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Citations for Avanti Polar Lipids :
1 - 50 of 56 citations for Poloxamer 188 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Evolutionary conserved aspects of animal nutrient uptake and transport in sea anemone vitellogenesisbioRxiv - Evolutionary Biology 2022Quote: Stock solution of alkyne-modified oleic acid (17-yne) (Avanti Polar Lipids, ethanol solution 1mg/ml) was aliquoted ...
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bioRxiv - Molecular Biology 2023Quote: ... A solution of DSPE-PEG2000 (Avanti Polar Lipids, USA) in CHCl3 was added to the lipid mixture and organic solvents were removed in vacuo ...
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bioRxiv - Microbiology 2023Quote: ... all purchased as chloroform solution from Avanti Polar Lipids, Inc ...
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bioRxiv - Biochemistry 2024Quote: ... A stock solution of DOPC in chloroform (10 mg/mL) had previously been prepared from a 25 mg/mL solution (Avanti Polar Lipids #850375C). The following steps all took place in glass-vials covered in aluminium foil ...
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bioRxiv - Biophysics 2024Quote: ... A solution of 1% biotinyl cap-phosphoethanolamine (Avanti Polar Lipids, 870277P) in Egg PC (Avanti Polar Lipids ...
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bioRxiv - Immunology 2023Quote: ... solutions of cholesterol (20mg/ml final concentration, Avanti Polar Lipids Cat# 700000), DPPC (20mg/ml final concentration ...
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bioRxiv - Biochemistry 2024Quote: Chloroform Lipid solutions (DOPC or DOPS) were purchased from Avanti Polar Lipids Inc ...
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bioRxiv - Biochemistry 2021Quote: ... This lipid solution was extruded through 100 nm polycarbonate membrane (Avanti Polar Lipids) to get liposomes of 100 nm range ...
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bioRxiv - Biophysics 2022Quote: ... The solution is then moved to an extruder set (Avanti Polar Lipids 610000) kept on a hot plate to maintain its temperature above the phase transition temperature of DPPC ...
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bioRxiv - Biophysics 2022Quote: ... This colloidal heated solution was subjected to extrusion (Mini Extruder, Avanti Polar Lipids) using 200 nm pore-sized polycarbonate membranes ...
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bioRxiv - Physiology 2023Quote: ... into which 10 μL internal standard solution (SPLASH® Lipidomix®, Avanti Polar Lipids) and 120 μL methanol were added ...
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bioRxiv - Genetics 2023Quote: ... into which 10 µL internal standard solution (SPLASH® Lipidomix®, Avanti Polar Lipids) and 120 µL methanol were added ...
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bioRxiv - Biophysics 2024Quote: ... stock solutions of each lipid component stored in chloroform purchased from Avanti Polar Lipids, Inc were mixed in chloroform:methanol (3:1 ...
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bioRxiv - Biophysics 2024Quote: ... the solution was extruded 31 times using a Mini-Extruder kit (Avanti Polar Lipids) with 1 Whatman 100 nm filter (GE Healthcare Life Sciences ...
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bioRxiv - Biophysics 2023Quote: Lipid stock solutions of DOTAP and DOPC in chloroform were purchased from Avanti Polar Lipids at 25 mg/mL (35.8 and 31.8 mM ...
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bioRxiv - Immunology 2022Quote: ... The resulting solution was extruded 21 times through a 200 nm membrane filter (Avanti Polar Lipids) using a 1 ml mini-extruder (Avanti Polar Lipids ...
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bioRxiv - Bioengineering 2020Quote: ... a lipid solution containing 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) (Avanti Polar Lipids, Alabaster, AL), 1,2-diplamitoyl-sn-glycero-3-phosphate (DPPA ...
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bioRxiv - Biophysics 2020Quote: ... a solution of 1 mg/mL DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine, Avanti Polar Lipids) dissolved in 1:2 Methanol:Chloroform was spin coated at 3200 rpm for 45 seconds on a 25 mm diameter cover slip ...
