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Citations for Avanti Polar Lipids :
201 - 231 of 231 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... a biphasic extraction mixture consisting of 225 μL methanol and 750 μL methyl tert-butyl ether containing 76 internal lipid standards (Avanti Polar Lipids UltimateSPLASH ONE kit plus acylcarnitines and free fatty acids ...
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bioRxiv - Cell Biology 2024Quote: ... adherent cells were washed and scraped into 500μL of methanol and spiked with deuterated internal standards: Equisplash (Avanti Polar Lipids, Cat# 330731), SPB 18:0;O2 {d7] (Avanti Polar Lipids ...
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bioRxiv - Cancer Biology 2024Quote: ... The dried sample was reconstituted using acetonitrile/isopropanol/water 65:30:5 containing stable isotope labeled internal lipid standards (Splash Lipidomix, Avanti Polar Lipids) prior to LC-MS analysis ...
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bioRxiv - Biophysics 2023Quote: ... dried lipid vesicles made of 1,2-Dioleoyl-sn-Glycero-3-phosphoethanolamine-N-[Methoxy(Polyethyleneglycol)-5000] (ammonium salt) (DOPC) (Avanti Polar Lipids, product number 880230, Alabaster, AL) were suspended in a buffer containing 10 mM Tris-HCl (pH 7.8 ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Lipids were extracted from plasma or tissue homogenates (10 µL) using 190 µL of isopropanol containing 1,2-dilauroyl-sn-glycero-3-phosphocholine as an internal standard (Avanti Polar Lipids; Alabaster, AL). After centrifugation (10 min ...
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bioRxiv - Physiology 2023Quote: The standards (PC_36:4 and PE_34:2) and the isotope labeled internal standards (PC_33:1-d7 and PE_31:1-d7) were purchased from Avanti Polar Lipids (Alabaster, Alabama, USA). The Standard solution at concentrations of 0 ...
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bioRxiv - Cell Biology 2024Quote: Cellular lipids for OzID-mass spectrometry and sum composition analyses were extracted from cells according to previously described literature28 and were quantified using deuterated lipid internal standards (SPLASH Lipid-o-mix®, Avanti Polar Lipids). In short ...
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bioRxiv - Neuroscience 2023Quote: ... Czech Republic). Lipidomics standards SPLASH Lipidomix (cat. #330707) and Cer/Sph Mixture I (cat. #LM6002) were purchased from Avanti Polar Lipids (Alabaster, USA). 3- sulfopropionic acid (cat ...
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bioRxiv - Biochemistry 2023Quote: ... for which 20 µL of mouse serum was combined with 180 µL of chloroform:methanol extraction solvent (v:v=1:2) containing internal standards at the final concentrations: 5nM D7-Sphinganine (Avanti Polar Lipids Inc., Cat# 860658), 12.5nM D3-Deoxysphinganine (Avanti Polar Lipids Inc. ...
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bioRxiv - Cell Biology 2022Quote: Prior to the lipid extraction samples were thawed on ice and spiked with SPLASH LIPIDOMIX deuterium-labeled internal standards (Avanti Polar Lipids Inc.). The lipids were extracted from samples using a modified Bligh and Dyer total lipid extraction method (Bligh and Dyer ...
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bioRxiv - Biochemistry 2020Quote: Cell and homogenised tissue lipids were extracted using methods similar to those described by Matyash et al.4 and were quantifiable through the use of internal standards in the form of deuterated lipids (SPLASH Lipid-o-mix, Avanti Polar Lipids, Alabaster, USA) and an odd-chain fatty acid found to be not present within the samples (nonadecanoic acid ...
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bioRxiv - Cell Biology 2022Quote: ... Internal standards (ISTD) added to the tissues before extraction included 500 pmol (20 μl) of Cer/Sph Mixture I (Avanti Polar Lipids LM-6002), and 1nmol of C12 Sphingosyl PE (C17:1/C12:0 ...
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bioRxiv - Cell Biology 2022Quote: ... Internal standards (ISTD) added to the tissues before extraction included 500 pmol (20 μl) of Cer/Sph Mixture I (Avanti Polar Lipids LM-6002), and 1nmol of C12 Sphingosyl PE (C17:1/C12:0 ...
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bioRxiv - Molecular Biology 2020Quote: ... Data were corrected for differences in extraction efficiency using the recovery of the DNP-PE internal standard (Avanti Polar Lipids, Inc., Alabaster, AL; USA).
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bioRxiv - Biochemistry 2022Quote: ... directly into a 96-well plate preloaded with methanol spiked with 0.01% BHT and internal standards (PC 17:0/17:0 and dihydrosphingomyelin, DHSM, 12:0; Avanti Polar Lipids, Alabaster, AL, USA). Internal standards were added at a rate of 1000 fmol per well for fifty cells and 1.38 fmol per single cell ...
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bioRxiv - Neuroscience 2022Quote: ... The tissue was added to low retention Eppendorf tube filled with 0.6 mL 80% methanol (MeOH) and 10 μL on internal standard mix (SPLASH® LIPIDOMIX #330707 from Avanti Polar Lipids, Alabaster, AL) and kept in dry ice ...
