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Citations for System Biosciences :
51 - 71 of 71 citations for Poloxamer 188 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... Viral supernatants were collected 48 hours-post transfection and passed through a 0.4 μm followed by overnight incubation with 1X PEG-IT solution (System Biosciences) at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... 100 ml of LB broth together with 100 µl of 20% arabinose induction solution (System Biosciences, Palo Alto, CA, USA) and 4 ml of 1N NaOH was added to the culture and grown for 5.5 hrs at 30°C ...
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bioRxiv - Biochemistry 2020Quote: ... The serum (50-100 μl) was treated with ExoQuick Exosome Precipitation Solution (25.2 μl/100 μl serum; System Biosciences, CA) on ice for 30 min ...
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bioRxiv - Biophysics 2022Quote: ... Lentiviral libraries were prepared as described above in complete DMEM and harvested using PEG-it Virus Precipitation Solution (System Biosciences). Lentiviral titers were checked using the hFzd5CRD-1TM cells at 2 ml scale based on Wnt expression ...
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bioRxiv - Neuroscience 2022Quote: ... The lentivirus was concentrated with the use of the PEG-it virus precipitation solution (2.5 ml, System Biosciences, LV810A-1) for 2-3 days at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... EVs were isolated from tissue culture media using a commercial kit containing a polyethylene glycol (PEG) solution (ExoQuick-TC ULTRA kit, EQULTRA-20TC-1; System Biosciences) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Culture medium containing the shed virus was collected at 72 hours and incubated with PEG-it virus precipitation solution (System Biosciences) at 4°C for 48 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Lentiviral particles were harvested either by ultracentrifugation at 106,559 g for 2 h at 4°C using a Beckman SW 32 Ti rotor or using the PEG-it Virus Precipitation Solution (System Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Medium containing lentiviral particles was collected at 48 and 72 hours post transfection and concentrated using PEG-it Virus Precipitation Solution (System BioSciences). Precipitated lentiviral particles were resuspended in DMEM ...
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bioRxiv - Molecular Biology 2024Quote: ... and with the cloned gRNA/barcode/target sequences were generated using the Broad Institute’s RNAi Consortium protocol (https://portals.broadinstitute.org/gpp/public/dir/download?dirpath=protocols/production&filename=TRC%20shRNA%20sgRNA%20ORF%20Low%20Throughput%20Viral%20Production%20201506.pdf) for low-throughput lentiviral production and then concentrated using PEG-it virus precipitation solution (System Biosciences) per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The secreted EVs were partially purified from cultured medium by using ExoQuick-TC Exosome Precipitation Solution (20 μl/100 μl serum; System Biosciences, CA) at 4°C for overnight ...
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bioRxiv - Immunology 2022Quote: ... VLPs were precipitated from the clarified supernatant by the addition of PEG-it Virus Precipitation Solution (System Biosciences, Palo Alto, CA, USA) at a ratio of 1:10 ...
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bioRxiv - Molecular Biology 2024Quote: ... The pooled supernatant was then filtered with 0.45 μm membrane and concentrated using the PEG-it virus precipitation solution (System Biosciences #LV810A-1) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Extracellular vesicles were isolated from 10 mL of cell culture supernatant using the ExoQuick-TC® Exosome Precipitation Solution (cat# EXOTC10A-1; System Biosciences) according to the manufacturer’s instructions with a few modifications ...
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bioRxiv - Genomics 2023Quote: ... The lentivirus-containing media was mixed at a 1:4 ratio of PEG-it virus precipitation solution (System Biosciences, Cat. No. LV810A-1) to media ...
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bioRxiv - Cancer Biology 2021Quote: ... Media containing virus was collected both 48 and 72 hours post-transfection and viral particles were precipitated using PEG-it™ Virus Precipitation Solution (System Biosciences, LV810A-1). To transduce cells with virus cells were treated with both virus (MOI 0.5-1.0 ...
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bioRxiv - Cancer Biology 2022Quote: ... Media was collected at 48 and 72 hours post-transfection and viral particles were precipitated using PEG-it™ Solution (System Biosciences, LV810A-1). To transduce ...
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bioRxiv - Cell Biology 2022Quote: ... IL) lentiviruses were grown in human embryonic kidney 293T cells and concentrated using the PEG-it Virus Precipitation Solution (System Biosciences, Mountain View, CA), as previously described (51) ...
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bioRxiv - Molecular Biology 2024Quote: ... Media was collected at 48 and 72 hours post-transfection and viral particles were precipitated using PEG-it™ Solution (System Biosciences, LV810A-1). To transduce ...
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bioRxiv - Biochemistry 2021Quote: ... coli minicircle producer strain24 competent cells (Cat. No. MN900A-1) and Arabinose Induction Solution (Cat. No. MN850A-1) were purchased from System Biosciences (Palo Alto, CA, USA). The luciferase-expressing ...
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bioRxiv - Cell Biology 2021Quote: ... Virus-containing supernatants were either collected or concentrated by mixing with quarter volumes of 5 × PEG-it Virus Precipitation Solution (System Biosciences, Palo Alto, CA, United States), followed by centrifugation according to the manufacturer’s protocol ...