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Citations for System Biosciences :
151 - 200 of 260 citations for 8 Chloro 2 3 4 5 tetrahydropyrido 3 2 f 1 4 oxazepine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Fusion was performed with the Cold Fusion Cloning Kit (SBI System Biosciences, Cat. #MC010B-1).
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bioRxiv - Cell Biology 2021Quote: Inducible Cas9 iPSCs were generated using an AAVS1 TALE-Nuclease KIT (GE60xA-1; System Biosciences) and an iCas9 HR donor plasmid containing a TRE3G promoter-driven spCas9 with a C-terminal MYC NLS followed by a CMV promoter-driven Tet-On-3G and a neomycin resistance adapted from Ihry et al ...
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bioRxiv - Cell Biology 2023Quote: ... from 3T3-L1 genomic DNA and cloned into the HR110-PA-1 vector (System Biosciences). Primer sequences to amplify the 5′- and 3′-side genomic fragments for homologous recombination are as follows ...
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bioRxiv - Cancer Biology 2023Quote: 2.5×106 IFU/ml of pooled lenti-miRNA lentivirus library (System Biosciences Cat. #PMIRHPLVHT-1) was mixed with 4 mg/mL polybrene (Sigma ...
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bioRxiv - Immunology 2024Quote: ... The PCR fragments were cloned into a PiggyBac mammalian expression vector (System Biosciences, PB210PA-1), modified to harbor a Secrecon-AA signal peptide111 for secretion at the N-terminus ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified viral particles were transduced into MIN6 cells using TransDux MAX (LV860A-1; System Biosciences) and cultured for 48-72 hours in complete DMEM (11965-092 ...
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bioRxiv - Systems Biology 2020Quote: ... GFP gene was amplified from pGreenPuro shRNA Cloning and Expression Lentivector (CMV; System biosciences, SI505A-1) and sub-cloned into ITR-CAG-DEST-IRES-Neomycin-ITR plasmid (generous gift from Marc Supprian Schmidt) ...
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bioRxiv - Neuroscience 2021Quote: ... The viral particles were precipitated from the media using AAVanced concentration reagent (System Biosciences #AAV100A-1) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... in DMEM medium supplemented with 10% FBS depleted of exosomes (System Biosciences, EXO-FBS-250A-1). Cells were maintained in this medium for 24 h before supernatant collection for EV purification ...
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bioRxiv - Immunology 2021Quote: ... A PEG-it Virus Precipitation Solution (Cat# LV810A-1, System Biosciences, Palo Alto, CA 94303, USA) was added to 40ml of virus culture at a 1:4 ratio ...
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bioRxiv - Cancer Biology 2022Quote: ... Media containing virus particles was mixed with PEG-it virus precipitation solution (System Biosciences, #LV810A-1) and incubated at 4°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... These constructs are based on an empty parent minicircle cloning vector (MN100A-1; System Biosciences (SBI), Palo Alto ...
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bioRxiv - Neuroscience 2022Quote: ... supernatants were incubated with 120 μL ExoQuick Exosome Precipitation solution (System Biosciences, Inc.; Catalog # EXOQ5TM-1) for 30 min at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... The CRISPR gRNA (5pg per embryo) and Cas9 mRNA (150pg per embryo, System Biosciences CAS500A-1) were injected into single-cell embryos ...
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bioRxiv - Molecular Biology 2023Quote: ... the SpyCas9-ABE-tracrRNA PiggyBac transposon plasmid and PiggyBac transposase expression plasmid (System Biosciences PB210PA-1) were mixed in a 2:1 molar ratio and transfected into the HEK293T-dGFP reporter cell line using Lipofectamine 3000 (ThermoFisher L3000015) ...
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bioRxiv - Cancer Biology 2023Quote: EVs from biological fluids were isolated using ExoQuick exosome precipitation solution (System Biosciences, SBI-EXOQ5A-1). Plasma and serum samples were centrifuged at 3,000 × g for 15 min to remove cells and cell debris ...
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bioRxiv - Cancer Biology 2023Quote: ... A second ESR1-DAB2 plasmid in a pCDH-CMV-MCS-ER1α-Puro (System Biosciences #CD510B-1) was received as a kind gift from Dr ...
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bioRxiv - Molecular Biology 2024Quote: ... Lentiviruses were generated by transfecting the plasmids using pPACKH1 Packaging Plasmid Mix (LV500A-1; System Biosciences) into a 293T cell line (LV900A-1 ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... cells were transfected with 0.2 μg of Super PiggyBac Transposase Expression Vector (System Biosciences, PB210PA-1) and 0.2 ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... We created the PB transposase plasmid pSPBase by removing the ccdB gene from PB210PA-1 (System Biosciences) to render it generally propagable ...
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bioRxiv - Cell Biology 2021Quote: ... MNT1 cells were transfected with Pb-TRE3G-SpCas9-IRES-Blast and piggyBac transposase (PB210PA-1, System Biosciences) in 1:3 ratio using lipofectamine (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... cDNA sequences were cloned into the PB-CMV-MCS-EF1α-Puro vector (PB510B-1, SBI System Biosciences) where the CMV promoter was replaced by a PGK promoter ...
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bioRxiv - Genomics 2021Quote: ... was stably introduced in HEK293T cells using the piggy-transposase system (System Biosciences, cat. no. PB210PA-1) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... the gfp-lem8 fusion was inserted into pCDH-CMV-MCS-EF1a-Puro (System Biosciences, cat# CD510B-1). The plasmids were co-transfected with pMD2.G (gift from Dr ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: We used the human iPSC lines mciPS (model no. SC301A-1; System Biosciences, Palo Alto, CA, USA), SBAD2 [75] ...
