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Citations for Spherotech :
1 - 50 of 51 citations for PGFM ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Rainbow Calibration particles (RCP-60-5-2, RCP-60-5-5) were obtained from Spherotech. FITC and PE stained beads were part of the three color BD CaliBRITE kit (BD Biosciences 340486) ...
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bioRxiv - Immunology 2020Quote: ... we used 5 μm streptavidin-coated polystyrene bead (Spherotech #VP-60-5). We used 1,1’-carbonyldiimidazole in DMSO based system as described by Tam et al ...
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bioRxiv - Microbiology 2021Quote: ... Nile Red nominally 5 µm (5-7.9 µm) (Spherotech, FP-6056-2, 1.5 × 108/ml) and Nile Red nominally 10 µm (10-14 µm ...
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bioRxiv - Cell Biology 2021Quote: ... Fluorescent carboxylated 5 µm beads (Spherotech) were added to cells at a ratio of 5:1 and incubated for 6 h at 37°C ...
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bioRxiv - Immunology 2024Quote: ... Avidin beads (Spherotech, SVP-30-5) were pre-incubated in the same medium with different concentrations of CD4- or CD8-biotin and washed once ...
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bioRxiv - Synthetic Biology 2020Quote: ... Rainbow Calibration Particles (Spherotech #RCP-30-5) or UltraRainbow Calibration Particles (Spherotech #URCP-100-2H ...
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bioRxiv - Immunology 2022Quote: ... 7 μm streptavidin beads (Spherotech, SVP-60-5) coated in tandem with 10 μg/mL MERS-CoV spike and 10 μg/mL OC43-CoV spike were re-suspended in a cocktail of 2.5 μg/mL goat anti-human IgG-Alexa Fluor 647 (Jackson ImmunoResearch ...
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bioRxiv - Biophysics 2023Quote: ... Streptavidin-coated polystyrene beads (Spherotech, SVP-10-5) was used as reference beads for the stage drift correction ...
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bioRxiv - Immunology 2023Quote: Custom cytometric bead arrays were generated as previously described87 using 4 µm and 5 µm carboxy functionalized array kits (Spherotech, PAK-4067-8K and PAK-5067-10K). Streptavidin (SA ...
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bioRxiv - Biophysics 2021Quote: Protein G-coated polystyrene beads (Spherotech, PGP-60-5) were functionalized with PSGL1-Fc Chimera (R&D systems ...
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bioRxiv - Cell Biology 2022Quote: Rainbow Calibration Particles (RCP-05-5, lot AL01, Spherotech) with known Equivalent number of Reference Fluorophores (ERF ...
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bioRxiv - Cell Biology 2023Quote: ... BSA-Beads (for immunofluorescence, Spherotech; BP-30-5; ∼3μm) or Biotin-Beads (for lipidomics ...
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bioRxiv - Cell Biology 2023Quote: ... or Biotin-Beads (for lipidomics, Spherotech; TP-30-5; ∼3μm) served as control beads ...
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bioRxiv - Immunology 2019Quote: ... the raw data were converted to plate-calibrated ERFs using rainbow calibration particles (Spherotech). For a given cell population and readout ...
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bioRxiv - Bioengineering 2021Quote: Fluorescent PMMA (PolyAn, 5 μm pink) and PS (Spherotech, 7 μm yellow) microbeads were prepared in standard solutions ...
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bioRxiv - Biophysics 2022Quote: ... 4.2 µm polystyrene beads coated in streptavidin (Spherotech cat# SVP-40-5) were caught in each trap ...
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bioRxiv - Bioengineering 2023Quote: ... Pre-rinsed 4.5 µm streptavidin labeled polystyrene particles (Spherotech SVP-40-5) are combined with 1 µM biotinylated oligoes that are a reverse complement to the oligo covalently attached to the slide and incubated for 20 minutes at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... Biotin-coated polystyrene beads (Cat#TPX-150-5) were purchased from Spherotech.
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bioRxiv - Bioengineering 2022Quote: 5 μm in diameter polystyrene beads coated with streptavidin were purchased from Spherotech. The beads were cleaned 3 times in PBS and resuspended in a solution of PBS with 20 μg/ml biotinylated fibronectin (Cytoskeleton ...
