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Citations for System Biosciences :
201 - 250 of 318 citations for Dengue Virus Serotype 1 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: The transfection of FAPs with pCT-CD63-GFP plasmid (#CYTO120-PA-1, System Biosciences) and with a GFP-control vector (mock ...
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bioRxiv - Molecular Biology 2022Quote: ... EVs were isolated using ExoQuick-TC (EXOTC50A-1, System Biosciences, Palo Alto, CA, USA) according to manufacturer instructions ...
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bioRxiv - Genetics 2019Quote: ... We transfected the plasmids with Super PiggyBac Transposase Expression Vector (System Biosciences, PB210PA-1) using Lipofectamine™ 3000 Transfection Reagent ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and ligated into the CAGs-MCS-Enhanced Episomal Vector (EEV) (System Biosciences, EEV600A-1) linearized with AgeI and KpnI ...
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bioRxiv - Neuroscience 2022Quote: ... and microglial enrichment was conducted per manufacturer’s instructions (System Biosciences, Inc.; Catalog # CSFLOWBASICA-1). Briefly ...
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bioRxiv - Immunology 2023Quote: ... pCDHv6_PTPRZ1 (derived from pCDH-MSCV-MCS-EF1-GFP-T2A-Puro, System Biosciences, CD713B-1), with packaging plasmids pMDLg/pRRE (RRID:Addgene_12251) ...
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bioRxiv - Developmental Biology 2024Quote: ... The expression vector coding GCaMP6s was introduced into MDCK cells with Super PiggyBac transposase (System Bioscience). After a few days ...
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bioRxiv - Bioengineering 2021Quote: ... RFP-expressing GBM12 (GBM12-RFP) was generated by transduction with lentivirus (System Biosciences, LL110VA-1) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2020Quote: ... EV depleted FCS was either commercially obtained (System Biosciences Catalog no. EXO-FBS-250A-1) or prepared by ultracentrifugation of the FCS used at 120,000 x g for 5 hours ...
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bioRxiv - Microbiology 2020Quote: ... Fusion was performed with the Cold Fusion Cloning Kit (SBI System Biosciences, Cat. #MC010B-1).
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bioRxiv - Cell Biology 2021Quote: Inducible Cas9 iPSCs were generated using an AAVS1 TALE-Nuclease KIT (GE60xA-1; System Biosciences) and an iCas9 HR donor plasmid containing a TRE3G promoter-driven spCas9 with a C-terminal MYC NLS followed by a CMV promoter-driven Tet-On-3G and a neomycin resistance adapted from Ihry et al ...
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bioRxiv - Cell Biology 2023Quote: ... from 3T3-L1 genomic DNA and cloned into the HR110-PA-1 vector (System Biosciences). Primer sequences to amplify the 5′- and 3′-side genomic fragments for homologous recombination are as follows ...
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bioRxiv - Cancer Biology 2023Quote: 2.5×106 IFU/ml of pooled lenti-miRNA lentivirus library (System Biosciences Cat. #PMIRHPLVHT-1) was mixed with 4 mg/mL polybrene (Sigma ...
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bioRxiv - Physiology 2023Quote: ... and 5 µL of de-fibrination reagent (Cat #TMEXO-1, System Biosciences, Palo Alto, CA) was added to the plasma to remove clotting factors prior to the isolation of EVs ...
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bioRxiv - Immunology 2024Quote: ... The PCR fragments were cloned into a PiggyBac mammalian expression vector (System Biosciences, PB210PA-1), modified to harbor a Secrecon-AA signal peptide111 for secretion at the N-terminus ...
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bioRxiv - Immunology 2019Quote: ... and used to transduce pre-activated primary human CD4+ T cells in combination with Transdux (System Biosciences). GFP+ cells were FACS sorted 48h later ...
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bioRxiv - Cell Biology 2021Quote: ... The vectors were transduced into HeLa cells using the PiggyBac transposon system (System Biosciences, Palo Alto, CA), and the cells were selected for 2 weeks with 2 μg/ml of puromycin ...
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bioRxiv - Immunology 2020Quote: ... All other 293T-GFP responder cell lines were constructed by lentiviral transduction (pCDH vector backbone, System Biosciences). All transient expression plasmids were cloned into the pCS2+ vector using standard restriction cloning ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lines were immediately cryopreserved and maintained in selection medium containing 60 μg/mL cumate (System Biosciences) for at least one passage prior to assay.
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bioRxiv - Systems Biology 2020Quote: ... GFP gene was amplified from pGreenPuro shRNA Cloning and Expression Lentivector (CMV; System biosciences, SI505A-1) and sub-cloned into ITR-CAG-DEST-IRES-Neomycin-ITR plasmid (generous gift from Marc Supprian Schmidt) ...
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bioRxiv - Neuroscience 2021Quote: ... The viral particles were precipitated from the media using AAVanced concentration reagent (System Biosciences #AAV100A-1) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... in DMEM medium supplemented with 10% FBS depleted of exosomes (System Biosciences, EXO-FBS-250A-1). Cells were maintained in this medium for 24 h before supernatant collection for EV purification ...
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bioRxiv - Cell Biology 2022Quote: ... These constructs are based on an empty parent minicircle cloning vector (MN100A-1; System Biosciences (SBI), Palo Alto ...
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bioRxiv - Neuroscience 2022Quote: ... supernatants were incubated with 120 μL ExoQuick Exosome Precipitation solution (System Biosciences, Inc.; Catalog # EXOQ5TM-1) for 30 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... supernatant was diluted (4 to 1 ratio v/v) with PEG-it solution (SBI, System Biosciences), vortexed and incubated continuously rotating overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... The CRISPR gRNA (5pg per embryo) and Cas9 mRNA (150pg per embryo, System Biosciences CAS500A-1) were injected into single-cell embryos ...
