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Citations for Avanti Polar Lipids :
301 - 350 of 447 citations for Vanin 1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and DOGS-NTA-Ni2+ (1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl) iminodiacetic acid) succinyl]) were purchased from Avanti Polar Lipids. Glyceryl trioleate was purchased from Sigma-Aldrich
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bioRxiv - Biophysics 2020Quote: ... and 2% 1,2-dioleoyl-sn-glycero-3-[N(5-amino-1-carboxypentyl)iminodiaceticacid]succinyl[nickel salt] (Ni-DOGS NTA) (Avanti Polar Lipids) were prepared at a total lipid concentration of 0.5mg ml−1 as described 22 in 10x Dulbecco’s phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2021Quote: ... was combined with 0.8% 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl)iminodiacetic acid)succinyl] (nickel salt) (DGS-NTA[Ni]) (Avanti Polar Lipids), dried with N2 and incubated for 1 hour in a vacuum desiccator ...
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bioRxiv - Biochemistry 2020Quote: ... the vesicles were composed of a 1:1.5 ratio of the lipids DOGS-NTA-Ni2+ (1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl) iminodiacetic acid)succinyl] (nickel salt) and DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine) (Avanti Polar Lipids); after hydration and five freeze-thaw cycles ...
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bioRxiv - Microbiology 2020Quote: ... The suspension was extruded through polycarbonate membranes with 1 or 0.2 µm pore size using a mini-extruder (Avanti Polar Lipids) to create large or small unilamellar vesicles (LUV/SUV) ...
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bioRxiv - Pathology 2022Quote: ... and supernatants were supplemented with NP-40 (1%, Hölzel Diagnostika Handels GmbH #P-1505) and 1,2-diheptanoyl-sn-glycero-3-phosphocholine (DHPC, 30mM, Avanti Polar Lipids ##850306P), incubated (5min. ...
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bioRxiv - Cell Biology 2022Quote: ... Peptide fractions were pooled then incubated at a molar ratio of 1:1.1 (excess lipid) with MPB-PE (Avanti Polar Lipids) for 3- 12 hours at 25C with end-over-end mixing ...
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bioRxiv - Biophysics 2022Quote: ... Reconstitution of IP3Rs into lipid-nanodiscs occurred “on-column” as we described in 26: 1.2 mM POPC (1-palmitoyl-2-oleoyl-glycero-3-phosphocholine, Avanti Polar Lipids) prepared in 3% DDM (n-dodecyl-β-d-maltoside ...
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bioRxiv - Cell Biology 2022Quote: ... and 5% 18:1 (0.33 mM final) PI phosphates (PI(3,5)P2 or PI(4,5)P2) (Avanti Polar lipids, Alabaster, AL) were prepared according to (Banerjee and Kane ...
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bioRxiv - Bioengineering 2022Quote: ... PE) and 1,2-dioleyol-sn-glycero-3-phosphoethanolamine-N-(Cyanine-5) (18:1 Cy5 PE) were purchased from Avanti Polar Lipids. Absolute ethanol (200 proof) ...
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bioRxiv - Biophysics 2022Quote: ... and 1,2-Dipalmitoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl)iminodiacetic acid)succinyl] (nickel salt) were obtained from Avanti Polar Lipids. Lipid stocks were stored in chloroform under nitrogen at -20 °C.
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bioRxiv - Biochemistry 2019Quote: ... and the nickel-chelating lipid DGS-NTA-Ni2+ (1,2-dioleoyl-sn-glycero-3-{[N-(5-amino-1-carboxypentyl)-iminodiacetic acid]succinyl}) (Avanti Polar Lipids) mixed in a 1.5:1 ratio ...
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bioRxiv - Cancer Biology 2019Quote: ... and 4 mol % 1,2-Dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl)imidodiacetic acid)succinyl] (nickel salt) (DOGS-NTA-Ni; Avanti Polar Lipids) were mixed in chloroform in the flask ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl)iminodiacetic acid)succinyl] (DGS-NTA) were from Avanti Polar Lipids. Texas Red 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (DHPE-TexasRed ...
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bioRxiv - Biophysics 2020Quote: Purified GLIC protein was reconstituted into liposomes formed with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (PE, Avanti Polar Lipids) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1’-rac-glycerol ...
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bioRxiv - Physiology 2021Quote: ... A volume of 800 μL of chloroform:methanol (2:1 v/v) containing 15 μL internal standards (ISTDs, EquiSPLASH, Avanti Polar Lipids) were homogenized using the bead beating device (Precellys ...
