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Citations for System Biosciences :
51 - 94 of 94 citations for PEG NH2 modified upconverting red light since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... collected after 24 hours and concentrated using Peg-It Virus precipitation solution (cat# LV810A-1, System Biosciences) according to the manufacturer’s manual.
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bioRxiv - Molecular Biology 2023Quote: ... filtered using 45 um filters and concentrated 100-fold using PEG-it Virus Precipitation Solution (System Biosciences).
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bioRxiv - Molecular Biology 2024Quote: ... the filtered supernatant underwent a 100-fold concentration step using PEG-it Virus Precipitation Solution (System Biosciences), according to the manufacturer’s protocols.
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bioRxiv - Immunology 2024Quote: ... Viruses were then concentrated overnight at 4°C using the PEG- it virus precipitation solution (System Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Lentivirus was generated with PEG-it Virus Precipitation Solution (System Biosciences, Palo Alto, CA, USA no LV810A-1) using a LV H2B-GFP plasmid (Addgene no 25999 ...
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bioRxiv - Cancer Biology 2023Quote: ... Viral particles were harvested 2 days later and concentrated with Peg-itTM (System Biosciences, Palo Alto, CA, USA).
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bioRxiv - Immunology 2024Quote: ... sterile filtered through a 0.45-μm syringe filter and concentrated using PEG-it Virus Precipitation Solution (System Biosciences). Virus was then aliquoted and stored at −80°C ...
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bioRxiv - Genomics 2023Quote: ... The collected virus was concentrated 100-fold after precipitation with PEG-itTM Virus Precipitation Solution (System BioSciences LV810A-1) for 3 days ...
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bioRxiv - Cancer Biology 2023Quote: ... The viruses from each time point were pooled and concentrated using PEG-it virus precipitation solution (System Biosciences, LV810A) according to manufacture’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Viral supernatants were collected 48 hours-post transfection and passed through a 0.4 μm followed by overnight incubation with 1X PEG-IT solution (System Biosciences) at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... Lentiviral libraries were prepared as described above in complete DMEM and harvested using PEG-it Virus Precipitation Solution (System Biosciences). Lentiviral titers were checked using the hFzd5CRD-1TM cells at 2 ml scale based on Wnt expression ...
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bioRxiv - Neuroscience 2022Quote: ... The lentivirus was concentrated with the use of the PEG-it virus precipitation solution (2.5 ml, System Biosciences, LV810A-1) for 2-3 days at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... EVs were isolated from tissue culture media using a commercial kit containing a polyethylene glycol (PEG) solution (ExoQuick-TC ULTRA kit, EQULTRA-20TC-1; System Biosciences) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Culture medium containing the shed virus was collected at 72 hours and incubated with PEG-it virus precipitation solution (System Biosciences) at 4°C for 48 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Lentiviral particles were harvested either by ultracentrifugation at 106,559 g for 2 h at 4°C using a Beckman SW 32 Ti rotor or using the PEG-it Virus Precipitation Solution (System Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Medium containing lentiviral particles was collected at 48 and 72 hours post transfection and concentrated using PEG-it Virus Precipitation Solution (System BioSciences). Precipitated lentiviral particles were resuspended in DMEM ...
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bioRxiv - Cell Biology 2023Quote: ... The 30 mL of lentiviral supernatant was pooled and concentrated to 1/100th of its original volume (300 µL) in DPBS using PEG-iT (System Biosciences), following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... and with the cloned gRNA/barcode/target sequences were generated using the Broad Institute’s RNAi Consortium protocol (https://portals.broadinstitute.org/gpp/public/dir/download?dirpath=protocols/production&filename=TRC%20shRNA%20sgRNA%20ORF%20Low%20Throughput%20Viral%20Production%20201506.pdf) for low-throughput lentiviral production and then concentrated using PEG-it virus precipitation solution (System Biosciences) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... VLPs were precipitated from the clarified supernatant by the addition of PEG-it Virus Precipitation Solution (System Biosciences, Palo Alto, CA, USA) at a ratio of 1:10 ...
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bioRxiv - Molecular Biology 2024Quote: ... The pooled supernatant was then filtered with 0.45 μm membrane and concentrated using the PEG-it virus precipitation solution (System Biosciences #LV810A-1) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and was cloned into a modified CD813A vector (System Biosciences) containing the beta-globin (HBB ...
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bioRxiv - Molecular Biology 2021Quote: ... and was cloned into a modified CD813A vector (System Biosciences) using the In-Fusion® HD Cloning Kit (Takara) ...
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bioRxiv - Genomics 2023Quote: ... The lentivirus-containing media was mixed at a 1:4 ratio of PEG-it virus precipitation solution (System Biosciences, Cat. No. LV810A-1) to media ...
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bioRxiv - Cancer Biology 2021Quote: ... Media containing virus was collected both 48 and 72 hours post-transfection and viral particles were precipitated using PEG-it™ Virus Precipitation Solution (System Biosciences, LV810A-1). To transduce cells with virus cells were treated with both virus (MOI 0.5-1.0 ...
