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Citations for System Biosciences :
101 - 150 of 627 citations for Cell Isolation Optimizing System since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... into XPack CMV constructs (System Biosciences). For cyclin D1 overexpression ...
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bioRxiv - Cell Biology 2022Quote: ... cumate (30 ug/ml; System Biosciences) was added in the media immediately after the mitotic shake-off and was washed out prior to imaging ...
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bioRxiv - Bioengineering 2021Quote: ... and anti-CD81 from System Biosciences. Wheat Germ Agglutinin (WGA ...
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bioRxiv - Immunology 2023Quote: ... with 1x Cumate solution (System Biosciences) to induce protein expression ...
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bioRxiv - Developmental Biology 2023Quote: ... with Super PiggyBac Transposase (System Biosciences) and the PiggyBac transposon vector with human GATA6-2A-EGFP under control of the tetracycline responsive element promoter ...
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bioRxiv - Developmental Biology 2023Quote: ... with Super PiggyBac Transposase (System Biosciences) and the PiggyBac transposon vector with hGATA6-2A-EGFP under control of the tetracycline responsive element promoter ...
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bioRxiv - Immunology 2023Quote: ... into pCDH533-IRES-Neo (System Bioscience). For pCDHTRE-K6a-mCherry ...
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bioRxiv - Cancer Biology 2023Quote: ... a PEO1-OR-dCas9-KRAB-MeCP2 stable line was first generated using the PiggyBac system (System Bioscience). The PiggyBac Donor plasmid ...
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bioRxiv - Microbiology 2021Quote: ... EBV-negative Akata(-) cells were treated with purified exosomes in RPMI1640 supplemented with 5% exosome-depleted FBS (System Biosciences, Palo Alto, CA, USA). After 6 h ...
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bioRxiv - Molecular Biology 2023Quote: ... in which A375 Cas9 cells were co-transfected with the rescue plasmids as well as the Super PiggyBac Transposase Expression Vector (System Bioscience Inc, PB200PA) at a 4:1 ratio utilising Lipofectamine™ LTX reagents (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... 6 X 105 cells were co-transfected with 5 µg of gRNA-containing piggyBac vector and 1 µg of piggyBac transposase plasmid (System Biosciences PB210PA-1) using Lipofectamine 3000 Transfection Reagent ...
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bioRxiv - Immunology 2021Quote: ... concentrated using Peg-it solution (System Biosciences) and titrated on HEK293T cells ...
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bioRxiv - Cell Biology 2022Quote: ... Stock cumate solution (System Biosciences # QM100A-1) was diluted to 5X in culture medium prior to use.
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bioRxiv - Bioengineering 2020Quote: ... coli (System Biosciences, Palo Alto, CA, USA) were transformed with the original parental plasmids (PP ...
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bioRxiv - Cell Biology 2021Quote: ... with piggyBac transposase (PB210PA-1, System Biosciences) and a piggyBac plasmid expressing SpCas9 under a doxycycline inducible promoter and containing a blasticidin selection cassette ...
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bioRxiv - Biophysics 2020Quote: ... into the lentiviral vector CD813A (System Biosciences) encoding the 5’ untranslated region of human β-globin (HBB 5’-UTR).
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bioRxiv - Bioengineering 2022Quote: ... 100 µL of ExoQuick-TC (System Biosciences) was added ...
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bioRxiv - Genomics 2019Quote: ... Cas9 protein was bought from System Biosciences (2438 Embarcadero Way ...
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bioRxiv - Cell Biology 2019Quote: ... Phenol-free lysis buffer (SeraMir, System Biosciences) was added to pellets ...
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bioRxiv - Cancer Biology 2019Quote: ... and anti-CD81 antibodies (System Biosciences, CA).
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bioRxiv - Biochemistry 2021Quote: ... pCDH-CMV-MCS-EF1-Puro (System Biosciences) for the viral infection and expression of N-terminal FLAG-tagged USP8 ...
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bioRxiv - Immunology 2021Quote: ... pCDH-CMV-MCS-EF1α-RFP (System Biosciences). To facilitate the secretion and purification of the protein ...
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bioRxiv - Immunology 2021Quote: IL-23 EEV(EEV651A-1, System Biosciences) was hydrodynamically injected in a volume of sterile HBSS (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... using the Multiplex gRNA kit (System Biosciences) according to the manufacturer protocol ...
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bioRxiv - Bioengineering 2023Quote: ... LVVs were stained with ExoGlow (System Biosciences), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... ni piggyBac transposase (PB200PA-1; System Biosciences) (2) ...
