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Citations for Spherotech :
1 - 50 of 58 citations for IL 1 beta Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... APC counting beads (Spherotech, IL) were added ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... counting beads (Spherotech; Lake Forest, IL), BSA (Rockland Immunochemicals ...
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bioRxiv - Immunology 2019Quote: ... 8-peak (Spherotech Inc.; Lake Forest, IL). Compensation was calculated using FACS DIVATM Software (BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... SPHERO AccuCount particles (Spherotech, Lake Forest, IL) were added to samples immediately before analysis to calculate absolute number of each cell population.
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bioRxiv - Immunology 2021Quote: ... Counting beads (10 µm, Spherotech, Lake Forest, IL) were added at 5000 per sample ...
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bioRxiv - Biophysics 2021Quote: ... streptavidin-coated polystyrene beads (Spherotech, Lake forest, IL, USA), while the beads used in MT measurements were 1.0 μm diameter ...
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bioRxiv - Genomics 2020Quote: ... silica coated superparamagnetic beads from Spherotech (Lake Forest, IL), 1M Tris pH 8.0 and 0.5 M EDTA from Quality Biologicals (Gaithersburg ...
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bioRxiv - Cell Biology 2019Quote: ... SPHERO Drop Delay Calibration Particles (Spherotech, Lake Forest, IL) were used to confirm the drop targeting ...
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bioRxiv - Biophysics 2022Quote: An aliquot of the crosslinked protein-DNA sample was incubated with 1 μL anti-digoxigenin coated polystyrene beads 2.17 μm in diameter (Spherotech, IL), diluted in 1 mL phosphate-buffered saline (PBS) ...
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bioRxiv - Immunology 2020Quote: ... we used Rainbow beads (Spherotech, Inc, Lake Forest, IL, USA). For every run ...
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bioRxiv - Biophysics 2024Quote: ... Streptavidin coated 440 nm polystyrene beads (Spherotech, Lake Forest IL) were functionalized with biotinylated α-penta-His antibody (Qiagen ...
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bioRxiv - Bioengineering 2023Quote: ... 106/mL AccuCount Fluorescent Beads (Spherotech, Lake Forest, IL, USA) was added to each sample for MkEV quantification ...
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bioRxiv - Bioengineering 2021Quote: The fluorescent yellow microbeads (10.2 μm; Spherotech, Lake Forest, IL, USA) were incubated with Pierce protein-free blocking buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... streptavidin-coated polystyrene beads (0.56 μm in diameter, Spherotech Inc., Libertyville IL) were functionalized with biotinylated anti-5His antibodies (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... The 8-peak and 6-peak validation bead suspensions (Spherotech, IL, USA) were used as internal references ...
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bioRxiv - Biophysics 2022Quote: Streptavidin-coated silica beads (diameter 1.56 μm) were purchased from Spherotech (Lake Forest, IL). All other reagents were purchased from Sigma-Aldrich (St ...
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bioRxiv - Immunology 2021Quote: ... serum and IL-2 starved OT1 CTLs were incubated with streptavidin polystyrene beads (Spherotech) coated with H-2Kb-OVA and ICAM-1 at a 1:1 ratio for various times at 37 ºC and immediately lysed in 2× cold lysis buffer containing phosphatase inhibitors (1 mM NaF and 0.1 mM Na3VO4 ...
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bioRxiv - Bioengineering 2020Quote: ... Streptavidin-coated magnetic particles (3.0-3.9 μm) were obtained from Spherotech (Lake Forest, IL). For capturing EVs ...
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bioRxiv - Bioengineering 2023Quote: ... with size limits determined using fluorescent calibration beads from Spherotech (Lake Forest, IL, USA). For surface protein examination ...
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bioRxiv - Bioengineering 2023Quote: ... a known quantity of ∼5 micron AccuCount Fluorescent Beads (Spherotech, Lake Forest, IL, USA) was used for CHO MP counting.
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bioRxiv - Bioengineering 2023Quote: ... size gates were constructed using fluorescent calibration beads from Spherotech (Lake Forest, IL, USA). For experiments using the BD FACSAriaII flow cytometer ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei counts were determined using an internal control bead population (Spherotech, Lake Forest, IL, USA) at 106 beads/mL ...
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bioRxiv - Biophysics 2021Quote: ... The samples were tethered to a 0.8-μm streptavidin-coated polystyrene bead (Spherotech, Lake Forest, IL) at one end through biotin-streptavidin interactions and to the anti-digoxigenin (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... Calibration of flow cytometer was performed with the three-millimeter rainbow calibration particles (Spherotech, Libertyville, IL) before each run ...
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bioRxiv - Cell Biology 2023Quote: Fluorescent beads experiments: 10–14 µm polystyrene beads (SPHERO™, Spherotech, Inc., Lake Forest, IL, USA) were embedded in 20 µl fibrin gels (10µl thrombin ...
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bioRxiv - Cell Biology 2024Quote: Glutathione-functionalized beads with a diameter of 2 µm (Spherotech, GSHP-20-5, Lake Forest, IL) were sonicated for 10 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Total number of cells in tumors and spleens were determined using SPHERO AccuCount Fluorescent Particles (Spherotech, IL). Expression of H-2Kb and PD-L1 were assayed in the CD45− fraction generated from WT and CCN4-KO YUMM1.7 tumors using the antibodies ...
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bioRxiv - Microbiology 2022Quote: ... using a known amount of spike-in AccuCount Blank particles (Spherotech, Lake Forest, IL, Cat. #ACBP-50-10). Data analysis was performed using FlowJo v10.8.1 software (BD Biosciences) ...
