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Citations for System Biosciences :
1 - 50 of 252 citations for 7 Chloro 3 4 2 hydroxyethyl 1 piperazinyl 1 2 propoxyethyl pyrido 3 4 b pyrazin 2 1H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... 4 mL 5× PEG reagent (System Biosciences, LV825A-1) was added and mixed by pipetting ...
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bioRxiv - Molecular Biology 2020Quote: ... The WPRE 3’-UTR sequence was amplified from the CD813A-1 (System Biosciences) vector.
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bioRxiv - Cancer Biology 2023Quote: ... and incubated overnight at 4°C with 1:1000 primary antibody (CD63 and CD81, rabbit anti-human, EXOAB-CD63A-1/ EXOAB-CD81A-1, System Biosciences, Palo Alto, CA, USA) in TBST ...
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bioRxiv - Molecular Biology 2021Quote: ... for 1 h at room temperature and incubated with primary antibodies overnight at 4 °C with anti-Cd9 ([1:3000; System Biosciences, Palo Alto ...
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bioRxiv - Genetics 2021Quote: ... An in vitro transcribed sgRNA targeting Slc16a11 (5’-AGTCCTAACCTCGCTTGGCT-3’) and hspCas9 mRNA (CAS500A-1, System Biosciences) were microinjected into C57BL/6J mouse zygotes by the Genome Modification Facility at Harvard University ...
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bioRxiv - Microbiology 2022Quote: ... supernatant was diluted (4 to 1 ratio v/v) with PEG-it solution (SBI, System Biosciences), vortexed and incubated continuously rotating overnight at 4°C ...
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bioRxiv - Genomics 2023Quote: ... The lentivirus-containing media was mixed at a 1:4 ratio of PEG-it virus precipitation solution (System Biosciences, Cat. No. LV810A-1) to media ...
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bioRxiv - Microbiology 2020Quote: ... for 2 h at 37°C and plasmid-safe DNase (System Bioscience) overnight at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... the cloning site with Kozak sequence fragment #1 was amplified from the PB-EF1-MCS-IRES-Neo vector (PB533A-2; System Biosciences, Palo Alto, CA, USA) using PCR and the following primers ...
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bioRxiv - Molecular Biology 2021Quote: ... The WPRE (Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element) 3’-UTR sequence was amplified from the CD813A-1 (System Biosciences) vector.
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bioRxiv - Synthetic Biology 2021Quote: ... A total of 4 μg of piggyBac targeting base editor plasmids were transfected with 1 μg of super transposase plasmid (SBI System Biosciences cat# PB210PA-1) using Lipofectamine 3000 following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... mRuby was amplified from PB-EF1α-MCS-IRES-RFP (System Biosciences PB531A-2) by PCR ...
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bioRxiv - Bioengineering 2022Quote: Gene editing of COS-7 cells were performed by electroporation of COS-7 cells with Super PiggyBac plasmid (PB210PA-1, System Biosciences) and one of the 5 plasmids of pBv1-EF-6X ...
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bioRxiv - Immunology 2021Quote: ... 5’ ATACGCGGATCCtcagtagggccgccacac 3’ and cloned into a commercial lentivector containing a GFP reporter gene (pCDH-GFP-Em-CD513B-1 from System Biosciences). To overexpress RUNX2 in human T cells ...
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bioRxiv - Developmental Biology 2020Quote: ... 7 that exhibited distinct spots by transient expression (Supplementary table 1) were subcloned into a piggybac-vector PB510B-1 (System BioSciences) to establish stable lines ...
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bioRxiv - Molecular Biology 2023Quote: ... a minimal piggyBac cargo construct was created by deleting all intervening sequences between the 5’ and 3’ terminal repeats (including core insulators) of the PB-CMV-MCS-EF1α-Puro vector (System Biosciences, PB510B-1). A gBlock (Integrated DNA Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... The corresponding oligos were ordered from Twist BioScience and cloned into an untagged (System BioSciences, PB533A-2), GFP-tagged (pPB_EF1_MCS-EGFP_IRES_Puro ...
