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Citations for Spherotech :
51 - 99 of 99 citations for Streptavidin ZAP Antibody Kit goat since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... The first channel of the slide contained 4.36 μm Streptavidin coated polystyrene microspheres (Spherotech, Fulda, Germany) diluted in sample buffer (40 mM Tris ...
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bioRxiv - Biophysics 2022Quote: ... Both DNA constructs were end-biotinylated and used in combination with streptavidin-coated polystyrene beads (Spherotech) of 3.11 μm and 1.76 μm in diameter ...
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bioRxiv - Biophysics 2019Quote: ... End-biotinylated bacteriophage λ DNA was connected to streptavidin-coated polystyrene beads (Ø = 4.5 μm, Spherotech) to generate the DNA constructs ...
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bioRxiv - Biochemistry 2021Quote: ... Streptavidin-coated polystyrene particles (SVP30) with a nominal diameter of 3.11 μm were purchased from Spherotech Inc ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated with 50µL or 100µL of streptavidin magnetic particles (Spherotech, cat. SVM-40-100) / 107 cells at 2 x 107 cells/mL in FACS buffer for total DN enrichment or DN3/4 purification ...
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bioRxiv - Genetics 2022Quote: Recombinant His6-tag DASH complex was linked to 0.56 μm-diameter streptavidin-coated polystyrene microbeads (Spherotech) using biotinylated His5-antibody (Qiagen) ...
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bioRxiv - Biophysics 2023Quote: ... which were used to form DNA tethers between two 3.13-μm streptavidin-coated polystyrene beads (Spherotech) held in traps with a stiffness of ∼0.6 pN/nm ...
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bioRxiv - Biophysics 2023Quote: ... of ∼0.045 pN nm−1 while using the polystyrene bead of 0.56 μm (streptavidin conjugated, Spherotech).
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bioRxiv - Biophysics 2024Quote: ... was tethered between 4.38-µm SPHERO Streptavidin Coated polystyrene beads (Spherotech, Cat. No. SVP-40-5) in Trap1 and 2.12-µm SPHERO Anti-Dig polystyrene beads (Spherotech ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.8 μl of streptavidin-coated polystyrene beads (SA beads, 1% v/v suspension, Ø 2 μm, Spherotech) were mixed with 1 ml of assay buffer ...
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bioRxiv - Immunology 2021Quote: Biotinylated soluble TCR Cl5 (1) or scCl5 were mixed with 5-7μm streptavidin-coated UV-beads (Spherotech) in excess to ensure fully coated beads ...
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bioRxiv - Biophysics 2020Quote: ... A 10 μl reaction containing 3.5 μL of annealed complex and 1.6 μL of 0.5% streptavidin coated polystyrene beads (2 μm diameter, Spherotech) was incubated on ice for 30 min ...
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bioRxiv - Biophysics 2021Quote: ... This reaction was then diluted 5-fold into optical tweezers assay buffer containing 0.08% w/v streptavidin-coated polystyrene beads (2.1 μm diameter, Spherotech) and incubated on ice for at least 15 minutes ...
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bioRxiv - Immunology 2021Quote: Streptavidin-coated beads with different intensities of PE-channel fluorescence (Spherotech SVFA-2558-6K and SVFB-2558-6K) were incubated with the following biotinylated antigens ...
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bioRxiv - Biophysics 2021Quote: ... 5 nM 3’ overhand handles were mixed with 0.08% w/v streptavidin-coated polystyrene beads (2.1 μm diameter, Spherotech) in optical tweezers assay buffer ...
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bioRxiv - Molecular Biology 2022Quote: Two of the five channels of the microfluidic chip (Figure S5A) were correspondingly fed with a 0.01% solution of a 2.1-μm streptavidin-coated polystyrene beads (Spherotech) and a 15 pM solution of dsDNA (11.1 kbp ...
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bioRxiv - Immunology 2022Quote: A 20 μg/mL solution of PhyB protein was added to washed streptavidin-coated polystyrene beads (5.0-5.9 μm, Spherotech), before a 2h incubation on ice ...
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bioRxiv - Immunology 2023Quote: ... biotinylated flagellin peptides were conjugated on to Streptavidin coated 4um polystyrene beads (Spherotech, Inc. Cat # PAK-4067-8K). Beads conjugated with each individual peptide have different fluorescent intensity in the APC/Cy7 channel so that they can be differentiated from each other by flow cytometry ...
