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Citations for Spherotech :
1 - 50 of 65 citations for Mouse Two pore calcium channel protein 2 Tpcn2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... Laminar-flow-separated channels 1-3 were used to form DNA tethers between two 4.35-μm streptavidin-coated polystyrene beads (Spherotech). Channels 4 and 5 served as protein loading and imaging channels ...
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bioRxiv - Immunology 2022Quote: ... or FITC-channel fluorophore (Spherotech, SVFA-2552-6K and SVFA-2552-6K) were incubated with 10 μg/mL each of a subset or all of the following biotinylated antigens ...
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bioRxiv - Biophysics 2023Quote: ... Measurements were performed using a dumbbell conformation: the protein of interest was attached in between two 1 μm-sized silica beads (Spherotech, US), using double stranded (ds ...
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bioRxiv - Immunology 2023Quote: ... Channels were then flooded with 7 µm streptavidin beads (Spherotech, SVP-60-5) coated with 50 μg/mL of biotinylated RH5 and suspended in a cocktail of 2.5 μg/mL IgG-AF647 ...
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bioRxiv - Biophysics 2022Quote: ... we trapped two streptavidin-coated polystyrene beads (Spherotech) with a 1064-nm trapping laser and moved these beads to a channel containing biotinylated λDNA (LUMICKS) ...
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bioRxiv - Biophysics 2021Quote: ... The first channel of the slide contained 4.36 μm Streptavidin coated polystyrene microspheres (Spherotech, Fulda, Germany) diluted in sample buffer (40 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... the fluorescence intensity for each channel was calibrated with beads having known amounts of fluorophore attached (Spherotech, Inc.). Finally ...
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bioRxiv - Synthetic Biology 2019Quote: ... fluorescence channel measurements were converted to units of MEFL using 8-peak rainbow beads (Spherotech RCP-30-5A) and using linear color translation and compensation between fluorophores ...
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bioRxiv - Immunology 2021Quote: Streptavidin-coated beads with different intensities of PE-channel fluorescence (Spherotech SVFA-2558-6K and SVFB-2558-6K) were incubated with the following biotinylated antigens ...
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bioRxiv - Immunology 2022Quote: Streptavidin beads pre-labelled with individual intensities of PE-channel fluorophore (Spherotech, SVFA-2558-6K and SVFB-2558-6K) or FITC-channel fluorophore (Spherotech ...
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bioRxiv - Immunology 2023Quote: In-channel IgG capture assays were performed using single assay mixtures containing anti-human IgG-coated 6-8μm beads (Spherotech) and fluorescently-labeled detection anti-IgG antibodies (2.5μg/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... using two polystyrene microspheres coated with streptavidin (Spherotech, 2.1 μm diameter) and held by two optical traps according to the method presented in [15,16] ...
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bioRxiv - Molecular Biology 2021Quote: ... Gain of the various channels was optimized by analyzing 8 Peak Rainbow Calibration Beads from Spherotech (Cat. RCP-30-5A). Gain was increased incrementally from 25 to 3000 in each channel ...
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bioRxiv - Biophysics 2021Quote: ... DNA molecules were captured between two trapped streptavidin-coated polystyrene beads ( ?4.38 µm, Spherotech).
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bioRxiv - Biophysics 2023Quote: ... Individual DNA molecules were trapped between two 1.76 μm diameter streptavidin-coated polystyrene beads (Spherotech) initially injected into a separate channel.
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bioRxiv - Immunology 2023Quote: ... recombinant proteins were passively absorbed onto streptavidin functionalized 4-µm fluorescent microparticles (Carboxy Blue Particle Array Kit, Spherotech). 500 µg of biotinylated recombinant protein was incubated with 2 x 107 streptavidin functionalized fluorescent microparticles in 400 µL of 1% BSA in PBS ...
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bioRxiv - Biophysics 2023Quote: ... which were used to form DNA tethers between two 3.13-μm streptavidin-coated polystyrene beads (Spherotech) held in traps with a stiffness of ∼0.6 pN/nm ...
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bioRxiv - Cancer Biology 2022Quote: ... and PMT voltages were adjusted to predetermined target channels using the seventh peak of 8-peak Rainbow Calibration Particles (Spherotech, Lake Forest, IL, Cat. No. RCP-30-5A;) as a reference point ...
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bioRxiv - Immunology 2020Quote: ... antibodies in PBS were mixed with 5E+07 fluorescent microparticles each with a unique fluorescent intensity in the far red channels (Spherotech 3.6um cat# CPAK-3567-4K, peaks 1-3) resuspended in 0.1 M MES buffer pH 5.0 ...
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bioRxiv - Neuroscience 2019Quote: ... fluorescent microspheres (Spherotech, FP4060-2) were imaged following the same procedures used for biocytin filled cells (Figure S5).
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bioRxiv - Cell Biology 2023Quote: ... Streptavidin-coated beads (2 μm diameter, Spherotech) were introduced into the chamber and incubated for 3 minutes to allow the beads to settle on the biotinylated coverslip surface ...
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bioRxiv - Biophysics 2021Quote: Protein G-coated polystyrene beads (Spherotech, PGP-60-5) were functionalized with PSGL1-Fc Chimera (R&D systems ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µm and 3.4 µm polystyrene beads (Spherotech) were used for size calibration ...
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bioRxiv - Neuroscience 2020Quote: ... Biotinylated proteins were purified with Neutradivin coated magnetic beads (Spherotech) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... which were purchased from Spherotech (Cat. No. FCM-4056-2).
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bioRxiv - Biophysics 2021Quote: ... 1% w/v 800 nm Protein G-coated polystyrene beads (Spherotech Inc) were washed in the same way as the streptavidin beads ...