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bioRxiv - Immunology 2023Quote: ... Channels were then flooded with 7 µm streptavidin beads (Spherotech, SVP-60-5) coated with 50 μg/mL of biotinylated RH5 and suspended in a cocktail of 2.5 μg/mL IgG-AF647 ...
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bioRxiv - Bioengineering 2023Quote: ... a known quantity of ∼5 micron AccuCount Fluorescent Beads (Spherotech, Lake Forest, IL, USA) was used for CHO MP counting.
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bioRxiv - Cell Biology 2022Quote: Streptavidin-coated polystyrene beads (3 μm in diameter, SVP-30-5, 0.5% w/v, Spherotech) were washed three times in a 10X volume of PBS and recovered by centrifugation (12,000 rpm ...
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bioRxiv - Biophysics 2022Quote: ... we additionally relied on 3 μm diameter beads (1.0% w/v, Spherotech, cat. SVSIP-30-5).
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bioRxiv - Biophysics 2024Quote: ... was tethered between 4.38-µm SPHERO Streptavidin Coated polystyrene beads (Spherotech, Cat. No. SVP-40-5) in Trap1 and 2.12-µm SPHERO Anti-Dig polystyrene beads (Spherotech ...
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bioRxiv - Biophysics 2021Quote: ... we 10-fold diluted a 5% w/v stock of polystyrene beads (PP-50-10, Spherotech, Inc.) and put 5 µm onto an easy cleaned [104] microscope glass slide ...
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bioRxiv - Immunology 2021Quote: Biotinylated soluble TCR Cl5 (1) or scCl5 were mixed with 5-7μm streptavidin-coated UV-beads (Spherotech) in excess to ensure fully coated beads ...
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bioRxiv - Biophysics 2020Quote: ... A 10 μl reaction containing 3.5 μL of annealed complex and 1.6 μL of 0.5% streptavidin coated polystyrene beads (2 μm diameter, Spherotech) was incubated on ice for 30 min ...
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bioRxiv - Biophysics 2022Quote: ... these filaments were mixed with 4 μl of 2.4 μM Neutravidin-coated beads (NVP-20-5, diameter 2.1 μm, Spherotech) and incubated for 15 minutes to couple the filaments to the beads ...
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bioRxiv - Bioengineering 2023Quote: ... soluble SiRPα-Fc at saturating concentrations were immobilized on 0.4-0.6um Protein G Yellow Fluorescent Particles (PGFP-0552-5, Spherotech) at 4°C for 1h ...
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bioRxiv - Biophysics 2019Quote: ... 2 or 2.4 µL of 0.05 nM dsDNA were incubated for an hour at room temperature with 5 µL of 0.2% w/v streptavidin-coated beads (Spherotech). Beads were diluted in approximately 300 µL of buffer (100 mM Tris ...
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bioRxiv - Immunology 2020Quote: ... The antibodies and reagents for FACS analysis are listed below: SPHERO™ AccuCount Fluorescent (Spherotech, Cat.# ACFP-70-5), Anti-Human CD45 APC-Cy7 (HI30 ...
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bioRxiv - Biophysics 2021Quote: ... and 1 to 1.5 μL of the d-ssDNA solution was incubated for an hour at room temperature with 5 μL of 0.2% w/v streptavidin-coated microspheres (Spherotech). Beads were diluted in approximately 300 μL of buffer (100 mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... 1% DMSO (Helicon) followed by addition of 10 μl streptavidin-coated microspheres (2.1 μm) (Spherotech, Cat № SVP-20-5). After washing away the unbound microspheres ...
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bioRxiv - Cell Biology 2023Quote: ... an aliquot of the crosslinked protein-DNA mixture was mixed with 5 μL 2.1 μm diameter anti-digoxigenin antibody coated polystyrene beads (Spherotech) and incubated at room temperature for 15 min ...
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bioRxiv - Synthetic Biology 2021Quote: ... to enable conversion of GFP fluorescence into calibrated MEFL units 58 a single well per plate contained 15 μL of 8-peak Rainbow Calibration Particles (Spherotech, RCP-30-5A) diluted into 200 μL PBS ...
