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Citations for System Biosciences :
51 - 100 of 119 citations for Genz 644282 CAS 529488 28 6 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: EV isolation from mouse serum was performed using ExoQuick™ solution (System Biosciences, Palo Alto, CA, USA). Following serum collection ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: We used the human iPSC lines mciPS (model no. SC301A-1; System Biosciences, Palo Alto, CA, USA), SBAD2 [75] ...
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bioRxiv - Neuroscience 2021Quote: ... The resulting PCR fragment was inserted by means of the cold fusion reaction (System Biosciences; CA, USA) into the EcoRI-XbaI-digested pCaSpeR-mb247-hs-GCamP3 vector (gift from T ...
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bioRxiv - Bioengineering 2023Quote: ... and anti-rabbit HRP-linked secondary antibody (EXOAB-KIT-1) were purchased from System Biosciences (CA, USA). Anti-beta-actin antibody (sc-47778) ...
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bioRxiv - Cell Biology 2024Quote: Conditioned media (n=2 each group) were sent for Exo-NGS™ services (System Biosciences, CA, USA). In brief ...
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bioRxiv - Neuroscience 2024Quote: ... supernatant were mixed with 252 μl of ExoQuick precipitation solution (EXOQ; System Biosciences, Inc., Mountain View, CA), and incubated for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... and used to infect Stat3−/− MEFs in the presence of Transdux Max (System Biosciences, Palo Alto, CA, USA) reagent ...
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bioRxiv - Cell Biology 2020Quote: Lentivirus was generated with PEG-it Virus Precipitation Solution (System Biosciences, Palo Alto, CA, USA no LV810A-1) using a LV H2B-GFP plasmid (Addgene no 25999 ...
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bioRxiv - Cancer Biology 2023Quote: ... Viral particles were harvested 2 days later and concentrated with Peg-itTM (System Biosciences, Palo Alto, CA, USA).
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bioRxiv - Microbiology 2024Quote: ... EVs were enriched from RBC culture supernatants using ExoMaxTM according to manufacturer’s instructions (System Biosciences, Palo Alto, CA). EVs were examined for protein concentration using BCA and by Western blots as described below.
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bioRxiv - Bioengineering 2024Quote: ... and the EVs were isolated with a commercially available precipitation kit (ExoQuick, System Biosciences, Palo Alto, CA, US) following the protocol provided by the vendor ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was transferred to a sterile vessel and added Exo Quick-TC (System Biosciences, Palo Alto, CA) (supernatant ...
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bioRxiv - Cancer Biology 2023Quote: ... EVs were isolated from 500 µL fresh serum using the ExoQuick protocol (System Biosciences, Palo Alto, CA, USA). Remaining serum aliquots were snap-frozen and stored at -80°C.
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bioRxiv - Cancer Biology 2024Quote: ... EVs were isolated from serum samples using ExoQuick Serum Exosome Precipitation Solution (System Biosciences, Palo Alto, CA, USA) according to manufacturers’ instructions and as before [32] ...
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bioRxiv - Molecular Biology 2020Quote: ... A forward primer of unknown sequence for XMIR-NT was initially purchased from System Bioscience (Palo Alto, CA, USA), then later custom ordered from IDT after plasmid sequencing ...
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bioRxiv - Bioengineering 2021Quote: ... Quantification of EV release was accomplished using an ExoELISA-ULTRA Complete Kit (CD63 Detection; System Biosciences, Mountain View, CA). Briefly ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: The medium was conditioned using exosome-free FBS (Exo-FBS-250A-1, System Bioscience, Palo Alto, CA, United States) and was PS-free ...
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bioRxiv - Cancer Biology 2024Quote: ... Western Blotting of EVs protein was performed using slot blot EXO-Array Exosome antibody check kit (System Biosciences, CA). Antibody slots for CD63 ...
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bioRxiv - Cancer Biology 2024Quote: ... EV RNA was isolated by EXOQUICK® Exosome isolation and RNA purification kit for serum/plasma (System Biosciences,CA) and was reverse transcribed to make amplified cDNA by SMART seq v4 ultra-low input RNA kit (Takara Bio Inc ...