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bioRxiv - Biochemistry 2023Quote: ... A single reconstituted arrays or bare DNA was tethered between a pair of 3.23μm streptavidin-coated beads (Spherotech) in a buffer containing 10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2023Quote: ... Two channels of microfluidic cell were used to obtain single optically trapped DNA tether and thus were fed with a 0.01% solution of a 2.1 μm streptavidin-coated polystyrene beads (Spherotech) and 1.25 pM solution of a double stranded DNA with biotinylated ends ...
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bioRxiv - Immunology 2024Quote: Recombinant biotinylated XBB.1.5 spike protein was incubated with SPHERO Streptavidin fluorescent Yellow Particles (Spherotech, Cat. #SVF3- 2552) at 20 ng per μg beads for 30 min at room temperature on an end-over-end mixer ...
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bioRxiv - Biophysics 2019Quote: ... 2 or 2.4 µL of 0.05 nM dsDNA were incubated for an hour at room temperature with 5 µL of 0.2% w/v streptavidin-coated beads (Spherotech). Beads were diluted in approximately 300 µL of buffer (100 mM Tris ...
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bioRxiv - Biophysics 2022Quote: ... Laminar-flow-separated channels 1-3 were used to form DNA tethers between two 4.35-μm streptavidin-coated polystyrene beads (Spherotech). Channels 4 and 5 served as protein loading and imaging channels ...
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bioRxiv - Immunology 2022Quote: Streptavidin beads pre-labelled with individual intensities of PE-channel fluorophore (Spherotech, SVFA-2558-6K and SVFB-2558-6K) or FITC-channel fluorophore (Spherotech ...
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bioRxiv - Biophysics 2019Quote: ... one end of a biotinylated DNA linker (3500 base pairs) was attached to a 1.09 μm streptavidin-coated polystyrene bead (Spherotech) on the other end was attached to Halo-domain of one of the multidomain substrates ...
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bioRxiv - Cell Biology 2019Quote: ... 100 nm of WT and mutant Spc105 proteins was incubated with 10 µl of 0.1% w/v streptavidin polystyrene beads (Spherotech) conjugated with anti-penta-his biotin antibody (Qiagen ...
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bioRxiv - Biophysics 2021Quote: ... and 1 to 1.5 μL of the d-ssDNA solution was incubated for an hour at room temperature with 5 μL of 0.2% w/v streptavidin-coated microspheres (Spherotech). Beads were diluted in approximately 300 μL of buffer (100 mM Tris ...
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bioRxiv - Developmental Biology 2023Quote: ... the procedure was the same as described above despite that His-beads were substituted by Streptavidin Magnetic Particles (Spherotech).
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bioRxiv - Biophysics 2023Quote: ... 1% DMSO (Helicon) followed by addition of 10 μl streptavidin-coated microspheres (2.1 μm) (Spherotech, Cat № SVP-20-5). After washing away the unbound microspheres ...
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bioRxiv - Cancer Biology 2024Quote: Tethers were pulled from the plasma membrane using streptavidin-coated polystyrene beads with a mean diameter of 1.87 μm (SVP-15, Spherotech). Right before the experiments ...
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bioRxiv - Biophysics 2019Quote: The 8.1 kbp dsDNA construct with a primer-template junction at one terminus was tethered between 2 μm anti-digoxigenin and 3 μm streptavidin functionalized beads (Spherotech) held in place by a micropipette tip and a dual beam optical trap ...
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bioRxiv - Molecular Biology 2021Quote: ... Laminar flow separated channels 1-3 were used to form a single-stranded biotin-λ DNA tether as follows: a single 4.38-µm streptavidin-coated polystyrene bead (Spherotech) was caught in each trap in channel 1 (trap stiffness of ∼0.4 pN/nm) ...
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bioRxiv - Biophysics 2022Quote: Streptavidin-coated polystyrene nanoparticles (NPs) with a diameter of 400-700 nm (SVP-05-10; Spherotech, Lake Forest, IL, USA) were loaded with single-stranded DNA (ssDNA ...
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bioRxiv - Biophysics 2022Quote: Streptavidin-coated silica beads of 1.58 μm diameter (1.0% w/v, Spherotech, Lake forest, IL, USA, cat. SVSIP-15-5) were used for the FKBP12-rapamycin-FRB measurement ...
