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Citations for System Biosciences :
101 - 150 of 248 citations for 7 Phenyl 1 heptanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 0.25ml of cold PEG-it Virus Precipitation Solution (LV810A-1-SBI, System Biosciences) was added per 1ml of lentiviral-containing supernatant and kept cool at 4°C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... we used the PCDF1-MCS2-EF1-Puro expression vector (System Biosciences, CD110B-1). We performed a restriction digest and gel extraction as above to the plasmid ...
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bioRxiv - Neuroscience 2024Quote: ... and PEG-It Virus Precipitation Solution (cat # LV825A-1) was from System Biosciences. Macrophage colony stimulating factor (M-CSF ...
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bioRxiv - Molecular Biology 2021Quote: ... This plasmid was co-transfected with piggyBac Transposase expression vector (System Biosciences, PB210PA-1) into the PABPC1-AID cell line (sC152-C16) ...
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bioRxiv - Bioengineering 2020Quote: ... viral supernatant was concentrated using PEG-it virus precipitation solution (System Biosciences LV825A-1) according to manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... Ceg3 was inserted into pCDH-CMV-MCS-EF1a-RFP (System Biosciences cat# CD512B-1), which was transfected together with pMD2.G (Addgene plasmid #12259 ...
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bioRxiv - Cell Biology 2020Quote: The transfection of FAPs with pCT-CD63-GFP plasmid (#CYTO120-PA-1, System Biosciences) and with a GFP-control vector (mock ...
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bioRxiv - Molecular Biology 2022Quote: ... EVs were isolated using ExoQuick-TC (EXOTC50A-1, System Biosciences, Palo Alto, CA, USA) according to manufacturer instructions ...
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bioRxiv - Genetics 2019Quote: ... We transfected the plasmids with Super PiggyBac Transposase Expression Vector (System Biosciences, PB210PA-1) using Lipofectamine™ 3000 Transfection Reagent ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and ligated into the CAGs-MCS-Enhanced Episomal Vector (EEV) (System Biosciences, EEV600A-1) linearized with AgeI and KpnI ...
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bioRxiv - Immunology 2023Quote: ... pCDHv6_PTPRZ1 (derived from pCDH-MSCV-MCS-EF1-GFP-T2A-Puro, System Biosciences, CD713B-1), with packaging plasmids pMDLg/pRRE (RRID:Addgene_12251) ...
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bioRxiv - Neuroscience 2022Quote: ... and microglial enrichment was conducted per manufacturer’s instructions (System Biosciences, Inc.; Catalog # CSFLOWBASICA-1). Briefly ...
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bioRxiv - Genetics 2023Quote: ... cells were transfected with 0.72 μg of piggyBac transposase plasmid (System Biosciences PB210PA-1) and 1.78 μg of pCX4.1 plasmid using Lipofectamine 3000 transfection reagent ...
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bioRxiv - Cell Biology 2023Quote: ... and HEK 293TN (LV900A-1) cells were purchased from System Biosciences (Palo Alto, USA). Mouse mesothelial cells (MCs ...
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bioRxiv - Cell Biology 2024Quote: ... the piggyBac expression vector and Super PiggyBac transposase expression vector (PB210PA-1, System Bioscience) were introduced into the cells using a 2-step pulse electroporator (NEPA21 ...
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bioRxiv - Cancer Biology 2024Quote: CRC cells were infected using Human Nanog pGreenZeo Differentiation Reporter (System Biosciences SR10030VA-1) (www.systembio.com ...
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bioRxiv - Bioengineering 2021Quote: ... RFP-expressing GBM12 (GBM12-RFP) was generated by transduction with lentivirus (System Biosciences, LL110VA-1) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2020Quote: ... EV depleted FCS was either commercially obtained (System Biosciences Catalog no. EXO-FBS-250A-1) or prepared by ultracentrifugation of the FCS used at 120,000 x g for 5 hours ...
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bioRxiv - Microbiology 2020Quote: ... Fusion was performed with the Cold Fusion Cloning Kit (SBI System Biosciences, Cat. #MC010B-1).
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bioRxiv - Cell Biology 2021Quote: Inducible Cas9 iPSCs were generated using an AAVS1 TALE-Nuclease KIT (GE60xA-1; System Biosciences) and an iCas9 HR donor plasmid containing a TRE3G promoter-driven spCas9 with a C-terminal MYC NLS followed by a CMV promoter-driven Tet-On-3G and a neomycin resistance adapted from Ihry et al ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified viral particles were transduced into MIN6 cells using TransDux MAX (LV860A-1; System Biosciences) and cultured for 48-72 hours in complete DMEM (11965-092 ...
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bioRxiv - Immunology 2024Quote: ... The PCR fragments were cloned into a PiggyBac mammalian expression vector (System Biosciences, PB210PA-1), modified to harbor a Secrecon-AA signal peptide111 for secretion at the N-terminus ...
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bioRxiv - Cell Biology 2023Quote: ... from 3T3-L1 genomic DNA and cloned into the HR110-PA-1 vector (System Biosciences). Primer sequences to amplify the 5′- and 3′-side genomic fragments for homologous recombination are as follows ...
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bioRxiv - Cancer Biology 2023Quote: 2.5×106 IFU/ml of pooled lenti-miRNA lentivirus library (System Biosciences Cat. #PMIRHPLVHT-1) was mixed with 4 mg/mL polybrene (Sigma ...
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bioRxiv - Physiology 2023Quote: ... and 5 µL of de-fibrination reagent (Cat #TMEXO-1, System Biosciences, Palo Alto, CA) was added to the plasma to remove clotting factors prior to the isolation of EVs ...