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bioRxiv - Cancer Biology 2023Quote: ... Viral particles were harvested 2 days later and concentrated with Peg-itTM (System Biosciences, Palo Alto, CA, USA).
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bioRxiv - Synthetic Biology 2020Quote: ... The human proteins were assigned to 3 Piggybac vectors (System Biosciences, USA) by Golden Gate Assembly method (11 ...
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bioRxiv - Microbiology 2021Quote: ... ARN8 HBEGFKO cells were transfected at 80% confluence in a 6-well plate with pCMV-hHBEGF (2 µg) and PB Transposase vector (0.5 µg) (System Biosciences) using FuGENE® HD (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... Human wild-type or mutant (K4M) histone H3.1 were cloned into pCDH-EF1-MCS-IRES-RFP (System Biosciences, CD531A-2). To express sgRNAs ...
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bioRxiv - Cell Biology 2022Quote: ... one day before the transfection of the expression construct of mCherry-proliferating-cell nuclear antigen (PCNA) or EGFP-ATM based on PB533A-2 (System Biosciences) with a PiggyBac transposon expression vector (PB210PA-1 ...
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bioRxiv - Developmental Biology 2024Quote: ... with the primers 5’-GGGGAATTCGCCACCATGGGTTCTCA-3’ and 5’-CCC GCGGCCGCTCACTTCGCTGTCATCA-3’ was subcloned into EcoRI and NotI sites in the P B-CMV-MCS-EF1α-Puro PiggyBac transposon vector (PB510B-1, System Bioscience).
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bioRxiv - Synthetic Biology 2023Quote: ... and PB-SIN-puro include the backbone and ITR and insulator domains of the piggybac transposon vector PB-EF1α-MCS-IRES-GFP (System Biosciences PB530A-2) and contain a 5’ compound SV40 enhancer with hybrid RSV-MMSV LTR (based on SERS11 design66) ...
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bioRxiv - Immunology 2023Quote: ... Inserts were introduced into the pCDH-CuO-MCS-EF1a-CymR-T2A-Puro SparQ™ All-in-one Cloning and Expression Lentivector (QM800A-1, System Biosciences). The correct sequence of the gene of interest (GOI ...
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bioRxiv - Developmental Biology 2022Quote: ... with 250 μg protein equivalent of AFSC-EVs stained using 2 μL of ExoGlow™-Vivo (Near IR) EV Labeling Kit (System Biosciences, Palo Alto, CA).
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bioRxiv - Cell Biology 2019Quote: ... The purity of exosome fraction was also evaluated with Exosome Array (System Biosciences, EXORAY-4).
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bioRxiv - Bioengineering 2022Quote: ... and Exo-Check™ exosome antibody array (EXORAY200B-4) were purchased from SBI (System Biosciences). miRCURY LNA™ RT kit ...
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bioRxiv - Cell Biology 2020Quote: ... purification of EVs was performed in plasma by UC-I (bellow) and in conditioned medium according to one of the following protocols (Fig.1a):-(Exoquick) ExoQuick® commercial kit (System Biosciences EXOTC10A-1, Palo Alto, CA), following manufacturer’s instructions;
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bioRxiv - Immunology 2021Quote: ... Construct expressing lipin-1-mCherry was amplified by PCR adding 5’Xba-I and Sal-I 3’specific restriction sites and inserted into lentiviral vector pCDH-CMV-MCS-EF1α-copGFP (System Biosciences) after removing an internal Sal-I restriction site by mutagenesis ...
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bioRxiv - Molecular Biology 2021Quote: ... with 1/5 final dilution (System Biosciences, LV810A-1), and the mixture was incubated at 4 °C for 6 hrs ...
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bioRxiv - Developmental Biology 2020Quote: ... around the sgRNA1 target site at +9bp from the start of TBX5 exon 3 was cloned by Cold Fusion (System Biosciences #MC010B) using amplicons from genomic DNA of WTC11 into a construct that was a modification of plasmid pEN114 (Nora et al. ...
