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Citations for Spherotech :
51 - 89 of 89 citations for Glucose Fluorescent Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2019Quote: ... Laser alignment was checked using 3 µm Ultra Rainbow fluorescent particles (Spherotech CAT# RCP-30-5A). Cytometer settings were maintained between WT and ex-conjugate P ...
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bioRxiv - Cell Biology 2023Quote: Fluorescent beads experiments: 10–14 µm polystyrene beads (SPHERO™, Spherotech, Inc., Lake Forest, IL, USA) were embedded in 20 µl fibrin gels (10µl thrombin ...
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bioRxiv - Cancer Biology 2021Quote: ... Total number of cells in tumors and spleens were determined using SPHERO AccuCount Fluorescent Particles (Spherotech, IL). Expression of H-2Kb and PD-L1 were assayed in the CD45− fraction generated from WT and CCN4-KO YUMM1.7 tumors using the antibodies ...
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bioRxiv - Bioengineering 2021Quote: ... One percent (w/v) stock solutions of 1-1.4μm high-intensity polystyrene Nile red fluorescent microparticles were procured from Spherotech Inc (FH-1056-2 ...
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bioRxiv - Bioengineering 2023Quote: ... soluble SiRPα-Fc at saturating concentrations were immobilized on 0.4-0.6um Protein G Yellow Fluorescent Particles (PGFP-0552-5, Spherotech) at 4°C for 1h ...
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bioRxiv - Immunology 2024Quote: Recombinant biotinylated XBB.1.5 spike protein was incubated with SPHERO Streptavidin fluorescent Yellow Particles (Spherotech, Cat. #SVF3- 2552) at 20 ng per μg beads for 30 min at room temperature on an end-over-end mixer ...
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bioRxiv - Immunology 2022Quote: ... cells from triplicate staining wells were resuspended and pooled using 100µL FACS buffer and then combined with 7500 AccuCount Ultra Rainbow Fluorescent counting beads (Spherotech; hereafter referred to as counting beads ...
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bioRxiv - Immunology 2020Quote: ... The antibodies and reagents for FACS analysis are listed below: SPHERO™ AccuCount Fluorescent (Spherotech, Cat.# ACFP-70-5), Anti-Human CD45 APC-Cy7 (HI30 ...
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bioRxiv - Immunology 2021Quote: ... Samples were acquired with optimal photomultiplier tube voltages calibrated daily using targets for SPHERO Rainbow Fluorescent Particles (Spherotech, Inc.).
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bioRxiv - Synthetic Biology 2020Quote: ... Fluorescence data was calibrated to molecules of equivalent fluorophore (MEF) using fluorescent calibration beads (Spherotech, part no. RCP-30-20A). The cytometer was programmed to measure a 25 μL portion of each cell sample ...
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bioRxiv - Biophysics 2020Quote: ... Fluorescence data was calibrated to molecules of equivalent fluorophore (MEF) using fluorescent calibration beads (Spherotech, part no. RCP-30-20A). The cytometer was programmed to measure a 25 µL portion of each cell sample ...
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bioRxiv - Plant Biology 2019Quote: ... Nanophytoplankton were distinguished from picoplankton based on light scatter (FSC and SSC) and pulse width (transit time) compared to 3 µm UltraRainbow fluorescent particles (Spherotech).
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bioRxiv - Molecular Biology 2022Quote: ... Arbitrary fluorescent values from the flow cytometer were converted to molecules of equivalent fluorescein (MEFL) by measuring the fluorescence from Spherotech rainbow 8-peak calibration beads (Cat# RCP305A) ...
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bioRxiv - Synthetic Biology 2020Quote: ... GFPmut3* fluorescence units were converted into standardized units of molecules of equivalent fluorescein (MEFL) using a fluorescent bead standard (Rainbow calibration standard; cat. no. RCP-30-20A, Spherotech, Inc.) as described previously(Castillo-Hair et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Streptavidin-coated beads (2 μm diameter, Spherotech) were introduced into the chamber and incubated for 3 minutes to allow the beads to settle on the biotinylated coverslip surface ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µm and 3.4 µm polystyrene beads (Spherotech) were used for size calibration ...
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bioRxiv - Immunology 2019Quote: ... the raw data were converted to plate-calibrated ERFs using rainbow calibration particles (Spherotech). For a given cell population and readout ...
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bioRxiv - Bioengineering 2022Quote: ... which were purchased from Spherotech (Cat. No. FCM-4056-2).
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bioRxiv - Microbiology 2021Quote: To measure shedding of polystyrene beads (Spherotech FP-0552-2, sky blue), flow cytometry was used to quantify the number of egested beads ...
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bioRxiv - Neuroscience 2021Quote: ... voltages were set according to optimal PMT sensitivity using the peak 2 (Spherotech) voltration technique described previously by Maeker and Trotter 35 ...
