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Citations for Avanti Polar Lipids :
1 - 50 of 76 citations for hsa mir 146a Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... Thiolated miR-145 was conjugated to DSPE-PEG(2000)-malemide (Avanti Polar Lipids, Alabaster, AL) via a thioether bond by adding a 10% molar excess of lipid to reduced thiolated miR in DEPC-treated water ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting liposomes were extruded five times through a 200 nm polycarbonate membrane and five times through a 100 nm membrane (Avanti Polar Lipids) using an Avanti Mini Extruder (Avanti Polar Lipids) ...
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bioRxiv - Neuroscience 2019Quote: ... the size was reduced by extrusion though 800nm and 200nm polycarbonate filters 5 times each then 20-40 times through 100nm membranes using a mini-Extruder (Avanti Polar Lipids, Alabaster, AL).
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bioRxiv - Biophysics 2022Quote: ... and extruded 11 times through 400 nm polycarbonate membranes (Avanti Polar Lipids) by a syringe extruder (Avanti Polar Lipids) ...
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bioRxiv - Cell Biology 2020Quote: ... and extruded 25 times through polycarbonate filters (pore diameters, 200 nm; Avanti Polar Lipids) in a mini-extruder (Avanti Polar Lipids ...
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bioRxiv - Plant Biology 2023Quote: ... Lipids were identified by retention time compared to standards purchased from Avanti Polar Lipids. Remaining analysis was precisely done as described in Barnes et al 2016 (12)
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bioRxiv - Biophysics 2021Quote: ... The lipid suspension was subsequently extruded 19 times through a mini-extruder (Avanti Polar Lipids) equipped with a 200 nm polycarbonate membrane ...
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Observation of an α-synuclein liquid droplet state and its maturation into Lewy body-like assembliesbioRxiv - Molecular Biology 2020Quote: ... the mixture was extruded 20 times through 50 nm-diameter polycarbonate filters (Avanti Polar Lipids). Liposome size distribution was confirmed using dynamic light scattering (Average size ± 50 nm ...
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bioRxiv - Biophysics 2023Quote: ... Formed liposomes were extruded 21 times through 0.4 μm pores polycarbonate membranes (Avanti Polar Lipids). The extruded liposomes were stored in 4°C overnight.
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bioRxiv - Immunology 2022Quote: ... The resulting solution was extruded 21 times through a 200 nm membrane filter (Avanti Polar Lipids) using a 1 ml mini-extruder (Avanti Polar Lipids ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The resulting liposome dispersion was extruded 10 times (Avanti® Mini-Extruder, Avanti Polar Lipids, Inc.) using 0.2 μm polycarbonate membranes.
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bioRxiv - Cell Biology 2020Quote: ... lipid suspensions were extruded 25 times through polycarbonate filters (pore diameters, 200 nm; Avanti Polar Lipids) in a mini-extruder (Avanti Polar Lipids ...
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bioRxiv - Biophysics 2021Quote: ... following which the lipids were extruded 25 times through the mini extruder (Avanti Polar Lipids, Inc. USA) through cut off filter of 100nm polycarbonate membrane to prepare uniform Large unilamellar vesicles (LUV).
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bioRxiv - Biophysics 2022Quote: ... and passed through a 30-nm polycarbonate membrane 21 times using a mini-extruder (Avanti Polar Lipids) to generate small unilamellar vesicles ...
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bioRxiv - Biophysics 2020Quote: ... following which the lipids were extruded 25 times through the mini extruder (Avanti Polar Lipids, Inc. USA) through cut off filter of 100nm polycarbonate membrane to prepare uniform Large unilamellar vesicles (LUV) ...
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bioRxiv - Biophysics 2023Quote: ... The resuspended liposomes were then extruded 21 times through 0.1 μm pores polycarbonate membranes (Avanti Polar Lipids). The liposomes were stored in 4°C and used for the maximum of one week.
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bioRxiv - Biophysics 2021Quote: ... which was extruded through 0.2 µm membrane filters for 35 times using a mini-extruder (Avanti Polar Lipids). CorA was inserted by mixing a sample of 10.5 mg/ml LUVs ...
