids (System Biosciences, # LV100A-1) and either PCF1 (empty vector control) ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... Quantification of successfully integrated piggyBac transposons was performed using a piggyBac qPCR copy number kit (System Biosciences, PBC100A-1) according to the manufacturer’s instructions.