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Citations for System Biosciences :
201 - 245 of 245 citations for Recombinant Human Neuropilin 1 Fc Chimera since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: RNA was isolated from the EVs using the Exoquick RNA column purification kit (EQ808A-1, System Biosciences, Palo Alto, CA, USA). Purified RNA was quantified using a Bioanalyzer and the Agilent RNA 6000 Pico kit (5067-1513 ...
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bioRxiv - Developmental Biology 2020Quote: ... The resulting PB510B-based sgRNA expression vector and PB533-dCas9-EGFP were co-transfected with a transposase expression vector PB200PA-1 (System BioSciences) into MC12 cells ...
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bioRxiv - Microbiology 2021Quote: For cDNA expression of WIPI2B proteins cells were transduced with lentivirus carrying the gene of interest with an N-terminal 3X Ty1 tag (Table S7) on the pCDH-CMV-MCS-T2A-Puro backbone (CD522A-1, System Biosciences). cDNA expression of ATG proteins has been previously described (21 ...
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bioRxiv - Cell Biology 2023Quote: ... The 30 mL of lentiviral supernatant was pooled and concentrated to 1/100th of its original volume (300 µL) in DPBS using PEG-iT (System Biosciences), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: Clones A and E were transfected with a Piggybac-PEmax plasmid with the Piggybac transposase (System Biosciences, Cat. No. PB210PA-1) at a 10:1 molar ratio using the transIT-LT1 transfection reagent (Mirius Bio ...
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bioRxiv - Cancer Biology 2023Quote: ... HA1_BsaI_rev 5’-TAT AGA GAC CGA TGG ATA AAA GCC CAA ATT C-3’ and cloned into the multiple cloning site 1 (MCS1) of the vector MCS1-EF1α-GFP-T2A-Puro-pA-MCS2-PGK-hsvTK (HR700, System Biosciences) using BsrGI (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... U2-OS cells (UC Berkeley BDS Cell Culture Facility) cultured in DMEM (1 g/L glucose, 10% FBS) were transfected with a modified PiggyBac Transposase plasmid (System Biosciences) and a PGK.mNeonGreen-LacI.NLS.IRES.Puro integration plasmid using Fugene6 transfection reagent ...
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bioRxiv - Neuroscience 2024Quote: ... and the medium was changed to the same DMEM but supplemented with 10% exosome-depleted FBS (EXO-FBS-50A-1, System Bioscience). The media was collected on the day of isolation ...
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bioRxiv - Neuroscience 2024Quote: The mouse H3f3a sequence (encoding the histone H3.3A isoform) upstream an IRES and nuclear GFP sequences were cloned into the EcoRI and NotI sites of EEV600A-1 (System Biosciences) to generate the EEV expressing the WT histone H3.3A (supplementary Fig ...
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bioRxiv - Developmental Biology 2024Quote: ... with the primers 5’-GGGGAATTCGCCACCATGGGTTCTCA-3’ and 5’-CCC GCGGCCGCTCACTTCGCTGTCATCA-3’ was subcloned into EcoRI and NotI sites in the P B-CMV-MCS-EF1α-Puro PiggyBac transposon vector (PB510B-1, System Bioscience).
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bioRxiv - Cell Biology 2023Quote: ... designed by CRISPRdirect,101 were cloned into linearized CAG-T7-hspCas9-T2A-RFP-H1-gRNA SmartNuclease vector (CAS721R-1; System Biosciences).
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bioRxiv - Cell Biology 2024Quote: ... PAAKRVKLD) from pCDH-NLSSV40-sfCherry-NLSMyc-Blast (30) were ligated to pCDH-CMV-MCS-EF1-Hygro (plasmid #CD515B-1, System Biosciences) using the Ligation-Convenience Kit (Nippon Gene ...
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bioRxiv - Developmental Biology 2021Quote: ... The amplicon was cloned into a modified version of the self-inactivating lentiviral vector pPS-EF1α-GFP-RFP (System Biosciences, LV603PA-1) where GFP and RFP had been removed and replaced by an hPGK-Puromycin cassette (pSIN-EF1a promoter-BGH polyA-hPGK-Puromycin) ...
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bioRxiv - Cancer Biology 2019Quote: Neuroblastoma cell lines were obtained from the Children’s Hospital of Philadelphia (CHOP) neuroblastoma cell line bank and 293T cells from System Biosciences (LV900A-1); all were cultured as previously described ...
