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Citations for Avanti Polar Lipids :
101 - 142 of 142 citations for Recombinant Human CD19 protein His tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl) (sodium salt) (18:1 Biotinyl Cap PE) were purchased from Avanti Polar Lipids. 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl ...
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bioRxiv - Biophysics 2023Quote: ... and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl) (sodium salt) (18:1 Biotinyl Cap PE) were purchased from Avanti Polar Lipids. Texas Red-DHPE was purchased from AAT Bioquest ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Liss Rhod PE) were purchased from Avanti Polar Lipids. D-glucose ...
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bioRxiv - Biochemistry 2023Quote: ... PS 17:0-14:1, PE 17:0-20:4, PC 17:0-20:4, PI 17:0-20:4, LPE 17:1; Avanti Polar Lipids) was added before lipid extraction ...
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bioRxiv - Biophysics 2023Quote: ... 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE, 16:0/18:1 PE), cholesterol, brain octadecanoyl sphingomyelin (SM, 18:0) (Avanti Polar Lipids), Flipper-TR® 27 ...
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bioRxiv - Biophysics 2021Quote: ... Incorporation of native or denatured GlpG labeled with BtnPyr was measured using quenching of pyrene fluorescence from BtnPyr by dabcyl-DOPE (Avanti Polar Lipids) mixed at the 0.1% lipid-to-lipid molar ratio ...
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bioRxiv - Bioengineering 2023Quote: ... Individual lipid synthetic standards and EquiSPLASH containing equal concentrations of deuterium-labeled lipid standard (100 μg/mL) were purchased from Avanti Polar Lipids Inc ...
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bioRxiv - Molecular Biology 2023Quote: ... The dried samples were reconstituted using acetonitrile/isopropanol/water 65:30:5 containing stable isotope labeled internal lipid standards (Splash Lipidomix, Avanti Polar Lipids) prior to LC-MS analysis ...
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bioRxiv - Cell Biology 2023Quote: ... An equal volume of 2 μM Nitrobenzoxadiazole (NBD)-labeled lipids (Avanti Polar lipids: NBD-PC (18:1-06:0 NBD PC, 810132C); NBD-lyso-PC (12:0 lyso NBD PC ...
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bioRxiv - Biophysics 2019Quote: ... The second leaflet of the bilayer was assembled through Langmuir-Schaefer transfer after 1 mg/ml of DOPC with 0.2 % (mol) Cy5-PE (Avanti Polar Lipids, AL, USA) was applied to an air-water interface and kept at a surface pressure of 30 mN/m.
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bioRxiv - Neuroscience 2020Quote: ... and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3 benzoxadiazol-4-yl) (NBD-PE) were purchased from Avanti Polar Lipids (Alabaster, AL). The polystyrene beads (2.1 μm in diameter ...
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bioRxiv - Biophysics 2022Quote: ... sodium salt (PI), and L-α-phosphatidylethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (egg-transphosphatidylated, chicken) (Rh-PE) (all Avanti Polar Lipids) were mixed in chloroform at mol% of 75% ...
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bioRxiv - Microbiology 2020Quote: ... Lipid standards for mass spectrometry including 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (14:0/14:0-PE) and 1,2-dimyristoyl-sn-glycerol-3-phosphocholine (14:0/14:0-PC) were purchased from Avanti Polar Lipids (Alabaster, AL). All other reagents were purchased from Thermo Fisher Scientific (Hampton ...
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bioRxiv - Biophysics 2022Quote: ... and the lipophilic fluorescent probe 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rho-PE) were purchased from Avanti Polar Lipids (Alabaster, AL). Methanol and chloroform were from Fisher (Suwanee ...
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Antigen Geometry Tunes Mast Cell Signaling Through Distinct FcεRI Aggregation and Structural ChangesbioRxiv - Biophysics 2023Quote: ... 18:1 (Δ9-Cis) PC (DOPC) and 16:0 DNP Cap PE lipids were purchased from Avanti Polar Lipids (850375C and 810528C, respectively). DNP-Peg12-NHS ester was purchased from BroadPharm (BP-22397) ...
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bioRxiv - Cell Biology 2020Quote: ... 2 µl of 50 pmol/µl phosphoinositide containing 3.75 nmol mixed phospholipids (PC, PE, and PS in 45:35:15 molar ratio) (Echelon Biosciences and Avanti Polar Lipids, Alabaster, Alabama) in methanol:chloroform (9:1 v/v ...
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bioRxiv - Biophysics 2019Quote: ... 1-palmitoyl-2-hydroxy-sn-glycero-3-phosphocholine (16:0 lysoPC) and 1,2-dilinoleoyl-sn-glycero-3-phosphoethanolamine (18:2 PE, DLiPE) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Tripalmitin was a generous gift from M ...
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bioRxiv - Biophysics 2021Quote: ... and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rho-PE) were purchased from Avanti Polar Lipids (Alabaster, Alabama, USA) and used without further purification ...
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bioRxiv - Molecular Biology 2020Quote: ... Data were corrected for differences in extraction efficiency using the recovery of the DNP-PE internal standard (Avanti Polar Lipids, Inc., Alabaster, AL; USA).
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bioRxiv - Biophysics 2021Quote: ... 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rhod PE) and cholesterol were purchased from (Avanti Polar Lipids, Alabaster, Alabama, U.S.A.) Composition of myelin membrane - DOPC/BSM/DOPE/PI/DOPS/Cholesterol (4:3:1:1:0.4:0.6) ...
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bioRxiv - Molecular Biology 2020Quote: Lipids were extracted using a modified Bligh and Dyer protocol [79] with DNP-PE-C16:0/C16:0-DAG (2,4-dinitrophenyl phosphatidylethanolamine diacylglycerol; Avanti Polar Lipids, Inc., Alabaster, AL) used as an internal standard ...
