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Citations for System Biosciences :
51 - 100 of 115 citations for Human MMP24 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The plasmid vector pCDH-EF1-MCS-T2A-copGFP was purchased from System Biosciences. The lentiviral vector was packaged in HEK293T cells as previously described ...
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bioRxiv - Genetics 2023Quote: ... previously created by substituting the copepod GFP from the pB530A plasmid (System Biosciences) with the Puromycin resistance gene ...
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bioRxiv - Molecular Biology 2021Quote: ... This plasmid was co-transfected with piggyBac Transposase expression vector (System Biosciences, PB210PA-1) into the PABPC1-AID cell line (sC152-C16) ...
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bioRxiv - Cell Biology 2021Quote: ... A lentiviral plasmid containing guide RNA sequences was transfected into 293TN cells (System Biosciences) using Lipofectamine 2000 transfection reagent ...
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bioRxiv - Cell Biology 2020Quote: The transfection of FAPs with pCT-CD63-GFP plasmid (#CYTO120-PA-1, System Biosciences) and with a GFP-control vector (mock ...
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bioRxiv - Genetics 2019Quote: ... We transfected the plasmids with Super PiggyBac Transposase Expression Vector (System Biosciences, PB210PA-1) using Lipofectamine™ 3000 Transfection Reagent ...
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bioRxiv - Bioengineering 2023Quote: ... The constructed donor plasmid was co-transfected with PiggyBac transposon vector (PB210PA, System Biosciences) into HEI-OC1 cells ...
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bioRxiv - Genetics 2023Quote: ... cells were transfected with 0.72 μg of piggyBac transposase plasmid (System Biosciences PB210PA-1) and 1.78 μg of pCX4.1 plasmid using Lipofectamine 3000 transfection reagent ...
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bioRxiv - Microbiology 2021Quote: ... Vero E6 and Huh7.5 cells with PiggyBAC/ACE2 and integrase-encoding plasmids (System Biosciences, Inc.) using Lipofectamine 3000 ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids used to produce GFP-lentiviruses were the lentivector backbone pCDH-EF1α-GFP (System Biosciences), the packaging plasmid psPAXII (Addgene) ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid was then packaged into viral particles using the pPACKH1 lentivector Packaging Kit (System Bioscience) in HEK293T cells ...
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bioRxiv - Cell Biology 2023Quote: ... 1 million cells were co-transfected with 1 µg ePiggyBac transposase expression plasmid (System Biosciences) and 1 µg each epB-MCP-mScarletI ...
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bioRxiv - Immunology 2019Quote: ... and used to transduce pre-activated primary human CD4+ T cells in combination with Transdux (System Biosciences). GFP+ cells were FACS sorted 48h later ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: We used the human iPSC lines mciPS (model no. SC301A-1; System Biosciences, Palo Alto, CA, USA), SBAD2 [75] ...
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bioRxiv - Developmental Biology 2022Quote: ... freshly dissociated brain cells were first transduced with lentivirus delivering human SNIP1 transgene (System Biosciences CD823A-1) in ultra-low attachment 6-well plates ...
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bioRxiv - Genetics 2019Quote: ... pB530A-puroVal2 was previously created by substituting the copepod GFP from the pB530A plasmid (System Biosciences) with a Puromycin resistance gene.
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bioRxiv - Cell Biology 2023Quote: Plasmids with expression cassettes flanked by transposable elements were co-transfected with piggyBac transposase (System Biosciences) into clone 5E6L Caco-2BBe cells ...
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bioRxiv - Cancer Biology 2023Quote: ... A second ESR1-DAB2 plasmid in a pCDH-CMV-MCS-ER1α-Puro (System Biosciences #CD510B-1) was received as a kind gift from Dr ...
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bioRxiv - Molecular Biology 2021Quote: ... human ATRX cDNA were amplified from HeLa and assembled into PB-EF1α-MCS-IRES-Neo vector (System Biosciences) by Gibson assembly ...