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bioRxiv - Biophysics 2022Quote: ... the mixture was extruded 15 times through a 100 nm membrane using the Mini Extruder (Avanti Polar Lipids). This yielded a liposome stock solution of 100 nm liposomes with 4.0 mM lipid in 10 mM Tris pH 7 ...
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bioRxiv - Microbiology 2021Quote: ... Lipid suspension was then passed 30 times through a 100 nm pore filter in an extruder (Avanti Polar Lipids). LUV’s were stored at 4 °C and used within 24h ...
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bioRxiv - Biophysics 2024Quote: ... and then extruded 17 times through a polycarbonate membrane with 100 nm pores using a miniextruder (Avanti Polar Lipids) at the same temperature ...
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bioRxiv - Biophysics 2021Quote: ... LUVs were prepared by extruding the 3.75 mM lipids 15 times through a 0.1 μm filter using a mini-extruder (Avanti Polar Lipids) and stored at 4°C until use.
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bioRxiv - Biophysics 2022Quote: ... and extruded through a 100 nm pore size filter 11 times by using a mini extruder (Avanti Polar Lipids, USA). The extruded sample was stored at 4°C until use.
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bioRxiv - Microbiology 2022Quote: ... The hydrated lipid films were then extruded 25 times using a handheld mini extruder (Avanti Polar Lipids, Alabaster, AL, USA) with a 100 nm pore diameter polycarbonate filter to obtain 100 nm LUVs ...
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bioRxiv - Biochemistry 2024Quote: ... The final proteoliposomes solution was then extruded by passing 11 times through a 400-nm membrane filter (Avanti Polar Lipids). These final proteoliposomes were kept at 4°C and used immediately ...
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bioRxiv - Biochemistry 2020Quote: ... The resuspended SUVs were then extruded 21 times through a 0.4 μm membrane followed by 21 times through a 0.1 μm membrane (Whatman, Nucleopore, UK) using the Mini Extruder from Avanti Polar Lipids Inc. ...
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bioRxiv - Biophysics 2022Quote: ... The lipid mixture was then extruded through a 100-nm polycarbonate membrane 21 times using a mini-extruder (Avanti Polar Lipids). Lipid vesicles were used immediately following extrusion.
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bioRxiv - Cell Biology 2022Quote: ... The multi-lamellar vesicles suspensions were frozen and thawed five times and then extruded through polycarbonate filters of 0.2 μm pore size using a mini-extruder (Avanti Polar Lipids). Liposomes were stored at 4°C and in the dark when containing fluorescent lipids and used within 2 days.
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bioRxiv - Biophysics 2022Quote: ... The solution was freeze-thawed at least 6 times under liquid nitrogen and then extruded 21 times using an Avanti pressure extruder with Whatmanfilter paper of size 0.1 μm (Avanti Polar Lipids Alabama ...
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bioRxiv - Biochemistry 2019Quote: ... This freeze-thaw cycle was repeated 5 times and then the thawed solution was extruded 21 times through a 0.1 μM filter using a mini-extruder (Avanti Polar Lipids).
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bioRxiv - Biochemistry 2021Quote: ... The multi-lamellar vesicles suspensions were frozen and thawed five times and then extruded through polycarbonate filters of 0.2 µm pore size using a mini-extruder (Avanti Polar Lipids). Liposomes were stored at 4°C and in the dark when containing fluorescent lipids and used within 2 days.
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bioRxiv - Biochemistry 2022Quote: ... The rehydrated lipid solutions were extruded 21 times through a 100 nm diameter pore size polycarbonate membrane (Avanti Polar Lipids Inc.). The extruded solutions were stored at 4°C and consumed within a week after preparation.
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bioRxiv - Biophysics 2023Quote: ... The solutions were then extruded at least 21 times through a 200 nm cut off polycarbonate membrane filter (Avanti Polar Lipids). Vesicles were then filtered using PD-10 desalting columns (Sigma Aldrich ...
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bioRxiv - Synthetic Biology 2023Quote: ... The resulting vesicles were extruded ten times through a 100 nm polycarbonate membrane using a commercial mini-extruder (Avanti Polar Lipids).
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bioRxiv - Cell Biology 2020Quote: ... were extruded 25 times through polycarbonate filters (pore diameters of 50, 100, 200, or 400 nm) in a mini-extruder (Avanti Polar Lipids) at 40°C ...