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bioRxiv - Cell Biology 2023Quote: ... The tag donor was also introduced as a minicircle generated with the MC-Easy Minicircle DNA Production Kit according to manufacturer’s instructions (System Biosciences, MN920A-1). Both solutions were combined ...
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bioRxiv - Cell Biology 2023Quote: ... The tag donor was also introduced as a minicircle generated with the MC-Easy Minicircle DNA Production Kit according to manufacturer’s instructions (System Biosciences, MN920A-1). Both solutions were combined ...
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bioRxiv - Immunology 2023Quote: ... Inserts were introduced into the pCDH-CuO-MCS-EF1a-CymR-T2A-Puro SparQ™ All-in-one Cloning and Expression Lentivector (QM800A-1, System Biosciences). The correct sequence of the gene of interest (GOI ...
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bioRxiv - Cell Biology 2024Quote: ... Extracellular vesicles were isolated from 10 mL of cell culture supernatant using the ExoQuick-TC® Exosome Precipitation Solution (cat# EXOTC10A-1; System Biosciences) according to the manufacturer’s instructions with a few modifications ...
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bioRxiv - Molecular Biology 2024Quote: ... The pooled supernatant was then filtered with 0.45 μm membrane and concentrated using the PEG-it virus precipitation solution (System Biosciences #LV810A-1) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Not1 digested and Calf Intestinal Phosphatase treated pCDH-CMV-MCS-EF1α-copGFP Dual Promoter Cloning and Expression Lentivector (System Biosciences LLC, #CD511B-1). Similarly ...
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bioRxiv - Biophysics 2022Quote: ... wildtype U-2 OS cells (ATCC #HTB-96) were transfected with 1000 μg of PiggyBac-HaloTag GR and 250 μg of Super PiggyBac Transposase (System BioSciences #PB200A-1) and 3 μL of TransIt LT1 (Mirus #MIR2305) ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ACE2 genes were cloned into pCDH-EF1α-MCS-BGH-PGK-GFP-T2A-Puro Cloning and Expression Lentivector (System Biosciences, catalogue number CD550A-1), where the EF1α promoter was replaced with the full length EF1α using NheI and ClaI restriction enzymes (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Each of the individual rTetR-effector plasmids was randomly integrated into this reporter line by co-transfecting 250 ng Super PiggyBac Transposase expression vector (System Biosciences #PB200PA-1) and 750 ng of rTetR-effector donor vector ...
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bioRxiv - Synthetic Biology 2020Quote: The plasmids containing the rTetR-effector fusions were cloned into the PB-CMV-MCS-EF1α-Puro PiggyBac vector backbone (System Biosciences #PB510B-1), which was further modified via Gibson Assembly with the following components ...
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bioRxiv - Molecular Biology 2023Quote: ... a minimal piggyBac cargo construct was created by deleting all intervening sequences between the 5’ and 3’ terminal repeats (including core insulators) of the PB-CMV-MCS-EF1α-Puro vector (System Biosciences, PB510B-1). A gBlock (Integrated DNA Technologies ...
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bioRxiv - Physiology 2023Quote: Isolated Evs were processed for total RNA isolation using the SeraMir Exosome RNA purification kit (Cat #RA808A-1, System Biosciences, Palo Alto, CA), according to the manufacturer’s instructions provided with the kit ...
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bioRxiv - Genomics 2023Quote: ... Fragments were resuspended at a final concentration of 100ng/ul in EB buffer and then cloned into a PB-CMV-MCS-EF1α-Puro PiggyBac cDNA Cloning and Expression Vector (PB vector hereafter, System Biosciences #PB510B-1). Following ligation ...
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bioRxiv - Immunology 2023Quote: ... or luciferase used the same packaging and envelope plasmids as described previously with the respective 3rd generation plasmids: pCDH-MSCV-MCS-EF1-GFP-T2A-Puro (System Biosciences, CD713B-1), pLenti-mCherry (derived from pLenti-III-EF1a ...
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bioRxiv - Cancer Biology 2021Quote: ... Media containing virus was collected both 48 and 72 hours post-transfection and viral particles were precipitated using PEG-it™ Virus Precipitation Solution (System Biosciences, LV810A-1). To transduce cells with virus cells were treated with both virus (MOI 0.5-1.0 ...
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bioRxiv - Cell Biology 2022Quote: Bare 293T cells were first co-transfected using PEI47 with the H2B-mTagBFP2 vector and Super PiggyBac Transposase-expressing vector (System Biosciences Inc. # PB210PA-1) via polyethylenimine (Sigma #408727-100mL ...