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bioRxiv - Biophysics 2021Quote: ... and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-cap biotinyl (18:1 Biotinyl Cap PE; #870273, Avanti Polar Lipids, Alabaster, USA) were dissolved in chloroform (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... The final lipid extracts in chloroform:methanol (1:2, v:v) were spiked with internal phospholipid standards or Ceramide/Sphingoid Internal Standard Mixture I (Avanti Polar Lipids, USA) and 1% NH4OH (Merck ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 1,2-distearoyl-sn-glycero-3-phosphocholine and 18:1 (Δ9-Cis) PE (DOPE) 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine were purchased from Avanti Polar Lipids. MOPS 3-(4-morpholino ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mg of purified hZnT7 or hZnT7ΔHis-loop was added to 5 mg egg yolk phosphatidylcholine (egg PC, Avanti Polar Lipids) in 1 mL of PBS containing 0.8% sodium cholate (Sigma) ...
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bioRxiv - Biophysics 2022Quote: ... and 2 mol% 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino 1-carboxypentyl)iminodiacetic acid)succinyl] nickel salt (DGS-NTA, Avanti Polar Lipids) were prepared by sonicating a 0.5 mg/mL lipid suspension in water followed by centrifugation (21,000 g ...
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bioRxiv - Biophysics 2022Quote: ... 1,2-dioleoyl-sn-glycero-3-phospho-(1’-rac-glycerol) (DOPG) and (ii) soy asolectin lipid extract were from Avanti Polar Lipids and Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... To produce immobile PLBs we used 1% Ni-DOGS-NTA and 99% 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC, Avanti Polar Lipids) instead of POPC ...
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bioRxiv - Biophysics 2023Quote: ... and DGS-NTA-Ni (1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl) iminodiacetic acid)-succinyl] (nickel salt)) were purchased from Avanti Polar Lipids, Inc ...
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bioRxiv - Cell Biology 2023Quote: ... and DGS-NTA-Ni (1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl) iminodiacetic acid)-succinyl] (nickel salt)) were purchased from Avanti Polar Lipids. All reagents were used without further purification.
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bioRxiv - Synthetic Biology 2023Quote: ... and 100 µL of a 10 mg mL−1 solution of 1,2-dioleoyl-sn-glycero-3-phosphocholine lipids (DOPC, Avanti Polar Lipids) in chloroform ...
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bioRxiv - Neuroscience 2023Quote: ... and 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl)iminodiacetic acid)succinyl]-Ni2+ (DOGS-NTA-Ni2+) (Avanti Polar Lipids) were dissolved in chloroform and mixed in a glass tube at a molar ratio of 62:8 ...
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bioRxiv - Cell Biology 2023Quote: ... 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl)iminodiacetic acid)succinyl] (nickel salt, Ni-NTA-DOGS) (Avanti Polar Lipids), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (DSPE-PEG2000 ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl) iminodiacetic acid) succinyl] (nickel salt) (DGS-NTA (Ni)) (Avanti Polar Lipids) in chloroform were transferred to a glass vial for a final concentration of 500 μM lipid in oil with 95% POPC and 5% DGS-NTA (Ni ...
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bioRxiv - Biochemistry 2023Quote: ... Ceramidase activity was assessed by incubating 1.8 μM of purified SIDT1 and SIDT2 proteins with 40 μM ceramide (d18:1/18:0) (Cat# 860518P, Avanti Polar Lipids) at room temperature for 30 minutes ...
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bioRxiv - Biophysics 2024Quote: ... and DOPI(4,5)P2 (1,2-dioleoyl-sn-glycero-3-phospho-[1’-myo-inositol-4’,5’-bisphosphate] [ammonium salt]) were purchased from Avanti Polar Lipids. DPPI(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate diC16 ...
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bioRxiv - Biochemistry 2024Quote: ... 18:1 [Δ9-Cis] PC) and DOPS (1,2-dioleoyl-sn-glycero-3-phospho-L-serine) lipids (purchased from Avanti Polar Lipids) in chloroform or chloroform/methanol/water were mixed in a glass vial at molar ratio of 70:30 ...
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bioRxiv - Cell Biology 2023Quote: ... and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (18:1 Liss Phod PE) (810150C; Avanti Polar Lipids) were added to the mixtures to label the liposomes ...
