Labshake search
Citations for System Biosciences :
151 - 200 of 273 citations for Corticosterone ELISA Kit 1 Whole Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... Ceg3 was inserted into pCDH-CMV-MCS-EF1a-RFP (System Biosciences cat# CD512B-1), which was transfected together with pMD2.G (Addgene plasmid #12259 ...
-
bioRxiv - Cell Biology 2020Quote: The transfection of FAPs with pCT-CD63-GFP plasmid (#CYTO120-PA-1, System Biosciences) and with a GFP-control vector (mock ...
-
bioRxiv - Molecular Biology 2022Quote: ... EVs were isolated using ExoQuick-TC (EXOTC50A-1, System Biosciences, Palo Alto, CA, USA) according to manufacturer instructions ...
-
bioRxiv - Genetics 2019Quote: ... We transfected the plasmids with Super PiggyBac Transposase Expression Vector (System Biosciences, PB210PA-1) using Lipofectamine™ 3000 Transfection Reagent ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... and ligated into the CAGs-MCS-Enhanced Episomal Vector (EEV) (System Biosciences, EEV600A-1) linearized with AgeI and KpnI ...
-
bioRxiv - Neuroscience 2022Quote: ... and microglial enrichment was conducted per manufacturer’s instructions (System Biosciences, Inc.; Catalog # CSFLOWBASICA-1). Briefly ...
-
bioRxiv - Cell Biology 2023Quote: ... and HEK 293TN (LV900A-1) cells were purchased from System Biosciences (Palo Alto, USA). Mouse mesothelial cells (MCs ...
-
bioRxiv - Genetics 2023Quote: ... cells were transfected with 0.72 μg of piggyBac transposase plasmid (System Biosciences PB210PA-1) and 1.78 μg of pCX4.1 plasmid using Lipofectamine 3000 transfection reagent ...
-
bioRxiv - Immunology 2023Quote: ... pCDHv6_PTPRZ1 (derived from pCDH-MSCV-MCS-EF1-GFP-T2A-Puro, System Biosciences, CD713B-1), with packaging plasmids pMDLg/pRRE (RRID:Addgene_12251) ...
-
bioRxiv - Neuroscience 2024Quote: Total RNA isolation kits were employed to isolate RNA from cultured iMicroglia (n=4/group; System Bioscience, Palo Alto, CA, USA) and the mouse hippocampal tissues (n=5-6/group ...
-
bioRxiv - Bioengineering 2021Quote: ... RFP-expressing GBM12 (GBM12-RFP) was generated by transduction with lentivirus (System Biosciences, LL110VA-1) according to the manufacturer’s instruction ...
-
bioRxiv - Cell Biology 2020Quote: ... EV depleted FCS was either commercially obtained (System Biosciences Catalog no. EXO-FBS-250A-1) or prepared by ultracentrifugation of the FCS used at 120,000 x g for 5 hours ...
-
bioRxiv - Cell Biology 2023Quote: ... from 3T3-L1 genomic DNA and cloned into the HR110-PA-1 vector (System Biosciences). Primer sequences to amplify the 5′- and 3′-side genomic fragments for homologous recombination are as follows ...
-
bioRxiv - Cancer Biology 2023Quote: 2.5×106 IFU/ml of pooled lenti-miRNA lentivirus library (System Biosciences Cat. #PMIRHPLVHT-1) was mixed with 4 mg/mL polybrene (Sigma ...
-
bioRxiv - Physiology 2023Quote: ... and 5 µL of de-fibrination reagent (Cat #TMEXO-1, System Biosciences, Palo Alto, CA) was added to the plasma to remove clotting factors prior to the isolation of EVs ...
-
bioRxiv - Immunology 2024Quote: ... The PCR fragments were cloned into a PiggyBac mammalian expression vector (System Biosciences, PB210PA-1), modified to harbor a Secrecon-AA signal peptide111 for secretion at the N-terminus ...