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bioRxiv - Developmental Biology 2022Quote: ... The amplified coding sequences followed by p2a-tdTomato were cloned downstream of the endothelial specific fli1a promotor into a Tol2 enabled vector using the Cold Fusion Cloning kit (System Biosciences, Cat# MC010B-1).
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bioRxiv - Cancer Biology 2022Quote: ... Media was collected at 48 and 72 hours post-transfection and viral particles were precipitated using PEG-it™ Solution (System Biosciences, LV810A-1). To transduce ...
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bioRxiv - Molecular Biology 2022Quote: ... pT-mAppleT2Apuro was generated by PCR amplifying the T2A peptide and puromycin resistance gene from PB-CMV-MCS-GreenPuro (System Biosciences, Cat# PB513B-1) and cloning into pT-mApple (Vectorbuilder) ...
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bioRxiv - Cell Biology 2019Quote: ... the collected exosomes were further purified with anti-CD9 antibody-coated magnetic beads (CD9 Exo-Flow Exosome Purification Kit, System Biosciences, Cat. EXOTC10A-1) as in (Rim and Kim ...
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bioRxiv - Synthetic Biology 2022Quote: ... Plasmids encoding different genetic payloads (transfer plasmids) were designed in SnapGene software (GSL Biotech LLC) and sub-cloned into lentivirus vector plasmid (System Biosciences, Cat #CD510B-1) or PiggyBac Transposon vector plasmid (System Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... 0N4R tau was replaced with 2N4R tau after inserting 2N4R tau between BamHI and SalI restriction sites using a cold fusion cloning kit (System Biosciences, Catalog # MC100B-1). A Q5 Site-Directed Mutagenesis Kit (New England BioLabs ...
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bioRxiv - Biochemistry 2021Quote: MDA-MB-231 or HEK 293T cells were grown to ~80% confluency in 14 × 150 mm dishes with 10% exosome-depleted FBS (Exo-FBS from System Biosciences, EXO-FBS-250A-1) in DMEM GlutaMAX media ...
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bioRxiv - Cancer Biology 2020Quote: ... UCHL1 knockdown was also carried out by transducing HGSOC cells with control or UCHL1 shRNA lentiviral virus particles (Santacruz Biotechnology; cat# sc-108080 and sc-42304-V) using TransDux™ MAX (System Biosciences; cat#LV860A-1). Cells were treated with Carfilzomib (cat# S2853 ...
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bioRxiv - Cell Biology 2021Quote: ... the cloning site with Kozak sequence fragment #1 was amplified from the PB-EF1-MCS-IRES-Neo vector (PB533A-2; System Biosciences, Palo Alto, CA, USA) using PCR and the following primers ...
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bioRxiv - Microbiology 2020Quote: ... HEK293T cells (ATCC® ACS-4500™) were transiently transfected with hACE2 fusion BFP encoding PB510 plasmid using PiggyBac Transposon System (System Biosciences, PB210PA-1), followed by addition of 2 μg/mL puromycin 6 h post-transfection ...
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bioRxiv - Bioengineering 2022Quote: ... EVs were also quantified based on the amount of total immunoreactive CD63 within isolated EV samples using the ExoELISA-ULTRA Complete Kit (System Biosciences, EXEL-ULTRA-CD63-1) per the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: 16 million K562 cells (8 million K562-VP64 cells and 8 million K562-VPR cells) were transfected with the gRNA library and the piggyBac transposase (System Biosciences, Cat. No. PB210PA-1) at a 20:1 molar ratio of library:transposase using a Lonza 4D nucleofector and the Lonza nucleofector protocol for K562 cells ...
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bioRxiv - Cell Biology 2024Quote: ... The EPAC1-V5-APEX2 fragment was linearized with EcoRI and NotI and cloned into the pCDH-CMV-MCS-EF1α-Puro (System Biosciences, Catalog no. CD510B-1) lentiviral vector ...
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bioRxiv - Cell Biology 2020Quote: ... purification of EVs was performed in plasma by UC-I (bellow) and in conditioned medium according to one of the following protocols (Fig.1a):-(Exoquick) ExoQuick® commercial kit (System Biosciences EXOTC10A-1, Palo Alto, CA), following manufacturer’s instructions;
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bioRxiv - Molecular Biology 2022Quote: ... The siRNAs for CLC3 and CLC7 were constructed using double-promoter pFIV-H1/U6 siRNA cloning and expression vectors (System Biosciences, Palo Alto, CA, USA; Cat: SI111A-1), according to the manufacturer’s instructions ...