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bioRxiv - Immunology 2020Quote: ... recombinant SARS-CoV-2 ectodomain trimers were passively absorbed onto streptavidin functionalized fluorescent microparticles (Spherotech 3.6um cat# CPAK-3567-4K, peak 4). 500 µg of biotinylated SARS2-CoV-2 was incubated with 2E+07 Streptavidin functionalized fluorescent microparticles in 400μl of 1% BSA in PBS ...
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bioRxiv - Immunology 2024Quote: ... and XBB.1.5 strains of SARS-CoV-2 were incubated with different fluorescence intensity peaks of the SPHERO Streptavidin fluorescent Yellow Particles (Spherotech, Cat. #SVF3-2552) at 20 ng per μg beads for 30 min at room temperature on an end-over-end mixer ...
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bioRxiv - Bioengineering 2023Quote: ... The Ultra Rainbow Calibration bead kit (Spherotech URCP-38-2k) consists of 3.8 µm polystyrene particles which contain embedded six different fluorochromes that align to the most common flow cytometry excitation and emission filter sets ...
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bioRxiv - Biophysics 2022Quote: ... and a 2.12 µm polystyrene bead coated in anti-digoxigenin antibody (Spherotech cat# DIGP-20-2) was caught in trap 2 which is upstream in the path of buffer flow to trap 1 ...
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bioRxiv - Microbiology 2024Quote: ... Compensation was calculated by FACS DIVA software 6.1.3 using appropriate single antibody labeled compensation beads from SpheroTech. Data analysis was performed using FlowJo software v9.6 (Treestar).
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bioRxiv - Biophysics 2021Quote: ... 1~2 μL dilution of such streptavidin-DNA mixture containing 1~10 ng DNA was mixed with 10 μL anti-digoxigenin antibody coated polystyrene beads 2.1 μm in diameter (Spherotech) and incubated at room temperature for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... constructs were mixed with 4 μl of polystyrene beads coated with antibodies against digoxigenin (AD beads, 0.1% v/v suspension, Ø 1.76 μm, Spherotech), 10 μl of assay buffer (20 mM HEPES ...
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bioRxiv - Immunology 2020Quote: ... The antibodies and reagents for FACS analysis are listed below: SPHERO™ AccuCount Fluorescent (Spherotech, Cat.# ACFP-70-5), Anti-Human CD45 APC-Cy7 (HI30 ...
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bioRxiv - Cell Biology 2023Quote: ... an aliquot of the crosslinked protein-DNA mixture was mixed with 5 μL 2.1 μm diameter anti-digoxigenin antibody coated polystyrene beads (Spherotech) and incubated at room temperature for 15 min ...
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bioRxiv - Molecular Biology 2019Quote: ... We used the SPHERO™ Nano Fluorescent Particle Size Standard Kit, Yellow (diameters 0.22, 0.45, 0.88 and 1.35 μm) from Spherotech Inc ...
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bioRxiv - Physiology 2022Quote: ... Calibration of the cytometer was assessed using fluorescent particles of standardized size (Nano Fluorescent Particle Size Standard Kit, Spherotech).
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bioRxiv - Immunology 2023Quote: ... SA was then conjugated to spherotech 4μm and 5μm Carboxy Blue Particle Array Kits (Spherotech CPAK-4067-8K and CPAK-5067-10K) using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC ...
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bioRxiv - Immunology 2021Quote: ... and following surface staining cells were fixed and permeabilized according to the manufacturer’s instructions using the BD Cytofix/Cytoperm Plus Kit (BD. For counting the cells AccuCount Fluorecent particles (Spherotech, Lake Forest, IL, USA) were used ...
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bioRxiv - Biochemistry 2023Quote: ... as compared with standard polystyrene beads of 880 nm and 1350 nm size from the SPHEROTM Nano Fluorescent particle standard kit (Spherotech, Lake Forest, IL).37 Marker-positive platelet and microparticle count were expressed as % of all events captured in a joint gate “Platelets + Microparticles” ...
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bioRxiv - Immunology 2023Quote: Custom cytometric bead arrays were generated as previously described87 using 4 µm and 5 µm carboxy functionalized array kits (Spherotech, PAK-4067-8K and PAK-5067-10K). Streptavidin (SA ...