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bioRxiv - Neuroscience 2022Quote: ... 500 μL of human plasma were incubated with 5 μL purified thrombin (System Biosciences, Inc.; Catalog # TMEXO-1) at room temperature for 5 min ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... We created the PB transposase plasmid pSPBase by removing the ccdB gene from PB210PA-1 (System Biosciences) to render it generally propagable ...
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bioRxiv - Microbiology 2020Quote: ... hFhl1 gene encoding FHL1A isoform fused with GFP was cloned into modified PiggyBac plasmid (System Bioscience, Inc) under control of the CMV promoter ...
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bioRxiv - Genomics 2023Quote: ... A plasmid harboring a synthetic HygR-P2A-2xHA-FKBP_F36V insert was generated with Cold Fusion (System Biosciences), starting with the HygR-P2A-AID cassete in pMGS58 (Ad-dgene #135311 ...
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bioRxiv - Cancer Biology 2022Quote: ... Both human and mouse BGN were subcloned into the PB-CMV-MCS-EF1α-Puro piggybac expression vector (System Biosciences) using the 5’ EcoRI and 3’ BamHI restriction sites ...
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bioRxiv - Cancer Biology 2024Quote: cDNA of mouse Dnmt3A1WT and DnmtA1W326R (corresponding to human W330R) previously cloned into pCDH-EF1-MCS-Neo (System Biosciences) with an N-terminal FLAG-HA epitope tag(37 ...
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bioRxiv - Cell Biology 2022Quote: ... a lentivirus expression plasmid (MMIR-1a-1-PA-1 and MMIR-206-PA-1 purchased from System Biosciences) and plasmid mixture for packaging (ViraPower HiPerform Lentiviral Expression Systems ...
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bioRxiv - Genetics 2021Quote: ... along with 1/10 total plasmid mass of Super PiggyBac Transposase Expression Vector (System Bioscience, Inc. #PB210PA-1). Stable integrants were selected with 1-2 μg/mL puromycin.
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bioRxiv - Cell Biology 2021Quote: The backbone of the original plasmid pCDH-CMV-MCS-EF1-copGFP-T2A-Puro (System Biosciences, Palo Alto, USA) was amplified and linearized via PCR using the high-fidelity PrimeSTAR GXL DNA Polymerase (Takara Bio ...
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bioRxiv - Biochemistry 2023Quote: The PiggyBac-cumate-ERGIC-53-PA tag plasmid along with the Super PiggyBac Transposase Expression Vector (System Bioscience) was transfected to generate a stable HEK293T cell line with cumate inducible ERGIC-53 expression ...
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bioRxiv - Cancer Biology 2022Quote: ... Human wild-type or mutant (K4M) histone H3.1 were cloned into pCDH-EF1-MCS-IRES-RFP (System Biosciences, CD531A-2). To express sgRNAs ...
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bioRxiv - Cell Biology 2022Quote: ... The pCDH-CMV-MCS-EF1-Blast was generated from pCDH-CMV-MCS-EF1-Hygro (plasmid #CD515B-1; System Biosciences). The annealed synthetic oligonucleotides (Table 2 ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... we developed the excision-only PB transposase plasmid pXPBase-GFP through multiple cloning steps modifying PB220PA-1 (System Biosciences). The ccdB gene was removed to render the plasmid generally propagable ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmids for stable cell line construction were generated by Gibson cloning into pCDH-EF1-CymR-T2A-Neo (System Bioscience). Sequences of all plasmids were confirmed by Sanger sequencing.
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bioRxiv - Bioengineering 2023Quote: Genes-of-interest such as SB-CAR and SB100X constructs were firstly cloned into a parental plasmid (System Biosciences). Then MC DNA was produced and purified by using an MC-EsayTM Kit (System Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... was added to a tube along with purefection reagent and pPackF1 lentiviral packaging kit plasmids (System Biosciences, # LV100A-1) and either PCF1 (empty vector control) ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmids for stable cell line construction were generated by Gibson cloning into pCDH-EF1-CymR-T2A-Neo (System Bioscience) vector ...
