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Citations for System Biosciences :
1 - 50 of 74 citations for Arg8 Vasopressin AVP Chemiluminescent ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The number of CD63+ EVs collected in the EV-enriched fractions were estimated by ELISA (System Biosciences). The ELISA standards provided in the kit are calibrated by NTA to measure the number of exosomes and establish a standard curve based on exosome abundance ...
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bioRxiv - Molecular Biology 2021Quote: ... with 1/5 final dilution (System Biosciences, LV810A-1), and the mixture was incubated at 4 °C for 6 hrs ...
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bioRxiv - Developmental Biology 2020Quote: ... 4 mL 5× PEG reagent (System Biosciences, LV825A-1) was added and mixed by pipetting ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Virus was concentrated using 5× PEG- it™ (System Biosciences) as per manufacturer’s protocol and virus pellet obtained was aliquoted in opti-MEM and stored at -80°C ...
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bioRxiv - Cell Biology 2023Quote: ... and 5 ng μL-1 Cas9 mRNA (System Biosciences, Palo Alto, CA) in 10 mM Tris-HCl pH 8.0 and 0.1 mM EDTA were used for injection into the pronucleus of fertilized mouse zygotes ...
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bioRxiv - Genetics 2019Quote: ... matched with Zfr knockdown: 5’-AACCGATGTACTTCCCGTTAAT) were cloned into the pGreenPuro backbone from System Biosciences. This construct was used to produce a 6-well of lentivirus according to standard protocols in 293T cells using the third-generation system and concentrated 1:100 with lenti-X (Clontec) ...
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bioRxiv - Physiology 2023Quote: ... and 5 µL of de-fibrination reagent (Cat #TMEXO-1, System Biosciences, Palo Alto, CA) was added to the plasma to remove clotting factors prior to the isolation of EVs ...
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bioRxiv - Developmental Biology 2022Quote: ... The gRNA (5 pg/embryo) and mRNA encoding Cas9 protein (150 pg/embryo, System Biosciences) were injected into one-cell stage embryos ...
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bioRxiv - Microbiology 2021Quote: ... ARN8 HBEGFKO cells were transfected at 80% confluence in a 6-well plate with pCMV-hHBEGF (2 µg) and PB Transposase vector (0.5 µg) (System Biosciences) using FuGENE® HD (Promega ...
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bioRxiv - Genetics 2021Quote: ... An in vitro transcribed sgRNA targeting Slc16a11 (5’-AGTCCTAACCTCGCTTGGCT-3’) and hspCas9 mRNA (CAS500A-1, System Biosciences) were microinjected into C57BL/6J mouse zygotes by the Genome Modification Facility at Harvard University ...
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bioRxiv - Microbiology 2021Quote: ... media were changed to DMEM supplemented with 5% exosome-depleted FBS (System Biosciences, Palo Alto, CA, USA). After 48 h ...
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bioRxiv - Microbiology 2022Quote: ... using the Multiplex gRNA kit (System Biosciences) according to the manufacturer protocol ...
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bioRxiv - Neuroscience 2022Quote: ... 500 μL of human plasma were incubated with 5 μL purified thrombin (System Biosciences, Inc.; Catalog # TMEXO-1) at room temperature for 5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... using the Cold Fusion kit (System Biosciences Inc) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... EVs were isolated from tissue culture media using a commercial kit containing a polyethylene glycol (PEG) solution (ExoQuick-TC ULTRA kit, EQULTRA-20TC-1; System Biosciences) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: ... and the pPACKH1 HIV Lentivector Packaging Kit (System Biosciences) was used to produce lentivirus particles ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was blocked in freshly prepared 5% BSA diluted in TBS before being immunoblotted with specific primary antibodies (CD81; #EXOAB-CD81A-1 System Biosciences, GM130 ...
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bioRxiv - Molecular Biology 2022Quote: ... Colonies were stained using an Alkaline Phosphatase Assay Kit (System Biosciences) and evaluated under an Olympus CK2 microscope.
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bioRxiv - Neuroscience 2021Quote: ... Exosomes were extracted from supernatant with an ExoQuick kit (System Biosciences). The presence of exosomal markers and size distribution of the particles was confirmed previously [22] ...
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bioRxiv - Microbiology 2021Quote: ... EBV-negative Akata(-) cells were treated with purified exosomes in RPMI1640 supplemented with 5% exosome-depleted FBS (System Biosciences, Palo Alto, CA, USA). After 6 h ...
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bioRxiv - Neuroscience 2020Quote: ... fibrinogen and fibrin proteins known to impede efficient EV recovery were removed using 5 U/mL of thrombin (System Biosciences, Inc.; Mountainview, CA) for 30 minutes at RT followed by addition of 495 μL of Dulbecco’s PBS-1X (DPBS ...
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bioRxiv - Molecular Biology 2023Quote: ... a minimal piggyBac cargo construct was created by deleting all intervening sequences between the 5’ and 3’ terminal repeats (including core insulators) of the PB-CMV-MCS-EF1α-Puro vector (System Biosciences, PB510B-1). A gBlock (Integrated DNA Technologies ...
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bioRxiv - Genetics 2023Quote: ... 6 X 105 cells were co-transfected with 5 µg of gRNA-containing piggyBac vector and 1 µg of piggyBac transposase plasmid (System Biosciences PB210PA-1) using Lipofectamine 3000 Transfection Reagent ...
