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Citations for Spherotech :
51 - 73 of 73 citations for IL 5 Rat CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: 5 μm in diameter polystyrene beads coated with streptavidin were purchased from Spherotech. The beads were cleaned 3 times in PBS and resuspended in a solution of PBS with 20 μg/ml biotinylated fibronectin (Cytoskeleton ...
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bioRxiv - Immunology 2023Quote: ... Channels were then flooded with 7 µm streptavidin beads (Spherotech, SVP-60-5) coated with 50 μg/mL of biotinylated RH5 and suspended in a cocktail of 2.5 μg/mL IgG-AF647 ...
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bioRxiv - Cell Biology 2022Quote: Streptavidin-coated polystyrene beads (3 μm in diameter, SVP-30-5, 0.5% w/v, Spherotech) were washed three times in a 10X volume of PBS and recovered by centrifugation (12,000 rpm ...
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bioRxiv - Biophysics 2022Quote: ... we additionally relied on 3 μm diameter beads (1.0% w/v, Spherotech, cat. SVSIP-30-5).
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bioRxiv - Biophysics 2024Quote: ... was tethered between 4.38-µm SPHERO Streptavidin Coated polystyrene beads (Spherotech, Cat. No. SVP-40-5) in Trap1 and 2.12-µm SPHERO Anti-Dig polystyrene beads (Spherotech ...
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bioRxiv - Biophysics 2021Quote: ... we 10-fold diluted a 5% w/v stock of polystyrene beads (PP-50-10, Spherotech, Inc.) and put 5 µm onto an easy cleaned [104] microscope glass slide ...
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bioRxiv - Immunology 2021Quote: Biotinylated soluble TCR Cl5 (1) or scCl5 were mixed with 5-7μm streptavidin-coated UV-beads (Spherotech) in excess to ensure fully coated beads ...
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bioRxiv - Biophysics 2020Quote: ... A 10 μl reaction containing 3.5 μL of annealed complex and 1.6 μL of 0.5% streptavidin coated polystyrene beads (2 μm diameter, Spherotech) was incubated on ice for 30 min ...
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bioRxiv - Biophysics 2022Quote: ... these filaments were mixed with 4 μl of 2.4 μM Neutravidin-coated beads (NVP-20-5, diameter 2.1 μm, Spherotech) and incubated for 15 minutes to couple the filaments to the beads ...
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bioRxiv - Bioengineering 2023Quote: ... soluble SiRPα-Fc at saturating concentrations were immobilized on 0.4-0.6um Protein G Yellow Fluorescent Particles (PGFP-0552-5, Spherotech) at 4°C for 1h ...
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bioRxiv - Biophysics 2019Quote: ... 2 or 2.4 µL of 0.05 nM dsDNA were incubated for an hour at room temperature with 5 µL of 0.2% w/v streptavidin-coated beads (Spherotech). Beads were diluted in approximately 300 µL of buffer (100 mM Tris ...
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bioRxiv - Immunology 2020Quote: ... The antibodies and reagents for FACS analysis are listed below: SPHERO™ AccuCount Fluorescent (Spherotech, Cat.# ACFP-70-5), Anti-Human CD45 APC-Cy7 (HI30 ...
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bioRxiv - Biophysics 2021Quote: ... and 1 to 1.5 μL of the d-ssDNA solution was incubated for an hour at room temperature with 5 μL of 0.2% w/v streptavidin-coated microspheres (Spherotech). Beads were diluted in approximately 300 μL of buffer (100 mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... 1% DMSO (Helicon) followed by addition of 10 μl streptavidin-coated microspheres (2.1 μm) (Spherotech, Cat № SVP-20-5). After washing away the unbound microspheres ...
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bioRxiv - Cell Biology 2023Quote: ... an aliquot of the crosslinked protein-DNA mixture was mixed with 5 μL 2.1 μm diameter anti-digoxigenin antibody coated polystyrene beads (Spherotech) and incubated at room temperature for 15 min ...
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bioRxiv - Immunology 2021Quote: ... Cells were counted using Sphero AccuCount 5-5.9 μm beads according to the manufacturers protocol (Spherotech, Cat. #ACBP-50-10).
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bioRxiv - Biophysics 2021Quote: An optical trap (Tweez305, Aresis, Slovenia) was applied to drag an r = 1.60 μm radius polystyrene bead (HUP-30-5, Spherotech, US) in a large dextran-rich condensate at 13 different velocities (Figure 2e) ...
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bioRxiv - Immunology 2023Quote: ... Cells were counted using Sphero AccuCount 5-5.9 μm beads according to the manufacturer’s protocol (Spherotech, Cat. #ACBP-50-10).
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bioRxiv - Microbiology 2024Quote: ... No. PP-10-10, 5% w/v, 1.23 µm mean diameter, ∼4.89 × 1010 particles/mL, obtained from Spherotech, Illinois USA), beads were diluted to 108 particles/mL in PBS prior to the addition of virus ...
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bioRxiv - Biophysics 2021Quote: ... Anti-digoxigenin antibodies were coupled to 1 um polystyrene beads via NHS-EDC coupling: 50 ul of 5% (w/v) 1 um carboxylated polystyrene beads (Spherotech, USA) was buffer exchanged in to reaction buffer (MES-NaOH ...
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bioRxiv - Microbiology 2023Quote: ... cells were counted by flow cytometry using spike-in of a known amount of AccuCount Blank Particles 5-5.9 µm (Spherotech, ACBP-50-10) as previously described7.
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bioRxiv - Cell Biology 2020Quote: The molecules of equivalent soluble fluorochrome (MESF) were calculated for PE and CFSE using SPERO TM Rainbow Beads Calibration Particles (Spherotech RCP-05-5, USA) according to instructions provided by the manufacturer ...
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bioRxiv - Immunology 2023Quote: Custom cytometric bead arrays were generated as previously described87 using 4 µm and 5 µm carboxy functionalized array kits (Spherotech, PAK-4067-8K and PAK-5067-10K). Streptavidin (SA ...