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bioRxiv - Biophysics 2023Quote: ... The resulting solution was then extruded with a Mini Extruder (Avanti Polar Lipids, Alabaster, AL, USA), 21 passes each through a 200nm and 100nm poly-carbonate Nuclepore Track-Etched Membrane (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: Supported lipid bilayers were assembled using the SUV solution of DOPC (Avanti Polar Lipids; cat no. 850375) doped with 2% SUV solution of 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-cap biotinyl (16:0 Biotinyl Cap PE ...
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bioRxiv - Biophysics 2023Quote: ... the concentrated stock solution of n-dodecyl-β-D-maltoside (DDM Avanti Polar Lipids, Inc, Alabaster, AL) were added to the 5 mg/mL of freshly extruded LUVs solution to reach a final concentration of 80 mM ...
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bioRxiv - Biophysics 2022Quote: ... of synthetic POPC (10 mg/ml) and POPE (10 mg/ml) stock solutions (Avanti Polar Lipids, Birminham, AL), respectively ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The PA solution was prepared by re-constituting purified egg PA extract (Avanti Polar Lipids, Alabaster, AL, USA) at 10 mg/mL in 10% DMSO in 1x PBS ...
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bioRxiv - Biophysics 2022Quote: ... L-α-phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) (Brain, Porcine) were purchased as chloroform (or chloroform:methanol:water) solutions from Avanti Polar Lipids Inc ...
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bioRxiv - Biophysics 2022Quote: ... This solution is then passed through a 100nm Nucleopore Track-Etched Membranes in an extruder (Avanti Polar Lipids), and sonicated.
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bioRxiv - Neuroscience 2024Quote: ... Patch pipette solution was used as a solvent for dioctanoyl-phosphatidic acid (Avanti Polar Lipids, Cat. Nu. 830842P), palmitoyl-LPA (Cayman Chemical ...
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bioRxiv - Biophysics 2021Quote: ... The solution was then extruded through a polycarbonate filter (0.05 μm) using a hand-held extruder (Avanti Polar lipids) to a final concentration of 1 mg mL−1 ...
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bioRxiv - Biophysics 2024Quote: ... The lipid solutions were further extruded through 1μm and 200 nm pore-size polycarbonate membranes (PC Membranes 0.2μm, Avanti Polar Lipids) 15 times to form Giant unilamellar vesicles (GUVs ...
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bioRxiv - Cell Biology 2024Quote: A 50 μL methanol solution containing 20 pmol each of C15:0/18:1 PA (Avanti Polar Lipids, #330721) and C12:0/13:0 PE (Avanti Polar Lipids ...
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bioRxiv - Biophysics 2020Quote: ... Bilayers were prepared from SUV solutions containing POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, Avanti Polar Lipids, 850457C) and 0.01% Oregon Green DHPE (1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine ...
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bioRxiv - Biophysics 2022Quote: ... A mixture of 17.62 µL of the stock DPPC solution (25 mg/mL DPPC in chloroform, Avanti Polar Lipids 850355C) and 15.46 µL of 10 mg/mL cholesterol (SigmaAldrich C8667 ...
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bioRxiv - Biochemistry 2024Quote: ... The final proteoliposomes solution was then extruded by passing 11 times through a 400-nm membrane filter (Avanti Polar Lipids). These final proteoliposomes were kept at 4°C and used immediately ...
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bioRxiv - Biochemistry 2024Quote: A 2 mM stock solution of DHE in absolute ethanol was prepared from 1 mg solid (Avanti Polar Lipids, #810253). Dansyl-PE (1 mL ...
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bioRxiv - Cell Biology 2024Quote: ... The remaining cell resuspension was added to ice cold extraction solution (600 μL acidified methanol [0.1% HCl final], 250 μL hexane, with 0.1 μM CoQ8 internal standard [Avanti Polar Lipids]). Samples were vortexed and centrifuged (5 min ...
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bioRxiv - Biophysics 2021Quote: ... and covered with a lipid/oil solution containing 9.5 mg/ml 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC, Avanti Polar Lipids, USA) and 1:1 (v:v ...