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bioRxiv - Biochemistry 2021Quote: ... or ExoQuick ULTRA EV Isolation Kit for Serum and Plasma (System Biosciences), and protocols were performed according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Microvesicles were isolated using ExoQuick-TC kit (System Biosciences, Palo Alto, USA) accordingly to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... samples were purified using the CD9 Exo-Flow Capture Kit (System Biosciences) using the manufacturer-provided protocol ...
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bioRxiv - Developmental Biology 2022Quote: AFSC-EVs were fluorescently labelled using ExoGlow-EV labeling kit (SBI System Biosciences), using the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... concentrated by PEG-it™ precipitation kits from System Bioscience (Palo Alto, CA), were re-suspended in cold PBS and stored at −80 °C.
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bioRxiv - Developmental Biology 2022Quote: ... pre-digested with XhoI and HindIII using Cold Fusion Cloning kit (System Biosciences). Deletion of putative TF binding sites from those enhancers were performed using QuickChange II XL site-directed mutagenesis kit (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2024Quote: ... we utilized the MC-Easy™ Minicircle DNA Production Kit (System Bioscience, SBI). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... EVs were quantified with the ExoELISA Complete Kit (CD63 Detection, System Biosciences, USA) according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2021Quote: ... Virus-containing supernatants were either collected or concentrated by mixing with quarter volumes of 5 × PEG-it Virus Precipitation Solution (System Biosciences, Palo Alto, CA, United States), followed by centrifugation according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: PCR amplification was performed according to the PrecisionX Multiplex gRNA Cloning Kit (System Bioscience). BbsI-linearized piggyBac-U6 backbone was mixed with purified PCE product in a vector:insert 2:1 ratio and was directly used for transformation of One Shot MAX Efficiency DH5α-T1 Competent Cells (Invitrogen).
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bioRxiv - Cell Biology 2021Quote: ... and the rcccDNA was isolated using the MC-Easy minicircle production kit (System Biosciences) according to the manufacturer’s recommendations.
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bioRxiv - Immunology 2019Quote: ... The sgRNA transcripts were generated by IVT synthesis kit (System Biosciences, Palo Alto, CA). Quality of sgRNA transcripts was analyzed by NanoDrop (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: EV RNA was isolated with a SeraMir Exosome RNA Column Purification Kit (System Biosciences) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Exosomes were labeled with ExoGlow™-Vivo EV Labeling Kit (System Biosciences, Palo Alto, CA). Labeled exosomes or PBS containing exosomes were injected via tail vein into 17-18-month-old C57BL/6 mice ...
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bioRxiv - Developmental Biology 2019Quote: ... Exosomes were then collected following the manufacturer’s instructions for the ExoQuick™kit (System Biosciences). For Western blot analysis ...
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bioRxiv - Microbiology 2020Quote: ... Fusion was performed with the Cold Fusion Cloning Kit (SBI System Biosciences, Cat. #MC010B-1).
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bioRxiv - Cell Biology 2021Quote: Inducible Cas9 iPSCs were generated using an AAVS1 TALE-Nuclease KIT (GE60xA-1; System Biosciences) and an iCas9 HR donor plasmid containing a TRE3G promoter-driven spCas9 with a C-terminal MYC NLS followed by a CMV promoter-driven Tet-On-3G and a neomycin resistance adapted from Ihry et al ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid was then packaged into viral particles using the pPACKH1 lentivector Packaging Kit (System Bioscience) in HEK293T cells ...
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bioRxiv - Bioengineering 2023Quote: ... Then MC DNA was produced and purified by using an MC-EsayTM Kit (System Biosciences), without the optional dNTP removal step.
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bioRxiv - Developmental Biology 2023Quote: ... or goat anti-rabbit (1:20,000; EXOAB-KIT-1; System Biosciences, Palo Alto, CA, USA). Protein signals were detected using the SuperSignal West Femto Chemiluminescent kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... exosomes from ASCs were labeled fluorescent red using an ExoGlow-Protein EV Labeling Kit (System Biosciences) and then incubated with precipitation solution at 4°C overnight ...
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bioRxiv - Neuroscience 2019Quote: ... miRNA was converted to cDNA using the QuantiMiR reverse transcription kit (System Biosciences, Mountain View, CA). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... or by labeling RNA cargos by using the ExoGlow-RNATM EV Labeling Kit (System Biosciences, Inc.) following the manufacturers’ recommendations ...
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bioRxiv - Zoology 2023Quote: ... EVs were isolated from the resulting supernatant using the ExoQuick Exosome Isolation kit from System Biosciences following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: Western blotting was performed using ExoA Antibody Kit according to the manufacturer’s protocol (System Biosciences, USA). Immunoblots were cut prior to primary antibody hybridization ...
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bioRxiv - Neuroscience 2022Quote: NDE were isolated from total EVs of plasma using the Basic Exo-Flow Capture Kit (System Biosciences) as described (Winston et al. ...
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bioRxiv - Bioengineering 2023Quote: ... OMVs in the total supernatant fraction were extracted using the ExoBacteria™ OMV Isolation Kit (System Biosciences) according to the manufacturer’s protocol ...