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Citations for System Biosciences :
201 - 250 of 258 citations for 6H Pyrazolo 3 4 b pyridin 6 one 3 cyclobutyl 1 2 4 5 tetrahydro 4 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... and also cloned into pCDH-MSCV-MCS-EF1α-GFP+Puro lentiviral vector (System Biosciences, Cat# CD713B-1, Palo Alto, CA) for constitutive expression ...
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bioRxiv - Genetics 2019Quote: To produce lentiviral cell lines containing pGreenFire1™ Pathway Reporter lentivector (Cat#TR010PA-N and Cat#TR000PA-1, System Bioscience) expressing the MUC5B promoter region and controls ...
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bioRxiv - Neuroscience 2022Quote: ... The lentivirus was concentrated with the use of the PEG-it virus precipitation solution (2.5 ml, System Biosciences, LV810A-1) for 2-3 days at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The N-term Flag-HA-MED4 cDNA was sub-cloned in pCDH-EF1-MCS-IRES-Neo (System Biosciences; CD502A-1) and verified by sequencing ...
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bioRxiv - Genetics 2023Quote: The PB EGFP donor vector (PB-CMV-MCS-EF1ɑ-GFP, #PB511B-1) was purchased from System Biosciences (Palo Alto, CA). The PiggyBac transposase (PBase ...
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bioRxiv - Molecular Biology 2021Quote: ... EVs were isolated from tissue culture media using a commercial kit containing a polyethylene glycol (PEG) solution (ExoQuick-TC ULTRA kit, EQULTRA-20TC-1; System Biosciences) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2020Quote: ... overnight at 37°C and cloned into a linearized pCDH-EF1alpha-MCS-BGH-PGK-GFP-T2A-Puro lentivector (CD550A-1, System Biosciences) using T4 DNA ligase (New England BioLabs ...
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bioRxiv - Cancer Biology 2020Quote: The piggyBac transposon vector was constructed by inserting a random 10-bp DNA barcode sequence between the CMV promoter and the multiple cloning site of the piggyBac dual promoter vector (PB513B-1; System Biosciences). The GFP sequence in the piggyBac vector was deleted by a site-directed mutagenesis technique for the direct repeat–green fluorescent protein (DR-GFP ...
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bioRxiv - Biophysics 2021Quote: ... so that high expression of the binder could be achieved only after addition of the cumate inducer (QM100A-1, System Biosciences). (ArrayG/N)16x has a total size of 255.6 kDa and of 689.2 kDa when fully occupied with mwGFP ...
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bioRxiv - Cell Biology 2021Quote: TYR-G1-G4 knockout cell lines were transfected with the Pb-TRE3G-TYR-HygR plasmid and piggyBac transposase (PB210PA-1, System Biosciences) in a 1:3 ratio using lipofectamine (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... the piggyBac transposon plasmid that contains the desired transgene and a piggyBac transposase plasmid (PB210PA-1, System Biosciences, Palo Alto, CA) were electroporated into TP53-/- RPE-1 cells simultaneously using the Neon electroporation system according to the manufacturer’s instruction (Invitrogen) ...
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bioRxiv - Bioengineering 2022Quote: Gene editing of COS-7 cells were performed by electroporation of COS-7 cells with Super PiggyBac plasmid (PB210PA-1, System Biosciences) and one of the 5 plasmids of pBv1-EF-6X ...
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bioRxiv - Neuroscience 2022Quote: ... HEK 293T cells cultured in 10-cm Petri dishes at a 50-60% confluence were co-transfected with 10 μg lentiviral plasmid expressing miR-124-1 precursor under the CMV promoter and GFP under the EF1 promoter (SBI System Biosciences) or 10 μg lentiviral plasmid expressing GFP under the CMV promoter and the packaging plasmids pVSV-G (3.5 μg) ...
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bioRxiv - Neuroscience 2022Quote: ... The open reading frame from of murine XPR1 was tagged with a sequence for the HA-epitope and cloned into PiggyBac expression vector PB510B-1 (System Biosciences) with XbaI and NotI ...
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bioRxiv - Molecular Biology 2022Quote: RNA was isolated from the EVs using the Exoquick RNA column purification kit (EQ808A-1, System Biosciences, Palo Alto, CA, USA). Purified RNA was quantified using a Bioanalyzer and the Agilent RNA 6000 Pico kit (5067-1513 ...
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bioRxiv - Developmental Biology 2020Quote: ... The resulting PB510B-based sgRNA expression vector and PB533-dCas9-EGFP were co-transfected with a transposase expression vector PB200PA-1 (System BioSciences) into MC12 cells ...
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bioRxiv - Microbiology 2021Quote: For cDNA expression of WIPI2B proteins cells were transduced with lentivirus carrying the gene of interest with an N-terminal 3X Ty1 tag (Table S7) on the pCDH-CMV-MCS-T2A-Puro backbone (CD522A-1, System Biosciences). cDNA expression of ATG proteins has been previously described (21 ...