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bioRxiv - Cell Biology 2022Quote: ... The NTA measurements rely on light scattering to extract particle size and the number of particles in a sample and the NTA software (Version 2.3) collects data on multiple particles to calculate the hydrodynamic diameter of each particle using the Stokes-Einstein equation (System Biosciences, LLC).
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bioRxiv - Molecular Biology 2019Quote: ... with 1 μg of transposase plasmid (System Biosciences #PB210PA-1) under conditions described above ...
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bioRxiv - Cell Biology 2022Quote: ... a lentivirus expression plasmid (MMIR-1a-1-PA-1 and MMIR-206-PA-1 purchased from System Biosciences) and plasmid mixture for packaging (ViraPower HiPerform Lentiviral Expression Systems ...
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bioRxiv - Biochemistry 2020Quote: ... anti-TSG101 antibody (EXOAB-TSG101-1; System Biosciences, CA; 1:500), anti-CD63 antibody (EXOAB-CD63A-1 ...
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bioRxiv - Biochemistry 2020Quote: ... anti-CD63 antibody (EXOAB-CD63A-1; System Biosciences, CA; 1:500), anti-CD5L antibody (AF2834 ...
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bioRxiv - Neuroscience 2021Quote: ... Lentiviral particles were harvested either by ultracentrifugation at 106,559 g for 2 h at 4°C using a Beckman SW 32 Ti rotor or using the PEG-it Virus Precipitation Solution (System Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Total RNA isolation kits were employed to isolate RNA from cultured iMicroglia (n=4/group; System Bioscience, Palo Alto, CA, USA) and the mouse hippocampal tissues (n=5-6/group ...
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bioRxiv - Cell Biology 2021Quote: ... AP100R-1 (System Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Lentivector-based scrambled miRZip-000 (MZIP000-PA-1) and miRZip-146a (anti-miRNA; MZIP146a-PA-1) in S1505A-1 construct vector were purchased from System Biosciences. Both Scrambled and miRZIP vectors contained copGFP as a fluorescent reporter ...
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bioRxiv - Synthetic Biology 2020Quote: ... Exo-Check Array: A549 protein markers were characterised using a commercially sourced Exo-Check Exosome Antibody Array kit (#EXORAY200A-4, System Biosciences, CA, USA). A549 EVs (100 μg ...
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bioRxiv - Cell Biology 2023Quote: The let-7 overexpression plasmids are commercially available from System Biosciences (SBI). The catalog numbers of the plasmids used in this research are PMIRH000PA-1 (empty vector) ...
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bioRxiv - Cancer Biology 2023Quote: ... along with two housekeeping genes (Supplementary Table 1; CAS9-PR-1 from System Biosciences). The Kcnn4 and Cas9 mRNA levels for each sample were measured in technical duplicates for each primer set ...
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bioRxiv - Developmental Biology 2020Quote: Human SC102A-1 (System Bioscience) and chimpanzee Sandra A iPSC lines (Camp et al. ...
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bioRxiv - Microbiology 2022Quote: HEK293T (System Biosciences, LV900A-1) and HEK293-Spike cells (SARS-CoV-2 Spike (D614)-expressing 293 cells [293-SARS2-S cells ...
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bioRxiv - Developmental Biology 2020Quote: ... DLD-1 cells were co-transfected with a PiggyBac transposase vector (System Biosciences, PB210PA-1) and a PiggyBac-ABA-CRISPRi vector (25 ...
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bioRxiv - Cell Biology 2023Quote: ... 1 million cells were co-transfected with 1 µg ePiggyBac transposase expression plasmid (System Biosciences) and 1 µg each epB-MCP-mScarletI ...
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bioRxiv - Developmental Biology 2023Quote: ... or goat anti-rabbit (1:20,000; EXOAB-KIT-1; System Biosciences, Palo Alto, CA, USA). Protein signals were detected using the SuperSignal West Femto Chemiluminescent kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: An amount of 4 ng of XMIRXpress vector containing Non-Targeting miRNA with Xmotif (cat. no. XMIRXP-NT, System Biosciences LLC, Palo Alto, CA, USA) was introduced into 100 μL Stellar competent E ...