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Citations for System Biosciences :
101 - 147 of 147 citations for Dengue Virus Serotype 2 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 1 million cells were co-transfected with 1 µg ePiggyBac transposase expression plasmid (System Biosciences) and 1 µg each epB-MCP-mScarletI ...
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bioRxiv - Bioengineering 2023Quote: HEK293T cells stably expressing mRNA constructs were generated using the ϕC31 integrase system (System Biosciences) according to the manufacturers protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified viral particles were transduced into MIN6 cells using TransDux MAX (LV860A-1; System Biosciences) and cultured for 48-72 hours in complete DMEM (11965-092 ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... cells were transfected with 0.2 μg of Super PiggyBac Transposase Expression Vector (System Biosciences, PB210PA-1) and 0.2 ...
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bioRxiv - Developmental Biology 2024Quote: ... The expression vector coding GCaMP6s was introduced into MDCK cells with Super PiggyBac transposase (System Bioscience). After a few days ...
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bioRxiv - Immunology 2019Quote: ... and used to transduce pre-activated primary human CD4+ T cells in combination with Transdux (System Biosciences). GFP+ cells were FACS sorted 48h later ...
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bioRxiv - Cell Biology 2021Quote: ... The vectors were transduced into HeLa cells using the PiggyBac transposon system (System Biosciences, Palo Alto, CA), and the cells were selected for 2 weeks with 2 μg/ml of puromycin ...
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bioRxiv - Cell Biology 2021Quote: ... MNT1 cells were transfected with Pb-TRE3G-SpCas9-IRES-Blast and piggyBac transposase (PB210PA-1, System Biosciences) in 1:3 ratio using lipofectamine (Life Technologies ...
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bioRxiv - Genomics 2021Quote: ... was stably introduced in HEK293T cells using the piggy-transposase system (System Biosciences, cat. no. PB210PA-1) according to the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2022Quote: ... freshly dissociated brain cells were first transduced with lentivirus delivering human SNIP1 transgene (System Biosciences CD823A-1) in ultra-low attachment 6-well plates ...
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bioRxiv - Genomics 2022Quote: THP-1 cells were transduced in triplicates using the TransDux MAX transfection reagent (System Biosciences, LV860A-1) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2020Quote: ... All other 293T-GFP responder cell lines were constructed by lentiviral transduction (pCDH vector backbone, System Biosciences). All transient expression plasmids were cloned into the pCS2+ vector using standard restriction cloning ...
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bioRxiv - Immunology 2024Quote: ... The HEK293TN-hACE2 cell line was generated by lentiviral transduction of HEK293TN (System Bioscience, Cat#LV900A-1) cells as described in Notarbartolo S ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lines were immediately cryopreserved and maintained in selection medium containing 60 μg/mL cumate (System Biosciences) for at least one passage prior to assay.
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bioRxiv - Cell Biology 2021Quote: ... Cas9-MNT1 cells were transfected with Pb459-U6-sgRNA-EF1a-PuroR and piggyBac transposase (PB210PA-1, System Biosciences) in a 1:3 ratio using lipofectamine (Life Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviruses containing the luciferase expression cassette were produced in 293 TN cells (System Bioscience, catalog number: LV900A-1) by transient transfection with the PSMPUW-Hygro lentiviral vector carrying the luciferase gene and the ViraSafe™ Lentiviral Packaging System (Cell Biolabs ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmids for stable cell line construction were generated by Gibson cloning into pCDH-EF1-CymR-T2A-Neo (System Bioscience). Sequences of all plasmids were confirmed by Sanger sequencing.
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bioRxiv - Molecular Biology 2020Quote: The Jurkat cell transductions were done with multiple viral titers using TransDux™ MAX Lentivirus Transduction Reagent (System Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Stable cell lines were generated by cotransfection with the Super PiggyBac Transposase Expression Vector (cat# PB210PA-1, System Biosciences) followed by selection via addition of 5 μg/mL puromycin (cat# ant-pr-1 ...
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bioRxiv - Bioengineering 2023Quote: Stable HeLa-Fucci cell line was generated using the Super PiggyBac Transposase Expression Vector system (System Biosciences, PB210PA-1) together with tFucci(CA)5 plasmid29 (Addgene #153521) ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmids for stable cell line construction were generated by Gibson cloning into pCDH-EF1-CymR-T2A-Neo (System Bioscience) vector ...
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bioRxiv - Biophysics 2023Quote: ... Cells were transfected with 1500ng of HaloTag-NLS or HaloTag-H2b + 1000ng of piggyBac Transposase (System Biosciences, PB210PA-1) using Lipofectamine2000 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: Cells were cultured in DMEM/1640 supplemented with 10% exosome-depleted FBS (EXO-FBS-250A-1, SBI System Biosciences, USA) using 10×10 cm2 disks (1×106 cells per disk ...
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bioRxiv - Genetics 2019Quote: To produce lentiviral cell lines containing pGreenFire1™ Pathway Reporter lentivector (Cat#TR010PA-N and Cat#TR000PA-1, System Bioscience) expressing the MUC5B promoter region and controls ...
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bioRxiv - Cancer Biology 2024Quote: ... stable HEK293-F cell lines for each cytokine were prepared through cloning into the expression cassette of PiggyBac (System Biosciences) transposon vector ...
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bioRxiv - Molecular Biology 2021Quote: The primary human T cell transductions were done with multiple viral titers using TransDux™ MAX Lentivirus Transduction Reagent (System Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... with the respective lamin containing or empty (Mock) vectors into HEK 293-TN cells using PureFection nanotechnology-based transfection reagent (System Biosciences). The medium was exchanged the next day and replaced by antibiotics-free DMEM containing 10% FCS ...