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bioRxiv - Cell Biology 2023Quote: ... The 30 mL of lentiviral supernatant was pooled and concentrated to 1/100th of its original volume (300 µL) in DPBS using PEG-iT (System Biosciences), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: Clones A and E were transfected with a Piggybac-PEmax plasmid with the Piggybac transposase (System Biosciences, Cat. No. PB210PA-1) at a 10:1 molar ratio using the transIT-LT1 transfection reagent (Mirius Bio ...
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bioRxiv - Cancer Biology 2023Quote: ... HA1_BsaI_rev 5’-TAT AGA GAC CGA TGG ATA AAA GCC CAA ATT C-3’ and cloned into the multiple cloning site 1 (MCS1) of the vector MCS1-EF1α-GFP-T2A-Puro-pA-MCS2-PGK-hsvTK (HR700, System Biosciences) using BsrGI (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... U2-OS cells (UC Berkeley BDS Cell Culture Facility) cultured in DMEM (1 g/L glucose, 10% FBS) were transfected with a modified PiggyBac Transposase plasmid (System Biosciences) and a PGK.mNeonGreen-LacI.NLS.IRES.Puro integration plasmid using Fugene6 transfection reagent ...
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bioRxiv - Cell Biology 2023Quote: ... designed by CRISPRdirect,101 were cloned into linearized CAG-T7-hspCas9-T2A-RFP-H1-gRNA SmartNuclease vector (CAS721R-1; System Biosciences).
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bioRxiv - Neuroscience 2024Quote: The mouse H3f3a sequence (encoding the histone H3.3A isoform) upstream an IRES and nuclear GFP sequences were cloned into the EcoRI and NotI sites of EEV600A-1 (System Biosciences) to generate the EEV expressing the WT histone H3.3A (supplementary Fig ...
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bioRxiv - Neuroscience 2024Quote: ... and the medium was changed to the same DMEM but supplemented with 10% exosome-depleted FBS (EXO-FBS-50A-1, System Bioscience). The media was collected on the day of isolation ...
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bioRxiv - Cell Biology 2024Quote: ... PAAKRVKLD) from pCDH-NLSSV40-sfCherry-NLSMyc-Blast (30) were ligated to pCDH-CMV-MCS-EF1-Hygro (plasmid #CD515B-1, System Biosciences) using the Ligation-Convenience Kit (Nippon Gene ...
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bioRxiv - Developmental Biology 2021Quote: ... The amplicon was cloned into a modified version of the self-inactivating lentiviral vector pPS-EF1α-GFP-RFP (System Biosciences, LV603PA-1) where GFP and RFP had been removed and replaced by an hPGK-Puromycin cassette (pSIN-EF1a promoter-BGH polyA-hPGK-Puromycin) ...
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bioRxiv - Molecular Biology 2020Quote: MDEs were isolated from G1-G3 bovine and human milk samples (1.5 mL/sample) using the EQ reagent (System Biosciences: EXOQ5A-1) (13,14,38,40,44) ...
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bioRxiv - Cancer Biology 2019Quote: Neuroblastoma cell lines were obtained from the Children’s Hospital of Philadelphia (CHOP) neuroblastoma cell line bank and 293T cells from System Biosciences (LV900A-1); all were cultured as previously described ...
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bioRxiv - Molecular Biology 2019Quote: HeLa cervix carcinoma cells (American Tissue Culture Collection: Manassas, VA, USA) and human embryonic kidney (HEK) 293TN cells (System Biosciences, LV900A-1) were cultured using Dulbecco’ s Modified Eagle Medium (DMEM ...
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bioRxiv - Cell Biology 2023Quote: ... The tag donor was also introduced as a minicircle generated with the MC-Easy Minicircle DNA Production Kit according to manufacturer’s instructions (System Biosciences, MN920A-1). Both solutions were combined ...
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bioRxiv - Cell Biology 2023Quote: ... The tag donor was also introduced as a minicircle generated with the MC-Easy Minicircle DNA Production Kit according to manufacturer’s instructions (System Biosciences, MN920A-1). Both solutions were combined ...
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bioRxiv - Molecular Biology 2024Quote: ... The pooled supernatant was then filtered with 0.45 μm membrane and concentrated using the PEG-it virus precipitation solution (System Biosciences #LV810A-1) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Extracellular vesicles were isolated from 10 mL of cell culture supernatant using the ExoQuick-TC® Exosome Precipitation Solution (cat# EXOTC10A-1; System Biosciences) according to the manufacturer’s instructions with a few modifications ...
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bioRxiv - Cell Biology 2021Quote: ... Not1 digested and Calf Intestinal Phosphatase treated pCDH-CMV-MCS-EF1α-copGFP Dual Promoter Cloning and Expression Lentivector (System Biosciences LLC, #CD511B-1). Similarly ...