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bioRxiv - Molecular Biology 2021Quote: The primary human T cell transductions were done with multiple viral titers using TransDux™ MAX Lentivirus Transduction Reagent (System Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: The gene for human HBEGF was synthesized and cloned into the MCS of PiggyBac vector PB-CMV-MCS-EF1α-Puro (System Biosciences) by restriction digest with EcoRI and BamHI (pCMV-hHBEGF) ...
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bioRxiv - Cancer Biology 2022Quote: ... with pPACKH1 vectors and SORE6-dsCopGFP plasmid DNA (kindly provided by Wakefield Lab, NIH) according to the manufacturer’s protocols (System Biosciences). Viral supernatant was collected at 48 hrs ...
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bioRxiv - Cancer Biology 2023Quote: ... were co-transfected with the tet-L1/GLucAI donor plasmid (pBH201) and the Super PiggyBac Transposase Expression Vector (System Biosciences) at a 5:1 plasmid ratio ...
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bioRxiv - Molecular Biology 2020Quote: MDEs were isolated from G1-G3 bovine and human milk samples (1.5 mL/sample) using the EQ reagent (System Biosciences: EXOQ5A-1) (13,14,38,40,44) ...
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bioRxiv - Molecular Biology 2019Quote: HeLa cervix carcinoma cells (American Tissue Culture Collection: Manassas, VA, USA) and human embryonic kidney (HEK) 293TN cells (System Biosciences, LV900A-1) were cultured using Dulbecco’ s Modified Eagle Medium (DMEM ...
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bioRxiv - Synthetic Biology 2020Quote: ... and then transfected with three piggyBac vectors expressing multiple human proteins with the Neon Transfection System with the presence of piggyBac Transposase (System Biosciences, USA). Two days after ...
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bioRxiv - Cell Biology 2021Quote: TYR-G1-G4 knockout cell lines were transfected with the Pb-TRE3G-TYR-HygR plasmid and piggyBac transposase (PB210PA-1, System Biosciences) in a 1:3 ratio using lipofectamine (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... TMEM106b and TfR DNA was amplified for insertion as an N-terminal fusion to mApple and subcloned into the third-generation lentiviral plasmid pCDH-EF1-IRES-Puro (System Biosciences). The plasmid encoding CMV-EGFP was generated by digesting sfGFP-TFR-20 (RRID ...
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bioRxiv - Bioengineering 2022Quote: Gene editing of COS-7 cells were performed by electroporation of COS-7 cells with Super PiggyBac plasmid (PB210PA-1, System Biosciences) and one of the 5 plasmids of pBv1-EF-6X ...
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bioRxiv - Neuroscience 2022Quote: ... HEK 293T cells cultured in 10-cm Petri dishes at a 50-60% confluence were co-transfected with 10 μg lentiviral plasmid expressing miR-124-1 precursor under the CMV promoter and GFP under the EF1 promoter (SBI System Biosciences) or 10 μg lentiviral plasmid expressing GFP under the CMV promoter and the packaging plasmids pVSV-G (3.5 μg) ...
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bioRxiv - Synthetic Biology 2023Quote: Two expression cassettes each carrying a CAG promoter driving the expression of NCas13d-GAI-T2A-BFP and GID-Cas13dC-T2A-iRFP were packed into a single PiggyBac-transposon vector plasmid (System Biosciences). HEK293FT cells were transfected with the vector and transposon plasmid (System Biosciences) ...
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bioRxiv - Genomics 2023Quote: Clones A and E were transfected with a Piggybac-PEmax plasmid with the Piggybac transposase (System Biosciences, Cat. No. PB210PA-1) at a 10:1 molar ratio using the transIT-LT1 transfection reagent (Mirius Bio ...
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bioRxiv - Molecular Biology 2023Quote: ... U2-OS cells (UC Berkeley BDS Cell Culture Facility) cultured in DMEM (1 g/L glucose, 10% FBS) were transfected with a modified PiggyBac Transposase plasmid (System Biosciences) and a PGK.mNeonGreen-LacI.NLS.IRES.Puro integration plasmid using Fugene6 transfection reagent ...