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bioRxiv - Cell Biology 2021Quote: TYR-G1-G4 knockout cell lines were transfected with the Pb-TRE3G-TYR-HygR plasmid and piggyBac transposase (PB210PA-1, System Biosciences) in a 1:3 ratio using lipofectamine (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... NK92 CD56-KO cells were transduced with pBabe-puro-NCAM mApple viral particles in the presence of MAX Enhancer and TransDux (System Biosciences). Within 48 hours cells were sorted for CD56 and mApple and cells were expanded and maintained under antibiotic selection with regular phenotypic validation.
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bioRxiv - Microbiology 2020Quote: The 3’UTR reporter plasmids were co-transfected into HEK293T cells using PEI max together with the indicated pCDH-EF1-MCS expressing plasmids (System Biosciences) containing a cloned pre-miRNA gene of interest ...
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bioRxiv - Immunology 2022Quote: ... encoding HLA-A*02:01 or HLA-A*03:01 by spinoculation with ultracentrifuge concentrated viral particles produced from 293T-HEK-TN cells (System biosciences), followed by sorting of cells stained with anti-human HLA-A ...
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bioRxiv - Developmental Biology 2019Quote: We acquired 6 human induced pluripotent stem cell (iPSC) lines (Sojd3, Hoik1, Kucg2, Wibj2 from the HipSci resource54; h409b2 from the RIKEN BRC cell bank17; Sc102a1 from System Biosciences), one human ES cell line (H9 ...
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bioRxiv - Bioengineering 2023Quote: ... The in vivo T cell activation reporter was constructed by cloning a sequence containing firefly luciferase into the pGreenFire1-NF-κB lentiviral vector (System Biosciences) under the NF-κB responsive promoter ...
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bioRxiv - Bioengineering 2023Quote: ... were purchased from the Riken Brain Science Institute (Japan) and used to generate lentivirus particles by co-transfecting HEK 293TN cells (System Biosciences) with packaging (psPAX2 ...
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bioRxiv - Cell Biology 2024Quote: Stable cell lines expressing GFP-tagged cytoplasmic tail chimeras under a cumate-inducible promoter were generated by PiggyBac transposition (System Biosciences). Wild-type ...
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bioRxiv - Molecular Biology 2021Quote: ... Frozen primary human T cell pellets were thawed and then stimulated with ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (Stemcell) for 48 hours and then transduced with various CAR viruses using TransDux™ MAX Lentivirus Transduction Reagent (System Biosciences) as described above ...
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bioRxiv - Cell Biology 2024Quote: ... Extracellular vesicles were isolated from 10 mL of cell culture supernatant using the ExoQuick-TC® Exosome Precipitation Solution (cat# EXOTC10A-1; System Biosciences) according to the manufacturer’s instructions with a few modifications ...
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bioRxiv - Microbiology 2021Quote: ... EBV-negative Akata(-) cells were treated with purified exosomes in RPMI1640 supplemented with 5% exosome-depleted FBS (System Biosciences, Palo Alto, CA, USA). After 6 h ...
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bioRxiv - Molecular Biology 2023Quote: ... in which A375 Cas9 cells were co-transfected with the rescue plasmids as well as the Super PiggyBac Transposase Expression Vector (System Bioscience Inc, PB200PA) at a 4:1 ratio utilising Lipofectamine™ LTX reagents (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... 6 X 105 cells were co-transfected with 5 µg of gRNA-containing piggyBac vector and 1 µg of piggyBac transposase plasmid (System Biosciences PB210PA-1) using Lipofectamine 3000 Transfection Reagent ...
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bioRxiv - Cell Biology 2022Quote: Bare 293T cells were first co-transfected using PEI47 with the H2B-mTagBFP2 vector and Super PiggyBac Transposase-expressing vector (System Biosciences Inc. # PB210PA-1) via polyethylenimine (Sigma #408727-100mL ...
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bioRxiv - Cancer Biology 2020Quote: ... MDA-MB-231 and MDA-MB-231-Luc+ cells were transduced with lentivirus vector expressing a GFP or RFP tag fused to the membrane-associated tetraspanin CD63 (System Biosciences, Palo Alto, CA). Breast cancer cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cancer Biology 2023Quote: ... E0771 and RM-1 cells were transduced with lentiviral vectors carrying the luciferase expression cassette with TransDux MAXTM (System Bioscience, catalog number: LV860A-1). Luciferase-labeled E0771 and RM-1 cells were then selected in the presence of 300 µg/mL hygromycin ...
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bioRxiv - Biochemistry 2021Quote: ... coli minicircle producer strain24 competent cells (Cat. No. MN900A-1) and Arabinose Induction Solution (Cat. No. MN850A-1) were purchased from System Biosciences (Palo Alto, CA, USA). The luciferase-expressing ...
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bioRxiv - Biochemistry 2021Quote: MDA-MB-231 or HEK 293T cells were grown to ~80% confluency in 14 × 150 mm dishes with 10% exosome-depleted FBS (Exo-FBS from System Biosciences, EXO-FBS-250A-1) in DMEM GlutaMAX media ...
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bioRxiv - Microbiology 2020Quote: ... HEK293T cells (ATCC® ACS-4500™) were transiently transfected with hACE2 fusion BFP encoding PB510 plasmid using PiggyBac Transposon System (System Biosciences, PB210PA-1), followed by addition of 2 μg/mL puromycin 6 h post-transfection ...
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bioRxiv - Microbiology 2021Quote: ... isolate USA-WA1/2020) was propagated in Vero cells and concentrated with a polyethylene glycol (PEG) (Cat# LV-810A, System Biosciences, Palo Alto, CA 94303, USA) to a titer of ~1×107 plaque forming units (PFU)/ml ...