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bioRxiv - Microbiology 2023Quote: ... and HR110PA-1 (System Biosciences, CA, USA). The pX330-puro vector was constructed by integrating the puromycin resistance gene sequence from the HR110PA-1 vector into the pX330 vector ...
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bioRxiv - Immunology 2024Quote: ... and Transposase Expression vector DNA (System Biosciences). Cells were cultured with 2 µg/mL puromycin (Life technologies) ...
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bioRxiv - Cancer Biology 2024Quote: ... and 0.5% TransDux (LV860A-1, System Biosciences). 72 hours after transduction ...
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bioRxiv - Immunology 2024Quote: ... and pCDH-EF1α-T2A-copGFP (System Biosciences) for expression in HEK293T or tagged with FLAG-HA then cloned into the pUC57-mini vector (synthesized by Genscript ...
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bioRxiv - Cell Biology 2022Quote: Bare 293T cells were first co-transfected using PEI47 with the H2B-mTagBFP2 vector and Super PiggyBac Transposase-expressing vector (System Biosciences Inc. # PB210PA-1) via polyethylenimine (Sigma #408727-100mL ...
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bioRxiv - Cancer Biology 2020Quote: ... MDA-MB-231 and MDA-MB-231-Luc+ cells were transduced with lentivirus vector expressing a GFP or RFP tag fused to the membrane-associated tetraspanin CD63 (System Biosciences, Palo Alto, CA). Breast cancer cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cell Biology 2022Quote: ... IL) lentiviruses were grown in human embryonic kidney 293T cells and concentrated using the PEG-it Virus Precipitation Solution (System Biosciences, Mountain View, CA), as previously described (51) ...
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bioRxiv - Cancer Biology 2023Quote: ... E0771 and RM-1 cells were transduced with lentiviral vectors carrying the luciferase expression cassette with TransDux MAXTM (System Bioscience, catalog number: LV860A-1). Luciferase-labeled E0771 and RM-1 cells were then selected in the presence of 300 µg/mL hygromycin ...
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bioRxiv - Biochemistry 2021Quote: ... coli minicircle producer strain24 competent cells (Cat. No. MN900A-1) and Arabinose Induction Solution (Cat. No. MN850A-1) were purchased from System Biosciences (Palo Alto, CA, USA). The luciferase-expressing ...
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bioRxiv - Biochemistry 2021Quote: MDA-MB-231 or HEK 293T cells were grown to ~80% confluency in 14 × 150 mm dishes with 10% exosome-depleted FBS (Exo-FBS from System Biosciences, EXO-FBS-250A-1) in DMEM GlutaMAX media ...
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bioRxiv - Cell Biology 2020Quote: ... along with PiggyBac Transposase expression plasmid (System Biosciences) and selected with 500 μg/ml G418 (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... or a pCDH vector (System Biosciences, CD550A-1). JNK1 (NCBI RefSeq NM_001323302.1 ...
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bioRxiv - Cancer Biology 2021Quote: ... or pGreenFire1-SP1 (System Biosciences; Palo Alto, CA) and pRL-TK vector (as an inner control that contains Renilla luciferase sequences (Promega ...
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bioRxiv - Microbiology 2021Quote: ... we follow the exoQuick-Tc (System Biosciences, USA) standard operating procedure to extract exosomes from the cell supernatant ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-CD63 (System Bioscience, Mountain View, CA, USA), anti-AIP1/Alix (BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... EVs were isolated using ExoQuick-TC (System Biosciences) according to manufacturers’ instructions ...
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Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... based on a pCDH derived vector (System Biosciences) so that mtagBFP ...
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bioRxiv - Microbiology 2021Quote: ... The lentiviral vector pCDH-EF1a-GFP (System Bioscience), the packaging plasmid psPAXII (Addgene) ...
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bioRxiv - Bioengineering 2021Quote: ... coli Minicircle Strain (System Biosciences, catalog #: MN900A-1) overnight at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... pGreenpuro shRNA cloning and expression lentivector was purchased from System Bioscience (Cat. No. SI505A-1, System Biosciences, LLC, CA). Primers for shRNA are shown in S1 Table ...
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bioRxiv - Genomics 2020Quote: ... and concentrated using PEG-it (SBI System Biosciences) according to the manufacturer’s recommendations.
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bioRxiv - Developmental Biology 2020Quote: ... scFv-sfGFP was cloned into PB533A (System Biosciences) and transfected with the transposase expression vector (PB200PA-1 ...
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bioRxiv - Genetics 2022Quote: ... Supernatants were purified using PEG-it (System Biosciences); concentrated lentivirus was stored at −80°C.
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bioRxiv - Developmental Biology 2022Quote: ... using Cold Fusion cloning (System Biosciences, MC010B-1). All constructs were sequence verified for successful insert integration ...