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bioRxiv - Immunology 2020Quote: ... After vortexing the mixture, internal standard solution (either, SPLASH® Lipidomix® Mass Spec Standard (330707, Avanti Polar Lipids, Alabaster, US) or standard mixture according to Table II ...
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bioRxiv - Biochemistry 2022Quote: ... The rehydrated lipid solutions were extruded 21 times through a 100 nm diameter pore size polycarbonate membrane (Avanti Polar Lipids Inc.). The extruded solutions were stored at 4°C and consumed within a week after preparation.
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bioRxiv - Biophysics 2024Quote: ... The resulting multilamellar vesicle solutions were extruded through a 100 nm diameter polycarbonate membrane in a Mini extruder (Avanti Polar Lipids). The LUVs were prepared in 50 µM NaCl solution ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 100 µL of a 10 mg mL−1 solution of 1,2-dioleoyl-sn-glycero-3-phosphocholine lipids (DOPC, Avanti Polar Lipids) in chloroform ...
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bioRxiv - Neuroscience 2024Quote: ... 25 µL of a precipitation solution containing isotopically labeled internal standards (IS; all purchased from Avanti polar lipids, Alabaster, AL, USA) was added ...
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bioRxiv - Microbiology 2024Quote: ... followed by 15 cycles of extrusion through 0.2 µm nucleopore polycarbonate membranes (Cytiva Global Life Sciences Solutions, USA) mounted in a mini-extruder (Avanti Polar Lipids). Calcein-filled LUVs were separated from free calcein by size-exclusion chromatography (Sephadex G-50 ...
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A gated hydrophobic funnel within BAX binds long-chain alkenals to potentiate pro-apoptotic functionbioRxiv - Cell Biology 2024Quote: Hexadecanal (16:0 aldehyde) was purchased as 1 mg/ml (4.16 mM) in methylene chloride solution (Cat. No. 857458M, Avanti Polar Lipids), aliquoted into 25 μl ...
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bioRxiv - Biophysics 2020Quote: ... and 1-palmitoyl-2-{6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl}-sn-glycero-3-phosphocholine (NBD-PC)) were obtained as stock solutions in chloroform from Avanti Polar Lipids. Bio-Beads SM2 resin was from Bio-Rad Laboratories ...
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bioRxiv - Biophysics 2022Quote: ... the liposome solution was passed 11 times through a polycarbonate membrane with a 400 nm pore size in a mini extruder (Avanti Polar Lipids). Total lipid concentration was diluted with buffer to 0.16 mg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... [27] in a mixture of CHCl3–CH3OH (1:1 vol.) was added to a solution of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE, Avanti Polar Lipids, USA) in CHCl3 at a molar ratio of 1:3 and gently stirred ...
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bioRxiv - Microbiology 2024Quote: ... the final lipid solution (20 mg ml-1) was extruded at least ten times through a Mini Extruder (Avanti Polar Lipids, USA) equipped with polycarbonate membranes of 0.1 μm pore size ...
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bioRxiv - Biophysics 2023Quote: ... 100 mol %) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) (both purchased as chloroform solutions from Avanti Polar Lipids, Alabaster, AL) were dissolved in chloroform to a concentration of 4 mM ...
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bioRxiv - Biophysics 2023Quote: ... Bilayers were built by adding 10μl of a 1,2-diphytanoyl-sn-glycero-3-phosphocholine solution (DPhPC, 10mg/ml, Avanti Polar Lipids, Alabama, United States) in pentane onto each buffer reservoir and pipetting up and down as described previously 42 ...
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bioRxiv - Bioengineering 2024Quote: ... 990 µl of a 2 mM lipid solution was coextruded with 10 µl of a 2 mM SMALP solution through a 100 nm polycarbonate membrane with a mini extruder (Avanti Polar Lipids, USA). Resulting LUVs were washed 2 times by centrifugation (100 000g for 30 min).
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bioRxiv - Biophysics 2024Quote: ... SUVs were prepared using tip sonication of a HEPES solution of 5 mol % DOPE-cap-B and 95 % DOPC (both Avanti Polar Lipids, USA). At the start of the RICM image acquisition ...