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bioRxiv - Developmental Biology 2022Quote: ... freshly dissociated brain cells were first transduced with lentivirus delivering human SNIP1 transgene (System Biosciences CD823A-1) in ultra-low attachment 6-well plates ...
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bioRxiv - Genomics 2020Quote: ... multiple viral supernatants were collected and concentrated using PEG-it Virus Precipitation Solution (System Biosciences LV810A-1). Cells were infected 2-3 times at 12-hour intervals before selection in the appropriate antibiotic.
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bioRxiv - Cell Biology 2020Quote: ... collected after 24 hours and concentrated using Peg-It Virus precipitation solution (cat# LV810A-1, System Biosciences) according to the manufacturer’s manual.
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bioRxiv - Immunology 2024Quote: ... The HEK293TN-hACE2 cell line was generated by lentiviral transduction of HEK293TN (System Bioscience, Cat#LV900A-1) cells as described in Notarbartolo S ...
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bioRxiv - Bioengineering 2023Quote: ... and anti-rabbit HRP-linked secondary antibody (EXOAB-KIT-1) were purchased from System Biosciences (CA, USA). Anti-beta-actin antibody (sc-47778) ...
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bioRxiv - Developmental Biology 2020Quote: ... ESC were washed twice with PBS and incubated with AP Staining kit™ (Cat # AP100R-1, System Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Cas9-MNT1 cells were transfected with Pb459-U6-sgRNA-EF1a-PuroR and piggyBac transposase (PB210PA-1, System Biosciences) in a 1:3 ratio using lipofectamine (Life Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... Culture medium was collected 72 hours post transfection and concentrated with AAVanced Concentration Reagent (#AAV100A-1, System Biosciences).
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bioRxiv - Cell Biology 2020Quote: Lentivirus was generated with PEG-it Virus Precipitation Solution (System Biosciences, Palo Alto, CA, USA no LV810A-1) using a LV H2B-GFP plasmid (Addgene no 25999 ...
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bioRxiv - Immunology 2021Quote: Spike-expressing pseudovirus was generated with System Bioscience’s pPACK-SPIKE packaging system (System Biosciences, Cat # CVD19-50A-1) as per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... BAE-FITC complexes were incubated with 350 μL of Exosome elution buffer (System Biosciences, Inc.; Catalog # CSFLOWBASICA-1) at 25°C for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviruses containing the luciferase expression cassette were produced in 293 TN cells (System Bioscience, catalog number: LV900A-1) by transient transfection with the PSMPUW-Hygro lentiviral vector carrying the luciferase gene and the ViraSafe™ Lentiviral Packaging System (Cell Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... Viral transduction of matured iPS-RPE were aided using TransDux virus transduction reagent (System Biosciences, cat# LV850A-1) and spin infection (800 g ...
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bioRxiv - Cell Biology 2022Quote: ... The pCDH-CMV-MCS-EF1-Blast was generated from pCDH-CMV-MCS-EF1-Hygro (plasmid #CD515B-1; System Biosciences). The annealed synthetic oligonucleotides (Table 2 ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... we developed the excision-only PB transposase plasmid pXPBase-GFP through multiple cloning steps modifying PB220PA-1 (System Biosciences). The ccdB gene was removed to render the plasmid generally propagable ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNAs were then cloned into the peGFP-N1 backbone by Cold Fusion Cloning (System Biosciences, Cat# MC010B-1) following the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2022Quote: ... supernatant containing eluted exosomes were incubated with 1 μL of Exo-FlowIP clearing reagent (System Biosciences, Inc.; Catalog # EXOFLOW32A) at 37°C for 30 min then stored at −80°C.
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bioRxiv - Biochemistry 2023Quote: ... Stable cell lines were generated by cotransfection with the Super PiggyBac Transposase Expression Vector (cat# PB210PA-1, System Biosciences) followed by selection via addition of 5 μg/mL puromycin (cat# ant-pr-1 ...
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bioRxiv - Developmental Biology 2023Quote: ... 2000 ng of the PiggyBac vector plasmid and 500 ng of the PiggyBac transposase plasmid (System Biosciences, PB210PA-1) were mixed in 125 μL Opti-MEM (Gibco ...
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bioRxiv - Genomics 2023Quote: ... The collected virus was concentrated 100-fold after precipitation with PEG-itTM Virus Precipitation Solution (System BioSciences LV810A-1) for 3 days ...
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bioRxiv - Cancer Biology 2023Quote: ... was added to a tube along with purefection reagent and pPackF1 lentiviral packaging kit plasmids (System Biosciences, # LV100A-1) and either PCF1 (empty vector control) ...
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bioRxiv - Bioengineering 2023Quote: Stable HeLa-Fucci cell line was generated using the Super PiggyBac Transposase Expression Vector system (System Biosciences, PB210PA-1) together with tFucci(CA)5 plasmid29 (Addgene #153521) ...
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bioRxiv - Biophysics 2023Quote: ... Cells were transfected with 1500ng of HaloTag-NLS or HaloTag-H2b + 1000ng of piggyBac Transposase (System Biosciences, PB210PA-1) using Lipofectamine2000 (Thermo Fisher Scientific ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... Quantification of successfully integrated piggyBac transposons was performed using a piggyBac qPCR copy number kit (System Biosciences, PBC100A-1) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: Cells were cultured in DMEM/1640 supplemented with 10% exosome-depleted FBS (EXO-FBS-250A-1, SBI System Biosciences, USA) using 10×10 cm2 disks (1×106 cells per disk ...