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bioRxiv - Biophysics 2022Quote: ... (PE-Cy5.5) and 1-palmitoyl-2-(dipyrrometheneboron difluoride)-undecanoyl-sn-glycero-3-phosphocholine (TopFluor®-PC) were purchased from Avanti Polar Lipids (Alabama,USA). Atto647N-PI(4,5)P2 is a gift from Pr ...
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bioRxiv - Microbiology 2023Quote: ... Pigments were extracted using a modified Bligh and Dyer protocol (Popendorf et al., 2013) with DNP-PE-C16:0/C16:0-DAG (2,4-dinitrophenyl phosphatidylethanolamine diacylglycerol; Avanti Polar Lipids, Inc., Alabaster, AL) used as an internal standard ...
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bioRxiv - Cell Biology 2023Quote: ... doped with 2% SUV solution of 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-cap biotinyl (16:0 Biotinyl Cap PE) (Avanti Polar Lipids; cat no. 870277) on acid-cleaned coverslips ...
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bioRxiv - Physiology 2023Quote: The standards (PC_36:4 and PE_34:2) and the isotope labeled internal standards (PC_33:1-d7 and PE_31:1-d7) were purchased from Avanti Polar Lipids (Alabaster, Alabama, USA). The Standard solution at concentrations of 0 ...
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bioRxiv - Cell Biology 2022Quote: Prior to the lipid extraction samples were thawed on ice and spiked with SPLASH LIPIDOMIX deuterium-labeled internal standards (Avanti Polar Lipids Inc.). The lipids were extracted from samples using a modified Bligh and Dyer total lipid extraction method (Bligh and Dyer ...
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bioRxiv - Biochemistry 2023Quote: ... prior to denaturing intact protein mass spectrometry analysis the protein (2 mg mL-1) was incubated with 200 µM sphinganine (Avanti Polar Lipids), 200 µM fumonisin B1 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... BMP (bis(monooleoylglycero)phosphate (S,R Isomer)) and lyso-PC (lauroyl-lysoPC) were obtained from Avanti Polar Lipids (Alabaster, AL). Chlorpromazine was purchased from Sigma (Cat# C8138) ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Liss Rhod PE) were obtained from Avanti Polar Lipids (Alabaster, AL, USA), Streptavidin from IBA-Lifesciences (Göttingen ...
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bioRxiv - Biophysics 2023Quote: ... Small unilamellar vesicles are prepared by drying a mixture of 93% POPC and 7% 18:1 biotinyl-PE (Avanti Polar Lipids, catalog #850457C and 870282C) under compressed nitrogen gas followed by overnight drying under vacuum ...
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bioRxiv - Microbiology 2024Quote: 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) and 18:1 Biotinyl Cap PE (DOPE-Cap-B) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Small unilamellar vesicles (SUVs ...
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bioRxiv - Cell Biology 2022Quote: ... N-(2’-(R)-hydroxypalmitoyl(d9))-D-ribo-phytosphingosine (d9-C16:0 Cer-C) (all purchased from Avanti Polar Lipids, Alabaster, AL)] were used (5 pmol each) ...
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bioRxiv - Physiology 2021Quote: ... sphingosine-1-phosphate (Huzzah S1P, Human Serum Albumin/sphingosine-1-phosphate Complex; Avanti Polar Lipids, Inc., Alabaster, AL, USA) and other active reagents were added to the perfusion medium.
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bioRxiv - Cell Biology 2023Quote: ... and irreversibly bound fractions were each suspended in 300 µL of 1 M KOH in 95% CH3OH containing 2 µL of 1 µM N-(2’-(R)-hydroxypalmitoyl(d9)) D-erythro-sphingosine (Avanti Polar Lipids, Alabaster, AL, USA) as an internal standard and incubated at 60 °C for 1 h to release ω-OH ceramides from protein-bound ceramides ...
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bioRxiv - Cancer Biology 2020Quote: ... coli total lipid extract for the reconstitution of proteins was from Avanti Polar Lipids, Inc ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 μL of human fluid sample was precipitated with 190 μL of isopropanol containing 1,2-didodecanoyl-sn-glycero-3-phosphocholine (Avanti Polar Lipids) as an internal standard ...
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bioRxiv - Biochemistry 2022Quote: Purification and reconstitution of the protein in d54-DMPC and d22-DHPC (Avanti Polar Lipids) q=0.33 bicelles was performed as exactly described previously 46.
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bioRxiv - Biochemistry 2023Quote: ... and the protein was mixed with detergent solubilized SBPC (soybean phosphatidylcholine from Avanti polar lipids) using a molar ratio of 1:80 and incubated for 30 minutes at room temperature (RT) ...
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bioRxiv - Biochemistry 2024Quote: ... Purified protein was reconstituted into proteoliposomes (E. coli polar lipid extract, Avanti Polar Lipids, Alabaster, AL) by dialysis in 100 mM KCl ...
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bioRxiv - Biochemistry 2023Quote: ... Ceramidase activity was assessed by incubating 1.8 μM of purified SIDT1 and SIDT2 proteins with 40 μM ceramide (d18:1/18:0) (Cat# 860518P, Avanti Polar Lipids) at room temperature for 30 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... lipid contents were extracted using the tert-butyl-methyl ether and methanol method47 from purified MBOAT7 proteins and lipid controls (Avanti Polar Lipids). Extracted lipids were resolubilized in 300 μL of isopropanol ...
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bioRxiv - Biochemistry 2021Quote: ... The reconstitution was done with a 1:4:130 molar ratio of AtAtm3: membrane scaffolding protein (MSP) 1D1: 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) (Avanti Polar Lipids, AL). After overnight incubation at 4 °C ...