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bioRxiv - Systems Biology 2020Quote: ... GFP gene was amplified from pGreenPuro shRNA Cloning and Expression Lentivector (CMV; System biosciences, SI505A-1) and sub-cloned into ITR-CAG-DEST-IRES-Neomycin-ITR plasmid (generous gift from Marc Supprian Schmidt) ...
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bioRxiv - Neuroscience 2021Quote: ... The viral particles were precipitated from the media using AAVanced concentration reagent (System Biosciences #AAV100A-1) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... in DMEM medium supplemented with 10% FBS depleted of exosomes (System Biosciences, EXO-FBS-250A-1). Cells were maintained in this medium for 24 h before supernatant collection for EV purification ...
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bioRxiv - Immunology 2021Quote: ... A PEG-it Virus Precipitation Solution (Cat# LV810A-1, System Biosciences, Palo Alto, CA 94303, USA) was added to 40ml of virus culture at a 1:4 ratio ...
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bioRxiv - Cancer Biology 2022Quote: ... Media containing virus particles was mixed with PEG-it virus precipitation solution (System Biosciences, #LV810A-1) and incubated at 4°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... These constructs are based on an empty parent minicircle cloning vector (MN100A-1; System Biosciences (SBI), Palo Alto ...
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bioRxiv - Neuroscience 2022Quote: ... supernatants were incubated with 120 μL ExoQuick Exosome Precipitation solution (System Biosciences, Inc.; Catalog # EXOQ5TM-1) for 30 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... supernatant was diluted (4 to 1 ratio v/v) with PEG-it solution (SBI, System Biosciences), vortexed and incubated continuously rotating overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... The CRISPR gRNA (5pg per embryo) and Cas9 mRNA (150pg per embryo, System Biosciences CAS500A-1) were injected into single-cell embryos ...
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bioRxiv - Molecular Biology 2024Quote: ... Lentiviruses were generated by transfecting the plasmids using pPACKH1 Packaging Plasmid Mix (LV500A-1; System Biosciences) into a 293T cell line (LV900A-1 ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... cells were transfected with 0.2 μg of Super PiggyBac Transposase Expression Vector (System Biosciences, PB210PA-1) and 0.2 ...
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bioRxiv - Molecular Biology 2023Quote: ... the SpyCas9-ABE-tracrRNA PiggyBac transposon plasmid and PiggyBac transposase expression plasmid (System Biosciences PB210PA-1) were mixed in a 2:1 molar ratio and transfected into the HEK293T-dGFP reporter cell line using Lipofectamine 3000 (ThermoFisher L3000015) ...
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bioRxiv - Cancer Biology 2023Quote: EVs from biological fluids were isolated using ExoQuick exosome precipitation solution (System Biosciences, SBI-EXOQ5A-1). Plasma and serum samples were centrifuged at 3,000 × g for 15 min to remove cells and cell debris ...
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bioRxiv - Cancer Biology 2023Quote: ... A second ESR1-DAB2 plasmid in a pCDH-CMV-MCS-ER1α-Puro (System Biosciences #CD510B-1) was received as a kind gift from Dr ...
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bioRxiv - Cell Biology 2024Quote: ... Exosome isolation from mouse serum was performed using an ExoQuick precipitation reagent (System Biosciences, EXOQ5A-1) and a Fixed-Angle Rotor TLA120.1 (Beckman Coulter ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... We created the PB transposase plasmid pSPBase by removing the ccdB gene from PB210PA-1 (System Biosciences) to render it generally propagable ...
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bioRxiv - Genetics 2021Quote: ... An in vitro transcribed sgRNA targeting Slc16a11 (5’-AGTCCTAACCTCGCTTGGCT-3’) and hspCas9 mRNA (CAS500A-1, System Biosciences) were microinjected into C57BL/6J mouse zygotes by the Genome Modification Facility at Harvard University ...
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bioRxiv - Cell Biology 2021Quote: ... MNT1 cells were transfected with Pb-TRE3G-SpCas9-IRES-Blast and piggyBac transposase (PB210PA-1, System Biosciences) in 1:3 ratio using lipofectamine (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... cDNA sequences were cloned into the PB-CMV-MCS-EF1α-Puro vector (PB510B-1, SBI System Biosciences) where the CMV promoter was replaced by a PGK promoter ...
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bioRxiv - Genomics 2021Quote: ... was stably introduced in HEK293T cells using the piggy-transposase system (System Biosciences, cat. no. PB210PA-1) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... the gfp-lem8 fusion was inserted into pCDH-CMV-MCS-EF1a-Puro (System Biosciences, cat# CD510B-1). The plasmids were co-transfected with pMD2.G (gift from Dr ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: We used the human iPSC lines mciPS (model no. SC301A-1; System Biosciences, Palo Alto, CA, USA), SBAD2 [75] ...
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bioRxiv - Developmental Biology 2022Quote: ... freshly dissociated brain cells were first transduced with lentivirus delivering human SNIP1 transgene (System Biosciences CD823A-1) in ultra-low attachment 6-well plates ...
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bioRxiv - Genomics 2020Quote: ... multiple viral supernatants were collected and concentrated using PEG-it Virus Precipitation Solution (System Biosciences LV810A-1). Cells were infected 2-3 times at 12-hour intervals before selection in the appropriate antibiotic.
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bioRxiv - Cell Biology 2020Quote: ... collected after 24 hours and concentrated using Peg-It Virus precipitation solution (cat# LV810A-1, System Biosciences) according to the manufacturer’s manual.