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bioRxiv - Neuroscience 2024Quote: ... voltages were setup according to optimal PMT sensitivity using the peak 2 (Spherotech) voltration technique described previously by Maeker and Trotter64 ...
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bioRxiv - Immunology 2021Quote: ... serum and IL-2 starved OT1 CTLs were incubated with streptavidin polystyrene beads (Spherotech) coated with H-2Kb-OVA and ICAM-1 at a 1:1 ratio for various times at 37 ºC and immediately lysed in 2× cold lysis buffer containing phosphatase inhibitors (1 mM NaF and 0.1 mM Na3VO4 ...
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bioRxiv - Neuroscience 2022Quote: ... Tethers were pulled with a 4 µm diameter polystyrene bead (Spherotech #DIGP-40-2) held at the tip of a micropipette and controlled by micromanipulators ...
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bioRxiv - Neuroscience 2021Quote: ... voltages were set up according to optimal PMT sensitivity using the peak 2 (Spherotech) voltration technique described previously by Maeker and Trotter [46] ...
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bioRxiv - Neuroscience 2023Quote: ... Voltages were set up according to optimal PMT sensitive using the peak 2 (Spherotech) voltration technique ...
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bioRxiv - Microbiology 2021Quote: ... Nile Red nominally 5 µm (5-7.9 µm) (Spherotech, FP-6056-2, 1.5 × 108/ml) and Nile Red nominally 10 µm (10-14 µm ...
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bioRxiv - Cell Biology 2023Quote: ... We then incubated each sample with 0.22-μm light yellow beads (Spherotech, FP-0245-2) resuspended in 2× SSC as fiducial markers for drift correction and washed the sample with 2× SSC briefly.
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bioRxiv - Bioengineering 2023Quote: ... Rainbow Calibration particles (RCP-60-5-2, RCP-60-5-5) were obtained from Spherotech. FITC and PE stained beads were part of the three color BD CaliBRITE kit (BD Biosciences 340486) ...
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bioRxiv - Biophysics 2022Quote: ... and a 2.12 µm polystyrene bead coated in anti-digoxigenin antibody (Spherotech cat# DIGP-20-2) was caught in trap 2 which is upstream in the path of buffer flow to trap 1 ...
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bioRxiv - Microbiology 2021Quote: ... and Nile Red nominally 10 µm (10-14 µm) (Spherotech, FH-10056-2, 107 microspheres/ml) with unmodified surfaces ...
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bioRxiv - Biophysics 2024Quote: ... in Trap1 and 2.12-µm SPHERO Anti-Dig polystyrene beads (Spherotech, Cat. No. DIGP-20-2) in Trap2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... to enable conversion of GFP fluorescence into calibrated MEFL units 58 a single well per plate contained 15 μL of 8-peak Rainbow Calibration Particles (Spherotech, RCP-30-5A) diluted into 200 μL PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.8 μl of streptavidin-coated polystyrene beads (SA beads, 1% v/v suspension, Ø 2 μm, Spherotech) were mixed with 1 ml of assay buffer ...
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bioRxiv - Biophysics 2020Quote: ... A 10 μl reaction containing 3.5 μL of annealed complex and 1.6 μL of 0.5% streptavidin coated polystyrene beads (2 μm diameter, Spherotech) was incubated on ice for 30 min ...
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bioRxiv - Bioengineering 2023Quote: ... The Ultra Rainbow Calibration bead kit (Spherotech URCP-38-2k) consists of 3.8 µm polystyrene particles which contain embedded six different fluorochromes that align to the most common flow cytometry excitation and emission filter sets ...
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bioRxiv - Biophysics 2019Quote: The 8.1 kbp dsDNA construct with a primer-template junction at one terminus was tethered between 2 μm anti-digoxigenin and 3 μm streptavidin functionalized beads (Spherotech) held in place by a micropipette tip and a dual beam optical trap ...
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bioRxiv - Immunology 2023Quote: ... SA was then conjugated to spherotech 4μm and 5μm Carboxy Blue Particle Array Kits (Spherotech CPAK-4067-8K and CPAK-5067-10K) using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC ...
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bioRxiv - Immunology 2021Quote: ... and following surface staining cells were fixed and permeabilized according to the manufacturer’s instructions using the BD Cytofix/Cytoperm Plus Kit (BD. For counting the cells AccuCount Fluorecent particles (Spherotech, Lake Forest, IL, USA) were used ...
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bioRxiv - Immunology 2023Quote: Custom cytometric bead arrays were generated as previously described87 using 4 µm and 5 µm carboxy functionalized array kits (Spherotech, PAK-4067-8K and PAK-5067-10K). Streptavidin (SA ...