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bioRxiv - Molecular Biology 2023Quote: ... the SpyCas9-ABE-tracrRNA PiggyBac transposon plasmid and PiggyBac transposase expression plasmid (System Biosciences PB210PA-1) were mixed in a 2:1 molar ratio and transfected into the HEK293T-dGFP reporter cell line using Lipofectamine 3000 (ThermoFisher L3000015) ...
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bioRxiv - Cancer Biology 2023Quote: EVs from biological fluids were isolated using ExoQuick exosome precipitation solution (System Biosciences, SBI-EXOQ5A-1). Plasma and serum samples were centrifuged at 3,000 × g for 15 min to remove cells and cell debris ...
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bioRxiv - Cancer Biology 2023Quote: ... A second ESR1-DAB2 plasmid in a pCDH-CMV-MCS-ER1α-Puro (System Biosciences #CD510B-1) was received as a kind gift from Dr ...
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bioRxiv - Molecular Biology 2024Quote: ... Lentiviruses were generated by transfecting the plasmids using pPACKH1 Packaging Plasmid Mix (LV500A-1; System Biosciences) into a 293T cell line (LV900A-1 ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... We created the PB transposase plasmid pSPBase by removing the ccdB gene from PB210PA-1 (System Biosciences) to render it generally propagable ...
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bioRxiv - Genetics 2021Quote: ... An in vitro transcribed sgRNA targeting Slc16a11 (5’-AGTCCTAACCTCGCTTGGCT-3’) and hspCas9 mRNA (CAS500A-1, System Biosciences) were microinjected into C57BL/6J mouse zygotes by the Genome Modification Facility at Harvard University ...
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bioRxiv - Microbiology 2020Quote: ... cDNA sequences were cloned into the PB-CMV-MCS-EF1α-Puro vector (PB510B-1, SBI System Biosciences) where the CMV promoter was replaced by a PGK promoter ...
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bioRxiv - Microbiology 2021Quote: ... the gfp-lem8 fusion was inserted into pCDH-CMV-MCS-EF1a-Puro (System Biosciences, cat# CD510B-1). The plasmids were co-transfected with pMD2.G (gift from Dr ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: We used the human iPSC lines mciPS (model no. SC301A-1; System Biosciences, Palo Alto, CA, USA), SBAD2 [75] ...
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bioRxiv - Bioengineering 2023Quote: ... and anti-rabbit HRP-linked secondary antibody (EXOAB-KIT-1) were purchased from System Biosciences (CA, USA). Anti-beta-actin antibody (sc-47778) ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmids for stable cell line construction were generated by Gibson cloning into pCDH-EF1-CymR-T2A-Neo (System Bioscience). Sequences of all plasmids were confirmed by Sanger sequencing.
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bioRxiv - Molecular Biology 2020Quote: The Jurkat cell transductions were done with multiple viral titers using TransDux™ MAX Lentivirus Transduction Reagent (System Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmids for stable cell line construction were generated by Gibson cloning into pCDH-EF1-CymR-T2A-Neo (System Bioscience) vector ...
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bioRxiv - Developmental Biology 2020Quote: ... ESC were washed twice with PBS and incubated with AP Staining kit™ (Cat # AP100R-1, System Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Culture medium was collected 72 hours post transfection and concentrated with AAVanced Concentration Reagent (#AAV100A-1, System Biosciences).
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bioRxiv - Immunology 2021Quote: Spike-expressing pseudovirus was generated with System Bioscience’s pPACK-SPIKE packaging system (System Biosciences, Cat # CVD19-50A-1) as per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... BAE-FITC complexes were incubated with 350 μL of Exosome elution buffer (System Biosciences, Inc.; Catalog # CSFLOWBASICA-1) at 25°C for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... 500 μL of human plasma were incubated with 5 μL purified thrombin (System Biosciences, Inc.; Catalog # TMEXO-1) at room temperature for 5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... stable HEK293-F cell lines for each cytokine were prepared through cloning into the expression cassette of PiggyBac (System Biosciences) transposon vector ...
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bioRxiv - Molecular Biology 2021Quote: The primary human T cell transductions were done with multiple viral titers using TransDux™ MAX Lentivirus Transduction Reagent (System Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... with the respective lamin containing or empty (Mock) vectors into HEK 293-TN cells using PureFection nanotechnology-based transfection reagent (System Biosciences). The medium was exchanged the next day and replaced by antibiotics-free DMEM containing 10% FCS ...
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bioRxiv - Immunology 2020Quote: ... NK92 CD56-KO cells were transduced with pBabe-puro-NCAM mApple viral particles in the presence of MAX Enhancer and TransDux (System Biosciences). Within 48 hours cells were sorted for CD56 and mApple and cells were expanded and maintained under antibiotic selection with regular phenotypic validation.
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bioRxiv - Microbiology 2020Quote: The 3’UTR reporter plasmids were co-transfected into HEK293T cells using PEI max together with the indicated pCDH-EF1-MCS expressing plasmids (System Biosciences) containing a cloned pre-miRNA gene of interest ...
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bioRxiv - Immunology 2022Quote: ... encoding HLA-A*02:01 or HLA-A*03:01 by spinoculation with ultracentrifuge concentrated viral particles produced from 293T-HEK-TN cells (System biosciences), followed by sorting of cells stained with anti-human HLA-A ...