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bioRxiv - Microbiology 2022Quote: Purified biotinylated proteins were multimerised on streptavidin coated Nile Red fluorescent beads (Spherotech Inc ...
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bioRxiv - Microbiology 2021Quote: To measure shedding of polystyrene beads (Spherotech FP-0552-2, sky blue), flow cytometry was used to quantify the number of egested beads ...
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bioRxiv - Neuroscience 2022Quote: ... of 500 nm diameter fluorescent polystyrene beads (Spherotech, Inc., FICP-08-2) embedded in agarose placed inside a quartz cuvette (Thorlabs ...
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bioRxiv - Neuroscience 2021Quote: ... voltages were set according to optimal PMT sensitivity using the peak 2 (Spherotech) voltration technique described previously by Maeker and Trotter 35 ...
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bioRxiv - Neuroscience 2024Quote: ... voltages were setup according to optimal PMT sensitivity using the peak 2 (Spherotech) voltration technique described previously by Maeker and Trotter64 ...
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bioRxiv - Immunology 2021Quote: ... serum and IL-2 starved OT1 CTLs were incubated with streptavidin polystyrene beads (Spherotech) coated with H-2Kb-OVA and ICAM-1 at a 1:1 ratio for various times at 37 ºC and immediately lysed in 2× cold lysis buffer containing phosphatase inhibitors (1 mM NaF and 0.1 mM Na3VO4 ...
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bioRxiv - Neuroscience 2022Quote: ... Tethers were pulled with a 4 µm diameter polystyrene bead (Spherotech #DIGP-40-2) held at the tip of a micropipette and controlled by micromanipulators ...
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bioRxiv - Neuroscience 2021Quote: ... voltages were set up according to optimal PMT sensitivity using the peak 2 (Spherotech) voltration technique described previously by Maeker and Trotter [46] ...
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bioRxiv - Neuroscience 2023Quote: ... Voltages were set up according to optimal PMT sensitive using the peak 2 (Spherotech) voltration technique ...
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bioRxiv - Microbiology 2021Quote: ... Nile Red nominally 5 µm (5-7.9 µm) (Spherotech, FP-6056-2, 1.5 × 108/ml) and Nile Red nominally 10 µm (10-14 µm ...
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bioRxiv - Immunology 2020Quote: ... Routinely alignments were performed with Ultra Rainbow Fluorescent Particles 3.0 µm (Spherotech, URFP-30-2). The triggering threshold was applied to the side scatter on the 488-SSC channel and the relative applied voltage was determined by using size reference beads (Apogee flow systems ...
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bioRxiv - Cell Biology 2023Quote: ... We then incubated each sample with 0.22-μm light yellow beads (Spherotech, FP-0245-2) resuspended in 2× SSC as fiducial markers for drift correction and washed the sample with 2× SSC briefly.
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bioRxiv - Bioengineering 2023Quote: ... Rainbow Calibration particles (RCP-60-5-2, RCP-60-5-5) were obtained from Spherotech. FITC and PE stained beads were part of the three color BD CaliBRITE kit (BD Biosciences 340486) ...
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bioRxiv - Immunology 2022Quote: A 20 μg/mL solution of PhyB protein was added to washed streptavidin-coated polystyrene beads (5.0-5.9 μm, Spherotech), before a 2h incubation on ice ...
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bioRxiv - Bioengineering 2023Quote: ... soluble SiRPα-Fc at saturating concentrations were immobilized on 0.4-0.6um Protein G Yellow Fluorescent Particles (PGFP-0552-5, Spherotech) at 4°C for 1h ...
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bioRxiv - Immunology 2024Quote: Recombinant biotinylated XBB.1.5 spike protein was incubated with SPHERO Streptavidin fluorescent Yellow Particles (Spherotech, Cat. #SVF3- 2552) at 20 ng per μg beads for 30 min at room temperature on an end-over-end mixer ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 2 µl of 1% w/v amine polystyrene fluorescent yellow particles (NH2-beads, Spherotech, Inc) or carboxyl polystyrene fluorescent yellow particles (Spherotech ...
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bioRxiv - Biophysics 2022Quote: ... and a 2.12 µm polystyrene bead coated in anti-digoxigenin antibody (Spherotech cat# DIGP-20-2) was caught in trap 2 which is upstream in the path of buffer flow to trap 1 ...
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bioRxiv - Microbiology 2021Quote: ... and Nile Red nominally 10 µm (10-14 µm) (Spherotech, FH-10056-2, 107 microspheres/ml) with unmodified surfaces ...
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bioRxiv - Biophysics 2024Quote: ... in Trap1 and 2.12-µm SPHERO Anti-Dig polystyrene beads (Spherotech, Cat. No. DIGP-20-2) in Trap2 ...
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bioRxiv - Cell Biology 2019Quote: ... 100 nm of WT and mutant Spc105 proteins was incubated with 10 µl of 0.1% w/v streptavidin polystyrene beads (Spherotech) conjugated with anti-penta-his biotin antibody (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... an aliquot of the crosslinked protein-DNA mixture was mixed with 5 μL 2.1 μm diameter anti-digoxigenin antibody coated polystyrene beads (Spherotech) and incubated at room temperature for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.8 μl of streptavidin-coated polystyrene beads (SA beads, 1% v/v suspension, Ø 2 μm, Spherotech) were mixed with 1 ml of assay buffer ...
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bioRxiv - Biophysics 2022Quote: An aliquot of the crosslinked protein-DNA sample was incubated with 1 μL anti-digoxigenin coated polystyrene beads 2.17 μm in diameter (Spherotech, IL), diluted in 1 mL phosphate-buffered saline (PBS) ...