-
bioRxiv - Molecular Biology 2024Quote: ... Purified viral particles were transduced into MIN6 cells using TransDux MAX (LV860A-1; System Biosciences) and cultured for 48-72 hours in complete DMEM (11965-092 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Exo-Check Array: A549 protein markers were characterised using a commercially sourced Exo-Check Exosome Antibody Array kit (#EXORAY200A-4, System Biosciences, CA, USA). A549 EVs (100 μg ...
-
bioRxiv - Systems Biology 2020Quote: ... GFP gene was amplified from pGreenPuro shRNA Cloning and Expression Lentivector (CMV; System biosciences, SI505A-1) and sub-cloned into ITR-CAG-DEST-IRES-Neomycin-ITR plasmid (generous gift from Marc Supprian Schmidt) ...
-
bioRxiv - Neuroscience 2021Quote: ... The viral particles were precipitated from the media using AAVanced concentration reagent (System Biosciences #AAV100A-1) according to manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... in DMEM medium supplemented with 10% FBS depleted of exosomes (System Biosciences, EXO-FBS-250A-1). Cells were maintained in this medium for 24 h before supernatant collection for EV purification ...
-
bioRxiv - Immunology 2021Quote: ... A PEG-it Virus Precipitation Solution (Cat# LV810A-1, System Biosciences, Palo Alto, CA 94303, USA) was added to 40ml of virus culture at a 1:4 ratio ...
-
bioRxiv - Cancer Biology 2022Quote: ... Media containing virus particles was mixed with PEG-it virus precipitation solution (System Biosciences, #LV810A-1) and incubated at 4°C overnight ...
-
bioRxiv - Cell Biology 2022Quote: ... These constructs are based on an empty parent minicircle cloning vector (MN100A-1; System Biosciences (SBI), Palo Alto ...
-
bioRxiv - Neuroscience 2022Quote: ... supernatants were incubated with 120 μL ExoQuick Exosome Precipitation solution (System Biosciences, Inc.; Catalog # EXOQ5TM-1) for 30 min at 4°C ...
-
bioRxiv - Microbiology 2022Quote: ... supernatant was diluted (4 to 1 ratio v/v) with PEG-it solution (SBI, System Biosciences), vortexed and incubated continuously rotating overnight at 4°C ...
-
bioRxiv - Developmental Biology 2022Quote: ... The CRISPR gRNA (5pg per embryo) and Cas9 mRNA (150pg per embryo, System Biosciences CAS500A-1) were injected into single-cell embryos ...
-
bioRxiv - Molecular Biology 2023Quote: ... the SpyCas9-ABE-tracrRNA PiggyBac transposon plasmid and PiggyBac transposase expression plasmid (System Biosciences PB210PA-1) were mixed in a 2:1 molar ratio and transfected into the HEK293T-dGFP reporter cell line using Lipofectamine 3000 (ThermoFisher L3000015) ...
-
bioRxiv - Cancer Biology 2023Quote: EVs from biological fluids were isolated using ExoQuick exosome precipitation solution (System Biosciences, SBI-EXOQ5A-1). Plasma and serum samples were centrifuged at 3,000 × g for 15 min to remove cells and cell debris ...
-
bioRxiv - Cancer Biology 2023Quote: ... A second ESR1-DAB2 plasmid in a pCDH-CMV-MCS-ER1α-Puro (System Biosciences #CD510B-1) was received as a kind gift from Dr ...
-
bioRxiv - Molecular Biology 2024Quote: ... Lentiviruses were generated by transfecting the plasmids using pPACKH1 Packaging Plasmid Mix (LV500A-1; System Biosciences) into a 293T cell line (LV900A-1 ...
-
Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... cells were transfected with 0.2 μg of Super PiggyBac Transposase Expression Vector (System Biosciences, PB210PA-1) and 0.2 ...
-
bioRxiv - Molecular Biology 2019Quote: The identification of protein markers on the isolated exosomes was undertaken using the commercially available Exo-Check Exosome Ab Array kit (System Biosciences, Palo Alto, CA), as described by the manufacturer ...
-
bioRxiv - Cancer Biology 2021Quote: ... The transfer vector was then packaged into a lentivirus using the pPACKH1 HIV third generation lentiviral expression system kit and PureFection reagent (both from System Biosciences, Palo Alto, CA) into HEK293-T cells (ATCC ...