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bioRxiv - Cell Biology 2019Quote: ... was used to capture flow of Sphero™ 2 μm high intensity fluorescent beads (Spherotech, Lake Forest, IL, USA). For association studies ...
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bioRxiv - Biophysics 2022Quote: Streptavidin-coated polystyrene nanoparticles (NPs) with a diameter of 400-700 nm (SVP-05-10; Spherotech, Lake Forest, IL, USA) were loaded with single-stranded DNA (ssDNA ...
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bioRxiv - Biophysics 2022Quote: Streptavidin-coated silica beads of 1.58 μm diameter (1.0% w/v, Spherotech, Lake forest, IL, USA, cat. SVSIP-15-5) were used for the FKBP12-rapamycin-FRB measurement ...
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bioRxiv - Neuroscience 2020Quote: ... The polystyrene beads (2.1 μm in diameter) with anti-digoxigenin coating used in optical tweezer experiments were purchased from Spherotech (Green Oaks, IL).
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bioRxiv - Immunology 2021Quote: ... and following surface staining cells were fixed and permeabilized according to the manufacturer’s instructions using the BD Cytofix/Cytoperm Plus Kit (BD. For counting the cells AccuCount Fluorecent particles (Spherotech, Lake Forest, IL, USA) were used ...
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bioRxiv - Biochemistry 2023Quote: ... as compared with standard polystyrene beads of 880 nm and 1350 nm size from the SPHEROTM Nano Fluorescent particle standard kit (Spherotech, Lake Forest, IL).37 Marker-positive platelet and microparticle count were expressed as % of all events captured in a joint gate “Platelets + Microparticles” ...
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bioRxiv - Cancer Biology 2022Quote: ... and PMT voltages were adjusted to predetermined target channels using the seventh peak of 8-peak Rainbow Calibration Particles (Spherotech, Lake Forest, IL, Cat. No. RCP-30-5A;) as a reference point ...
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bioRxiv - Immunology 2023Quote: In-channel IgG capture assays were performed using single assay mixtures containing anti-human IgG-coated 6-8μm beads (Spherotech) and fluorescently-labeled detection anti-IgG antibodies (2.5μg/mL ...
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bioRxiv - Immunology 2023Quote: ... 1 x 104 counting beads (Spherotech) were added to stained samples at the final step ...
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bioRxiv - Biophysics 2021Quote: ... Anti-digoxigenin antibodies were coupled to 1 um polystyrene beads via NHS-EDC coupling: 50 ul of 5% (w/v) 1 um carboxylated polystyrene beads (Spherotech, USA) was buffer exchanged in to reaction buffer (MES-NaOH ...
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bioRxiv - Microbiology 2024Quote: ... Channel 1 contained 4.38 µm streptavidin-coated polystyrene beads (Spherotech). Channel 2 contained the DNA substrate harboring 3x parS sequences or the control DNA without parS both of them labeled with multiple biotins at both ends ...
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bioRxiv - Biophysics 2021Quote: ... 1% w/v 800 nm Protein G-coated polystyrene beads (Spherotech Inc) were washed in the same way as the streptavidin beads ...
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bioRxiv - Biochemistry 2019Quote: ... prior to binding of the complex to 1 μM diameter polystyrene beads (Spherotech) functionalized with an anti-digoxigenin antibody (Roche ...
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bioRxiv - Biophysics 2021Quote: ... 50 μl of 1% w/v 800-nm streptavidin-coated polystyrene beads (Spherotech Inc) were washed three times in 200 μl 1x PBS buffer and centrifuged at 7500 rpm ...
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bioRxiv - Neuroscience 2020Quote: ... microglia was incubated with ANXV (1:20) for 15’ and fluorescent particles (3um) (Spherotech) were added to microglial cells at a 1:1 microglia/beads ratio for 2hr ...
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bioRxiv - Immunology 2021Quote: ... Frequency calculation was obtained by mixing 1/10th of sample with 200,000 fluorescent beads (Spherotech) for normalization (16) ...
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bioRxiv - Microbiology 2020Quote: ... Each cytometer run was calibrated with 1 mm diameter fluorescent Ultra Rainbow beads (Spherotech Inc.). 100 μL for autofluorescent cells and 75 μL for heterotrophic cells were run at an average flow rate of 20 μL min−1 and monitored with a liquid flowmeter (Sensirion US) ...
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bioRxiv - Immunology 2020Quote: ... Frequency calculation was obtained by mixing 1/10th of sample with 200,000 fluorescent beads (Spherotech) for normalization (Su et al. ...
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bioRxiv - Immunology 2024Quote: ... Frequency calculation was obtained by mixing 1/10th of samples with 200,000 fluorescent beads (Spherotech) for normalization.
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bioRxiv - Synthetic Biology 2021Quote: ... and 2 µl of 1% w/v amine polystyrene fluorescent yellow particles (NH2-beads, Spherotech, Inc) or carboxyl polystyrene fluorescent yellow particles (Spherotech ...
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bioRxiv - Biophysics 2023Quote: ... of ∼0.045 pN nm−1 while using the polystyrene bead of 0.56 μm (streptavidin conjugated, Spherotech).
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bioRxiv - Molecular Biology 2021Quote: ... 0.8 μl of streptavidin-coated polystyrene beads (SA beads, 1% v/v suspension, Ø 2 μm, Spherotech) were mixed with 1 ml of assay buffer ...