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bioRxiv - Pathology 2023Quote: ... exact mass peaks corresponding to H+ adducts from standards of C18:1 (Δ-cis) carnitine oleoyl L-carnitine (Avanti Polar Lipids, Inc., Alabaster, AL) and 16:0-18:1 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (Avanti Polar Lipids ...
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bioRxiv - Neuroscience 2023Quote: ... Glucosylceramide and galactosylceramide were identified based on their retention times and MRM properties of commercially available reference standards (Avanti Polar Lipids, Birmingham, AL, USA). Table S4 shows specific analytes and internal standards used in this assay.
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bioRxiv - Neuroscience 2024Quote: ... Each cell pellet was resuspended in 500 µL of resuspension buffer (0.05 M HCl, 49% Methanol, 1% SPLASH Lipidomic Mass Spec Standard [Avanti Polar Lipids, Cat. Nu. 330707]), and 1 mL methyl-tert-butyl-ether was added ...
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bioRxiv - Cell Biology 2023Quote: ... Mouse epidermis or skin equivalents were weighed and lipids extracted with a chloroform/methanol mixture 2:1 after addition of the internal standard mixture containing SPLASH Lipidomix®,-LM6002 and d31Cer[NS] 34:1 (Avanti Polar Lipids, USA), and in-house mixed deuterated standards supplied by C/D/N isotopes and Toronto Research Chemicals ...
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bioRxiv - Cell Biology 2024Quote: ... adherent cells and tumor punches were washed and scraped or homogenized in 500uL of methanol and spiked with deuterated internal standards: Ultimate splash (Avanti Polar Lipids, Cat#330820), SPB 18:0;O2 [D7] (Avanti Polar Lipids ...
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bioRxiv - Cancer Biology 2021Quote: Cell lipids were extracted using methods similar to those described by Matyash et al.65 and were quantifiable through the use of internal standards in the form of deuterated lipids (SPLASH Lipid-o-mix®, Avanti Polar Lipids, Alabaster, USA). To minimize pipetting error ...
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bioRxiv - Neuroscience 2022Quote: ... Glucosylceramide and galactosylceramide species were identified based on their retention times and MRM properties of commercially available reference standards (Avanti Polar Lipids, Birmingham, AL, USA). Quantification was performed using Skyline (v19.1 ...
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bioRxiv - Molecular Biology 2022Quote: ... in conjunction with referencing of appropriate internal standards: ceramide d18:1/17:0 and sphingomyelin d18:1/12:0 (Avanti Polar Lipids, Alabaster, AL, USA). Values were represented as mole fraction with respect to total lipid (% molarity) ...
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bioRxiv - Physiology 2024Quote: ... The homogenate was mixed with 1 mL of Extraction Buffer containing IPA/H2O/Ethyl Acetate (30:10:60, v/v/v) and Avanti Lipidomix Internal Standard (diluted 1:1000) (Avanti Polar Lipids, Inc. Alabaster, AL). Samples were vortexed and transferred to bead mill tubes for homogenization using a VWR Bead Mill at 6000 g for 30 seconds ...
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bioRxiv - Physiology 2024Quote: ... The homogenate was mixed with 1 mL of Extraction Buffer containing IPA/H2O/Ethyl Acetate (30:10:60, v/v/v) and Avanti Lipidomix Internal Standard (diluted 1:1000) (Avanti Polar Lipids, Inc. Alabaster, AL). Samples were vortexed and transferred to bead mill tubes for homogenization using a VWR Bead Mill at 6000 g for 30 seconds ...
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bioRxiv - Neuroscience 2024Quote: ... and 25 μL serum was precipitated using 75 μL of a precipitation solution made from isopropanol (IPA) supplemented with 14 isotopically labeled internal standards (IS) from Avanti Polar Lipids (Alabama, AL, USA). The IS included were cholesterol (d7) ...
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bioRxiv - Physiology 2024Quote: ... Plasma was mixed with 1 mL of Extraction Buffer containing IPA/H2O/Ethyl Acetate (30:10:60, v/v/v) and Avanti Lipidomix Internal Standard (diluted 1:1000) (Avanti Polar Lipids, Inc. Alabaster, AL). Samples were vortexed and transferred to bead mill tubes for homogenization using a VWR Bead Mill at 6000 g for 30 seconds ...
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bioRxiv - Molecular Biology 2023Quote: ... A quality control sample composed of all samples in the same ratio together with the SPLASH® Lipidomix® Mass Spec Standard (Avanti Polar Lipids, Inc., Alabaster USA) was created for subsequent lipid quantification and added in the MS run ...
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bioRxiv - Neuroscience 2023Quote: ... (1:3 (v/v)) was spiked with 0.5 µg/ml triacylglycerol (TAG 15:0/18:1-d7/15:0, Avanti Polar Lipids, 791648C), diacylglycerol (DAG 15:0/18:1-d7 ...
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bioRxiv - Cancer Biology 2020Quote: ... The media was changed to 0.5% Fatty-Acid Free BSA DMEM with either chloroform (ctrl) or 2.5 μM NBD-arachidonic acid (Avanti Polar Lipids 810106C). Media was made before being added to cells ...