-
bioRxiv - Cancer Biology 2023Quote: EV pellets were diluted and 45 µL was examined with the ExoELISA-ULTRA CD63 Kit according to the manufacturer’s instructions (System Biosciences, Palo Alto, CA, USA). The sample absorbance minus the blank wells was used to determine concentrations based on the standard curve included in the kit.
-
Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... We created the PB transposase plasmid pSPBase by removing the ccdB gene from PB210PA-1 (System Biosciences) to render it generally propagable ...
-
bioRxiv - Genetics 2021Quote: ... An in vitro transcribed sgRNA targeting Slc16a11 (5’-AGTCCTAACCTCGCTTGGCT-3’) and hspCas9 mRNA (CAS500A-1, System Biosciences) were microinjected into C57BL/6J mouse zygotes by the Genome Modification Facility at Harvard University ...
-
bioRxiv - Cell Biology 2021Quote: ... MNT1 cells were transfected with Pb-TRE3G-SpCas9-IRES-Blast and piggyBac transposase (PB210PA-1, System Biosciences) in 1:3 ratio using lipofectamine (Life Technologies ...
-
bioRxiv - Microbiology 2020Quote: ... cDNA sequences were cloned into the PB-CMV-MCS-EF1α-Puro vector (PB510B-1, SBI System Biosciences) where the CMV promoter was replaced by a PGK promoter ...
-
bioRxiv - Genomics 2021Quote: ... was stably introduced in HEK293T cells using the piggy-transposase system (System Biosciences, cat. no. PB210PA-1) according to the manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2021Quote: ... the gfp-lem8 fusion was inserted into pCDH-CMV-MCS-EF1a-Puro (System Biosciences, cat# CD510B-1). The plasmids were co-transfected with pMD2.G (gift from Dr ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: We used the human iPSC lines mciPS (model no. SC301A-1; System Biosciences, Palo Alto, CA, USA), SBAD2 [75] ...
-
bioRxiv - Developmental Biology 2022Quote: ... freshly dissociated brain cells were first transduced with lentivirus delivering human SNIP1 transgene (System Biosciences CD823A-1) in ultra-low attachment 6-well plates ...
-
bioRxiv - Genomics 2020Quote: ... multiple viral supernatants were collected and concentrated using PEG-it Virus Precipitation Solution (System Biosciences LV810A-1). Cells were infected 2-3 times at 12-hour intervals before selection in the appropriate antibiotic.
-
bioRxiv - Cell Biology 2020Quote: ... collected after 24 hours and concentrated using Peg-It Virus precipitation solution (cat# LV810A-1, System Biosciences) according to the manufacturer’s manual.
-
bioRxiv - Immunology 2024Quote: ... The HEK293TN-hACE2 cell line was generated by lentiviral transduction of HEK293TN (System Bioscience, Cat#LV900A-1) cells as described in Notarbartolo S ...
-
bioRxiv - Cancer Biology 2020Quote: Exosomes were purified from cultured medium supplemented with exosome-depleted FBS using the exosome purification kit following manufacturer’s instructions (ExoQuick™, System Bioscience, Mountain View, CA, USA).
-
bioRxiv - Molecular Biology 2020Quote: ... we replaced the KRAB and dCas9 cassettes from the pHR-SFFV-KRAB-dCas9-mCherry vector with the HA-tag-MEOX1 cassette using Cold-fusion cloning kit (SBI System BioSciences, Palo Alto, CA, USA) following the instruction provided by the manufacturer ...
-
bioRxiv - Developmental Biology 2022Quote: ... with 250 μg protein equivalent of AFSC-EVs stained using 2 μL of ExoGlow™-Vivo (Near IR) EV Labeling Kit (System Biosciences, Palo Alto, CA).
-
bioRxiv - Cell Biology 2021Quote: ... Cas9-MNT1 cells were transfected with Pb459-U6-sgRNA-EF1a-PuroR and piggyBac transposase (PB210PA-1, System Biosciences) in a 1:3 ratio using lipofectamine (Life Technologies ...