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bioRxiv - Bioengineering 2020Quote: ... 100 ml of LB broth together with 100 µl of 20% arabinose induction solution (System Biosciences, Palo Alto, CA, USA) and 4 ml of 1N NaOH was added to the culture and grown for 5.5 hrs at 30°C ...
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bioRxiv - Biochemistry 2020Quote: ... The serum (50-100 μl) was treated with ExoQuick Exosome Precipitation Solution (25.2 μl/100 μl serum; System Biosciences, CA) on ice for 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... the concentrated EVs were further isolated from the remaining medium using ExoQuick-TC (SBI System Bioscience, Palo Alto, CA, USA). The isolation was performed accordingly to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... and also cloned into pCDH-MSCV-MCS-EF1α-GFP+Puro lentiviral vector (System Biosciences, Cat# CD713B-1, Palo Alto, CA) for constitutive expression ...
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bioRxiv - Cell Biology 2023Quote: The CRISPR guide RNAs were cloned into the CMV-hspCas9-H1-gRNA SmartNuclease vector (System Biosciences, Palo Alto, CA, USA). The Cas9-target sequences for PPARγ-binding sites are as follows ...
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bioRxiv - Genetics 2023Quote: The PB EGFP donor vector (PB-CMV-MCS-EF1ɑ-GFP, #PB511B-1) was purchased from System Biosciences (Palo Alto, CA). The PiggyBac transposase (PBase ...
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bioRxiv - Cell Biology 2022Quote: ... pmLF were cultivated in 175 cm2 flasks in DMEM supplemented with EV-free FBS (System Biosciences, Palo Alto, CA, USA). Importantly ...
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bioRxiv - Cell Biology 2021Quote: ... the piggyBac transposon plasmid that contains the desired transgene and a piggyBac transposase plasmid (PB210PA-1, System Biosciences, Palo Alto, CA) were electroporated into TP53-/- RPE-1 cells simultaneously using the Neon electroporation system according to the manufacturer’s instruction (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: RNA was isolated from the EVs using the Exoquick RNA column purification kit (EQ808A-1, System Biosciences, Palo Alto, CA, USA). Purified RNA was quantified using a Bioanalyzer and the Agilent RNA 6000 Pico kit (5067-1513 ...
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bioRxiv - Neuroscience 2021Quote: ... and transduced the next day with a lentivirus expressing CD63-GFP (produced from pCT-CD63-GFP, System Biosciences, Palo Alto, CA). The astrocytes expressed GFP between 48 and 72 h after transduction ...
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bioRxiv - Neuroscience 2024Quote: Total RNA isolation kits were employed to isolate RNA from cultured iMicroglia (n=4/group; System Bioscience, Palo Alto, CA, USA) and the mouse hippocampal tissues (n=5-6/group ...
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bioRxiv - Biochemistry 2020Quote: ... The secreted EVs were partially purified from cultured medium by using ExoQuick-TC Exosome Precipitation Solution (20 μl/100 μl serum; System Biosciences, CA) at 4°C for overnight ...
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bioRxiv - Immunology 2022Quote: ... VLPs were precipitated from the clarified supernatant by the addition of PEG-it Virus Precipitation Solution (System Biosciences, Palo Alto, CA, USA) at a ratio of 1:10 ...
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bioRxiv - Molecular Biology 2021Quote: ... a lentiviral Lenti/anti-miR-31 (antimiR-31) was created using commercial plasmids pLenti/anti-miR-31 (System Biosciences, Palo Alto, CA). A Lenti/anti-miR-Cont (antimiR-Cont ...
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bioRxiv - Synthetic Biology 2020Quote: ... Exo-Check Array: A549 protein markers were characterised using a commercially sourced Exo-Check Exosome Antibody Array kit (#EXORAY200A-4, System Biosciences, CA, USA). A549 EVs (100 μg ...
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bioRxiv - Microbiology 2021Quote: ... EBV-negative Akata(-) cells were treated with purified exosomes in RPMI1640 supplemented with 5% exosome-depleted FBS (System Biosciences, Palo Alto, CA, USA). After 6 h ...
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bioRxiv - Neuroscience 2020Quote: ... fibrinogen and fibrin proteins known to impede efficient EV recovery were removed using 5 U/mL of thrombin (System Biosciences, Inc.; Mountainview, CA) for 30 minutes at RT followed by addition of 495 μL of Dulbecco’s PBS-1X (DPBS ...