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bioRxiv - Biophysics 2022Quote: ... wildtype U-2 OS cells (ATCC #HTB-96) were transfected with 1000 μg of PiggyBac-HaloTag GR and 250 μg of Super PiggyBac Transposase (System BioSciences #PB200A-1) and 3 μL of TransIt LT1 (Mirus #MIR2305) ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ACE2 genes were cloned into pCDH-EF1α-MCS-BGH-PGK-GFP-T2A-Puro Cloning and Expression Lentivector (System Biosciences, catalogue number CD550A-1), where the EF1α promoter was replaced with the full length EF1α using NheI and ClaI restriction enzymes (New England Biolabs) ...
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bioRxiv - Cancer Biology 2021Quote: ... was constructed by inserting human HAUSP-WT or HAUSP-CS ORF cDNA into the pCDH-CMV-MCS-EF1a-Puro vector (System Biosciences, CD510B-1). The lentiviral plasmid expressing SOX2 was constructed by cloning human SOX2 ORF cDNA into the pCDH-CMV-MCS-EF1a-Puro vector (System Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... The lentiviral plasmid expressing SOX2 was constructed by cloning human SOX2 ORF cDNA into the pCDH-CMV-MCS-EF1a-Puro vector (System Biosciences, CD510B-1) or PCW57.1 vector (Addgene ...
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bioRxiv - Synthetic Biology 2020Quote: ... Each of the individual rTetR-effector plasmids was randomly integrated into this reporter line by co-transfecting 250 ng Super PiggyBac Transposase expression vector (System Biosciences #PB200PA-1) and 750 ng of rTetR-effector donor vector ...
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bioRxiv - Synthetic Biology 2020Quote: The plasmids containing the rTetR-effector fusions were cloned into the PB-CMV-MCS-EF1α-Puro PiggyBac vector backbone (System Biosciences #PB510B-1), which was further modified via Gibson Assembly with the following components ...
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bioRxiv - Physiology 2023Quote: Isolated Evs were processed for total RNA isolation using the SeraMir Exosome RNA purification kit (Cat #RA808A-1, System Biosciences, Palo Alto, CA), according to the manufacturer’s instructions provided with the kit ...
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bioRxiv - Genomics 2023Quote: ... Fragments were resuspended at a final concentration of 100ng/ul in EB buffer and then cloned into a PB-CMV-MCS-EF1α-Puro PiggyBac cDNA Cloning and Expression Vector (PB vector hereafter, System Biosciences #PB510B-1). Following ligation ...
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bioRxiv - Immunology 2023Quote: ... or luciferase used the same packaging and envelope plasmids as described previously with the respective 3rd generation plasmids: pCDH-MSCV-MCS-EF1-GFP-T2A-Puro (System Biosciences, CD713B-1), pLenti-mCherry (derived from pLenti-III-EF1a ...
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bioRxiv - Cancer Biology 2021Quote: ... Media containing virus was collected both 48 and 72 hours post-transfection and viral particles were precipitated using PEG-it™ Virus Precipitation Solution (System Biosciences, LV810A-1). To transduce cells with virus cells were treated with both virus (MOI 0.5-1.0 ...
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bioRxiv - Cell Biology 2022Quote: Bare 293T cells were first co-transfected using PEI47 with the H2B-mTagBFP2 vector and Super PiggyBac Transposase-expressing vector (System Biosciences Inc. # PB210PA-1) via polyethylenimine (Sigma #408727-100mL ...
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bioRxiv - Developmental Biology 2022Quote: ... The amplified coding sequences followed by p2a-tdTomato were cloned downstream of the endothelial specific fli1a promotor into a Tol2 enabled vector using the Cold Fusion Cloning kit (System Biosciences, Cat# MC010B-1).
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bioRxiv - Cancer Biology 2022Quote: ... Media was collected at 48 and 72 hours post-transfection and viral particles were precipitated using PEG-it™ Solution (System Biosciences, LV810A-1). To transduce ...
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bioRxiv - Molecular Biology 2022Quote: ... pT-mAppleT2Apuro was generated by PCR amplifying the T2A peptide and puromycin resistance gene from PB-CMV-MCS-GreenPuro (System Biosciences, Cat# PB513B-1) and cloning into pT-mApple (Vectorbuilder) ...
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bioRxiv - Cell Biology 2019Quote: ... the collected exosomes were further purified with anti-CD9 antibody-coated magnetic beads (CD9 Exo-Flow Exosome Purification Kit, System Biosciences, Cat. EXOTC10A-1) as in (Rim and Kim ...
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bioRxiv - Synthetic Biology 2022Quote: ... Plasmids encoding different genetic payloads (transfer plasmids) were designed in SnapGene software (GSL Biotech LLC) and sub-cloned into lentivirus vector plasmid (System Biosciences, Cat #CD510B-1) or PiggyBac Transposon vector plasmid (System Biosciences ...