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bioRxiv - Microbiology 2019Quote: ... The sample was then extruded 11 times (65 °C) through polycarbonate membrane (pore size of 200 nm) with a syringe extrusion device (Avanti Polar Lipids) after which the sample was quickly cooled down and stored in a refrigerator ...
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bioRxiv - Cell Biology 2019Quote: ... The resulting LUV solution was passed more than 20 times through a polycarbonate filter membrane with a pore size of 100 nm using the extruder from Avanti Polar Lipids, Inc ...
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bioRxiv - Biophysics 2020Quote: ... Uni-lamellar vesicles were formed via ten cycles of freeze-thawing followed by extrusion ten times) through a polycarbonate membrane with pores sizes of 100 nm using a mini-extruder (Avanti Polar Lipids) kept 50 °C on a hotplate ...
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bioRxiv - Biophysics 2022Quote: ... the liposome solution was passed 11 times through a polycarbonate membrane with a 400 nm pore size in a mini extruder (Avanti Polar Lipids). Total lipid concentration was diluted with buffer to 0.16 mg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... Unilamellar liposomes were produced by seven freeze-thaw cycles (30 sec in liquid nitrogen followed by 5 min in a 37°C water bath) and extrusion (at least 21 times) through a polycarbonate filter with 100 nm pore size (polycarbonate membranes from Avanti Polar Lipids). The liposomes were then stored on ice.
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bioRxiv - Bioengineering 2022Quote: ... smegmatis and then co-extruded nine times through a 100 nm pore size polycarbonate porous membrane using a mini extruder (Avanti Polar Lipids). Excess OMVs and soluble compounds were removed from the supernatant after centrifugation at 15000 g for 10 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... using established multiple reaction monitoring pairs and retention times were compared to repeated injections of OxysterolSPLASH™ standard mix (Avanti Polar Lipids) in an extraction control sample of rhesus BAL ...
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bioRxiv - Biophysics 2022Quote: ... The resulting MLV suspension was then sonicated for 10 min to disperse larger aggregates and the liposomal suspension was extruded 21 times through polycarbonate filters (100 nm pore size, Avanti Polar Lipids) using a mini-extruder (Avanti Polar Lipids) ...
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bioRxiv - Cell Biology 2019Quote: ... The lipid suspensions obtained were then extruded 25 times through polycarbonate filters (pore diameters, 400, 800, or 1000 nm; Avanti Polar Lipids) in a mini-extruder (Avanti Polar Lipids ...
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bioRxiv - Pathology 2021Quote: ... Liposomes were extruded through a polycarbonate filter (400 nm or 100 nm) for 25 times using a mini-extruder (Avanti Polar Lipids). Purified AvrE protein was incubated with the liposome at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... Liposomes were then extruded 21 times through a polycarbonate membrane with a 50◻nm pore size (Mini-Extruder, Avanti Polar Lipids). Extruded liposomes were centrifuged at 18,000 g for 5 min to remove insoluble material and stored at 4°C ...
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bioRxiv - Immunology 2021Quote: ... liposomes were passed at least 15 times through 800-nm and 100-nm filters using a hand-held extrusion device (Avanti Polar Lipids). Excess Protein-PEG2000-DPSE was separated from liposomes using a CL-4B Sepharose (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... Crude liposomes were then extruded through a polycarbonate filter with 100 nm pore size a total of 11 times via a mini extruder (Avanti Polar Lipids) and used within 24 hours.
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bioRxiv - Biochemistry 2022Quote: ... The liposomes were homogenized by extruding 11 times through a 50 nm polycarbonate filter (Nuclepore Track-Etched Membranes, Whatman, Maidstone, UK) using a Mini Extruder instrument (Avanti Polar Lipids). QCM-D experiments were conducted with an X4 instrument equipped with a flow chamber (AWSensors ...
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bioRxiv - Molecular Biology 2023Quote: ... The lipid suspension was incubated at room temperature for 30 min and extruded 20 times through a polycarbonate 0.4-μm filter using a mini-extruder (Avanti Polar Lipids, Inc.).
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bioRxiv - Plant Biology 2023Quote: ... To control the liposome size, liposomes were extruded through a polycarbonate filter (200 nm, Whatman) 25 times using a mini extruder (Avanti Polar Lipids) with Hamilton glass syringes ...