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bioRxiv - Physiology 2023Quote: Isolated Evs were processed for total RNA isolation using the SeraMir Exosome RNA purification kit (Cat #RA808A-1, System Biosciences, Palo Alto, CA), according to the manufacturer’s instructions provided with the kit ...
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bioRxiv - Developmental Biology 2024Quote: ... miRZip anti-miR precursor constructs for miRZip-129-5p and pGreenPuro Scramble Hairpin control constructs were purchased from System Biosciences (Palo Alto, CA), and lentiviruses were generated according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2021Quote: ... miRZip anti-miR precursor constructs for miR-322 and miR-503 cluster (miRZIP-322 and miRZip-503) and pGreenPuro Scramble Hairpin control construct were purchased from System Biosciences (Palo Alto, CA), and lentiviruses were generated according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Complete or partial DFNA5 cDNA was cloned into PiggyBac Cumate Switch Inducible vectors (PB-Cuo-MCS-IRES-GFP-EF1-CymR-T2A-Puro or PB-Cuo-MCS-3XFlag-IRES-GFP-EF1-CymR-T2A-Puro) (System Biosciences, Palo Alto, CA). The vectors were transduced into HeLa cells using the PiggyBac transposon system (System Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... MDA-MB-231 and MDA-MB-231-Luc+ cells were transduced with lentivirus vector expressing a GFP or RFP tag fused to the membrane-associated tetraspanin CD63 (System Biosciences, Palo Alto, CA). Breast cancer cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cancer Biology 2021Quote: ... The SETD7 cDNA was cloned into the EcoRI/BamHI site of a pCDH-CMV-MCS-EF1α -Puro vector (System Biosciences, Palo Alto, CA, USA). The sequences were confirmed by Sanger sequencing ...
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bioRxiv - Cell Biology 2020Quote: ... We PCR amplified the EC-BioID sequence and inserted it into the PiggyBac-GFP vector using NheI site (PB533A, System Biosciences, Mountain View, CA). For C-BioID ...
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bioRxiv - Cancer Biology 2021Quote: ... The transfer vector was then packaged into a lentivirus using the pPACKH1 HIV third generation lentiviral expression system kit and PureFection reagent (both from System Biosciences, Palo Alto, CA) into HEK293-T cells (ATCC ...
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bioRxiv - Cell Biology 2021Quote: ... we seeded cells at ∼30% confluency in exosome-depleted medium that was obtained either by ultracentrifugation of DMEM plus 10% FBS or DMEM supplemented with 10% exosome-depleted FBS (System Biosciences, Palo Alto, CA) in 150 mm CellBIND tissue culture dishes (Corning ...
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bioRxiv - Genomics 2024Quote: ... and NheI and NotI restriction sites were used to clone the insert into the pCDH-EF1-MCS-T2A-GFP lentiviral vector (System Biosciences, Palo Alto, CA). OE21 tumor cells were then transduced with the resultant lentivirus ...
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bioRxiv - Cancer Biology 2023Quote: ... omentum tissues were minced with sterile razor blades and approximately 0.5 g of minced tissue was cultured per 100 mm tissue culture dish in 10 mL DMEM/F12 media supplemented with 1% exosome-depleted FBS (EXO-FBS-250A-1, System Biosciences, Palo Alto, CA). The following day ...
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bioRxiv - Cancer Biology 2023Quote: EVs were isolated from 2.5 mL of 48 h conditioned media from tissue slice explants using ExoQuick-TC (System Biosciences, Palo Alto, CA, USA) per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: EV pellets were diluted and 45 µL was examined with the ExoELISA-ULTRA CD63 Kit according to the manufacturer’s instructions (System Biosciences, Palo Alto, CA, USA). The sample absorbance minus the blank wells was used to determine concentrations based on the standard curve included in the kit.
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bioRxiv - Cell Biology 2022Quote: ... IL) lentiviruses were grown in human embryonic kidney 293T cells and concentrated using the PEG-it Virus Precipitation Solution (System Biosciences, Mountain View, CA), as previously described (51) ...