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bioRxiv - Cancer Biology 2022Quote: ... Media was collected at 48 and 72 hours post-transfection and viral particles were precipitated using PEG-it™ Solution (System Biosciences, LV810A-1). To transduce ...
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bioRxiv - Cell Biology 2022Quote: ... IL) lentiviruses were grown in human embryonic kidney 293T cells and concentrated using the PEG-it Virus Precipitation Solution (System Biosciences, Mountain View, CA), as previously described (51) ...
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bioRxiv - Molecular Biology 2024Quote: ... Media was collected at 48 and 72 hours post-transfection and viral particles were precipitated using PEG-it™ Solution (System Biosciences, LV810A-1). To transduce ...
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bioRxiv - Cell Biology 2021Quote: ... The vector was modified from the PiggyBac vector PBQM812A-1 (System Biosciences) in which the IRES and downstream GFP were removed (John Shin) ...
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bioRxiv - Cell Biology 2021Quote: ... Virus-containing supernatants were either collected or concentrated by mixing with quarter volumes of 5 × PEG-it Virus Precipitation Solution (System Biosciences, Palo Alto, CA, United States), followed by centrifugation according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... isolate USA-WA1/2020) was propagated in Vero cells and concentrated with a polyethylene glycol (PEG) (Cat# LV-810A, System Biosciences, Palo Alto, CA 94303, USA) to a titer of ~1×107 plaque forming units (PFU)/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... and inserted into PB-CuO-MCS-BGH-EF1-CymR-Puro (modified from System Biosciences), together with an N-terminal FLAG-emGFP-tag via NheI and NotI (both New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... exosomes from ASCs were labeled fluorescent red using an ExoGlow-Protein EV Labeling Kit (System Biosciences) and then incubated with precipitation solution at 4°C overnight ...
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bioRxiv - Neuroscience 2022Quote: shRNAs targeting human HNRNPR were cloned into a modified version of pSIH-H1 shRNA vector (System Biosciences) containing EGFP according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... hFhl1 gene encoding FHL1A isoform fused with GFP was cloned into modified PiggyBac plasmid (System Bioscience, Inc) under control of the CMV promoter ...
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bioRxiv - Neuroscience 2022Quote: shRNAs targeting human HNRNPR were cloned into a modified version of pSIH-H1 shRNA vector (System Biosciences) containing EGFP according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... This vector was modified from the original vector (PB-CuO-MCS-IRES-GFP-EF1α-CymR-Puro (System Biosciences)) by replacing the IRES-GFP cassette with a BGH polyA signal ...
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bioRxiv - Biochemistry 2024Quote: ... This vector was modified from the original vector (PB-CuO-MCS-IRES-GFP-EF1α-CymR-Puro (System Biosciences)) by replacing the IRES- GFP cassette with a BGH polyA signal ...
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bioRxiv - Cell Biology 2024Quote: ... SapI sites surrounded the sORF sequences to enable cloning into the custom-modified pCDH_EF1_IRES_Puro lentiviral expression plasmid (System Biosciences, CD532A-2). Following golden gate cloning using SapI (NEB ...
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bioRxiv - Cancer Biology 2020Quote: ... Double-stranded oligonucleotides were cloned into the linearized SmartNuclease vector (EF1-T7-hspCas9-T2A-RFP-H1-gRNA with red fluorescent protein (RFP, System Biosciences) and IFNLR1-guide RNA (IFNLR1-gRNA ...
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bioRxiv - Microbiology 2020Quote: ... two modified PiggyBac (PB) transposon vectors (MIHIPsMie and EIRBsMie) were constructed based on PB-CMV-MCS-EF1α-RedPuro (System Biosciences, PB514B2). The fragment of CMV-MCS-EF1α-RedPuro on this vector was removed by SfiI/ApaI digestion ...
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bioRxiv - Neuroscience 2021Quote: ... shRNAs targeting mouse drebrin A and shRNAs targeting both drebrin A and E were cloned into a modified version of pSIH-H1 shRNA vector (System Biosciences) containing eGFP according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... U2-OS cells (UC Berkeley BDS Cell Culture Facility) cultured in DMEM (1 g/L glucose, 10% FBS) were transfected with a modified PiggyBac Transposase plasmid (System Biosciences) and a PGK.mNeonGreen-LacI.NLS.IRES.Puro integration plasmid using Fugene6 transfection reagent ...
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bioRxiv - Developmental Biology 2021Quote: ... The amplicon was cloned into a modified version of the self-inactivating lentiviral vector pPS-EF1α-GFP-RFP (System Biosciences, LV603PA-1) where GFP and RFP had been removed and replaced by an hPGK-Puromycin cassette (pSIN-EF1a promoter-BGH polyA-hPGK-Puromycin) ...
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bioRxiv - Bioengineering 2020Quote: ... these HEKs were genetically-modified reporter cells (NF-κB/293/GFP-Luc™ Transcriptional Reporter Cell Line; System Biosciences, catalogue number TR860A-I), but reporter activity is not relevant here.