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bioRxiv - Microbiology 2024Quote: ... PA (1,2-dioleoyl-sn-glycero-3-phosphate) and PI [1,2-dioleoyl-sn-glycero-3-phospho-(1’-myo-inositol)] were purchased from Avanti Polar Lipids. Liposomes PI3P(- ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 1-oleoyl-2-[12-biotinyl(aminododecanoyl)]-sn-glycero-3-phosphocholine (18:1-12:0 biotin PC) powder was purchased from Avanti Polar Lipids. The biotin-GD2 ...
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bioRxiv - Biophysics 2021Quote: ... and 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-arboxypentyl)iminodiacetic acid)succinyl] (nickel salt) (Ni-NTA-DGS) were from Avanti Polar Lipids, Inc ...
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bioRxiv - Immunology 2022Quote: ... 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl)iminodiacetic acid)succinyl] (DGS)–NTA and 1,2-dioleoyl-sn-glycero-3-phosphocholine (Avanti Polar Lipids, Inc.) were mixed at 1:3:96 molar % ratio ...
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bioRxiv - Microbiology 2020Quote: ... MLVs were prepared in a 3:1:1 ratio of 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC):1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS):1-palmitoyl-2-oleoyl-sn-glycero-3-phosphate (POPA) (Avanti Polar Lipids). Background signal in the absence of liposomes is due to protein aggregation during centrifugation.
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bioRxiv - Immunology 2021Quote: ... Briefly, freshly isolated PBMCs (1 × 106 cell/ml, 5 ml/well) were treated with 100 ng/ml αGalCer (KRN7000, Avanti Polar Lipids) and cultured for 13 days ...
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bioRxiv - Biophysics 2019Quote: ... The vesicle solution consisted of 1 mg/ml of 95% POPC and 4.999% DGS-NTA(Ni) and 0.001% Biotinyl-Cap-PE (all Avanti Polar Lipids, Alabaster USA). After incubating for 30 minutes the wells were washed three times with PBS solution and the protein solution added at 30 μg/ml ...
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bioRxiv - Biophysics 2020Quote: ... 1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphoinositol (PI(18:0/20:4)) and 1-stearoyl-2-linoleoyl-sn-glycerol (DG(18:0/18:2)) were purchased from Avanti Polar Lipids.
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bioRxiv - Immunology 2019Quote: ... and 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl) iminodiacetic acid) succinyl] (nickel salt, DGS-NTA-Ni, catalog 790404C) were purchased from Avanti Polar Lipids. LUVs composing of 80% POPC and 20% DGS-NTA-Ni were generated by the extrusion method ...
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bioRxiv - Biophysics 2019Quote: ... lipid and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (18:1 lissamine rhodamine PE) were purchased from Avanti Polar Lipids Inc ...
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bioRxiv - Microbiology 2019Quote: ... The membrane pellet was resuspended in 30 mL Buffer A supplemented with 1% n-Dodecyl β-D-maltoside (DDM, Avanti Polar Lipids), homogenized with a Dounce homogenizer ...
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bioRxiv - Biochemistry 2019Quote: ... 1-hexadecanoyl-2-(5’-oxo-valeroyl)-sn-glycero-3-phosphocholine (POVPC) and 1-hexadecanoyl-2-(9’oxononanoyl)-sn-glycero-3-phosphocholine (PONPC) were procured from Avanti Polar Lipids, USA.
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bioRxiv - Biochemistry 2019Quote: ... 1-stearoyl-2-linoleyl-sn-glycero-3-phosphocholine (SLPC) and 1-stearoyl-2-arachidonyl-sn-glycero-3-phosphocholine (SAPC) were procured from Avanti Polar Lipids, Inc ...
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bioRxiv - Biochemistry 2020Quote: Freshly purified ASCT2 was reconstituted in the liposomes composed of Escherichia coli polar lipids and egg phosphatidylcholine at a 3:1 ratio (w/w) and supplemented with 10% (w/w) cholesterol (Avanti Polar Lipids)20 ...
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bioRxiv - Neuroscience 2021Quote: ... and 1,2-dioleoyl-sn-glycero-3-phospho-(1′-myo-inositol-4′,5′- bisphosphate) (PI(4, 5)P2) were obtained from Avanti Polar Lipids. HEPES was from Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... functional ChRmine at high density into phospholipid vesicles consisting of a 10:1 mixture of chicken egg yolk phosphatidylcholine (egg PC; Avanti Polar Lipids) and the adjuvant lipid A (Sigma-Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... and 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl)iminodiacetic acid)succinyl] (nickel salt) (Ni2+-NTADOGS) were purchased from Avanti Polar Lipids. Alexa Fluor 488 ...