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bioRxiv - Immunology 2021Quote: ... Cells were counted using Sphero AccuCount 5-5.9 μm beads according to the manufacturers protocol (Spherotech, Cat. #ACBP-50-10).
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bioRxiv - Biophysics 2021Quote: An optical trap (Tweez305, Aresis, Slovenia) was applied to drag an r = 1.60 μm radius polystyrene bead (HUP-30-5, Spherotech, US) in a large dextran-rich condensate at 13 different velocities (Figure 2e) ...
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bioRxiv - Biophysics 2022Quote: Streptavidin-coated silica beads of 1.58 μm diameter (1.0% w/v, Spherotech, Lake forest, IL, USA, cat. SVSIP-15-5) were used for the FKBP12-rapamycin-FRB measurement ...
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bioRxiv - Immunology 2023Quote: ... Cells were counted using Sphero AccuCount 5-5.9 μm beads according to the manufacturer’s protocol (Spherotech, Cat. #ACBP-50-10).
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bioRxiv - Microbiology 2024Quote: ... No. PP-10-10, 5% w/v, 1.23 µm mean diameter, ∼4.89 × 1010 particles/mL, obtained from Spherotech, Illinois USA), beads were diluted to 108 particles/mL in PBS prior to the addition of virus ...
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bioRxiv - Biophysics 2021Quote: ... Anti-digoxigenin antibodies were coupled to 1 um polystyrene beads via NHS-EDC coupling: 50 ul of 5% (w/v) 1 um carboxylated polystyrene beads (Spherotech, USA) was buffer exchanged in to reaction buffer (MES-NaOH ...
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bioRxiv - Bioengineering 2023Quote: ... The Ultra Rainbow Calibration bead kit (Spherotech URCP-38-2k) consists of 3.8 µm polystyrene particles which contain embedded six different fluorochromes that align to the most common flow cytometry excitation and emission filter sets ...
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bioRxiv - Microbiology 2023Quote: ... cells were counted by flow cytometry using spike-in of a known amount of AccuCount Blank Particles 5-5.9 µm (Spherotech, ACBP-50-10) as previously described7.
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bioRxiv - Cell Biology 2020Quote: The molecules of equivalent soluble fluorochrome (MESF) were calculated for PE and CFSE using SPERO TM Rainbow Beads Calibration Particles (Spherotech RCP-05-5, USA) according to instructions provided by the manufacturer ...
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bioRxiv - Molecular Biology 2019Quote: ... We used the SPHERO™ Nano Fluorescent Particle Size Standard Kit, Yellow (diameters 0.22, 0.45, 0.88 and 1.35 μm) from Spherotech Inc ...
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bioRxiv - Immunology 2023Quote: ... recombinant proteins were passively absorbed onto streptavidin functionalized 4-µm fluorescent microparticles (Carboxy Blue Particle Array Kit, Spherotech). 500 µg of biotinylated recombinant protein was incubated with 2 x 107 streptavidin functionalized fluorescent microparticles in 400 µL of 1% BSA in PBS ...
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bioRxiv - Physiology 2022Quote: ... Calibration of the cytometer was assessed using fluorescent particles of standardized size (Nano Fluorescent Particle Size Standard Kit, Spherotech).
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bioRxiv - Immunology 2022Quote: ... and BA.1 strains of SARS-CoV-2 and biotinylated BSA were incubated for 30 min on ice with different fluorescence intensity peaks of the Streptavidin Coated Fluorescent Yellow Particle Kit (Spherotech) at 9.12 ng per μg beads ...
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bioRxiv - Immunology 2023Quote: ... SA was then conjugated to spherotech 4μm and 5μm Carboxy Blue Particle Array Kits (Spherotech CPAK-4067-8K and CPAK-5067-10K) using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC ...
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bioRxiv - Immunology 2021Quote: ... and following surface staining cells were fixed and permeabilized according to the manufacturer’s instructions using the BD Cytofix/Cytoperm Plus Kit (BD. For counting the cells AccuCount Fluorecent particles (Spherotech, Lake Forest, IL, USA) were used ...